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. 1990 Nov;87(21):8462–8466. doi: 10.1073/pnas.87.21.8462

Evidence for positive and negative regulation of the Hox-3.1 gene.

C J Bieberich 1, M F Utset 1, A Awgulewitsch 1, F H Ruddle 1
PMCID: PMC54976  PMID: 1978325

Abstract

The region-specific patterns of expression of mouse homeobox genes are considered important for establishing the embryonic body plan. A 5-kilobase (kb) DNA fragment from the Hox-3.1 locus that is sufficient to confer region-specific expression to a beta-galactosidase reporter gene in transgenic mouse embryos has been defined. The observed reporter gene expression pattern closely parallels endogenous Hox-3.1 expression in 8- to 9.5-day postcoitum (p.c.) embryos. At 10.5 days p.c. and later, the pattern of beta-galactosidase activity diverges from the Hox-3.1 pattern, and an inappropriately high level of reporter gene expression is observed in posterior spinal ganglia. Inclusion of an additional 2 kb of upstream sequences is sufficient to suppress this aberrant expression in the developing spinal ganglia. Together, these results show that the control of early Hox-3.1 expression is complex, involving at least one positively acting and one negatively acting element.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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