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. 1980 Feb;27(2):643–649. doi: 10.1128/iai.27.2.643-649.1980

Effects of Activated Macrophages on Nocardia asteroides

Gregory A Filice 1,2, Blaine L Beaman 2, Jack S Remington 1,2
PMCID: PMC550813  PMID: 6991421

Abstract

The mechanism(s) of host resistance against Nocardia asteroides has not been well defined. Since disease due to N. asteroides frequently occurs in patients with impaired cell-mediated immunity, we studied the interaction of N. asteroides with activated and control mouse peritoneal macrophages. Activated macrophages were from mice infected with Toxoplasma gondii or injected with Corynebacterium parvum. N. asteroides in the early stationary phase (>99% in the coccobacillary form) was used for challenge of macrophage monolayers. Growth of two strains of N. asteroides was markedly inhibited in activated macrophages, whereas N. asteroides grew well in control macrophages. Quantitation of macrophage-associated N. asteroides indicated that activated macrophages killed 40 to 50% of N. asteroides within 6 h (P < 0.002). In control macrophage preparations, it appeared as if Nocardia filaments extended from within macrophages to the outside, and many of these filaments appeared to have extended to and then grown through neighboring macrophages. In activated macrophage preparations, Nocardia remained in the coccobacillary form in most macrophages. Control macrophage monolayers were almost completely overgrown with and destroyed by Nocardia 20 h after challenge, whereas activated macrophage monolayers remained intact. Nocardia that grew in control macrophages were not acid-alcohol fast or only weakly so, whereas the few Nocardia that grew in activated macrophages were strongly acid-alcohol fast. Our results indicate that activated macrophages may be important in host defense against N. asteroides.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson S. E., Jr, Remington J. S. Effect of normal and activated human macrophages on Toxoplasma gondii. J Exp Med. 1974 May 1;139(5):1154–1174. doi: 10.1084/jem.139.5.1154. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Babior B. M. Oxygen-dependent microbial killing by phagocytes (second of two parts). N Engl J Med. 1978 Mar 30;298(13):721–725. doi: 10.1056/NEJM197803302981305. [DOI] [PubMed] [Google Scholar]
  3. Beaman B. L., Burnside J., Edwards B., Causey W. Nocardial infections in the United States, 1972-1974. J Infect Dis. 1976 Sep;134(3):286–289. doi: 10.1093/infdis/134.3.286. [DOI] [PubMed] [Google Scholar]
  4. Beaman B. L., Gershwin M. E., Maslan S. Infectious agents in immunodeficient murine models: pathogenicity of Nocardia asteroides in congenitally athymic (nude) and hereditarily asplenic (Dh/+) mice. Infect Immun. 1978 May;20(2):381–387. doi: 10.1128/iai.20.2.381-387.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Beaman B. L. In vitro response of rabbit alveolar macrophages to infection with Nocardia asteroides. Infect Immun. 1977 Mar;15(3):925–937. doi: 10.1128/iai.15.3.925-937.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Beaman B. L. Interaction of Nocardia asteroides at different phases of growth with in vitro-maintained macrophages obtained from the lungs of normal and immunized rabbits. Infect Immun. 1979 Oct;26(1):355–361. doi: 10.1128/iai.26.1.355-361.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Beaman B. L., Maslan S. Effect of cyclophosphamide on experimental Nocardia asteroides infection in mice. Infect Immun. 1977 Jun;16(3):995–1004. doi: 10.1128/iai.16.3.995-1004.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Beaman B. L., Maslan S. Virulence of Nocardia asteroides during its growth cycle. Infect Immun. 1978 Apr;20(1):290–295. doi: 10.1128/iai.20.1.290-295.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Beaman B. L., Smathers M. Interaction of Nocardia asteroides with cultured rabbit alveolar macrophages. Infect Immun. 1976 Apr;13(4):1126–1131. doi: 10.1128/iai.13.4.1126-1131.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Bourgeois L., Beaman B. L. Probable L-forms of Nocardia asteroides induced in cultured mouse peritoneal macrophages. Infect Immun. 1974 Mar;9(3):576–590. doi: 10.1128/iai.9.3.576-590.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Folb P. I., Timme A., Horowitz A. Nocardia infections in congenitally athymic (nude) mice and in other inbred mouse strains. Infect Immun. 1977 Nov;18(2):459–466. doi: 10.1128/iai.18.2.459-466.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. GORRILL R. H., HEPTINSTALL R. H. The animal pathogenicity of Nocardia sebivorans nov. spec. J Pathol Bacteriol. 1954 Oct;68(2):387–393. doi: 10.1002/path.1700680211. [DOI] [PubMed] [Google Scholar]
