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. 1967 Jul;15(4):866–870. doi: 10.1128/am.15.4.866-870.1967

Nutritional Requirements of Staphylococcus aureus S-61

Robert A Mah 1, Daniel Y C Fung 1, Stephen A Morse 1
PMCID: PMC547084  PMID: 6049306

Abstract

A synthetic medium was devised for growth of Staphylococcus aureus strain S-6. The growth yield in synthetic medium was compared to that in complex medium containing an equivalent amount of protein hydrolysate. Enterotoxin B formation in the two media was also compared. The defined medium was composed of inorganic salts, 11 amino acids (glycine, valine, leucine, threonine, phenylalanine, tyrosine, cysteine, methionine, proline, arginine, and histidine), and three vitamins (thiamine, nicotinic acid, and biotin). Biotin was a growth factor requirement of S-6 when glutamic acid but not glucose was used as a carbon source. The quantity of enterotoxin B produced in the defined medium was about one-seventh of that produced in complex medium, even though the growth yields were similar.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BERGDOLL M. S., SUGIYAMA H., DACK G. M. Staphylococcal enterotoxin. I. Purification. Arch Biochem Biophys. 1959 Nov;85:62–69. doi: 10.1016/0003-9861(59)90447-3. [DOI] [PubMed] [Google Scholar]
  2. Bergdoll M. S., Chu F. S., Huang I. Y., Rowe C., Shih T. Staphylococcal enterotoxin B. 3. The physicochemical properties and the N- and C-terminal amino acid sequences. Arch Biochem Biophys. 1965 Oct;112(1):104–110. doi: 10.1016/0003-9861(65)90016-0. [DOI] [PubMed] [Google Scholar]
  3. CASMAN E. P., BENNETT R. W. CULTURE MEDIUM FOR THE PRODUCTION OF STAPHYLOCOCCAL ENTEROTOXIN A. J Bacteriol. 1963 Jul;86:18–23. doi: 10.1128/jb.86.1.18-23.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Favorite G. O., Hammon W. M. The Production of Staphylococcus Enterotoxin and Alpha Hemolysin in a Simplified Medium. J Bacteriol. 1941 Mar;41(3):305–316. doi: 10.1128/jb.41.3.305-316.1941. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. GALE E. F. Assimilation of amino acids by Gram-positive bacteria and some actions of antibiotics thereon. Adv Protein Chem. 1953;8:285–391. doi: 10.1016/s0065-3233(08)60094-7. [DOI] [PubMed] [Google Scholar]
  6. GRETLER A. C., MUCCIOLO P., EVANS J. B., NIVEN C. F., Jr Vitamin nutrition of the staphylococci with special reference to their biotin requirements. J Bacteriol. 1955 Jul;70(1):44–49. doi: 10.1128/jb.70.1.44-49.1955. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Ivler D. Comparative metabolism of virulent and avirulent staphylococci. Ann N Y Acad Sci. 1965 Jul 23;128(1):62–80. doi: 10.1111/j.1749-6632.1965.tb11630.x. [DOI] [PubMed] [Google Scholar]
  8. Morse S. A., Mah R. A. Microtiter hemagglutination-inhibition assay for staphylococcal enterotoxin B. Appl Microbiol. 1967 Jan;15(1):58–61. doi: 10.1128/am.15.1.58-61.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. RAMSEY H. H. Endogenous respiration of Staphylococcus aureus. J Bacteriol. 1962 Mar;83:507–514. doi: 10.1128/jb.83.3.507-514.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. SURGALLA M. J., KADAVY J. L., BERGDOLL M. S., DACK G. M. Staphylococcal enterotoxin: production methods. J Infect Dis. 1951 Sep-Oct;89(2):180–184. doi: 10.1093/infdis/89.2.180. [DOI] [PubMed] [Google Scholar]
  11. Schantz E. J., Roessler W. G., Wagman J., Spero L., Dunnery D. A., Bergdoll M. S. Purification of staphylococcal enterotoxin B. Biochemistry. 1965 Jun;4(6):1011–1016. doi: 10.1021/bi00882a005. [DOI] [PubMed] [Google Scholar]
  12. Spero L., Stefanye D., Brecher P. I., Jacoby H. M., Dalidowicz J. E., Schantz E. J. Amino acid composition and terminal amino acids of staphylococcal enterotoxin B. Biochemistry. 1965 Jun;4(6):1024–1030. doi: 10.1021/bi00882a007. [DOI] [PubMed] [Google Scholar]
  13. Weirether F. J., Lewis E. E., Rosenwald A. J., Lincoln R. E. Rapid quantitative serological assay of staphylococcal enterotoxin B. Appl Microbiol. 1966 Mar;14(2):284–291. doi: 10.1128/am.14.2.284-291.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]

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