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. 1980 Mar;27(3):938–946. doi: 10.1128/iai.27.3.938-946.1980

Hemagglutination properties and adherence ability of Aeromonas hydrophila.

H M Atkinson, T J Trust
PMCID: PMC550865  PMID: 6103874

Abstract

Human diarrheal isolates and enterotoxigenic strains of Aeromonas hydrophila are strong hemagglutinators with human blood cells. Sugar inhibition studies and yeast coagglutination tests with 11 selected strains revealed six different hemagglutination mechanisms for this species. These were characterized by inhibition by L-fucose, inhibition by D-galactose, inhibition by D-mannose, and two distinguishable mechanisms which were inhibited by either L-fucose or D-mannose, one being pilus mediated. Inhibition of hemagglutination by another strain required a combination of D-galactose and D-mannose. The hemagglutinating strains also attached well to human blood cells and buccal epithelial cells, with as many as 55% of the cells of a culture attaching successfully. In some cases the attachment to buccal epithelial cells appeared to involve mechanisms different from those used for hemagglutination.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Annapurna E., Sanyal S. C. Enterotoxicity of Aeromonas hydrophila. J Med Microbiol. 1977 Aug;10(3):317–323. doi: 10.1099/00222615-10-3-317. [DOI] [PubMed] [Google Scholar]
  2. Cumberbatch N., Gurwith M. J., Langston C., Sack R. B., Brunton J. L. Cytotoxic enterotoxin produced by Aeromonas hydrophila: relationship of toxigenic isolates to diarrheal disease. Infect Immun. 1979 Mar;23(3):829–837. doi: 10.1128/iai.23.3.829-837.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. DUGUID J. P. Fimbriae and adhesive properties in Klebsiella strains. J Gen Microbiol. 1959 Aug;21:271–286. doi: 10.1099/00221287-21-1-271. [DOI] [PubMed] [Google Scholar]
  4. Davis W. A., 2nd, Kane J. G., Garagusi V. F. Human aeromonas infections: a review of the literature and a case report of endocarditis. Medicine (Baltimore) 1978 May;57(3):267–277. [PubMed] [Google Scholar]
  5. Duguid J. P., Anderson E. S., Campbell I. Fimbriae and adhesive properties in Salmonellae. J Pathol Bacteriol. 1966 Jul;92(1):107–138. doi: 10.1002/path.1700920113. [DOI] [PubMed] [Google Scholar]
  6. Eshdat Y., Ofek I., Yashouv-Gan Y., Sharon N., Mirelman D. Isolation of a mannose-specific lectin from Escherichia coli and its role in the adherence of the bacteria to epithelial cells. Biochem Biophys Res Commun. 1978 Dec 29;85(4):1551–1559. doi: 10.1016/0006-291x(78)91179-8. [DOI] [PubMed] [Google Scholar]
  7. Freter R., Jones G. W. Adhesive properties of Vibrio cholerae: nature of the interaction with intact mucosal surfaces. Infect Immun. 1976 Jul;14(1):246–256. doi: 10.1128/iai.14.1.246-256.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Fulghum D. D., Linton W. R., Taplin D. Fatal Aeromonas hydrophila infection of the skin. South Med J. 1978 Jun;71(6):739–741. doi: 10.1097/00007611-197806000-00037. [DOI] [PubMed] [Google Scholar]
  9. Gilboa-Garber Nechama. Inhibition of broad spectrum hemagglutinin from Pseudomonas aeruginosa by D-galactose and its derivatives. FEBS Lett. 1972 Feb 1;20(2):242–244. doi: 10.1016/0014-5793(72)80805-6. [DOI] [PubMed] [Google Scholar]
  10. Gorbach S. L., Banwell J. G., Jacobs B., Chatterjee B. D., Mitra R., Brigham K. L., Neogy K. N. Intestinal microflora in Asiatic cholera. II. The small bowel. J Infect Dis. 1970 Jan;121(1):38–45. doi: 10.1093/infdis/121.1.38. [DOI] [PubMed] [Google Scholar]
  11. Hohmann A., Wilson M. R. Adherence of enteropathogenic Escherichia coli to intestinal epithelium in vivo. Infect Immun. 1975 Oct;12(4):866–880. doi: 10.1128/iai.12.4.866-880.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Jones G. W., Freter R. Adhesive properties of Vibrio cholerae: nature of the interaction with isolated rabbit brush border membranes and human erythrocytes. Infect Immun. 1976 Jul;14(1):240–245. doi: 10.1128/iai.14.1.240-245.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Ketover B. P., Young L. S., Armstrong D. Septicemia due to Aeromonas hydrophila: clinical and immunologic aspects. J Infect Dis. 1973 Mar;127(3):284–290. doi: 10.1093/infdis/127.3.284. [DOI] [PubMed] [Google Scholar]
  14. Lambden P. R., Heckels J. E., James L. T., Watt P. J. Variations in surface protein composition associated with virulence properties in opacity types of Neisseria gonorrhoeae. J Gen Microbiol. 1979 Oct;114(2):305–312. doi: 10.1099/00221287-114-2-305. [DOI] [PubMed] [Google Scholar]
  15. Ljungh A., Popoff M., Wadstrom T. Aeromonas hydrophila in acute diarrheal disease: detection of enterotoxin and biotyping of strains. J Clin Microbiol. 1977 Aug;6(2):96–100. doi: 10.1128/jcm.6.2.96-100.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. MacInnes J. I., Trust T. J., Crosa J. H. Deoxyribonucleic acid relationships among members of the genus Aeromonas. Can J Microbiol. 1979 May;25(5):579–586. doi: 10.1139/m79-083. [DOI] [PubMed] [Google Scholar]
  17. Moon H. W., Isaacson R. E., Pohlenz J. Mechanisms of association of enteropathogenic Escherichia coli with intestinal epithelium. Am J Clin Nutr. 1979 Jan;32(1):119–127. doi: 10.1093/ajcn/32.1.119. [DOI] [PubMed] [Google Scholar]
  18. Nakajima T., Ballou C. E. Characterization of the carbohydrate fragments obtained from Saccharomyces cerevisiae mannan by alkaline degradation. J Biol Chem. 1974 Dec 10;249(23):7679–7684. [PubMed] [Google Scholar]
  19. Old D. C. Inhibition of the interaction between fimbrial haemagglutinins and erythrocytes by D-mannose and other carbohydrates. J Gen Microbiol. 1972 Jun;71(1):149–157. doi: 10.1099/00221287-71-1-149. [DOI] [PubMed] [Google Scholar]
  20. Trust T. J., Chipman D. C. Clinical involvement of Aeromonas hydrophila. Can Med Assoc J. 1979 Apr 21;120(8):942–946. [PMC free article] [PubMed] [Google Scholar]
  21. Tweedy J. M., Park R. W., Hodgkiss W. Evidence for the presence of fimbriae (pili) on vibrio species. J Gen Microbiol. 1968 Apr;51(2):235–244. doi: 10.1099/00221287-51-2-235. [DOI] [PubMed] [Google Scholar]
  22. Von Graevenitz A., Mensch A. H. The genus aeromonas in human bacteriology report of 30 cases and review of the literature. N Engl J Med. 1968 Feb 1;278(5):245–249. doi: 10.1056/NEJM196802012780504. [DOI] [PubMed] [Google Scholar]

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