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. 1967 Mar;15(2):390–397. doi: 10.1128/am.15.2.390-397.1967

Characterization of Clostridia by Gas Chromatography

I. Differentiation of Species by Cellular Fatty Acids

C Wayne Moss 1, Vester J Lewis 1
PMCID: PMC546910  PMID: 4291511

Abstract

Fatty acids of 41 strains representing 13 species of Clostridium were extracted directly from whole cells and examined as methyl esters by gas-liquid chromatography. Both visual and quantitative comparisons of the resulting chromatograms for the presence and relative amounts of large major peaks allowed rapid differentiation of C. perfringens, C. sporogenes, and C. bifermentans from each other and from 10 other species. Each of the three former species possessed a different characteristic fatty acid methyl ester profile that was exhibited by all strains tested within the respective species. Culture age and growth media influenced the relative proportions of certain of the acids, but such differences did not limit species differentiation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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