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. 1967 Sep;15(5):1114–1121. doi: 10.1128/am.15.5.1114-1121.1967

Permanent Turbidity-Standards

William G Roessler 1,2, Carl R Brewer 1,2,1
PMCID: PMC547151  PMID: 6077410

Abstract

Permanent turbidity reference standards suitable for measurement of microbial suspensions were prepared by suspending finely divided titanium dioxide in aryl sulfonamide-formaldehyde or methylstyrene resins. Turbidities of these standards, adjusted to a useful range for microbiological and immunological studies, were compared with other reference standards in use today. Tube holders for a Coleman Photonephelometer and a Nepho-Colorimeter were modified to eliminate the water well and to allow use of optically standardized 10-, 16-, or 18-mm test tubes. The standards and the tube holders have been used satisfactorily for more than 12 years.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brewer J. H., Cook E. B. A Permanent Nephelometer from Pyrex Glass. Am J Public Health Nations Health. 1939 Oct;29(10):1147–1148. doi: 10.2105/ajph.29.10.1147. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. ELLINGHAUSEN H. C., Jr, MCCULLOUGH W. G. NUTRITION OF LEPTOSPIRA POMONA AND GROWTH OF 13 OTHER SEROTYPES: A SERUM-FREE MEDIUM EMPLOYING OLEIC ALBUMIN COMPLEX. Am J Vet Res. 1965 Jan;26:39–44. [PubMed] [Google Scholar]
  3. GAVIN J. J. Microbiological process report: analytical microbiology. III. Turbidimetric methods. Appl Microbiol. 1957 Jul;5(4):235–243. doi: 10.1128/am.5.4.235-243.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. HASLAM J., SQUIRRELL D. C. M. The preparation of permanent turbidimetric standards for use in the determination of small amounts of chloride. Biochem J. 1951 Jan;48(1):48–50. doi: 10.1042/bj0480048. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. HIGUCHI K., CARLIN C. E. Studies on the nutrition and physiology of Pasteurella pestis. I. A casein hydrolyzate medium for the growth of Pasteurella pestis. J Bacteriol. 1957 Jan;73(1):122–129. doi: 10.1128/jb.73.1.122-129.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. HIGUCHI K., CARLIN C. E. Studies on the nutrition and physiology of Pasteurella pestis. II. A defined medium for the growth of Pasteurella pestis. J Bacteriol. 1958 Apr;75(4):409–413. doi: 10.1128/jb.75.4.409-413.1958. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hallinan F. J. Pyrex Suspensions in Turbidimetric and Colorimetric Determinations-Standards of Comparison for Bacterial Suspensions and in the Resazurin Test. Am J Public Health Nations Health. 1943 Feb;33(2):137–140. doi: 10.2105/ajph.33.2.137. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. JOHNSON R. C., GARY N. D. NUTRITION OF LEPTOSPIRA POMONA. II. FATTY ACID REQUIREMENTS. J Bacteriol. 1963 May;85:976–982. doi: 10.1128/jb.85.5.976-982.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. JOHNSON R. C., GARY N. D. NUTRITION OF LEPTOSPIRA POMONA. III. CALCIUM, MAGNESIUM, AND POTASSIUM REQUIREMENTS. J Bacteriol. 1963 May;85:983–985. doi: 10.1128/jb.85.5.983-985.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. JOHNSON R. C., GARY N. D. Nutrition of Leptospira pomona. 1. A chemically defined substitute for rabbit serum ultrafiltrate. J Bacteriol. 1962 Mar;83:668–672. doi: 10.1128/jb.83.3.668-672.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. JOHNSON R. C., ROGERS P. 5-FLUOROURACIL AS A SELECTIVE AGENT FOR GROWTH OF LEPTOSPIRAE. J Bacteriol. 1964 Feb;87:422–426. doi: 10.1128/jb.87.2.422-426.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. JOHNSON R. C., ROGERS P. DIFFERENTIATION OF PATHOGENIC AND SAPROPHYTIC LEPTOSPIRES WITH 8-AZAGUANINE. J Bacteriol. 1964 Dec;88:1618–1623. doi: 10.1128/jb.88.6.1618-1623.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. JOHNSON R. C., ROGERS P. METABOLISM OF LEPTOSPIRAE. I. UTILIZATION OF AMINO ACIDS AND PURINE, AND PYRIMIDINE BASES. Arch Biochem Biophys. 1964 Sep;107:459–470. doi: 10.1016/0003-9861(64)90302-9. [DOI] [PubMed] [Google Scholar]
  14. MAALØE O. The International Reference Preparation for Opacity; notes and description. Bull World Health Organ. 1955;12(5):769–775. [PMC free article] [PubMed] [Google Scholar]
  15. NAGLE S. C., Jr, ANDERSON R. E., GARY N. D. Chemically defined medium for the growth of Pasteurella tularensis. J Bacteriol. 1960 Apr;79:566–571. doi: 10.1128/jb.79.4.566-571.1960. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. SANDERS T. H., HIGUCHI K., BREWER C. R. Studies on the nutrition of Brucella melitensis. J Bacteriol. 1953 Sep;66(3):294–299. doi: 10.1128/jb.66.3.294-299.1953. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. TIETZE F., NEURATH H. Light scattering studies on insulin; the minimum molecular weight of insulin. J Biol Chem. 1952 Jan;194(1):1–13. [PubMed] [Google Scholar]
  18. VAS K. Turbidimetric measurement of microbial density. Acta Microbiol Acad Sci Hung. 1955;2(3):203–213. [PubMed] [Google Scholar]

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