  13. Hibbs J. B., Jr, Lambert L. H., Jr, Remington J. S. Resistance to murine tumors conferred by chronic infection with intracellular protozoa, Toxoplasma gondii and Besnoitia jellisoni. J Infect Dis. 1971 Dec;124(6):587–592. doi: 10.1093/infdis/124.6.587. [DOI] [PubMed] [Google Scholar]
  14. Hoidal J. R., Fox R. B., Repine J. E. Defective oxidative metabolic responses in vitro of alveolar macrophages in chronic granulomatous disease. Am Rev Respir Dis. 1979 Sep;120(3):613–618. doi: 10.1164/arrd.1979.120.3.613. [DOI] [PubMed] [Google Scholar]
  15. Idriss Z. H., Cunningham R. J., Wilfert C. M. Nocardiosis in children: report of three cases and review of the literature. Pediatrics. 1975 Apr;55(4):479–484. [PubMed] [Google Scholar]
  16. Krahenbuhl J. L., Lambert L. H., Jr, Remington J. S. The effects of activated macrophages on tumor target cells: escape from cytostasis. Cell Immunol. 1976 Aug;25(2):279–293. doi: 10.1016/0008-8749(76)90118-0. [DOI] [PubMed] [Google Scholar]
  17. Krick J. A., Remington J. S. Resistance to infection with Nocardia asteroides. J Infect Dis. 1975 Jun;131(6):665–672. doi: 10.1093/infdis/131.6.665. [DOI] [PubMed] [Google Scholar]
  18. Krick J. A., Stinson E. B., Remington J. S. Nocardia infection in heart transplant patients. Ann Intern Med. 1975 Jan;82(1):18–26. doi: 10.7326/0003-4819-82-1-18. [DOI] [PubMed] [Google Scholar]
  19. MACKANESS G. B. Cellular resistance to infection. J Exp Med. 1962 Sep 1;116:381–406. doi: 10.1084/jem.116.3.381. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. McLeod R., Remington J. S. Influence of infection with Toxoplasma on macrophage function, and role of macrophages in resistance to Toxoplasma. Am J Trop Med Hyg. 1977 Nov;26(6 Pt 2):170–186. doi: 10.4269/ajtmh.1977.26.170. [DOI] [PubMed] [Google Scholar]
  21. McLeod R., Remington J. S. Studies on the specificity of killing of intracellular pathogens by macrophages. Cell Immunol. 1977 Nov;34(1):156–174. doi: 10.1016/0008-8749(77)90238-6. [DOI] [PubMed] [Google Scholar]
  22. Palmer D. L., Harvey R. L., Wheeler J. K. Diagnostic and therapeutic considerations in Nocardia asteroides infection. Medicine (Baltimore) 1974 Sep;53(5):391–401. doi: 10.1097/00005792-197409000-00005. [DOI] [PubMed] [Google Scholar]
  23. Splino M., Merka V., Kyntera F. Phagozytose und intrazelluläre Proliferation von Nocardia asteroides (Stamm Weipheld) in Zellstrukturen in vitro. 1. Alveolare Makrophagen von Meerschweinchen. Zentralbl Bakteriol Orig A. 1975 Jul;232(2-3):334–340. [PubMed] [Google Scholar]
  24. Splino M., Merka V., Kyntera F. Phagozytose und intrazelluläre Proliferation von Nocardia asteroides (Stamm Weipheld) in Zellstrukturen in vitro. 2. Peritoneale Makrophagen von Meerschweinchen. Zentralbl Bakteriol Orig A. 1976 Aug;235(4):512–520. [PubMed] [Google Scholar]
  25. Sundararaj T., Agarwal S. C. Cell-mediated immunity in experimental Nocardia asteroides infection. Infect Immun. 1977 Feb;15(2):370–375. doi: 10.1128/iai.15.2.370-375.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Sundararaj T., Agarwal S. C. Cell-mediated immunity to Nocardia asteroides induced by its ribonucleic acid protein fraction. Infect Immun. 1977 Oct;18(1):253–256. doi: 10.1128/iai.18.1.253-256.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Sundararaj T., Agarwal S. C. Relationship of macrophages to cell-mediated immunity in experimental Nocardia asteroides infection. Infect Immun. 1978 Jun;20(3):685–691. doi: 10.1128/iai.20.3.685-691.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Wing E. J., Remington J. S. Studies on the regulation of lymphocyte reactivity by normal and activated macrophages. Cell Immunol. 1977 Apr;30(1):108–121. doi: 10.1016/0008-8749(77)90052-1. [DOI] [PubMed] [Google Scholar]
  29. Young L. S., Armstrong D., Blevins A., Lieberman P. Nocardia asteroides infection complicating neoplastic disease. Am J Med. 1971 Mar;50(3):356–367. doi: 10.1016/0002-9343(71)90224-5. [DOI] [PubMed] [Google Scholar]

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