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. 1980 May;28(2):411–416. doi: 10.1128/iai.28.2.411-416.1980

Demonstration of cell envelope-bound exotoxin A in Pseudomonas aeruginosa.

P B Fernandes, K R Cundy
PMCID: PMC550950  PMID: 6249740

Abstract

The quantity of membrane-bound and extracellular exotoxin A in four strains of Pseudomonas aeruginosa was investigated. In strain PA-103, which is the prototype strain used for toxin production, all of the toxin was released into the growth medium and little toxin remained with the cell envelope. In a virulent strain from a clinical source, strain 119, exotoxin A was found in equal amounts in the growth medium and in the cell envelopes. An avirulent mutant of this strain, strain 119 (AP-), which was resistant to 800 microgram of polymyxin B per ml, had all the exotoxin A in a membrane-bound state and was unable to release exotoxin A into the growth medium. Thus, exotoxin A can be found in the membrane-bound form, in the extracellular form, or in both. The quantities of membrane-bound toxin and extracellular toxin vary with the strain P. aeruginosa. The polymyxin B-resistant mutant which is blocked in toxin release will be useful in studies of exotoxin A secretion.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brown M. R., Watkins W. M. Low magnesium and phospholipid content of cell wals of Pseudomonas aeruginosa resistant to polymyxin. Nature. 1970 Sep 26;227(5265):1360–1361. doi: 10.1038/2271360a0. [DOI] [PubMed] [Google Scholar]
  2. Chung D. W., Collier R. J. Enzymatically active peptide from the adenosine diphosphate-ribosylating toxin of Pseudomonas aeruginosa. Infect Immun. 1977 Jun;16(3):832–841. doi: 10.1128/iai.16.3.832-841.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Dorrer E., Teuber M. Induction of polymyxin resistance in Pseudomonas fluorescens by phosphate limitation. Arch Microbiol. 1977 Jul 26;114(1):87–89. doi: 10.1007/BF00429636. [DOI] [PubMed] [Google Scholar]
  4. Fernandes P. B., Bayer M. E. Membrane-bound enterotoxin of Vibrio cholerae. J Gen Microbiol. 1977 Dec;103(2):381–387. doi: 10.1099/00221287-103-2-381. [DOI] [PubMed] [Google Scholar]
  5. Fernandes P. B., Welsh K. M., Bayer M. E. Characterization of membrane-bound nicotinamide adenine dinucleotide glycohydrolase of Vibrio cholerae. J Biol Chem. 1979 Sep 25;254(18):9254–9261. [PubMed] [Google Scholar]
  6. Gilleland H. E., Jr, Lyle R. D. Chemical alterations in cell envelopes of polymyxin-resistant Pseudomonas aeruginosa isolates. J Bacteriol. 1979 Jun;138(3):839–845. doi: 10.1128/jb.138.3.839-845.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Iglewski B. H., Kabat D. NAD-dependent inhibition of protein synthesis by Pseudomonas aeruginosa toxin,. Proc Natl Acad Sci U S A. 1975 Jun;72(6):2284–2288. doi: 10.1073/pnas.72.6.2284. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Iglewski B. H., Liu P. V., Kabat D. Mechanism of action of Pseudomonas aeruginosa exotoxin Aiadenosine diphosphate-ribosylation of mammalian elongation factor 2 in vitro and in vivo. Infect Immun. 1977 Jan;15(1):138–144. doi: 10.1128/iai.15.1.138-144.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Iglewski B. H., Sadoff J., Bjorn M. J., Maxwell E. S. Pseudomonas aeruginosa exoenzyme S: an adenosine diphosphate ribosyltransferase distinct from toxin A. Proc Natl Acad Sci U S A. 1978 Jul;75(7):3211–3215. doi: 10.1073/pnas.75.7.3211. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  11. Liu P. V. Exotoxins of Pseudomonas aeruginosa. I. Factors that influence the production of exotoxin A. J Infect Dis. 1973 Oct;128(4):506–513. doi: 10.1093/infdis/128.4.506. [DOI] [PubMed] [Google Scholar]
  12. Liu P. V., Yoshii S., Hsieh H. Exotoxins of Pseudomonas aeruginosa. II. Concentration, purification, and characterization of exotoxin A. J Infect Dis. 1973 Oct;128(4):514–519. doi: 10.1093/infdis/128.4.514. [DOI] [PubMed] [Google Scholar]
  13. MORIHARA K. PRODUCTION OF ELASTASE AND PROTEINASE BY PSEUDOMONAS AERUGINOSA. J Bacteriol. 1964 Sep;88:745–757. doi: 10.1128/jb.88.3.745-757.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Morihara K., Tsuzuki H. Substrate specificity of elastolytic and nonelastolytic proteinases from Pseudomonas aeruginosa. Arch Biochem Biophys. 1966 Apr;114(1):158–165. doi: 10.1016/0003-9861(66)90317-1. [DOI] [PubMed] [Google Scholar]
  15. Moss J., Manganiello V. C., Vaughan M. Hydrolysis of nicotinamide adenine dinucleotide by choleragen and its A protomer: possible role in the activation of adenylate cyclase. Proc Natl Acad Sci U S A. 1976 Dec;73(12):4424–4427. doi: 10.1073/pnas.73.12.4424. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Pavlovskis O. R., Wretlind B. Assessment of protease (elastase) as a Pseudomonas aeruginosa virulence factor in experimental mouse burn infection. Infect Immun. 1979 Apr;24(1):181–187. doi: 10.1128/iai.24.1.181-187.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Vasil M. L., Kabat D., Iglewski B. H. Structure-activity relationships of an exotoxin of Pseudomonas aeruginosa. Infect Immun. 1977 Apr;16(1):353–361. doi: 10.1128/iai.16.1.353-361.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]

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