Skip to main content
PMC home page
Infection and Immunity logoLink to Infection and Immunity
. 1980 Aug;29(2):335–341. doi: 10.1128/iai.29.2.335-341.1980

Influence of complement on the chemiluminescent response of human leukocytes to immune complex.

S Löfgren, A Tärnvik, J Carlsson
PMCID: PMC551122  PMID: 7011971

Abstract

Vaccine bacteria of Francisella tularensis were mixed with human serum containing specific antibodies against the same organism, and the mixture was incubated at 37 degrees C for 30 min. The mixture induced a two-peak chemiluminescent response in polymorphonuclear leukocytes. The initial peak was induced by a soluble agent, formed during incubation. The formation of this agent involved the activation of complement component C5. The second peak of the chemiluminescent response was induced by the opsonized bacteria; this peak was augmented by complement component C3, whereas C5 had no influence.

Full text

PDF
339

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Allen R. C., Stjernholm R. L., Steele R. H. Evidence for the generation of an electronic excitation state(s) in human polymorphonuclear leukocytes and its participation in bactericidal activity. Biochem Biophys Res Commun. 1972 May 26;47(4):679–684. doi: 10.1016/0006-291x(72)90545-1. [DOI] [PubMed] [Google Scholar]
  2. Allred C. D., Hill H. R. Effect of chemoattractants on chemiluminescence. Infect Immun. 1978 Mar;19(3):833–838. doi: 10.1128/iai.19.3.833-838.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Allred C. D., Shigeoka A. O., Hill H. R. Evaluation of group B streptococcal opsonins by radiolabeled bacterial uptake. J Immunol Methods. 1979;26(4):355–363. doi: 10.1016/0022-1759(79)90181-9. [DOI] [PubMed] [Google Scholar]
  4. Andersen B. R., Brendzel A. M., Lint T. F. Chemiluminescence spectra of human myeloperoxidase and polymorphonuclear leukocytes. Infect Immun. 1977 Jul;17(1):62–66. doi: 10.1128/iai.17.1.62-66.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Andersen B. R., Brendzel A. M. Use of a unique chemiluminescence spectrometer in a study of factors influencing granulocyte light emission. J Immunol Methods. 1978;19(2-3):279–287. doi: 10.1016/0022-1759(78)90187-4. [DOI] [PubMed] [Google Scholar]
  6. Cheson B. D., Christensen R. L., Sperling R., Kohler B. E., Babior B. M. The origin of the chemiluminescence of phagocytosing granulocytes. J Clin Invest. 1976 Oct;58(4):789–796. doi: 10.1172/JCI108530. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. DIOGUARDI N., AGOSTONI A., FIORELLI G., LOMANTO B. Characterization of lactic dehydrogenase of normal human granulocytes. J Lab Clin Med. 1963 May;61:713–723. [PubMed] [Google Scholar]
  8. EIGELSBACH H. T., DOWNS C. M. Prophylactic effectiveness of live and killed tularemia vaccines. I. Production of vaccine and evaluation in the white mouse and guinea pig. J Immunol. 1961 Oct;87:415–425. [PubMed] [Google Scholar]
  9. Goldstein I. M., Roos D., Kaplan H. B., Weissmann G. Complement and immunoglobulins stimulate superoxide production by human leukocytes independently of phagocytosis. J Clin Invest. 1975 Nov;56(5):1155–1163. doi: 10.1172/JCI108191. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Grebner J. V., Mills E. L., Gray G. H., Quie P. G. Comparison of phagocytic and chemiluminescence response of human polymorphonuclear neutrophils. J Lab Clin Med. 1977 Jan;89(1):153–159. [PubMed] [Google Scholar]
  11. Harvath L., Amirault H. J., Andersen B. R. Chemiluminescence of human and canine polymorphonuclear leukocytes in the absence of phagocytosis. J Clin Invest. 1978 May;61(5):1145–1154. doi: 10.1172/JCI109029. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Harvath L., Andersen B. R. Defective initiation of oxidative metabolism in polymorphonuclear leukocytes. N Engl J Med. 1979 May 17;300(20):1130–1135. doi: 10.1056/NEJM197905173002003. [DOI] [PubMed] [Google Scholar]
  13. Hatch G. E., Gardner D. E., Menzel D. B. Chemiluminescence of phagocytic cells caused by N-formylmethionyl peptides. J Exp Med. 1978 Jan 1;147(1):182–195. doi: 10.1084/jem.147.1.182. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Hemming V. G., Hall R. T., Rhodes P. G., Shigeoka A. O., Hill H. R. Assessment of group B streptococcal opsonins in human and rabbit serum by neutrophil chemiluminescence. J Clin Invest. 1976 Dec;58(6):1379–1387. doi: 10.1172/JCI108593. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Hill H. R., Hogan N. A., Bale J. F., Hemming V. G. Evaluation of nonspecific (alternative pathway) opsonic activity by neutrophil chemiluminescence. Int Arch Allergy Appl Immunol. 1977;53(6):490–497. doi: 10.1159/000231790. [DOI] [PubMed] [Google Scholar]
  16. Hugli T. E., Müller-Eberhard H. J. Anaphylatoxins: C3a and C5a. Adv Immunol. 1978;26:1–53. doi: 10.1016/s0065-2776(08)60228-x. [DOI] [PubMed] [Google Scholar]
  17. Löfgren S., Tärnvik A., Carlsson J. Demonstration of opsonizing antibodies to Francisella tularensis by leukocyte chemiluminescence. Infect Immun. 1980 Aug;29(2):329–334. doi: 10.1128/iai.29.2.329-334.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. McPhail L. C., DeChatelet L. R., Johnston R. B., Jr Generation of chemiluminescence by a particulate fraction isolated from human neutrophils. Analysis of molecular events. J Clin Invest. 1979 Apr;63(4):648–655. doi: 10.1172/JCI109347. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. NILSSON U. R., MUELLER-EBERHARD H. J. ISOLATION OF BETA IF-GLOBULIN FROM HUMAN SERUM AND ITS CHARACTERIZATION AS THE FIFTH COMPONENT OF COMPLEMENT. J Exp Med. 1965 Aug 1;122:277–298. doi: 10.1084/jem.122.2.277. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Rosen H., Klebanoff S. J. Chemiluminescence and superoxide production by myeloperoxidase-deficient leukocytes. J Clin Invest. 1976 Jul;58(1):50–60. doi: 10.1172/JCI108458. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. SKOOG W. A., BECK W. S. Studies on the fibrinogen, dextran and phytohemagglutinin methods of isolating leukocytes. Blood. 1956 May;11(5):436–454. [PubMed] [Google Scholar]
  22. Schiffmann E., Corcoran B. A., Wahl S. M. N-formylmethionyl peptides as chemoattractants for leucocytes. Proc Natl Acad Sci U S A. 1975 Mar;72(3):1059–1062. doi: 10.1073/pnas.72.3.1059. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Stjernholm R. L., Allen R. C., Steele R. H., Waring W. W., Harris J. A. Impaired chemiluminescence during phagocytosis of opsonized bacteria. Infect Immun. 1973 Feb;7(2):313–314. doi: 10.1128/iai.7.2.313-314.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Webb L. S., Keele B. B., Jr, Johnston R. B., Jr Inhibition of phagocytosis-associated chemiluminescence by superoxide dismutase. Infect Immun. 1974 Jun;9(6):1051–1056. doi: 10.1128/iai.9.6.1051-1056.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES