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. 1977 May;59(5):868–872. doi: 10.1104/pp.59.5.868

Effects of Light, Carbon Dioxide, and Temperature on Photosynthesis, Oxygen Inhibition of Photosynthesis, and Transpiration in Solanum tuberosum1

Sun-Ben Ku a,2, Gerald E Edwards a,2, Champ B Tanner a,3
PMCID: PMC543312  PMID: 16659958

Abstract

Individual leaves of potato (Solanum tuberosum L. W729R), a C3 plant, were subjected to various irradiances (400-700 nm), CO2 levels, and temperatures in a controlled-environment chamber. As irradiance increased, stomatal and mesophyll resistance exerted a strong and some-what paralleled regulation of photosynthesis as both showed a similar decrease reaching a minimum at about 85 neinsteins·cm−2·sec−1 (about ½ of full sunlight). Also, there was a proportional hyperbolic increase in transpiration and photosynthesis with increasing irradiance up to 85 neinsteins·cm−2·sec−1. These results contrast with many C3 plants that have a near full opening of stomata at much less light than is required for saturation of photosynthesis.

Inhibition of photosynthesis by 21% O2 was nearly overcome by a 2-fold increase in atmospheric levels of CO2 (about 1,200 ng·cm−3). Photosynthesis at 25 C, high irradiance, 2.5% O2 and atmospheric levels of CO2 was about 80% of the CO2-saturated rate, suggesting that CO2 can be rate-limiting even without O2 inhibition of photosynthesis. With increasing CO2 concentration, mesophyll resistance decreased slightly while stomatal resistance increased markedly above 550 ng·cm−3 which resulted in a significant reduction in transpiration.

Although potato is a very productive C3 crop, there is substantial O2 inhibition of photosynthesis. The level of O2 inhibition was maximum around 25 C but the percentage inhibition of photosynthesis by O2 increased steadily from 38% at 16 C to 56% at 36 C. Photosynthesis and transpiration showed broad temperature optima (16-25 C). At higher temperatures, both the increased percentage inhibition of photosynthesis by O2 and the increased stomatal resistance limit photosynthesis, while increased stomatal resistance limits transpiration. Water use efficiency, when considered at a constant vapor pressure gradient, increased with increasing irradiance, CO2 concentration, and temperature.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Andrews T. J., Lorimer G. H., Tolbert N. E. Incorporation of molecular oxygen into glycine and serine during photorespiration in spinach leaves. Biochemistry. 1971 Dec 7;10(25):4777–4782. doi: 10.1021/bi00801a027. [DOI] [PubMed] [Google Scholar]
  2. Badger M. R., Andrews T. J. Effects of CO2, O2 and temperature on a high-affinity form of ribulose diphosphate carboxylase-oxygenase from spinach. Biochem Biophys Res Commun. 1974 Sep 9;60(1):204–210. doi: 10.1016/0006-291x(74)90192-2. [DOI] [PubMed] [Google Scholar]
  3. Chapman H. W., Loomis W. E. Photosynthesis in the Potato Under Field Conditions. Plant Physiol. 1953 Oct;28(4):703–716. doi: 10.1104/pp.28.4.703. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hew C. S., Krotkov G., Canvin D. T. Effects of Temperature on Photosynthesis and CO(2) Evolution in Light and Darkness by Green Leaves. Plant Physiol. 1969 May;44(5):671–677. doi: 10.1104/pp.44.5.671. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hsiao T. C., Allaway W. G. Action Spectra for Guard Cell Rb Uptake and Stomatal Opening in Vivia faba. Plant Physiol. 1973 Jan;51(1):82–88. doi: 10.1104/pp.51.1.82. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Jolliffe P. A., Tregunna E. B. Effect of Temperature, CO(2) Concentration, and Light Intensity on Oxygen Inhibition of Photosynthesis in Wheat Leaves. Plant Physiol. 1968 Jun;43(6):902–906. doi: 10.1104/pp.43.6.902. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Laing W. A. Regulation of Soybean Net Photosynthetic CO(2) Fixation by the Interaction of CO(2), O(2), and Ribulose 1,5-Diphosphate Carboxylase. Plant Physiol. 1974 Nov;54(5):678–685. doi: 10.1104/pp.54.5.678. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Ogren W. L., Bowes G. Ribulose diphosphate carboxylase regulates soybean photorespiration. Nat New Biol. 1971 Mar 31;230(13):159–160. doi: 10.1038/newbio230159a0. [DOI] [PubMed] [Google Scholar]
  9. Zelitch I. Improving the efficiency of photosynthesis. Science. 1975 May 9;188(4188):626–633. doi: 10.1126/science.188.4188.626. [DOI] [PubMed] [Google Scholar]

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