Abstract
The rfb locus, determining biosynthesis of O8-specific lipopolysaccharide side chains, was transferred to a rough mutant of Escherichia coli; recombinants producing a complete lipopolysaccharide were more resistant to the complement-mediated bactericidal action of human serum than the rough recipient. Inheritance of the his-linked genes for K27 antigen production did not alter the response to serum. The serum resistance of strains carrying O8 side chains, but not of strains with incomplete lipopolysaccharides, was further increased by inheritance of plasmids R1 and NR1.20
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- Binns M. M., Davies D. L., Hardy K. G. Cloned fragments of the plasmid ColV,I-K94 specifying virulence and serum resistance. Nature. 1979 Jun 28;279(5716):778–781. doi: 10.1038/279778a0. [DOI] [PubMed] [Google Scholar]
- Feingold D. S. The serum bactericidal reaction. IV. Phenotypic conversion of Escherichia coli from serum-resistance to serum-sensitivity by diphenylamine. J Infect Dis. 1969 Oct;120(4):437–444. doi: 10.1093/infdis/120.4.437. [DOI] [PubMed] [Google Scholar]
- Fietta A., Romero E., Siccardi A. G. Effect of some R factors on the sensitivity of rough Enterobacteriaceae to human serum. Infect Immun. 1977 Nov;18(2):278–282. doi: 10.1128/iai.18.2.278-282.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Glynn A. A., Howard C. J. The sensitivity to complement of strains of Escherichia coli related to their K antigens. Immunology. 1970 Mar;18(3):331–346. [PMC free article] [PubMed] [Google Scholar]
- Inoue K., Yonemasu K., Takamizawa A., Amano T. [Studies on the immune bacteriolysis. XIV. Requirement of all nine components of complement for immune bacteriolysis]. Biken J. 1968 Sep;11(3):203–206. [PubMed] [Google Scholar]
- Olson A. C., Schmidt G., Jann K. Biochemistry of the K antigens of Escherichia coli. Formation of the nucleoside diphosphate sugar precursors of the K27 antigen of E. coli 08:K27(A):H-. Eur J Biochem. 1969 Dec;11(2):376–385. doi: 10.1111/j.1432-1033.1969.tb00783.x. [DOI] [PubMed] [Google Scholar]
- Reske K., Jann K. The O8 antigen of Escherichia coli. Structure of the polysaccharide chain. Eur J Biochem. 1972 Dec 4;31(2):320–328. doi: 10.1111/j.1432-1033.1972.tb02536.x. [DOI] [PubMed] [Google Scholar]
- Reynard A. M., Beck M. E. Plasmid-mediated resistance to the bactericidal effects of normal rabbit serum. Infect Immun. 1976 Sep;14(3):848–850. doi: 10.1128/iai.14.3.848-850.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schmidt G., Fromme I., Mayer H. Immunochemical studies on core lipopolysaccharides of Enterobacteriaceae of different genera. Eur J Biochem. 1970 Jun;14(2):357–366. doi: 10.1111/j.1432-1033.1970.tb00297.x. [DOI] [PubMed] [Google Scholar]
- Schmidt G., Jann B., Jann K. Immunochemistry of R lipopolysaccharides of Escherichia coli. Different core regions in the lipopolysaccharides of O group 8. Eur J Biochem. 1969 Oct;10(3):501–510. doi: 10.1111/j.1432-1033.1969.tb00717.x. [DOI] [PubMed] [Google Scholar]
- Schmidt G., Jann B., Jann K., Orskov I., Orskov F. Genetic determinants of the synthesis of the polysaccharide capsular antigen K27(A) of Escherichia coli. J Gen Microbiol. 1977 Jun;100(2):355–361. doi: 10.1099/00221287-100-2-355. [DOI] [PubMed] [Google Scholar]
- Schmidt G. Konjugation bei E. coli O8:K27(A): H minus. Zentralbl Bakteriol Orig. 1969;211(3):335–344. [PubMed] [Google Scholar]
- Taylor P. W. Genetical studies of serum resistance in Escherichia coli. J Gen Microbiol. 1975 Jul;89(1):57–66. doi: 10.1099/00221287-89-1-57. [DOI] [PubMed] [Google Scholar]
- Taylor P. W., Hughes C. Plasmid carriage and the serum sensitivity of enterobacteria. Infect Immun. 1978 Oct;22(1):10–17. doi: 10.1128/iai.22.1.10-17.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Taylor P. W., Parton R. A protein factor associated with serum resistance in Escherichia coli. J Med Microbiol. 1977 May;10(2):225–232. doi: 10.1099/00222615-10-2-225. [DOI] [PubMed] [Google Scholar]