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. 1980 Sep;29(3):934–939. doi: 10.1128/iai.29.3.934-939.1980

Galactose-containing polysaccharides from the human pathogens Sporothrix schenckii and Ceratocystis stenoceras.

L Mendonça-Previato, P A Gorin, L R Travassos
PMCID: PMC551220  PMID: 7429638

Abstract

Galactose-containing polysaccharides from three strains of Sporothrix schenckii and one strain of Ceratocystis stenoceras were isolated, and their structures were paritally characterized by chemical analysis and 13C-nuclear magnetic resonance spectroscopy (13C-NMR). S. schenckii polysaccharide preparations from all strains were not precipitated by Fehling solution and contained galactomannan (or a mixture of galactan and galactomannan), amylose, and minor amounts of rhamnomannan. C. stenoceras polysaccharide contained galactomannan and a smaller proportion of amylose. Conventional chemical techniques and 13C-NMR spectroscopy showed that the structures of the two preparations were closely related. The core of the galactomannan consisted principally of nonreducing end units and 2-O-, 2,6-di-O-, and perhaps 2,3-di-O-substituted alpha-D-mannopyranosyl units. The core was substituted by beta-D-galactofuranosyl chains; the units are interlinked (1 leads to 6). 13C-NMR evidence shows that the alpha-D-mannopyranosyl units are substituted in the two positions by the beta-D-galactofuranosyl residues. Galactomannans present at the cell surface of S. schenckii represent other potential fungal antigens in addition to the already recognized rhamnomannans and their peptide complexes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Gorin P. A., Haskins R. H., Travassos L. R., Mendonca-Previato L. Further studies on the rhamnomannans and acidic rhamnomannans of Sporothrix schenckii and Ceratocystis stenoceras. Carbohydr Res. 1977 May;55:21–33. doi: 10.1016/s0008-6215(00)84440-7. [DOI] [PubMed] [Google Scholar]
  2. Haworth W. N., Raistrick H., Stacey M. Polysaccharides synthesised by micro-organisms: The molecular structure of mannocarolose produced from glucose by Penicillium Charlesii G. Smith. Biochem J. 1935 Mar;29(3):612–621. doi: 10.1042/bj0290612. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Ishizaki H., Nakamura Y., Kariya H., Iwatsu T., Wheat R. Delayed hypersensitivity cross-reactions between Sporothrix schenckii and Ceratocystis species in sporotrichotic patients. J Clin Microbiol. 1976 Jun;3(6):545–547. doi: 10.1128/jcm.3.6.545-547.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Ishizaki H., Nakamura Y., Kurata Y., Iwatsu T., Wheat R. W. Delayed hypersensitivity cross-reactions among Sporothrix species in sporotrichotic patients. J Dermatol. 1980 Feb;7(1):75–76. doi: 10.1111/j.1346-8138.1980.tb01946.x. [DOI] [PubMed] [Google Scholar]
  5. Ishizaki H., Wheat R. W., Kiel D. P., Conant N. F. Serological cross-reactivity among Sporothrix schenckii, Ceratocystis, Europhium, and Graphium species. Infect Immun. 1978 Aug;21(2):585–593. doi: 10.1128/iai.21.2.585-593.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Lloyd K. O., Bitoon M. A. Isolation and purification of a peptido-rhamnomannan from the yeast form of Sporothrix schenckii. Structural and immunochemical studies. J Immunol. 1971 Sep;107(3):663–671. [PubMed] [Google Scholar]
  7. Lloyd K. O., Travassos L. R. Immunochemical studies on L-rhamno-D-mannans of Sporothrix schenckii and related fungi by use of rabbit and human antisera. Carbohydr Res. 1975 Mar;40(1):89–97. doi: 10.1016/s0008-6215(00)82671-3. [DOI] [PubMed] [Google Scholar]
  8. Mariat F. Observations sur l'écologie de Sporothrix schenckii et de Ceratocystis stenoceras en Corse et en Alsace, Provinces francaises indemnes de sporotrichose. Sabouraudia. 1975 Jul;13(2):217–225. [PubMed] [Google Scholar]
  9. Mendonça L., Gorin P. A., Lloyd K. O., Travassos L. R. Polymorphism of Sporothrix schenckii surface polysaccharides as a function of morphological differentiation. Biochemistry. 1976 Jun 1;15(11):2423–2431. doi: 10.1021/bi00656a028. [DOI] [PubMed] [Google Scholar]
  10. Nakamura Y., Ishizaki H., Wheat R. W. Serological cross-reactivity between group B Streptococcus and Sporothrix schenckii, Ceratocystis species, and Graphium species. Infect Immun. 1977 May;16(2):547–549. doi: 10.1128/iai.16.2.547-549.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Suzuki S., Takeda N. Immunochemical studies on the galactomannans isolated from mycelia and culture broths of three Hormodendrum strains. Infect Immun. 1977 Sep;17(3):483–490. doi: 10.1128/iai.17.3.483-490.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Travassos L. R., Gorin P. A., Lloyd K. O. Comparison of the rhamnomannans from the human pathogen Sporothrix schenckii with those from the Ceratocystis species. Infect Immun. 1973 Nov;8(5):685–693. doi: 10.1128/iai.8.5.685-693.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Travassos L. R., Gorin P. A., Lloyd K. O. Discrimination between Sporothrix schenckii and Ceratocystis stenoceras rhamnomannans by proton and carbon-13 magnetic resonance spectroscopy. Infect Immun. 1974 Apr;9(4):674–680. doi: 10.1128/iai.9.4.674-680.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]

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