Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1990 Oct;87(20):7871–7874. doi: 10.1073/pnas.87.20.7871

Interleukin 1 induces beta-endorphin secretion via Fos and Jun in AtT-20 pituitary cells.

M O Făgărăsan 1, F Aiello 1, K Muegge 1, S Durum 1, J Axelrod 1
PMCID: PMC54852  PMID: 1978316

Abstract

Previous work had shown that interleukin 1 (IL-1), after a long period of treatment, stimulates beta-endorphin release and potentiates the effects of secretagogues in AtT-20 cells, a mouse anterior pituitary cell line. Treatment of AtT-20 cells with IL-1 induced a transient and early stimulation of mRNA expression by both immediate-early protooncogenes Fos and Jun (mouse c-fos and c-jun). The effect appeared within 30 min, and returned to basal levels after 2 hr. Desensitization of protein kinase C by phorbol ester pretreatment had no effect on the ability of IL-1 to induce Fos and Jun mRNA expression. Somatostatin, an inhibitor of cAMP and beta-endorphin secretion, did not reduce the IL-1 effect on Fos and Jun mRNA expression. Addition to AtT-20 cells of antisense oligonucleotides to Fos and Jun abolished the secretion induced by IL-1. These results indicate that immediate-early signals Fos and Jun are involved in IL-1-induced beta-endorphin secretion in AtT-20 cells.

Full text

PDF
7872

Images in this article

7872Image
on p.7872
7873Image
on p.7873
7873Image
on p.7873

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abou-Samra A. B., Catt K. J., Aguilera G. Involvement of protein kinase C in the regulation of adrenocorticotropin release from rat anterior pituitary cells. Endocrinology. 1986 Jan;118(1):212–217. doi: 10.1210/endo-118-1-212. [DOI] [PubMed] [Google Scholar]
  2. Axelrod J., Reisine T. D. Stress hormones: their interaction and regulation. Science. 1984 May 4;224(4648):452–459. doi: 10.1126/science.6143403. [DOI] [PubMed] [Google Scholar]
  3. Bomsztyk K., Sims J. E., Stanton T. H., Slack J., McMahan C. J., Valentine M. A., Dower S. K. Evidence for different interleukin 1 receptors in murine B- and T-cell lines. Proc Natl Acad Sci U S A. 1989 Oct;86(20):8034–8038. doi: 10.1073/pnas.86.20.8034. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Chirgwin J. M., Przybyla A. E., MacDonald R. J., Rutter W. J. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry. 1979 Nov 27;18(24):5294–5299. doi: 10.1021/bi00591a005. [DOI] [PubMed] [Google Scholar]
  5. Curran T., Franza B. R., Jr Fos and Jun: the AP-1 connection. Cell. 1988 Nov 4;55(3):395–397. doi: 10.1016/0092-8674(88)90024-4. [DOI] [PubMed] [Google Scholar]
  6. Dash P., Lotan I., Knapp M., Kandel E. R., Goelet P. Selective elimination of mRNAs in vivo: complementary oligodeoxynucleotides promote RNA degradation by an RNase H-like activity. Proc Natl Acad Sci U S A. 1987 Nov;84(22):7896–7900. doi: 10.1073/pnas.84.22.7896. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Farrar W. L., Ferris D. K., Harel-Bellan A. The molecular basis of immune cytokine action. Crit Rev Ther Drug Carrier Syst. 1989;5(4):229–261. [PubMed] [Google Scholar]
  8. Făgăraşan M. O., Eskay R., Axelrod J. Interleukin 1 potentiates the secretion of beta-endorphin induced by secretagogues in a mouse pituitary cell line (AtT-20). Proc Natl Acad Sci U S A. 1989 Mar;86(6):2070–2073. doi: 10.1073/pnas.86.6.2070. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Făgărăan M. O., Axelrod J. Interleukin-1 amplifies the action of pituitary secretagogues via protein kinases. Int J Neurosci. 1990 Apr;51(3-4):311–313. doi: 10.3109/00207459008999729. [DOI] [PubMed] [Google Scholar]
  10. Făgărăan M. O., Bishop J. F., Rinaudo M. S., Axelrod J. Interleukin 1 induces early protein phosphorylation and requires only a short exposure for late induced secretion of beta-endorphin in a mouse pituitary cell line. Proc Natl Acad Sci U S A. 1990 Apr;87(7):2555–2559. doi: 10.1073/pnas.87.7.2555. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Heisler S., Reisine T. D., Hook V. Y., Axelrod J. Somatostatin inhibits multireceptor stimulation of cyclic AMP formation and corticotropin secretion in mouse pituitary tumor cells. Proc Natl Acad Sci U S A. 1982 Nov;79(21):6502–6506. doi: 10.1073/pnas.79.21.6502. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kawasaki E. S. Quantitative hybridization-arrest of mRNA in Xenopus oocytes using single-stranded complementary DNA or oligonucleotide probes. Nucleic Acids Res. 1985 Jul 11;13(13):4991–5004. doi: 10.1093/nar/13.13.4991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Landschulz W. H., Johnson P. F., McKnight S. L. The leucine zipper: a hypothetical structure common to a new class of DNA binding proteins. Science. 1988 Jun 24;240(4860):1759–1764. doi: 10.1126/science.3289117. [DOI] [PubMed] [Google Scholar]
  14. Loke S. L., Stein C. A., Zhang X. H., Mori K., Nakanishi M., Subasinghe C., Cohen J. S., Neckers L. M. Characterization of oligonucleotide transport into living cells. Proc Natl Acad Sci U S A. 1989 May;86(10):3474–3478. doi: 10.1073/pnas.86.10.3474. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Muegge K., Durum S. K. From cell code to gene code: cytokines and transcription factors. New Biol. 1989 Dec;1(3):239–246. [PubMed] [Google Scholar]
  16. Muegge K., Williams T. M., Kant J., Karin M., Chiu R., Schmidt A., Siebenlist U., Young H. A., Durum S. K. Interleukin-1 costimulatory activity on the interleukin-2 promoter via AP-1. Science. 1989 Oct 13;246(4927):249–251. doi: 10.1126/science.2799385. [DOI] [PubMed] [Google Scholar]
  17. Phillips M., Tashjian A. H., Jr Characterization of an early inhibitory effect of glucocorticoids on stimulated adrenocorticotropin and endorphin release from a clonal strain of mouse pituitary cells. Endocrinology. 1982 Mar;110(3):892–900. doi: 10.1210/endo-110-3-892. [DOI] [PubMed] [Google Scholar]
  18. Ryder K., Nathans D. Induction of protooncogene c-jun by serum growth factors. Proc Natl Acad Sci U S A. 1988 Nov;85(22):8464–8467. doi: 10.1073/pnas.85.22.8464. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Van Beveren C., van Straaten F., Curran T., Müller R., Verma I. M. Analysis of FBJ-MuSV provirus and c-fos (mouse) gene reveals that viral and cellular fos gene products have different carboxy termini. Cell. 1983 Apr;32(4):1241–1255. doi: 10.1016/0092-8674(83)90306-9. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES