Skip to main content
PMC home page
Plant Physiology logoLink to Plant Physiology
. 1966 Sep;41(7):1167–1172. doi: 10.1104/pp.41.7.1167

Protein Synthesis in Imbibed Seeds III. Kinetics of Amino Acid Incorporation Ribosome Activation, and Polysome Formation

Abraham Marcus 1,2, John Feeley 1,2, Tzvi Volcani 1,2
PMCID: PMC550493  PMID: 16656380

Abstract

The kinetics of development of protein-synthesizing capacity in the imbibing wheat embryo, were studied both in vivo and in vitro. During the first 30 minutes of imbibition protein-synthesizing capacity rises rapidly, lagging about 10 minutes behind water uptake. This rise in synthesizing capacity is accompanied by an increase in polysome content. As imbibition continues, both protein-synthesizing capacity and polysome content increase. With embryos from aged seed, the rate of protein synthesis is initially limited by another, presumably nonribosomal, reaction.

Full text

PDF
1169

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. HENSHAW E. C., BOJARSKI T. B., HIATT H. H. PROTEIN SYNTHESIS BY FREE AND BOUND RAT LIVER RIBOSOMES IN VIVO AND IN VITRO. J Mol Biol. 1963 Aug;7:122–129. doi: 10.1016/s0022-2836(63)80041-8. [DOI] [PubMed] [Google Scholar]
  2. Hendler R. W. Lipoamino acids as precursors in the synthesis of authentic proteins by homogenates of hen oviduct. Proc Natl Acad Sci U S A. 1965 Oct;54(4):1233–1240. doi: 10.1073/pnas.54.4.1233. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. MARCUS A., FEELEY J. PROTEIN SYNTHESIS IN IMBIBED SEEDS. II. POLYSOME FORMATION DURING IMBIBITION. J Biol Chem. 1965 Apr;240:1675–1680. [PubMed] [Google Scholar]
  4. Marcus A., Feeley J. ACTIVATION OF PROTEIN SYNTHESIS IN THE IMBIBITION PHASE OF SEED GERMINATION. Proc Natl Acad Sci U S A. 1964 Jun;51(6):1075–1079. doi: 10.1073/pnas.51.6.1075. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Marrè E., Cocucci S., Sturani E. On the Development of the Ribosomal System in the Endosperm of Germinating Castor Bean Seeds. Plant Physiol. 1965 Nov;40(6):1162–1170. doi: 10.1104/pp.40.6.1162. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. STAFFORD D. W., SOFER W. H., IVERSON R. M. DEMONSTRATION OF POLYRIBOSOMES AFTER FERTILIZATION OF THE SEA URCHIN EGG. Proc Natl Acad Sci U S A. 1964 Aug;52:313–316. doi: 10.1073/pnas.52.2.313. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Warner J. R., Rich A., Hall C. E. Electron Microscope Studies of Ribosomal Clusters Synthesizing Hemoglobin. Science. 1962 Dec 28;138(3548):1399–1403. doi: 10.1126/science.138.3548.1399. [DOI] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES