Skip to main content
PMC home page
Plant Physiology logoLink to Plant Physiology
. 1966 Sep;41(7):1213–1217. doi: 10.1104/pp.41.7.1213

Light Dependent Increase of Triosephosphate Dehydrogenase in Pea Leaves 1

B J Ortwerth 1,2, O J Koeppe 1
PMCID: PMC550500  PMID: 5954871

Abstract

Data from 3 lines of investigation were presented indicating that chlorophyll is not necessary for the increase in the triphosphopyridine nucleotide-requiring triosephosphate dehydrogenase accompanying the illumination of etiolated pea plants. These include A) the kinetics of the development of chlorophyll and enzyme activity, B) the presence of enzyme activity in leaves grown in the dark on normal plants and C) the high specific enzyme activity in leaves of a chlorophyll-less mutant.

It was also shown that the light-initiated increase of enzyme activity continues for several days after removal from the light and that illumination with far-red light before the dark period inhibited, but did not abolish, this increase. The ability of green plants to continue to produce the enzyme in the dark was eventually lost with time, for after 7 days in the dark a stimulation in leaf protein formation was not accompanied by an increase in enzyme activity.

Full text

PDF
1217

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arnon D. I. COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS. Plant Physiol. 1949 Jan;24(1):1–15. doi: 10.1104/pp.24.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Fuller R. C., Gibbs M. Intracellular and Phylogenetic Distribution of Ribulose 1,5-Diphosphate Carboxylase and D-Glyceraldehyde-3-Phosphate Dehydrogenases. Plant Physiol. 1959 May;34(3):324–329. doi: 10.1104/pp.34.3.324. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. HAGEMAN R. H., ARNON D. I. Changes in glyceraldehyde phosphate dehydrogenase during the life cycle of a green plant. Arch Biochem Biophys. 1955 Aug;57(2):421–436. doi: 10.1016/0003-9861(55)90304-0. [DOI] [PubMed] [Google Scholar]
  4. Heber U., Pon N. G., Heber M. Localization of Carboxydismutase & Triosephosphate Dehydrogenases in Chloroplasts. Plant Physiol. 1963 May;38(3):355–360. doi: 10.1104/pp.38.3.355. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. JACOB F., MONOD J. Genetic regulatory mechanisms in the synthesis of proteins. J Mol Biol. 1961 Jun;3:318–356. doi: 10.1016/s0022-2836(61)80072-7. [DOI] [PubMed] [Google Scholar]
  6. KEISTER D. L., JAGENDORF A. T., SAN PIETRO A. Development of bean-leaf transhydrogenase in etiolated leaves. Biochim Biophys Acta. 1962 Aug 13;62:332–337. doi: 10.1016/0006-3002(62)90047-1. [DOI] [PubMed] [Google Scholar]
  7. KOSKI V. M., FRENCH C. S., SMITH J. H. C. The action spectrum for the transformation of protochlorophyll to chlorophyll a in normal and albino corn seedlings. Arch Biochem Biophys. 1951 Mar;31(1):1–17. doi: 10.1016/0003-9861(51)90178-6. [DOI] [PubMed] [Google Scholar]
  8. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  9. MEGO J. L., JAGENDORF A. T. Effect of light on growth of Black Valentine bean plastids. Biochim Biophys Acta. 1961 Oct 28;53:237–254. doi: 10.1016/0006-3002(61)90437-1. [DOI] [PubMed] [Google Scholar]
  10. Marcus A. Photocontrol of Formation of Red Kidney Bean Leaf Triphosphopyridine Nucleotide Linked Triosephosphate Dehydrogenase. Plant Physiol. 1960 Jan;35(1):126–128. doi: 10.1104/pp.35.1.126. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Margulies M. M. Relationship Between Red Light Mediated Glyceraldehyde-3-Phosphate Dehydrogenase Formation and Light Dependent Development of Photosynthesis. Plant Physiol. 1965 Jan;40(1):57–61. doi: 10.1104/pp.40.1.57. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. OGSTON A. G., PHELPS C. F. Exclusion of inulin from solutions of hyaluronic acid. Nature. 1960 Sep 17;187:1024–1024. doi: 10.1038/1871024a0. [DOI] [PubMed] [Google Scholar]
  13. Price L., Klein W. H. Red, far-red response & chlorophyll synthesis. Plant Physiol. 1961 Nov;36(6):733–735. doi: 10.1104/pp.36.6.733. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. WOLFF J. B., PRICE L. Terminal steps of chlorophyll A biosynthesis in higher plants. Arch Biochem Biophys. 1957 Dec;72(2):293–301. doi: 10.1016/0003-9861(57)90205-9. [DOI] [PubMed] [Google Scholar]
  15. Withrow R. B., Klein W. H., Price L., Elstad V. Influence of Visible and Near Infrared Radiant Energy on Organ Development and Pigment Synthesis in Bean and Corn. Plant Physiol. 1953 Jan;28(1):1–14. doi: 10.1104/pp.28.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES