Skip to main content
PMC home page
Nature Communications logoLink to Nature Communications
. 2017 May 9;8:15281. doi: 10.1038/ncomms15281

Regulation of cardiomyocyte behavior in zebrafish trabeculation by Neuregulin 2a signaling

S Javad Rasouli 1, Didier Y R Stainier 1,a
PMCID: PMC5477525  PMID: 28485381

Abstract

Trabeculation is crucial for cardiac muscle growth in vertebrates. This process requires the Erbb2/4 ligand Neuregulin (Nrg), secreted by the endocardium, as well as blood flow/cardiac contractility. Here, we address two fundamental, yet unresolved, questions about cardiac trabeculation: why does it initially occur in the ventricle and not the atrium, and how is it modulated by blood flow/contractility. Using loss-of-function approaches, we first show that zebrafish Nrg2a is required for trabeculation, and using a protein-trap line, find that it is expressed in both cardiac chambers albeit with different spatiotemporal patterns. Through gain-of-function experiments, we show that atrial cardiomyocytes can also respond to Nrg2a signalling, suggesting that the cardiac jelly, which remains prominent in the atrium, represents a barrier to Erbb2/4 activation. Furthermore, we find that blood flow/contractility is required for Nrg2a expression, and that while non-contractile hearts fail to trabeculate, non-contractile cardiomyocytes are also competent to respond to Nrg2a/Erbb2 signalling.

Cardiac trabeculae (which are sponge-like muscular structures) form mostly as a result of cardiomyocyte (CM) delamination in zebrafish. Here, the authors identify Nrg2a in zebrafish as a key regulator of trabeculation, and atrial and non-contractile CMs also respond to Nrg2a despite not forming trabeculae.

Cardiac trabeculation, which allows the increase in muscle mass prior to the formation of coronaries, is one of the most important processes the vertebrate heart must undergo to form and function properly1. During this process, trabeculae, which are sponge-like muscular structures in the cardiac cavity, form as a result of cardiomyocyte delamination and proliferation2,3,4,5,6. Defects in this complex morphogenetic event lead to embryonic lethality or adult onset dilated cardiomyopathy5,7,8,9,10,11,12 illustrating the importance of trabeculation in cardiac development and function.

The vertebrate heart initially consists of two cell layers, the endocardium and myocardium, separated by an acellular, gelatinous matrix called the cardiac jelly13,14,15. Ablating components of the cardiac jelly by injecting hyaluronidase in chick embryos has been reported to cause looping defects16, indicating that it plays an important role during early cardiac morphogenesis. Endocardial–myocardial interactions are known to orchestrate cardiac trabeculation; for example, in chick embryos, a myocardial-derived growth factor, Angiopoietin 1, has been reported to bind to its receptor Tie2 which is expressed by endocardial cells and modify their angiogenic behaviour which in turn affects the complexity of cardiac trabeculation17. Similarly, signalling from endocardial to myocardial cells is also necessary for trabeculation. For instance, mice lacking the endocardial-derived ligand Nrg1 or its receptors Erbb2 and Erbb4, which are expressed by myocardial cells, exhibit severe trabeculation defects7,8,12,18. Similarly, zebrafish erbb2 mutants fail to form trabeculae5. Additional studies have shown that Nrg signalling can positively regulate cardiomyocyte proliferation and heart regeneration19,20,21,22.

Cardiac formation can also be influenced by its function23,24,25. Recent studies have shown the dependence of trabeculation on physical forces generated by cardiac contractility and blood flow6,26,27. For instance, several groups have reported that reduction of blood flow in the ventricular chamber markedly diminishes trabeculation in chick embryos and zebrafish larvae6,26,28,29. By manipulating cardiac contractility, another study presented data indicating that cardiac contraction promotes trabeculation through the regulation of Notch signalling in the ventricular endocardium27. These and other findings support the hypothesis that physical forces are necessary in the developing heart, particularly during trabecular formation.

In this study, we use the zebrafish model to address two fundamental questions pertinent to cardiac trabeculation; first, why trabeculae initially form in the ventricle and not the atrium, as is observed in zebrafish as well as in other vertebrates including amniotes3,30, and second, how trabeculation is modulated by physical forces. Using a genetic approach and high-resolution live imaging, we first show that Nrg2a is an essential ligand for cardiac trabeculation in zebrafish. By ectopically expressing nrg2a in cardiomyocytes, we further show that atrial cardiomyocytes, like ventricular cardiomyocytes, can respond to Nrg2a/Erbb2 signalling. Using loss- and gain-of-function approaches, we show that blood flow/contractility modulates endocardial nrg2a expression while it is not required for the expression or function of Nrg2a/Erbb2 signalling effectors in cardiomyocytes.

Results

Nrg2a is required for cardiac trabeculation in zebrafish

Given the role of Nrg1 in cardiac trabeculation in mouse7,8,12,18, we mutated zebrafish nrg1 using TALEN technology31. Since nrg1 has multiple isoforms32, we targeted exon 2 which encodes part of the highly conserved IGc2-domain, and identified a Δ14 allele (Supplementary Fig. 1a) encoding a predicted truncated protein (Supplementary Fig. 1b) due to harbouring a premature stop codon within the same exon. Using brightfield microscopy, no gross morphological defects were observed in nrg1 Δ14 (nrg1bns101) homozygous mutant larvae (Supplementary Fig. 1c,d). Incrossing nrg1bns101/+ fish in the Tg(myl7:LIFEACT-GFP) background, a myocardial specific line in which F-actin (filamentous actin) is labelled with GFP33, we observed that trabeculation appears unaffected in nrg1bns101 mutant larvae (Supplementary Fig. 1e–h), which can survive to become fertile adults.

Next, we decided to investigate the role of Nrg2a, another member of the Nrg family, by using a conditional protein-trap line, nrg2amn0237Gt (refs 34, 35). Using brightfield microscopy, nrg2a mutants can be recognized by their aberrant jaws (Fig. 1a,b) as well as other defects in median fin fold (MFF) morphogenesis and swim bladder inflation35. As previously reported, the gene-breaking transgene (GBT) nrg2a allele generates a truncated Nrg2a protein tagged with mRFP (Nrg2a-mRFP) due to the in-frame integration of the mutagenic pGBT-RP2.1 (RP2) cassette in the intron between alternative exon 1C and exon 2 (ref. 35). Prior studies have also shown that the expression of Nrg2a-mRFP recapitulates that of endogenous nrg2a (refs 34, 35). Confocal imaging of larvae from nrg2a+/− incrosses in the Tg(myl7:LIFEACT-GFP) background (Fig. 1c–k) revealed that Nrg2a-mRFP is highly expressed in the endocardium at early larval stages (Fig. 1d,e,g,h,j,k), and that nrg2a−/− hearts fail to form trabeculae by 75 h post fertilization (hpf) (Fig. 1c–e). Observation of nrg2a mutants at later stages, including 120 and 168 hpf (Fig. 1f–k), confirmed that their trabeculation defects appear as severe as those in erbb2 mutants5. Overall, these data suggest that Nrg2a, and not Nrg1, is the crucial regulator of cardiac trabeculation in zebrafish.

