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. 1980 Sep;29(3):853–858. doi: 10.1128/iai.29.3.853-858.1980

Candida albicans group A-specific soluble antigens demonstrated by quantitative immunoelectrophoresis.

R M Guinet, S M Gabriel
PMCID: PMC551208  PMID: 6159327

Abstract

Soluble cytoplasmic extracts of Candida albicans groups A and B were prepared and compared by quantitative immunoelectrophoresis experiments performed with a commercial anti-C. albicans group A immune serum. Although crossed immunoelectrophoresis, tandem crossed immunoelectrophoresis, and line immunoelectrophoresis revealed many cross-reactions between the two groups, some components seemed to be specific to group A. However, the complexity of the extracts studied did not allow us to demonstrate specific constituents with these methods. Crossed-line immunoelectrophoresis with and without absorption of antibodies in situ was then used, and four specific antigens unique to group A cytoplasmic extract were demonstrated, one of which appeared to be quantitatively important. The value of various quantitative immunoelectrophoretic methods applied to complex antigenic preparations is discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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