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. 1980 Nov;30(2):362–369. doi: 10.1128/iai.30.2.362-369.1980

Cytotoxic lymphocytes and antibody-dependent complement-mediated cytotoxicity induced by administration of influenza vaccine.

G V Quinnan, F A Ennis, C U Tuazon, M A Wells, G M Butchko, R Armstrong, C McLaren, J F Manischewitz, S Kiley
PMCID: PMC551319  PMID: 7439982

Abstract

Recently defined aspects of cellular and humoral antiviral immunity were evaluated in 10 young adults given influenza vaccines containing A/USSR/77 (H1N1) antigens. Cytotoxic lymphocytes were measured by using cryopreserved lymphocytes as effector cells and syngeneic, virus-infected lymphocytes as target cells. An assay previously developed in this laboratory was adapted for the measurement of antibody-dependent, complement-mediated cytotoxicity. Antiviral cytotoxic lymphocyte responses were detected in 5 of 10 volunteers between 3 and 10 days after the initial vaccination. These responses were found both in individuals who were previously primed and in individuals who were not primed to influenza A/USSR/77 antigens. The complement-mediated lysis assay was found to be more sensitive than the hemagglutination inhibition test and probably detected antibodies to both subtype-specific and cross-reactive antigenic determinants. These responses to influenza antigens are similar to those obtained in studies of murine influenza which indicate that cytotoxic lymphocytes and antibody-dependent, complement-mediated cytotoxicity have a role in the early response to acute infection.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Appleyard G., Maber H. B. Plaque formation by influenza viruses in the presence of trypsin. J Gen Virol. 1974 Dec;25(3):351–357. doi: 10.1099/0022-1317-25-3-351. [DOI] [PubMed] [Google Scholar]
  2. Butchko G. M., Armstrong R. B., Martin W. J., Ennis F. A. Influenza A viruses of the H2N2 subtype are lymphocyte mitogens. Nature. 1978 Jan 5;271(5640):66–67. doi: 10.1038/271066a0. [DOI] [PubMed] [Google Scholar]
  3. Dolin R., Murphy B. R., Caplan E. A. Lymphocyte blastogenic responses to influenza virus antigens after influenza infection and vaccination in humans. Infect Immun. 1978 Mar;19(3):867–874. doi: 10.1128/iai.19.3.867-874.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Effros R. B., Doherty P. C., Gerhard W., Bennink J. Generation of both cross-reactive and virus-specific T-cell populations after immunization with serologically distinct influenza A viruses. J Exp Med. 1977 Mar 1;145(3):557–568. doi: 10.1084/jem.145.3.557. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Ennis F. A., Martin W. J., Verbonitz M. W., Butchko G. M. Specificity studies on cytotoxic thymus-derived lymphocytes reactive with influenza virus-infected cells: evidence for dual recognition of H-2 and viral hemagglutinin antigens. Proc Natl Acad Sci U S A. 1977 Jul;74(7):3006–3010. doi: 10.1073/pnas.74.7.3006. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Ennis F. A., Martin W. J., Verbonitz M. W. Cytotoxic T lymphocytes induced in mice by inactivated influenza virus vaccine. Nature. 1977 Sep 29;269(5627):418–419. doi: 10.1038/269418a0. [DOI] [PubMed] [Google Scholar]
  7. Ennis F. A., Martin W. J., Verbonitz M. W. Hemagglutinin-specific cytotoxic T-cell response during influenza infection. J Exp Med. 1977 Sep 1;146(3):893–898. doi: 10.1084/jem.146.3.893. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Ennis F. A., Wells M. A., Butchko G. M., Albrecht P. Evidence that cytotoxic T cells are part of the host's response to influenza pneumonia. J Exp Med. 1978 Nov 1;148(5):1241–1250. doi: 10.1084/jem.148.5.1241. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Farrohi K., Farrohi F. K., Noble G. R., Kaye H. S., Kendal A. P. Evaluation of the single radial hemolysis test for measuring hemagglutinin- and neuraminidase-specific antibodies to H3N2 influenza strains and antibodies to influenza B. J Clin Microbiol. 1977 Mar;5(3):353–360. doi: 10.1128/jcm.5.3.353-360.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Galama J. M., Lucas C. J., Vos A. Lymphocyte-mediated cytotoxicity to cells infected with measles virus. I. Use of in vitro infected leukocytes as autologous target cells; absence of virus-specific cytotoxic lymphocytes. Cell Immunol. 1978 Jul;38(2):365–377. doi: 10.1016/0008-8749(78)90067-9. [DOI] [PubMed] [Google Scholar]