Figure 1. Nrg2a is required for cardiac trabeculation in zebrafish.

Figure 1

Open in a new tab

(a,b) Zebrafish larvae from nrg2a+/− incrosses were imaged at 120 hpf; lateral view, anterior to the left; scale bars, 0.5 mm. nrg2a−/− larvae can be recognized by their defective jaws (asterisk in b). (c–k) nrg2a mutants lack cardiac trabeculae. Confocal images (mid-sagittal sections) of larval hearts from Tg(myl7:LIFEACT-GFP);nrg2a+/− incrosses at 75 (c–e), 120 (f–h) and 168 hpf (i–k); ventricular outer curvature (dashed boxes) magnified in upper right corners; arrows and asterisks indicate trabeculated and non-trabeculated walls, respectively; scale bars, 50 μm.

Differential Nrg2a expression in the ventricle and atrium

Trabeculae start to form in zebrafish around 60 hpf and become clearly apparent by 72 hpf5,26. To investigate why trabeculation occurs primarily in the ventricle and not the atrium3,30, we examined Nrg2a-mRFP expression at several stages of cardiac development. We used high-resolution confocal microscopy to image live nrg2a+/− animals in the Tg(kdrl:NLS-EGFP) background, in which the endothelial cell nuclei are labelled with GFP36, at 52, 78 and 120 hpf (Fig. 2a–i). Nrg2a-mRFP expression appears in the endocardium at 52 hpf, particularly in the outer curvature of the ventricle (Fig. 2a–c, Supplementary Fig. 2a–c and Fig. 3a–c). Subsequently, Nrg2a-mRFP expression becomes stronger in ventricular and atrial endocardial cells (Fig. 2d–i, Supplementary Fig. 2d–i, Fig. 3d–i), although it is weaker in the atrioventricular (AV) canal (Fig. 2d–i and Supplementary Fig. 4a–c). Accordingly, nrg2a mutants develop functional AV valve leaflets (Supplementary Fig. 4d–i) as was observed in erbb2 mutants5, suggesting that Nrg2a/Erbb2 signalling is not required for AV valve development.

Figure 2. Nrg2a-mRFP expression during embryonic and larval cardiac development.

Figure 2

Open in a new tab

(a–i) Two-dimensional (2D) confocal images (mid-sagittal sections) of zebrafish hearts from Tg(kdrl:NLS-EGFP);nrg2a+/− outcrosses at 52 (a–c), 78 (d–f) and 120 hpf (g–i) showing that Nrg2a-mRFP expression is clearly visible in the ventricular endocardium by 52 hpf, mainly in the outer curvature (a–c), and that it becomes stronger in both ventricular and atrial chambers at 78 and 120 hpf (d–i); however, it is weak in the atrioventricular canal; arrowheads point to the superior valve leaflet (a–i); AV, atrioventricular canal, At, atrium; V, ventricle; scale bars, 50 μm. (j) Cell-based mRFP intensity, measured with the ZEN Imaging Software and plotted as a graph, showing that Nrg2a-mRFP is more highly expressed in the ventricle compared to the AV canal and atrium; dots in this graph represent individual Nrg2a-mRFP expressing endocardial cells. (k) Nrg2a-mRFP positive endocardial cells counted in each chamber at 78 hpf, showing that there are more Nrg2a-mRFP positive endocardial cells in the ventricle than in the atrium at 78 hpf; dots in this graph represent individual hearts; N=5 hearts; values represent means±s.e.m.; **P≤0.01, ***P≤0.001 by Student's t-test.

Figure 3. Reduction of cardiac jelly thickness in developing zebrafish.

Figure 3

Open in a new tab

(a–h) Mid-sagittal confocal sections of late embryonic and early larval zebrafish hearts. Animals from Tg(kdrl:Hsa.HRAS-mCherry);Tg(myl7:EGFP-Hsa.HRAS) incrosses were synchronized at the tail bud stage (10 hpf) and imaged at 48 (a), 54 (b), 60 (c), 72 (d), 80 (e), 96 (f), 120 (g) and 144 hpf (h); endocardial and myocardial membranes are labelled in red and green, respectively; AV, atrioventricular canal, At, atrium; V, ventricle; scale bars, 50 μm. Higher magnification images of outer curvature area of ventricular (white dashed boxes) and atrial (yellow dashed boxes) walls are shown beneath each time point, respectively. The cardiac jelly is initially thicker in the atrium than in the ventricle at 48 hpf (a). It is greatly reduced in the ventricle by 72 hpf, (b–d) and almost fully gone by 96 hpf, while it is still detectable at 120 hpf in the atrium (e–h); arrowheads point to trabecular cardiomyocytes; arrows and asterisks indicate the presence and absence of cardiac jelly, respectively.

Utilizing the Zeiss Efficient Navigation (ZEN) software, we measured the levels of Nrg2a-mRFP expression as well as the number of Nrg2a-mRFP positive endocardial cells at 78 hpf (Fig. 2j,k). These analyses show that ventricular endocardial cells express higher levels of Nrg2a-mRFP than atrial or AV canal endocardial cells (Fig. 2j), and that there are more Nrg2a-mRFP positive endocardial cells in the ventricle as compared to the other cardiac regions (Fig. 2k). We also found that on average (N=5 hearts), 97% and 90% of endocardial cells in the ventricle and atrium were positive for Nrg2a-mRFP expression, respectively (Supplementary Fig. 2j). Thus, ventricular cardiomyocytes might be exposed to a higher level of Nrg2a ligands than atrial ones, although atrial endocardial cells are clearly positive for Nrg2a-mRFP expression.

To examine whether the Nrg2a protein-trap expression in the endocardium accurately recapitulates endogenous nrg2a expression34,35, we carried out in situ hybridizations on 78 hpf hearts. We observed that nrg2a is more highly expressed in the ventricle, compared to the atrium, and that it is mainly restricted to the outer curvature (Supplementary Fig. 2k). This expression pattern is consistent with that of Nrg2a-mRFP.

The cardiac jelly and the onset of trabeculation

The cardiac jelly is known to be pivotal in endo-myocardial interactions required for cardiac morphogenesis4,37, and its reduction during development has previously been reported in chicken and mice4,38. Using high-resolution confocal microscopy, we decided to investigate more closely the dynamics of cardiac jelly reduction around the onset of trabeculation in Tg(kdrl:Hsa.HRAS-mCherry)39;Tg(myl7:EGFP-Hsa.HRAS)40 animals in which endocardial and myocardial membranes are labelled in red and green, respectively. We found that at 48 hpf the cardiac jelly fully separates the endocardial and myocardial layers (Fig. 3a), and that its thickness gradually decreases in both chambers but more clearly in the ventricle (Fig. 3b–h). The timing of this decrease correlates with the appearance of the first trabeculae in the outer curvature of the ventricle by 60 hpf (Fig. 3b,c, arrowhead). By 96 hpf, the cardiac jelly appears to be fully gone in the ventricle while it remains in the atrium for at least the first 6 days of development (Fig. 3d–h).

To investigate whether Nrg2a signalling modulates the thickness of the cardiac jelly, we incrossed nrg2a+/− animals in the Tg(kdrl:Hsa.HRAS-mCherry);Tg(myl7:LIFEACT-GFP) background, and found no obvious differences between wild types and mutants (Supplementary Fig. 5a–h), similar to what we observed in nrg1bns101 mutants and Erbb2 inhibitor-treated animals (Supplementary Fig. 5i–k), suggesting that Nrg/Erbb2 signalling is not involved in this process in zebrafish.

Altogether, these data show that the reduction of the cardiac jelly takes place in the ventricle as trabeculation starts, suggesting that it may determine the onset of this process, possibly by constituting a diffusion barrier for Nrg ligands.

nrg2a overexpression in cardiomyocytes

The current model is that endocardial-derived Nrg ligands traverse the cardiac jelly to activate cardiomyocyte behaviour and proliferation4,12,41,42. To determine whether ectopic overexpression of nrg2a in cardiomyocytes enables them to form trabeculae in nrg2a mutants, we collected embryos from Tg(myl7:LIFEACT-GFP);nrg2a+/− incrosses and injected them with a myl7:nrg2a-p2a-tdTomato plasmid at the one-cell stage (Fig. 4a,b). This approach allows one to identify the nrg2a overexpressing cardiomyocytes by virtue of their expression of tdTomato. Confocal microscopy analysis showed that ectopic overexpression of nrg2a in nrg2a mutant ventricular cardiomyocytes could drive them to form a multilayered wall in most tdTomato positive hearts (13<15) (Fig. 4c–e). To determine whether Nrg2a signals via Erbb2 in cardiomyocytes, we ectopically overexpressed nrg2a in erbb2st61 mutant cardiomyocytes. Our results show that mosaic myocardial nrg2a overexpression did not rescue the trabeculation defects in erbb2 mutants (Fig. 4f–i), indicating that Nrg2a must signal through Erbb2 in cardiomyocytes.

Figure 4. Myocardial nrg2a overexpression induces cardiomyocyte multilayering in nrg2a mutants.

Figure 4

Open in a new tab

(a) Cartoon of myocardial specific nrg2a construct. (b) Schematic representation of Nrg2a protein tagged by tdTomato. Due to the presence of the P2A peptide, cleavage occurs right after protein translation to separate the Nrg2a from the tdTomato fluorescent protein. (c–e) 2D confocal images (mid-sagittal sections) of Tg(myl7:LIFEACT-GFP);nrg2a−/− hearts injected with myocardial specific nrg2a construct (myl7:nrg2a-p2a-tdTomato) at the one-cell stage. Mosaic overexpression of nrg2a in nrg2a−/− cardiomyocytes led to the formation of a multilayered myocardial wall which is outlined by a white dashed box and magnified (c–e); arrows point to nrg2a overexpressing cardiomyocytes. (f–i) Confocal images (mid-sagittal sections) of 120 hpf Tg(myl7:EGFP-Hsa.HRAS) hearts from erbb2+/− incrosses injected with the myl7:nrg2a-p2a-tdTomato construct (h–i). Magnified images of dashed boxes are shown below c–i; arrows point to nrg2a overexpressing cardiomyocytes, arrowheads point to trabeculae and asterisks indicate lack of trabeculae. At, atrium; V, ventricle; scale bars, 50 μm.

We also generated a stable myocardial-specific Nrg2a transgenic line by injecting wild-type embryos with the myl7:nrg2a-p2a-tdTomato plasmid. Although a majority of the injected animals survived to adulthood, some exhibited pericardial edema due to the presence of a much enlarged heart as compared to those of non-injected animals, indicating that mosaic expression of nrg2a in cardiomyocytes leads to cardiomegaly (Supplementary Fig. 6a–d), as expected from data in mouse using a transgene encoding constitutively active Erbb2 (ref. 21) and in zebrafish using nrg1 overexpression22. Overall, these data indicate that ventricular cardiomyocytes are capable to respond to the myocardial overexpression of nrg2a.

Atrial cardiomyocytes can respond to Nrg2a signalling

To further investigate the correlation between cardiac jelly thickness and the onset of trabeculation, we used our newly generated Tg(myl7:nrg2a-p2a-tdTomato) line to evaluate the behaviour of cardiomyocytes in the presence or absence of Nrg2a signalling (Fig. 5a). The cardiac jelly is present for at least the first 6 days of development in the zebrafish atrium, physically separating the endocardial and myocardial walls (Fig. 3), and thus possibly preventing myocardial cells from receiving endocardial-derived Nrg2a molecules. A myocardial specific nrg2a overexpression approach allows one to circumvent the hypothetical barrier function of the cardiac jelly and test whether atrial cardiomyocytes are in fact competent to respond to Nrg signalling. To better visualize cardiomyocyte boundaries, Tg(myl7:nrg2a-p2a-tdTomato) fish were outcrossed to the myocardial specific membrane line Tg(myl7:EGFP-Hsa.HRAS). Compared to Tg(myl7:nrg2a-p2a-tdTomato) negative animals, we observed that nrg2a overexpression in cardiomyocytes pushed them to form a bilayered wall in the ventricle as well as the atrium starting at 46 and 78 hpf, respectively (Fig. 5b–e, Supplementary Fig. 7a,b). Subsequently, constitutively active Nrg2a signalling resulted in cardiomyocyte multilayering in both ventricular and atrial walls (Fig. 5f–i). However, this cardiomyocyte response was blocked by Erbb2 inhibitor treatment starting at 36 hpf (Supplementary Fig. 8a–d). By crossing the Tg(myl7:nrg2a-p2a-tdTomato) line with a Fucci line (Tg(myl7:mVenus-gmnn)) which labels proliferating cardiomyocytes in green43, we observed that ectopic myocardial expression of nrg2a increased the number of proliferating cardiomyocytes (Supplementary Fig. 9a–f). Overall, these data indicate that both ventricular and atrial cardiomyocytes can respond to Nrg/Erbb2 signalling.

Figure 5. Myocardial specific nrg2a overexpression can lead atrial cardiomyocytes to form a multilayered wall.

Figure 5

Open in a new tab

(a) Illustration of cardiomyocyte specific nrg2a overexpression in zebrafish. (b–i) 2D confocal images (mid-sagittal views) of Tg(myl7:EGFP-Hsa.HRAS) and Tg(myl7:EGFP-Hsa.HRAS);Tg(myl7:nrg2a-p2a-tdTomato) hearts at 50 (b,c), 78 (d,e), 120 (f,g) and 144  hpf (h,i); magnified images of dashed boxes are shown below each time point; arrows point to multilayered walls; At, atrium; V, ventricle; scale bars, 50 μm.

Contractility regulates endocardial nrg2a expression

Physical forces generated by contractility have been previously shown to modulate cardiac trabeculation6,26,27. Taking advantage of the nrg2a protein-trap line, we wanted to investigate how cardiac contractility/blood flow regulates nrg2a expression in the zebrafish heart. At 78 hpf, we observed that endocardial Nrg2a-mRFP is strongly expressed in the outer curvature of wild-type ventricles, where trabeculae are present, while it is clearly weaker in other endocardial cells (Fig. 6a–c,j). The levels of Nrg2a-mRFP expression in the heart were strongly reduced in the absence of cardiac contractility in tnnt2a morphants44 (Fig. 6d–f,k) and 2,3-butanedione monoxime (BDM)-treated larvae (Fig. 6g–i,l), indicating that contraction and/or blood flow is required for cardiac expression of Nrg2a-mRFP. To investigate whether there is also a role for cardiac contractility in regulating Erbb2 downstream signalling pathways, we injected Tg(myl7:EGFP-Has.HRAS);Tg(myl7:nrg2a-202-p2a-tdTomato) embryos with tnnt2a morpholinos and imaged them by confocal microscopy at 78 hpf. Notably, we observed that myocardial overexpression of nrg2a resulted in cardiomyocyte multilayering even in the absence of contractility (Fig. 6m,n). However, overexpressing nrg2a throughout the endothelium, including the endocardium, by using a stable Tg(fli1a:nrg2a-p2a-tdtomato) line did not cause a cardiomyocyte multilayering phenotype in tnnt2a morphants (Supplementary Fig. 10). By injecting low amount of myl7:nrg2a-p2a-tdTomato plasmid DNA in tnnt2a morphants, we also observed that mosaic expression of nrg2a recruits both nrg2a expressing and non-expressing cardiomyocytes to form a multilayered wall suggesting that nrg2a affects the behaviour of cardiomyocytes via both autocrine and paracrine signals (Supplementary Fig. 11). Furthermore, it has been recently reported that N-cadherin relocalizes to the basal side of cardiomyocytes during trabeculation45. We observed that nrg2a overexpression in non-contractile cardiomyocytes was sufficient to drive the relocalization of Cdh2-EGFP molecules to their basal side (Fig. 6o–r, Supplementary Movie 1) while they remained mostly localized on the lateral sides of non-nrg2a expressing cardiomyocytes, further indicating that cardiac contraction is not necessary for cardiomyocytes to respond to Nrg2a signalling. Altogether, these results support a model in which contractility/blood flow is required for endocardial expression of nrg2a but not the expression or function of Nrg2a/Erbb2 signalling effectors (Fig. 6s).

Figure 6. Contractility/blood flow is required for endocardial expression of nrg2a but not the ability of cardiomyocytes to respond to nrg2a.

Figure 6

Open in a new tab

(a–i) Maximum intensity z-projections (25–30 z-stacks, mid-sagittal sections) of Tg(kdrl:NLS-EGFP);nrg2a+/− hearts from non-treated (a–c), tnnt2a MO injected (d–f) and BDM treated (g–i) 78 hpf larvae; scale bars, 50 μm. (j–l) Graphs showing cell-based Nrg2a-mRFP intensity in outer curvature (yellow box) and inner curvature (green box) of hearts from non-treated (j), tnnt2a MO (k) and BDM treated (l) 78 hpf larvae; dots represent individual Nrg2a-mRFP expressing endocardial cells. Values represent means±s.e.m.; **P≤0.01, ***P≤0.001, NS (not significant), by Student's t-test. (m,n) 2D confocal images (mid-sagittal sections) of 78 hpf Tg(myl7:EGFP-Hsa.HRAS) (m) or Tg(myl7:EGFP-Hsa.HRAS);Tg(myl7:nrg2a-p2a-tdTomato) hearts (n) showing that myocardial overexpression of nrg2a can induce cardiomyocyte multilayering in tnnt2a morphants; asterisks and arrows indicate single-layered and multilayered ventricular walls, respectively. (o–r) Maximum intensity z-projections of TgBAC(cdh2:cdh2-EGFP) hearts from non-injected (o), injected with tnnt2a MO alone (p) or injected with tnnt2a MO and myl7:nrg2a-p2a-tdTomato plasmid (q,r) 96 hpf larvae. (r) 2D confocal image (sagittal section) of heart shown in q. Magnified images of dashed boxes are shown below (o–r); arrowheads and lozenges indicate presence and absence of Cdh2-EGFP proteins on the basal side of cardiomyocytes, respectively; At: atrium; V, ventricle; scale bars, 50 μm. (s) Schematic diagram of modulation of Nrg2a/Erbb2 signaling by cardiac contractility/blood flow.

Discussion

During trabeculation, cardiomyocytes undergo several morphogenetic changes required for the luminal growth and maintenance of the myocardial wall2,5,6,8,33,43,45. Taking advantage of high-resolution live imaging in the translucent zebrafish larvae, we investigated, using loss- and gain-of-function approaches, why trabeculation takes place primarily in the ventricle and how it is controlled by cardiac contractility/blood flow.

Given the importance of Nrg1 in cardiac trabeculation in mouse11,46,47, we investigated the role of Nrg1 in zebrafish and found that it is not required for trabeculation, a finding consistent with a very recent publication48. Instead we found that Nrg2a is required for trabeculation in zebrafish while loss of Nrg2 does not lead to any obvious cardiac defects in mouse49. Nrg signalling plays many essential roles in vertebrate development and homeostasis5,12,41,50,51, and it will be interesting to investigate ligand selection across evolutionary time. We also observed that Nrg1 and Nrg2a signalling are not required for AV valve formation in zebrafish, consistent with previous observations of erbb2 mutants5. In mouse, Erbb2–Erbb3 heterodimer activity is required for early AV valve formation52,53, and it will be interesting to investigate the role of Erbb3 in zebrafish heart development.

