Abstract
A significant proportion (20 to 40%) of highly purified 22-nm hepatitis B surface antigen (HBsAg) particles contain human serum albumin (HSA) as demonstrated by specific precipitation of radioiodinated particles by anti-HSA. Preparations of the isolated major HBsAg polypeptides (P-1, P-2, and P-6) were iodinated and analyzed by radiommunoprecipitation for reactivity with rabbit antisera to human plasma proteins. Only the P-6 fraction (molecular weight, 68,000) was precipitated and only by anti-HSA; specific precipitation was observed with guinea pig antisera to P-6 and native HBsAg and goat or rabbit antisera to HSA. Coprecipitation of P-6 with antiserum to HBsAg and with anti-HSA, compared to precipitation with each antiserum alone, indicated that the HBsAg and HSA determinants were on separate molecules. The P-6 polypeptide may represent a precursor protein of the hepatitis B virion.
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- Burrell C. J. Host components in hepatitis B antigen. J Gen Virol. 1975 May;27(2):117–126. doi: 10.1099/0022-1317-27-2-117. [DOI] [PubMed] [Google Scholar]
- Burrell C. J., Mackay P., Greenaway P. J., Hofschneider P. H., Murray K. Expression in Escherichia coli of hepatitis B virus DNA sequences cloned in plasmid pBR322. Nature. 1979 May 3;279(5708):43–47. doi: 10.1038/279043a0. [DOI] [PubMed] [Google Scholar]
- Charnay P., Pourcel C., Louise A., Fritsch A., Tiollais P. Cloning in Escherichia coli and physical structure of hepatitis B virion DNA. Proc Natl Acad Sci U S A. 1979 May;76(5):2222–2226. doi: 10.1073/pnas.76.5.2222. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gerin J. L., Faust R. M., Holland P. V. Biophysical characterization of the adr subtype of hepatitis B antigen and preparation of anti-r sera in rabbits. J Immunol. 1975 Jul;115(1):100–105. [PubMed] [Google Scholar]
- Gerin J. L., Holland P. V., Purcell R. H. Australia antigen: large-scale purification from human serum and biochemical studies of its proteins. J Virol. 1971 May;7(5):569–576. doi: 10.1128/jvi.7.5.569-576.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hollinger F. B., Dressman G. R. Hepatitis B virus antigens and albumin receptors. Gastroenterology. 1979 Mar;76(3):641–643. [PubMed] [Google Scholar]
- Imai M., Yanase Y., Nojiri T., Miyakawa Y., Mayumi M. A receptor for polymerized human and chimpanzee albumins on hepatitis B virus particles co-occurring with HBeAg. Gastroenterology. 1979 Feb;76(2):242–247. [PubMed] [Google Scholar]
- Metzger H., Shapiro M. B., Mosimann J. E., Vinton J. E. Assessment of compositional relatedness between proteins. Nature. 1968 Sep 14;219(5159):1166–1168. doi: 10.1038/2191166a0. [DOI] [PubMed] [Google Scholar]
- Neurath A. R., Prince A. M., Lippin A. Hepatitis B antigen: antigenic sites related to human serum proteins revealed by affinity chromatography. Proc Natl Acad Sci U S A. 1974 Jul;71(7):2663–2667. doi: 10.1073/pnas.71.7.2663. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Neurath A. R., Strick N. Radioimmunoassay for albumin-binding sites associated with HBsAg: correlation of results with the presence of e-antigen in serum. Intervirology. 1979;11(2):128–132. doi: 10.1159/000149024. [DOI] [PubMed] [Google Scholar]
- Peterson D. L., Roberts I. M., Vyas G. N. Partial amino acid sequence of two major component polypeptides of hepatitis B surface antigen. Proc Natl Acad Sci U S A. 1977 Apr;74(4):1530–1534. doi: 10.1073/pnas.74.4.1530. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schuurs A. H., Wolters G. Hepatitis B surface antigen and human serum proteins. Am J Med Sci. 1975 Jul-Aug;270(1):173–177. doi: 10.1097/00000441-197507000-00024. [DOI] [PubMed] [Google Scholar]
- Shih J. W., Gerin J. L. Immunochemistry of hepatitis B surface antigen (HBsAg): preparation and characterization of antibodies to the constituent polypeptides. J Immunol. 1975 Sep;115(3):634–639. [PubMed] [Google Scholar]
- Shih J. W., Gerin J. L. Proteins of hepatitis B surface antigen. J Virol. 1977 Jan;21(1):347–357. doi: 10.1128/jvi.21.1.347-357.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Shih J. W., Gerin J. L. Proteins of hepatitis B surface antigen: amino acid compositions of the major polypeptides. J Virol. 1977 Mar;21(3):1219–1222. doi: 10.1128/jvi.21.3.1219-1222.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Shih J. W., Tan P. L., Gerin J. L. Antigenicity of the major polypeptides of hepatitis B surface antigen (HBsAg). J Immunol. 1978 Feb;120(2):520–525. [PubMed] [Google Scholar]
- Sninsky J. J., Siddiqui A., Robinson W. S., Cohen S. N. Cloning and endonuclease mapping of the hepatitis B viral genome. Nature. 1979 May 24;279(5711):346–348. doi: 10.1038/279346a0. [DOI] [PubMed] [Google Scholar]