Abstract
A method previously reported for detecting virus in a model system composed of cottage cheese contaminated with coxsackievirus type A9 has been adapted to detecting selected strains of enteroviruses in a variety of foods. Bentonite is omitted and serum is added for extracting virus from low-protein foods. Samples of foods, usually 25 g, must contain at least 3 to 4 plaque-forming units for a 50% probability of detecting virus. Sensitivity in detecting echovirus type 6 was lower than that for the other viruses used. After extraction from potato salad, poliovirus type 2 was completely reactivated if it had been neutralized with coproantibody, but it was only partially reactivated if neutralized with hyperimmune rabbit serum.
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- CLIVER D. O., YEATMAN J. ULTRACENTRIFUGATION IN THE CONCENTRATION AND DETECTION OF ENTEROVIRUSES. Appl Microbiol. 1965 May;13:387–392. doi: 10.1128/am.13.3.387-392.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cliver D. O., Anders R. J. Apparent "double mutation" induced by gamma rays. Nature. 1968 Apr 13;218(5137):187–188. doi: 10.1038/218187a0. [DOI] [PubMed] [Google Scholar]
- Cliver D. O. Food-associated viruses. Health Lab Sci. 1967 Oct;4(4):213–221. [PubMed] [Google Scholar]
- Heremans J. F., Crabbé P. A., Masson P. L. Biological significance of exocrine gamma-A-immunoglobulin. Acta Med Scand Suppl. 1966;445:84–88. doi: 10.1111/j.0954-6820.1966.tb02343.x. [DOI] [PubMed] [Google Scholar]
- Herrmann J. E., Cliver D. O. Food-borne virus:detection in a model system. Appl Microbiol. 1968 Apr;16(4):595–602. doi: 10.1128/am.16.4.595-602.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- RAPPAPORT C. Trypsinization of monkey-kidney tissue: an automatic method for the preparation of cell suspensions. Bull World Health Organ. 1956;14(1):147–166. [PMC free article] [PubMed] [Google Scholar]