To investigate why trabeculae form only in the ventricle, we used the nrg2amn0237Gt/+ protein-trap line35 to monitor the endogenous expression of nrg2a, and found that it is expressed in the endocardium similar to mouse Nrg1 and Nrg2 (refs 54, 55). Our results also indicate that Nrg2a-mRFP is strongly expressed in endocardial cells lining the outer curvature of the ventricle whereas it is weaker in other endocardial cells suggesting that some ventricular cardiomyocytes might be exposed to higher level of Nrg2a signalling. One caveat here of course is that we examined the expression of a fusion protein which is likely to have very different stability than the endogenous Nrg2a protein.

Prior studies have also revealed the critical role of the cardiac jelly in endo-myocardial interactions16,56,57. Investigating the dynamics of cardiac jelly degradation during early cardiac development, we observed that by the time trabeculae appear, little cardiac jelly is left in the ventricle. By ectopic myocardial overexpression of nrg2a, we found that atrial cardiomyocytes could also respond to Nrg2a/Erbb2 signalling. We speculate that atrial cardiomyocytes receive less Nrg ligands, which are expressed by endocardial cells, due to the thickness of the cardiac jelly in the atrium and consequently do not form a multilayered wall.

When we overexpressed nrg2a in cardiomyocytes using the myl7 promoter, we observed the formation of multiple continuous layers rather than the formation of trabeculae, possibly because of the high levels, even distribution and premature expression of nrg2a. We hypothesize that the expression of lower levels of nrg2a in single cardiomyocytes starting at the right stage would lead them to delaminate and seed trabeculae as observed in wild-type embryos, and new transgenic tools will be required to test this hypothesis. In addition, higher levels of Nrg2a signalling might drive cardiomyocyte proliferation.

Several groups have shown the importance of physical forces in cardiac morphogenesis6,24,27,58,59. It has recently been reported that in zebrafish cardiac contraction is required for cardiac nrg1 expression but not for cardiac erbb2 expression27. Here, we showed that contractility/blood flow is essential for cardiac expression of nrg2a. Interestingly, although nrg1 upregulation by broad expression of a Notch intracellular domain was not sufficient to rescue trabeculation defects in tnnt2a morphants27, we could induce cardiomyocyte multilayering in these animals by myocardial overexpression of nrg2a. Moreover, we also showed that ectopic overexpression of nrg2a in non-contractile cardiomyocytes led to the relocalization of Cdh2-EGFP to their basal side. Overall, these data indicate that contractility/blood flow is indispensable for cardiac expression of nrg2a but not the ability of cardiomyocytes to respond to nrg2a.

We also observed that unlike myocardial specific nrg2a overexpression, endocardial specific nrg2a overexpression did not induce cardiomyocyte multilayering in tnnt2a morphants, likely due to the presence of a substantial gap between the endocardial and myocardial layers. These data further suggest that the thickness of the cardiac jelly may play an important role in determining when trabeculation starts.

In conclusion, our data indicate that trabeculae may initially form only in the ventricle in part because the cardiac jelly in the atrium does not get degraded as fast as it does in the ventricle. Atrial cardiomyocytes exhibit another kind of behaviour as they form the inner pectinate myofibers60,61, and it will be interesting to investigate the underlying signalling pathways. We also found that biomechanical forces work upstream of Nrg2a/Erbb2 signalling to modulate trabeculation in the zebrafish heart by modulating nrg2a expression in the endocardium. It will be important to identify the proteins involved in sensing these forces as well as the transcriptional effectors regulating nrg2a expression.

Methods

Zebrafish husbandry

Embryonic and adult zebrafish were raised and maintained under standard conditions62 and all animal experiments were done according to German Animal Protection Laws approved by the local governmental animal protection committee.

Zebrafish transgenic and mutant lines

In this study, we used the following transgenic and mutant lines: Tg(kdrl:Hsa.HRAS-mCherry)s896 (ref. 39), Tg(myl7:EGFP-Hsa.HRAS)s883 (ref. 40), Tg(myl7:LIFEACT-GFP)s974 (ref. 33), Tg(kdrl:NLS-EGFP)ubs1 (ref. 36), TgBAC(cdh2:cdh2-EGFP,crybb1:ECFP)zf517 (ref. 63) abbreviated as TgBAC(cdh2:cdh2-EGFP), Tg(myl7:nrg2a202-p2a-tdTomato)bns140 abbreviated as Tg(myl7:nrg2a-p2a-tdTomato), Tg(fli1a:nrg2a202-p2a-tdTomato)bns199 abbreviated as Tg(fli1a:nrg2a-p2a-tdTomato) and Tg(myl7:mVenus-gmnn)ncv43Tg (ref. 43) abbreviated as Tg(myl7:mVenus-gmnn), nrg2amn0237Gt (ref. 35), erbb2st61 (ref. 64) and nrg1bns101.

Generation of nrg1 bns101 mutant

nrg1 mutants were generated by TALEN mutagenesis targeting exon 2. The following TALEN arms were constructed and assembled using the Golden Gate method31.

TALEN arm1: NG NG NG NN NN HD NI NN NN NI NI NI HD HD HD NI NN

TALEN arm2: HD NG NG NG NG HD HD NN NG NG HD NG NG NN NG NI HD HD

An amount of 100 pg of total TALEN RNA and 50 pg of GFP RNA (used to monitor injection efficiency) were coinjected into the cell at the one-cell stage.

The nrg1bns101allele was genotyped using high-resolution melt analysis (HRMA) with an Eco Real-Time PCR System (Illumina).

The following HRMA primers were used for PCR reactions:

nrg1-exon2- F1 5′-GAGATTTTGGCAGGAAACCC-3′

nrg1-exon2- R1 5′-CTTTATGCTTTTGGGTTTG-3′.

Mosaic and stable overexpression of nrg2a

To generate a myocardial specific nrg2a overexpressing construct, the nrg2a-202 coding sequence followed by p2a-tdTomato was cloned in a mini tol2 plasmid harbouring a myl7 promoter using the Cold Fusion Cloning Kit (MC101A-1-SB). For mosaic expression of nrg2a, 15 pg of myl7:nrg2a-202-p2a-tdTomato plasmid DNA was coinjected with 10 ng of Tol2 RNA into the cell at the one-cell stage.

To establish the Tg(myl7:nrg2a202-p2a-Tomato)bns140 line, injected larvae (F0) were screened for myocardial tdTomato fluorescence and raised to adulthood. After identifying founders, F1 embryos positive for fluorescence in their cardiomyocyte were raised to adulthood.

Morpholino injections

To stop cardiac contraction, 0.5 ng of a tnnt2a morpholino (5′-CATGTTTGCTCTGATCTGACACGCA-3′) was injected into one-cell stage embryos44.

Chemical treatments

To prevent contractility for 6 h (short term treatment), 72 hpf zebrafish larvae were exposed to 20 mM BDM65. To block Erbb2 signalling, dechorionated embryos were treated with 10 μM of the Erbb2 inhibitor PD 168393 (Calbiochem) from 36 to 96 hpf.

In vivo confocal imaging and data processing

Zebrafish embryos and larvae were mounted and anesthetized in 1.5% low-melt agarose (Sigma) containing 0.2 per cent tricaine on glass-bottom dishes. Before the gel solidified, the samples were manually oriented towards the microscope lens to enhance optical access to the heart. Images were captured with a Zeiss LSM780 or a spinning disk (CSU-X1 Yokogawa) confocal microscope using × 40 (1.1 NA) and × 40 (1.15 NA) water immersion lenses, respectively. The confocal data were then processed with the ZEN 2012 software (black edition). Nrg2a-mRFP intensity per cell was measured with the ZEN 2012 software (blue edition).

Whole mount in situ hybridization

To perform whole mount in situ hybridization66, 78 hpf zebrafish larvae were fixed in 4% paraformaldehyde overnight at 4 °C and subsequently dehydrated in 100% methanol at −20 °C. Larvae were rehydrated with 1 × PBS and permeabilized by digestion with proteinase K (10 μg ml−1) at room temperature for 30 min. After washing with 1 × PBT (1 × phosphate buffered saline (PBS), 0.1% Tween 20 (vol/vol)), larvae were hybridized with 200 ng of nrg2a antisense DIG-labelled RNA probe overnight at 70 °C. The hybridized probes were then detected with alkaline phosphatase-conjugated anti-Digoxigenin antibody (Roche, dilution 1:1,000) for three hours at room temperature and the signal was visualized with BM purple (Roche).

The probe for nrg2a was amplified from 78 hpf heart cDNA using nrg2a forward 5′-TCTGTGTGGCTCTTTTGGTG-3′ and reverse 5′-GTGCGTCAGTCTGGTCTGTG-3′ primers. The PCR fragment was subcloned into pGEM-T.

Statistical analysis

Data were processed with the Prism5 software. Values are presented as mean±s.e. P values (*P≤0.05, **P≤0.01, ***P≤0.001) were calculated using Student's t-test.

Data availability

The authors declare that all data supporting the findings of this study are available within the article and its Supplementary Information files or from the corresponding author upon reasonable request.

Additional information

How to cite this article: Rasouli, S. J. et al. Regulation of cardiomyocyte behavior in zebrafish trabeculation by Neuregulin 2a signaling. Nat. Commun. 8, 15281 doi: 10.1038/ncomms15281 (2017).

Publisher's note: Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Supplementary Material

Supplementary Information

Supplementary Figures, Supplementary References.

ncomms15281-s1.pdf (4MB, pdf)
Supplementary Movie 1

Overexpression of nrg2a in non-contractile cardiomyocytes induces basal relocalization of Cdh2-EGFP. Movie reconstructed from 9 confocal sagittal sections (Z-stacks from apical to basal) of the Tg(myl7:EGFP-Hsa.HRAS) heart shown in Figure 6q showing the relocalization of Cdh2-EGFP proteins to the basal side of cardiomyocytes; arrows point to cardiomyocytes with basal Cdh2-EGFP molecules; scale bar, 50 μm.

Download video file (638.7KB, mov)

Acknowledgments

We would like to acknowledge Matthias Hammerschmidt for pointing out the late larval lethality in nrg2a mutants and thank him and Stephen Ekker, Stephanie Westcot and Julia Hatzold for generously providing nrg2amn0237Gtanimals prior to publication. We thank Radhan Ramadass for confocal imaging and technical support, Mohamed El-Brolosy, Vanesa Jimenez-Amilburu, Jason Lai, Anoop Cherian, Ryuichi Fukuda and Parisa Ghanbari for sharing reagents and helpful discussions, Sabine Fischer and Sharon Meaney-Gardian for excellent assistance, as well as Jean-Francois Matuszek, Sophie Mucenieks, Rita Retzloff and Martin Laszczyk for zebrafish care. This work was supported by the Max Planck Society and the Leducq Foundation.

Footnotes

The authors declare no competing financial interests.

Author contributions S.J.R. and D.Y.R.S. designed the experiments. S.J.R. performed all the experiments. S.J.R. and D.Y.R.S. analysed data, wrote and revised the manuscript. D.Y.R.S. supervised the project.

References

  1. Staudt D. & Stainier D. Uncovering the molecular and cellular mechanisms of heart development using the zebrafish. Annu. Rev. Genet. 46, 397–418 (2012). [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Sedmera D. & Thomas P. S. Trabeculation in the embryonic heart. Bioessays 18, 607–607 (1996). [DOI] [PubMed] [Google Scholar]
  3. Sedmera D., Pexieder T., Vuillemin M., Thompson R. P. & Anderson R. H. Developmental patterning of the myocardium. Anat. Rec. 258, 319–337 (2000). [DOI] [PubMed] [Google Scholar]
  4. Stankunas K. et al. Endocardial Brg1 represses ADAMTS1 to maintain the microenvironment for myocardial morphogenesis. Dev. Cell 14, 298–311 (2008). [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Liu J. et al. A dual role for ErbB2 signaling in cardiac trabeculation. Development 137, 3867–3875 (2010). [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Staudt D. W. et al. High-resolution imaging of cardiomyocyte behavior reveals two distinct steps in ventricular trabeculation. Development 141, 585–593 (2014). [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gassmann M., Casagranda F., Orioli D. & Simon H. Aberrant neural and cardiac development in mice lacking the ErbB4 neuregulin receptor. Nature 378, 390 (1995). [DOI] [PubMed] [Google Scholar]
  8. Lee K.-F. et al. Requirement for neuregulin receptor erbB2 in neural and cardiac development. Nature 378, 394–398 (1995). [DOI] [PubMed] [Google Scholar]
  9. Jenni R., Rojas J. & Oechslin E. Isolated noncompaction of the myocardium. N. Engl. J. Med. 340, 966–967 (1999). [DOI] [PubMed] [Google Scholar]
  10. Morris J. K. et al. Rescue of the cardiac defect in ErbB2 mutant mice reveals essential roles of ErbB2 in peripheral nervous system development. Neuron 23, 273–283 (1999). [DOI] [PubMed] [Google Scholar]
  11. Crone S. A. et al. ErbB2 is essential in the prevention of dilated cardiomyopathy. Nat. Med. 8, 459–465 (2002). [DOI] [PubMed] [Google Scholar]
  12. Lai D. et al. Neuregulin 1 sustains the gene regulatory network in both trabecular and nontrabecular myocardium. Circ. Res. 107, 715–727 (2010). [DOI] [PubMed] [Google Scholar]
  13. Brutsaert D. L. et al. The cardiac endothelium: functional morphology, development, and physiology. Prog. Cardiovasc. Dis. 39, 239–262 (1996). [DOI] [PubMed] [Google Scholar]
  14. Tian Y. & Morrisey E. E. Importance of myocyte-nonmyocyte interactions in cardiac development and disease. Circ. Res. 110, 1023–1034 (2012). [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Bowers S. L. & Baudino T. A. Laying the groundwork for growth: cell–cell and cell–ECM interactions in cardiovascular development. Birth Defects Res. C Embryo Today 90, 1–7 (2010). [DOI] [PubMed] [Google Scholar]
  16. Ramasubramanian A. et al. On the role of intrinsic and extrinsic forces in early cardiac S-looping. Dev. Dyn. 242, 801–816 (2013). [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Suri C. et al. Requisite role of angiopoietin-1, a ligand for the TIE2 receptor, during embryonic angiogenesis. Cell 87, 1171–1180 (1996). [DOI] [PubMed] [Google Scholar]
  18. Meyer D. & Birchmeier C. Multiple essential functions of neuregulin in development. Nature 378, 386–390 (1995). [DOI] [PubMed] [Google Scholar]
  19. Bersell K., Arab S., Haring B. & Kühn B. Neuregulin1/ErbB4 signaling induces cardiomyocyte proliferation and repair of heart injury. Cell 138, 257–270 (2009). [DOI] [PubMed] [Google Scholar]
  20. Polizzotti B. D. et al. Neuregulin stimulation of cardiomyocyte regeneration in mice and human myocardium reveals a therapeutic window. Sci. Transl. Med. 7, 281ra245–281ra245 (2015). [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. D'Uva G. et al. ERBB2 triggers mammalian heart regeneration by promoting cardiomyocyte dedifferentiation and proliferation. Nat. Cell Biol. 17, 627–638 (2015). [DOI] [PubMed] [Google Scholar]
  22. Gemberling M., Karra R., Dickson A. L. & Poss K. D. Nrg1 is an injury-induced cardiomyocyte mitogen for the endogenous heart regeneration program in zebrafish. Elife 4, e05871 (2015). [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Collins M. M. & Stainier D. Y. Chapter twenty-five-organ function as a modulator of organ formation: lessons from zebrafish. Curr. Top. Dev. Biol. 117, 417–433 (2016). [DOI] [PubMed] [Google Scholar]
  24. Bartman T. et al. Early myocardial function affects endocardial cushion development in zebrafish. PLoS Biol. 2, e129 (2004). [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Auman H. J. et al. Functional modulation of cardiac form through regionally confined cell shape changes. PLoS Biol. 5, e53 (2007). [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Peshkovsky C., Totong R. & Yelon D. Dependence of cardiac trabeculation on neuregulin signaling and blood flow in zebrafish. Dev. Dyn. 240, 446–456 (2011). [DOI] [PubMed] [Google Scholar]
  27. Samsa L. A. et al. Cardiac contraction activates endocardial Notch signaling to modulate chamber maturation in zebrafish. Development 142, 4080–4091 (2015). [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Sedmera D., Pexieder T., Rychterova V., Hu N. & Clark E. B. Remodeling of chick embryonic ventricular myoarchitecture under experimentally changed loading conditions. Anat. Rec. 254, 238–252 (1999). [DOI] [PubMed] [Google Scholar]
  29. Berdougo E., Coleman H., Lee D. H., Stainier D. Y. & Yelon D. Mutation of weak atrium/atrial myosin heavy chain disrupts atrial function and influences ventricular morphogenesis in zebrafish. Development 130, 6121–6129 (2003). [DOI] [PubMed] [Google Scholar]
  30. Moorman A., Webb S., Brown N. A., Lamers W. & Anderson R. H. Development of the heart:(1) formation of the cardiac chambers and arterial trunks. Heart 89, 806–814 (2003). [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Cermak T. et al. Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting. Nucleic Acids Res. 39, 4942–4948 (2011). [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Honjo Y., Kniss J. & Eisen J. S. Neuregulin-mediated ErbB3 signaling is required for formation of zebrafish dorsal root ganglion neurons. Development 135, 2615–2625 (2008). [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Reischauer S., Arnaout R., Ramadass R. & Stainier D. Y. Actin binding GFP allows 4D in vivo imaging of myofilament dynamics in the zebrafish heart and the identification of Erbb2 signaling as a remodeling factor of myofibril architecture. Circ. Res. 115, 845–856 (2014). [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Clark K. J. et al. In vivo protein trapping produces a functional expression codex of the vertebrate proteome. Nat. Methods 8, 506–512 (2011). [DOI] [PMC free article] [PubMed] [Google Scholar]
  35. Westcot S. E. et al. Protein-Trap insertional mutagenesis uncovers new genes involved in zebrafish skin development, including a Neuregulin 2a-based ErbB signaling pathway required during median fin fold morphogenesis. PLoS ONE 10, e0130688 (2015). [DOI] [PMC free article] [PubMed] [Google Scholar]
