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. 1980 Aug;29(2):361–368. doi: 10.1128/iai.29.2.361-368.1980

Serotypes of Enterotoxigenic Escherichia coli Isolated in the United States

John M DeBoy II 1,2, I Kaye Wachsmuth 2, Betty R Davis 2
PMCID: PMC551126  PMID: 7011973

Abstract

Strains of enterotoxigenic Escherichia coli isolated from humans in the United States were found in 11 of 16 serotypes that previously were documented in the international literature as associated with enterotoxin production. Of 68 strains belonging to these 11 serotypes, 28 (41%) were enterotoxigenic; none of 46 strains belonging to 5 other previously implicated serotypes was enterotoxigenic. Control cultures of various serotypes were selected for comparison and found to contain 0 to 7% enterotoxigenic E. coli. E. coli belonging to documented enterotoxin-associated serotypes, characterized by both O and H antigens, were selected for toxin testing to determine their prevalence and potential pathogenicity in this country. In this study, a strain possessing any combination of an enterotoxin-associated serotype O antigen and H antigen was more likely to be enterotoxigenic than strains possessing only the specific O antigen or H antigen or neither. Five E. coli strains belonging to undocumented enterotoxin-associated serotypes did contain a combination of previously reported enterotoxin-associated serotype O and H antigens and did produce enterotoxin.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brunton J., Hinde D., Langston C., Gross R., Rowe B., Gurwith M. Enterotoxigenic Escherichia coli in central Canada. J Clin Microbiol. 1980 Apr;11(4):343–348. doi: 10.1128/jcm.11.4.343-348.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Dean A. G., Ching Y. C., Williams R. G., Harden L. B. Test for Escherichia coli enterotoxin using infant mice: application in a study of diarrhea in children in Honolulu. J Infect Dis. 1972 Apr;125(4):407–411. doi: 10.1093/infdis/125.4.407. [DOI] [PubMed] [Google Scholar]
  3. Donta S. T., Moon H. W., Whipp S. C. Detection of heat-labile Escherichia coli enterotoxin with the use of adrenal cells in tissue culture. Science. 1974 Jan 25;183(4122):334–336. doi: 10.1126/science.183.4122.334. [DOI] [PubMed] [Google Scholar]
  4. Evans D. J., Jr, Evans D. G., DuPont H. L., Orskov F., Orskov I. Patterns of loss of enterotoxigenicity by Escherichia coli isolated from adults with diarrhea: suggestive evidence for an interrelationship with serotype. Infect Immun. 1977 Jul;17(1):105–111. doi: 10.1128/iai.17.1.105-111.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Evans D. J., Jr, Evans D. G., Gorbach S. L. Production of vascular permeability factor by enterotoxigenic Escherichia coli isolated from man. Infect Immun. 1973 Nov;8(5):725–730. doi: 10.1128/iai.8.5.725-730.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Evans D. J., Jr, Evans D. G. Three characteristics associated with enterotoxigenic Escherichia coli isolated from man. Infect Immun. 1973 Sep;8(3):322–328. doi: 10.1128/iai.8.3.322-328.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Giannella R. A. Suckling mouse model for detection of heat-stable Escherichia coli enterotoxin: characteristics of the model. Infect Immun. 1976 Jul;14(1):95–99. doi: 10.1128/iai.14.1.95-99.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Guerrant R. L., Brunton L. L., Schnaitman T. C., Rebhun L. I., Gilman A. G. Cyclic adenosine monophosphate and alteration of Chinese hamster ovary cell morphology: a rapid, sensitive in vitro assay for the enterotoxins of Vibrio cholerae and Escherichia coli. Infect Immun. 1974 Aug;10(2):320–327. doi: 10.1128/iai.10.2.320-327.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Gyles C., So M., Falkow S. The enterotoxin plasmids of Escherichia coli. J Infect Dis. 1974 Jul;130(1):40–49. doi: 10.1093/infdis/130.1.40. [DOI] [PubMed] [Google Scholar]
  10. Merson M. H., Orskov F., Orskov I., Sack R. B., Huq I., Koster F. T. Relationship between enterotoxin production and serotype in enterotoxigenic Escherichia coli. Infect Immun. 1979 Feb;23(2):325–329. doi: 10.1128/iai.23.2.325-329.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Moon H. W., Fung P. Y., Whipp S. C., Isaacson R. E. Effects of age and ambient temperature on the responses of infant mice to heat-stable enterotoxin of Escherichia coli: assay modifications. Infect Immun. 1978 Apr;20(1):36–39. doi: 10.1128/iai.20.1.36-39.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Orskov F., Orskov I., Evans D. J., Jr, Sack R. B., Sack D. A., Wadström T. Special Escherichia coli serotypes among enterotoxigenic strains from diarrhoea in adults and children. Med Microbiol Immunol. 1976 Jun 1;162(2):73–80. doi: 10.1007/BF02121318. [DOI] [PubMed] [Google Scholar]
  13. Orskov I., Orskov F. Special O:K:H serotypes among enterotoxigenic E. coli strains from diarrhea in adults and children. Occurrence of the CF (colonization factor) antigen and of hemagglutinating abilities. Med Microbiol Immunol. 1977 Jul 18;163(2):99–110. doi: 10.1007/BF02121825. [DOI] [PubMed] [Google Scholar]
  14. Rowe B., Scotland S. M., Gross R. J. Enterotoxigenic Escherichia coli causing infantile enteritis in Britain. Lancet. 1977 Jan 8;1(8002):90–91. doi: 10.1016/s0140-6736(77)91099-6. [DOI] [PubMed] [Google Scholar]
  15. Sack D. A., Sack R. B. Test for enterotoxigenic Escherichia coli using Y-1 adrenal cells in miniculture. Infect Immun. 1975 Feb;11(2):334–336. doi: 10.1128/iai.11.2.334-336.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Sack R. B., Sack D. A., Mehlman I. J., Orskov F., Orskov I. Enterotoxigenic Escherichia coli isolated from food. J Infect Dis. 1977 Feb;135(2):313–317. doi: 10.1093/infdis/135.2.313. [DOI] [PubMed] [Google Scholar]
  17. Wachsmuth I. K., Stamm W. E., McGowan J. E., Jr Prevalence of toxigenic and invasive strains of Escherichia coli in a hospital population. J Infect Dis. 1975 Nov;132(5):601–603. doi: 10.1093/infdis/132.5.601. [DOI] [PubMed] [Google Scholar]

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