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. 1967 Jan;15(1):186–190. doi: 10.1128/am.15.1.186-190.1967

Production of Aflatoxin on Wheat and Oats: Measurement with a Recording Densitometer

R D Stubblefield 1, O L Shotwell 1, C W Hesseltine 1, M L Smith 1, H H Hall 1
PMCID: PMC546866  PMID: 6031432

Abstract

A method has been developed for the production of aflatoxin by growing Aspergillus flavus NRRL 3145 on solid substrate wheat. Optimal yields of 900 μg of aflatoxin G1 and 900 μg of aflatoxin B1 per g of substrate were obtained in 4 to 5 days at 28 C. A study of aflatoxin production on hulls and groats of oats and on whole oats by A. flavus strains NRRL 2999, NRRL 3000, and NRRL 3145 revealed that aflatoxin was produced on all three substrates, although production was very slight on hulls. Strain NRRL 3145 grown on solid substrate groats produced the largest amounts of aflatoxin: 580 μg of B1 and 450 μg of G1 per g of substrate. A densitometric method for reading thin-layer chromatographic plates is described; this is more objective and more accurate than the visual methods previously used for the determination of all four aflatoxins.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ayres J. L., Sinnhuber R. O. Fluorodensitometry of aflatoxin on thin-layer plates. J Am Oil Chem Soc. 1966 Jun;43(6):423–424. doi: 10.1007/BF02646806. [DOI] [PubMed] [Google Scholar]
  2. CHANG S. B., ABDEL-KADER M. M., WICK E. L., WOGAN G. N. AFLATOXIN B2: CHEMICAL IDENTITY AND BIOLOGICAL ACTIVITY. Science. 1963 Nov 29;142(3596):1191–1192. doi: 10.1126/science.142.3596.1191. [DOI] [PubMed] [Google Scholar]
  3. Coomes T. J., Crowther P. C., Francis B. J., Stevens L. The detection and estimation of aflatoxin in groundnuts and groundnut materials. IV. Routine assessment of toxicity due to aflatoxin B1. Analyst. 1965 Aug;90(73):492–496. doi: 10.1039/an9659000492. [DOI] [PubMed] [Google Scholar]
  4. HESSELTINE C. W. A MILLENNIUM OF FUNGI, FOOD, AND FERMENTATION. Mycologia. 1965 Mar-Apr;57:149–197. [PubMed] [Google Scholar]
  5. Hesseltine C. W., Shotwell O. L., Ellis J. J., Stubblefield R. D. Aflatoxin formation by Aspergillus flavus. Bacteriol Rev. 1966 Dec;30(4):795–805. doi: 10.1128/br.30.4.795-805.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. PONS W. A., Jr, GOLDBLATT L. A. THE DETERMINATION OF AFLATOXINS IN COTTONSEED PRODUCTS. J Am Oil Chem Soc. 1965 Jun;42:471–475. doi: 10.1007/BF02540087. [DOI] [PubMed] [Google Scholar]
  7. Shotwell O. L., Hesseltine C. W., Stubblefield R. D., Sorenson W. G. Production of aflatoxin on rice. Appl Microbiol. 1966 May;14(3):425–428. doi: 10.1128/am.14.3.425-428.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]

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