Abstract
A modified sporulation medium for Clostridium perfringens was formulated in which a larger number of spores were produced than in SEC broth and in which spores of greater heat resistance were produced than in Ellner's medium when it was also used as the suspending medium. This modified medium consisted of 1.5% peptone; 3.0% Trypticase; 0.4% starch; 0.5% NaCl; and 0.02% MgSO4. The addition of 0.1% sodium thioglycolate and 0.0001% thiamine hydrochloride was optional. The optimal temperature for sporulation of five strains was 37 C in comparison with 5, 22, and 46 C. Sporulation had occurred by 6 hr and was essentially complete after 20 hr at 37 C. Noyes veal broth without glucose also supported the formation of heat-resistant spores but in smaller numbers than did the modified medium. Very low numbers of spores, or none, were produced under the same conditions in pea or tuna slurries.
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- ANGELOTTI R., HALL H. E., FOTER M. J., LEWIS K. H. Quantitation of Clostridium perfringens in foods. Appl Microbiol. 1962 May;10:193–199. doi: 10.1128/am.10.3.193-199.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
- ELLNER P. D. A medium promoting rapid quantitative sporulation in Clostridium perfringens. J Bacteriol. 1956 Apr;71(4):495–496. doi: 10.1128/jb.71.4.495-496.1956. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Groom R. A., Strong D. H. Sporulation of Clostridium perfringens (welchii) in four laboratory media. J Appl Bacteriol. 1966 Aug;29(2):308–318. doi: 10.1111/j.1365-2672.1966.tb03481.x. [DOI] [PubMed] [Google Scholar]
- HALL H. E., ANGELOTTI R., LEWIS K. H., FOTER M. J. CHARACTERISTICS OF CLOSTRIDIUM PERFRINGENS STRAINS ASSOCIATED WITH FOOD AND FOOD-BORNE DISEASE. J Bacteriol. 1963 May;85:1094–1103. doi: 10.1128/jb.85.5.1094-1103.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
- YAMAGISHI T., ISHIDA S., NISHIDA S. ISOLATION OF TOXIGENIC STRAINS OF CLOSTRIDIUM PERFRINGENS FROM SOIL. J Bacteriol. 1964 Sep;88:646–652. doi: 10.1128/jb.88.3.646-652.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
- ZOHA S. M., SADOFF H. L. Production of spores by a putrefactive anaerobe. J Bacteriol. 1958 Aug;76(2):203–206. doi: 10.1128/jb.76.2.203-206.1958. [DOI] [PMC free article] [PubMed] [Google Scholar]