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. 1968 Jan;16(1):21–24. doi: 10.1128/am.16.1.21-24.1968

Detection of Botulinal Toxins by Immunodiffusion1

Barry L Vermilyea 1, Homer W Walker 1, John C Ayres 1
PMCID: PMC547297  PMID: 4965915

Abstract

A procedure employing concentration with Sephadex and analysis by gel diffusion (Ouchterlony) was used to detect the toxins of Clostridium botulinum in foods. Botulinal toxins with toxic levels of 370 to 557 mouse LD50 per milliliter were detected in the food samples. Test results were verified by use of the mouse protection test. Approximately 24 hr were required to complete the entire procedure.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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