  11. Greenberg S. B., Criswell B. S., Six H. R., Couch R. B. Lymphocyte cytotoxicity to influenza virus-infected cells: response to vaccination and virus infection. Infect Immun. 1978 Jun;20(3):640–645. doi: 10.1128/iai.20.3.640-645.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Gregg M. B., Hinman A. R., Craven R. B. The Russian flu. Its history and implications for this year's influenza season. JAMA. 1978 Nov 17;240(21):2260–2263. doi: 10.1001/jama.240.21.2260. [DOI] [PubMed] [Google Scholar]
  13. HERS J. F., MULDER J. Broad aspects of the pathology and pathogenesis of human influenza. Am Rev Respir Dis. 1961 Feb;83(2):84–97. doi: 10.1164/arrd.1961.83.2P2.84. [DOI] [PubMed] [Google Scholar]
  14. Julius M. H., Simpson E., Herzenberg L. A. A rapid method for the isolation of functional thymus-derived murine lymphocytes. Eur J Immunol. 1973 Oct;3(10):645–649. doi: 10.1002/eji.1830031011. [DOI] [PubMed] [Google Scholar]
  15. Kaplan M. E., Clark C. An improved rosetting assay for detection of human T lymphocytes. J Immunol Methods. 1974 Jul;5(2):131–135. doi: 10.1016/0022-1759(74)90003-9. [DOI] [PubMed] [Google Scholar]
  16. McMichael A. J., Ting A., Zweerink H. J., Askonas B. A. HLA restriction of cell-mediated lysis of influenza virus-infected human cells. Nature. 1977 Dec 8;270(5637):524–526. doi: 10.1038/270524a0. [DOI] [PubMed] [Google Scholar]
  17. McMichael A. HLA restriction of human cytotoxic T lymphocytes specific for influenza virus. Poor recognition of virus associated with HLA A2. J Exp Med. 1978 Dec 1;148(6):1458–1467. doi: 10.1084/jem.148.6.1458. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Potter C. W., Jennings R., Nicholson K. Immunity to influenza virus infection induced by heterologous, inactivated vaccines. Med Microbiol Immunol. 1978 Nov 17;166(1-4):99–108. doi: 10.1007/BF02121139. [DOI] [PubMed] [Google Scholar]
  19. Quinnan G. V., Manischewitz J. E., Ennis F. A. Cytotoxic T lymphocyte response to murine cytomegalovirus infection. Nature. 1978 Jun 15;273(5663):541–543. doi: 10.1038/273541a0. [DOI] [PubMed] [Google Scholar]
  20. Roos D., Loos J. A. Changes in the carbohydrate metabolism of mitogenically stimulated human peripheral lymphocytes. I. Stimulation by phytohaemagglutinin. Biochim Biophys Acta. 1970 Dec 29;222(3):565–582. doi: 10.1016/0304-4165(70)90182-0. [DOI] [PubMed] [Google Scholar]
  21. Shaw S., Biddison W. E. HLA-linked genetic control of the specicity of human cytotoxic T-cell responses to influenza virus. J Exp Med. 1979 Mar 1;149(3):565–575. doi: 10.1084/jem.149.3.565. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Shore S. L., Cromeans T. L., Romano T. J. Immune destruction of virus-infected cells early in the infectious cycle. Nature. 1976 Aug 19;262(5570):695–696. doi: 10.1038/262695a0. [DOI] [PubMed] [Google Scholar]
  23. Tauraso N. M., Pedreira F. A., Spector S. L., Bernier G. M. Effect of various methods of removing non-specific inhibitors to virus hemagglutination upon serum proteins and immunoglobulins. Arch Gesamte Virusforsch. 1971;34(3):214–222. doi: 10.1007/BF01242995. [DOI] [PubMed] [Google Scholar]
  24. Verbonitz M. W., Ennis F. A., Hicks J. T., Albrecht P. Hemagglutinin-specific complement-dependent cytolytic antibody response to influenza infection. J Exp Med. 1978 Jan 1;147(1):265–270. doi: 10.1084/jem.147.1.265. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. van Oers M. H., Zeijlemaker W. P., Schellekens P. T. Separation and properties of EA-rosette-forming lymphocytes in humans. Eur J Immunol. 1977 Mar;7(3):143–150. doi: 10.1002/eji.1830070306. [DOI] [PubMed] [Google Scholar]

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