Skip to main content
Plant Physiology logoLink to Plant Physiology
. 1979 Oct;64(4):630–634. doi: 10.1104/pp.64.4.630

Developmental Changes in the Activity of Messenger RNA Isolated from Germinating Castor Bean Endosperm 1

Lynne M Roberts a, J Michael Lord a
PMCID: PMC543150  PMID: 16661022

Abstract

The capacity of polyadenylated RNA from developing castor bean endosperm to program protein synthesis in a wheat germ cell-free translational system has been examined. Although the use of micrococcal nuclease-treated wheat germ extracts demonstrated a low but significant content of translatable mRNA in dry seeds, a large scale increase in total translational capacity was observed during germination. The cellular content of translatable mRNA peaked on the 4th day of germination and subsequently declined. It is concluded that protein synthesis in castor bean endosperm cells during germination is directed by newly transcribed mRNA.

Full text

PDF
633

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BEEVERS H. Metabolic production of sucrose from fat. Nature. 1961 Jul 29;191:433–436. doi: 10.1038/191433a0. [DOI] [PubMed] [Google Scholar]
  2. Beevers H. Glyoxysomes of castor bean endosperm and their relation to gluconeogenesis. Ann N Y Acad Sci. 1969 Dec 19;168(2):313–324. doi: 10.1111/j.1749-6632.1969.tb43118.x. [DOI] [PubMed] [Google Scholar]
  3. Bonner W. M., Laskey R. A. A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels. Eur J Biochem. 1974 Jul 1;46(1):83–88. doi: 10.1111/j.1432-1033.1974.tb03599.x. [DOI] [PubMed] [Google Scholar]
  4. Bowden L., Lord J. M. The cellular origin of glyoxysomal proteins in germinating castor-bean endosperm. Biochem J. 1976 Feb 15;154(2):501–506. doi: 10.1042/bj1540501. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Brooker J. D., Tomaszewski M., Marcus A. Preformed Messenger RNAs and Early Wheat Embryo Germination. Plant Physiol. 1978 Feb;61(2):145–149. doi: 10.1104/pp.61.2.145. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gerhardt B. P., Beevers H. Developmental studies on glyoxysomes in Ricinus endosperm. J Cell Biol. 1970 Jan;44(1):94–102. doi: 10.1083/jcb.44.1.94. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Marcus A. Seed germination and the capacity for protein synthesis. Symp Soc Exp Biol. 1969;23:143–160. [PubMed] [Google Scholar]
  8. Roberts B. E., Paterson B. M. Efficient translation of tobacco mosaic virus RNA and rabbit globin 9S RNA in a cell-free system from commercial wheat germ. Proc Natl Acad Sci U S A. 1973 Aug;70(8):2330–2334. doi: 10.1073/pnas.70.8.2330. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Speigel S., Marcus A. Polyribosome formation in early wheat embryo germination independent of either transcription or polyadenylation. Nature. 1975 Jul 17;256(5514):228–230. doi: 10.1038/256228a0. [DOI] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES