Abstract
Two enzyme-linked immunosorbent assays for antigen quantitation, direct competition and antibody inhibition, were used to measure rabbit immunoglobulin G in polystyrene microtiter plates and were compared for sensitivity and reproducibility. Both procedures repetitively detected this antigen in the 1- to 100-ng/ml range. Both procedures were predictably reproducible, with direct competition procedures having steeper slopes in ranges tested. Antibody inhibition does not require conjugated antigen and can detect sample antigens if available stock antigens can be passively bound to a solid-phase polystyrene plate.
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Selected References
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- Avrameas S., Ternynck T. The cross-linking of proteins with glutaraldehyde and its use for the preparation of immunoadsorbents. Immunochemistry. 1969 Jan;6(1):53–66. doi: 10.1016/0019-2791(69)90178-5. [DOI] [PubMed] [Google Scholar]
- Catt K., Tregear G. W. Solid-phase radioimmunoassay in antibody-coated tubes. Science. 1967 Dec 22;158(3808):1570–1572. doi: 10.1126/science.158.3808.1570. [DOI] [PubMed] [Google Scholar]
- Engvall E., Jonsson K., Perlmann P. Enzyme-linked immunosorbent assay. II. Quantitative assay of protein antigen, immunoglobulin G, by means of enzyme-labelled antigen and antibody-coated tubes. Biochim Biophys Acta. 1971 Dec 28;251(3):427–434. doi: 10.1016/0005-2795(71)90132-2. [DOI] [PubMed] [Google Scholar]
- Hammarström S., Engvall E., Johansson B. G., Svensson S., Sundblad G., Goldstein I. J. Nature of the tumor-associated determinant(s) of carcinoembryonic antigen. Proc Natl Acad Sci U S A. 1975 Apr;72(4):1528–1532. doi: 10.1073/pnas.72.4.1528. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Miedema K., Boelhouwer J., Otten J. W. Determinations of proteins and hormones in serum by an immunoassay using antigen-enzyme conjugates. Clin Chim Acta. 1972 Aug;40(1):187–192. doi: 10.1016/0009-8981(72)90269-0. [DOI] [PubMed] [Google Scholar]
- Stiffler-Rosenberg G., Fey H. Simple assay for staphylococcal enterotoxins A, B, and C: modification of enzyme-linked immunosorbent assay. J Clin Microbiol. 1978 Nov;8(5):473–479. doi: 10.1128/jcm.8.5.473-479.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wolters G., Kuijpers L., Kacaki J., Schuurs A. Solid-phase enzyme-immunoassay for detection of hepatitis B surface antigen. J Clin Pathol. 1976 Oct;29(10):873–879. doi: 10.1136/jcp.29.10.873. [DOI] [PMC free article] [PubMed] [Google Scholar]