  36. Blum Y. et al. Complex cell rearrangements during intersegmental vessel sprouting and vessel fusion in the zebrafish embryo. Dev. Biol. 316, 312–322 (2008). [DOI] [PubMed] [Google Scholar]
  37. Lockhart M., Wirrig E., Phelps A. & Wessels A. Extracellular matrix and heart development. Birth Defects Res. A Clin. Mol. Teratol. 91, 535–550 (2011). [DOI] [PMC free article] [PubMed] [Google Scholar]
  38. Manasek F. J. Histogenesis of the embryonic myocardium. Am. J. Cardiol. 25, 149–168 (1970). [DOI] [PubMed] [Google Scholar]
  39. Chi N. C. et al. Foxn4 directly regulates tbx2b expression and atrioventricular canal formation. Genes Dev. 22, 734–739 (2008). [DOI] [PMC free article] [PubMed] [Google Scholar]
  40. D'Amico L., Scott I. C., Jungblut B. & Stainier D. Y. A mutation in zebrafish hmgcr1b reveals a role for isoprenoids in vertebrate heart-tube formation. Curr. Biol. 17, 252–259 (2007). [DOI] [PubMed] [Google Scholar]
  41. Falls D. L. Neuregulins: functions, forms, and signaling strategies. Exp. Cell Res. 284, 14–30 (2003). [DOI] [PubMed] [Google Scholar]
  42. Odiete O., Hill M. F. & Sawyer D. B. Neuregulin in cardiovascular development and disease. Circ. Res. 111, 1376–1385 (2012). [DOI] [PMC free article] [PubMed] [Google Scholar]
  43. Jiménez-Amilburu V. et al. In vivo visualization of cardiomyocyte apicobasal polarity reveals epithelial to mesenchymal-like transition during cardiac trabeculation. Cell Rep. 17, 2687–2699 (2016). [DOI] [PubMed] [Google Scholar]
  44. Sehnert A. J. et al. Cardiac troponin T is essential in sarcomere assembly and cardiac contractility. Nat. Genet. 31, 106–110 (2002). [DOI] [PubMed] [Google Scholar]
  45. Cherian A. V., Fukuda R., Augustine S. M., Maischein H.-M. & Stainier D. Y. N-cadherin relocalization during cardiac trabeculation. Proc. Natl Acad. Sci. USA 113, 7569–7574 (2016). [DOI] [PMC free article] [PubMed] [Google Scholar]
  46. Kramer R. et al. Neuregulins with an Ig-like domain are essential for mouse myocardial and neuronal development. Proc. Natl Acad. Sci. USA 93, 4833–4838 (1996). [DOI] [PMC free article] [PubMed] [Google Scholar]
  47. Wadugu B. & Kühn B. The role of neuregulin/ErbB2/ErbB4 signaling in the heart with special focus on effects on cardiomyocyte proliferation. Am. J. Physiol. Heart Circ. Physiol. 302, H2139–H2147 (2012). [DOI] [PMC free article] [PubMed] [Google Scholar]
  48. Samsa L. A., Ito C. E., Brown D. R., Qian L. & Liu J. IgG-containing isoforms of Neuregulin-1 are dispensable for cardiac trabeculation in zebrafish. PLoS ONE 11, e0166734 (2016). [DOI] [PMC free article] [PubMed] [Google Scholar]
  49. Britto J. M. et al. Generation and characterization of neuregulin-2-deficient mice. Mol. Cell. Biol. 24, 8221–8226 (2004). [DOI] [PMC free article] [PubMed] [Google Scholar]
  50. Horie T. et al. Acute doxorubicin cardiotoxicity is associated with miR-146a-induced inhibition of the neuregulin-ErbB pathway. Cardiovasc. Res. 87, 656–664 (2010). [DOI] [PMC free article] [PubMed] [Google Scholar]
  51. Cantó C. et al. Neuregulins increase mitochondrial oxidative capacity and insulin sensitivity in skeletal muscle cells. Diabetes 56, 2185–2193 (2007). [DOI] [PubMed] [Google Scholar]
  52. Camenisch T. D., Schroeder J. A., Bradley J., Klewer S. E. & McDonald J. A. Heart-valve mesenchyme formation is dependent on hyaluronan-augmented activation of ErbB2–ErbB3 receptors. Nat. Med. 8, 850–855 (2002). [DOI] [PubMed] [Google Scholar]
  53. Armstrong E. J. & Bischoff J. Heart valve development endothelial cell signaling and differentiation. Circ. Res. 95, 459–470 (2004). [DOI] [PMC free article] [PubMed] [Google Scholar]
  54. Gitler A. D., Lu M. M., Jiang Y. Q., Epstein J. A. & Gruber P. J. Molecular markers of cardiac endocardial cushion development. Dev. Dyn. 228, 643–650 (2003). [DOI] [PubMed] [Google Scholar]
  55. Carraway K. L. III et al. Neuregulin-2, a new ligand of ErbB3/ErbB4-receptor tyrosine kinases. Nature 387, 512–516 (1997). [DOI] [PubMed] [Google Scholar]
  56. Nakamura A. & Manasek F. J. An experimental study of the relation of cardiac jelly to the shape of the early chick embryonic heart. Development 65, 235–256 (1981). [PubMed] [Google Scholar]
  57. Mellman K., Huisken J., Dinsmore C., Hoppe C. & Stainier D. Y. Fibrillin-2b regulates endocardial morphogenesis in zebrafish. Dev. Biol. 372, 111–119 (2012). [DOI] [PubMed] [Google Scholar]
  58. Hove J. R. et al. Intracardiac fluid forces are an essential epigenetic factor for embryonic cardiogenesis. Nature 421, 172–177 (2003). [DOI] [PubMed] [Google Scholar]
  59. Dietrich A.-C., Lombardo V. A., Veerkamp J., Priller F. & Abdelilah-Seyfried S. Blood flow and Bmp signaling control endocardial chamber morphogenesis. Dev. Cell 30, 367–377 (2014). [DOI] [PubMed] [Google Scholar]
  60. Foglia M. J., Cao J., Tornini V. A. & Poss K. D. Multicolor mapping of the cardiomyocyte proliferation dynamics that construct the atrium. Development 143, 1688–1696 (2016). [DOI] [PMC free article] [PubMed] [Google Scholar]
  61. Singleman C. & Holtzman N. G. Analysis of postembryonic heart development and maturation in the zebrafish, Danio rerio. Dev. Dyn. 241, 1993–2004 (2012). [DOI] [PMC free article] [PubMed] [Google Scholar]
  62. Westerfield M. in The Zebrafish Book (University of Oregon Press, 2000).
  63. Revenu C. et al. Quantitative cell polarity imaging defines leader-to-follower transitions during collective migration and the key role of microtubule-dependent adherens junction formation. Development 141, 1282–1291 (2014). [DOI] [PubMed] [Google Scholar]
  64. Lyons D. A. et al. erbb3 and erbb2 are essential for schwann cell migration and myelination in zebrafish. Curr. Biol. 15, 513–524 (2005). [DOI] [PubMed] [Google Scholar]
  65. Higuchi H. & Takemori S. Butanedione monoxime suppresses contraction and ATPase activity of rabbit skeletal muscle. J. Biochem. 105, 638–643 (1989). [DOI] [PubMed] [Google Scholar]
  66. Thisse C. & Thisse B. High-resolution in situ hybridization to whole-mount zebrafish embryos. Nat. Protoc. 3, 59–69 (2008). [DOI] [PubMed] [Google Scholar]

Associated Data

This section collects any data citations, data availability statements, or supplementary materials included in this article.

Supplementary Materials

Supplementary Information

Supplementary Figures, Supplementary References.

ncomms15281-s1.pdf (4MB, pdf)
Supplementary Movie 1

Overexpression of nrg2a in non-contractile cardiomyocytes induces basal relocalization of Cdh2-EGFP. Movie reconstructed from 9 confocal sagittal sections (Z-stacks from apical to basal) of the Tg(myl7:EGFP-Hsa.HRAS) heart shown in Figure 6q showing the relocalization of Cdh2-EGFP proteins to the basal side of cardiomyocytes; arrows point to cardiomyocytes with basal Cdh2-EGFP molecules; scale bar, 50 μm.

Download video file (638.7KB, mov)

Data Availability Statement

The authors declare that all data supporting the findings of this study are available within the article and its Supplementary Information files or from the corresponding author upon reasonable request.

Articles from Nature Communications are provided here courtesy of Nature Publishing Group

RESOURCES