Skip to main content
NCBI home page
Infection and Immunity logoLink to Infection and Immunity
. 1980 Dec;30(3):766–772. doi: 10.1128/iai.30.3.766-772.1980

Immunochemical properties of antigen-specific monkey T-cell suppressor factor induced with a Streptococcus mutans antigen.

J R Lamb, E D Zanders, S Kontiainen, T Lehner
PMCID: PMC551381  PMID: 6164645

Abstract

Antigen-specific suppressor factor could be released from monkey suppressor T cells induced in vitro with a protein antigen isolated from the carcinogenic bacterium Streptococcus mutans. The suppressor activity was due to the factor itself and not to carryover of free antigen. Characterization of the monkey factor revealed it to have a molecular weight of ca. 70,000, and to contain a constant region and determinants encoded by the major histocompatibility complex. The presence of immunoglobulin determinants could not be demonstrated. However, by virtue of its adsorption to specific antigen, an antigen-combining site was shown to be present. The possible regulatory role of streptococcal antigen-specific suppressor factor in protection against dental caries is discussed.

Full text

PDF
769

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brodsky F. M., Parham P., Barnstable C. J., Crumpton M. J., Bodmer W. F. Monoclonal antibodies for analysis of the HLA system. Immunol Rev. 1979;47:3–61. doi: 10.1111/j.1600-065x.1979.tb00288.x. [DOI] [PubMed] [Google Scholar]
  2. Cunningham A. J., Szenberg A. Further improvements in the plaque technique for detecting single antibody-forming cells. Immunology. 1968 Apr;14(4):599–600. [PMC free article] [PubMed] [Google Scholar]
  3. Germain R. N., Ju S. T., Kipps T. J., Benacerraf B., Dorf M. E. Shared idiotypic determinants on antibodies and T-cell-derived suppressor factor specific for the random terpolymer L-glutamic acid60-L-alanine30-L-tyrosine10. J Exp Med. 1979 Mar 1;149(3):613–622. doi: 10.1084/jem.149.3.613. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Germain R. N., Thèze J., Kapp J. A., Benacerraf B. Antigen-specific T-cell-mediated suppression. I. Induction of L-glutamic acid60-L-alanine30-L-tyrosine10 specific suppressor T cells in vitro requires both antigen-specific T-cell-suppressor factor and antigen. J Exp Med. 1978 Jan 1;147(1):123–136. doi: 10.1084/jem.147.1.123. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. HUNTER W. M., GREENWOOD F. C. Preparation of iodine-131 labelled human growth hormone of high specific activity. Nature. 1962 May 5;194:495–496. doi: 10.1038/194495a0. [DOI] [PubMed] [Google Scholar]
  6. Kontiainen S., Feldmann M. Conditions for inducing T helper cells in vitro. Scand J Immunol. 1975;4(2):121–128. doi: 10.1111/j.1365-3083.1975.tb02608.x. [DOI] [PubMed] [Google Scholar]
  7. Kontiainen S., Feldmann M. Structural characteristics of antigen-specific suppressor factors: definition of 'constant' region and 'variable' region determinants. Thymus. 1979 Sep;1(1-2):59–79. [PubMed] [Google Scholar]
  8. Kontiainen S., Feldmann M. Suppressor-cell induction in vitro. IV. Target of antigen-specific suppressor factor and its genetic relationships. J Exp Med. 1978 Jan 1;147(1):110–122. doi: 10.1084/jem.147.1.110. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kontiainen S., Simpson E., Bohrer E., Beverley P. C., Herzenberg L. A., Fitzpatrick W. C., Vogt P., Torano A., McKenzie I. F., Feldmann M. T-cell lines producing antigen-specific suppressor factor. Nature. 1978 Aug 3;274(5670):477–480. doi: 10.1038/274477a0. [DOI] [PubMed] [Google Scholar]
  10. Kontianen S., Feldmann M. Induction of specific helper cells in vitro. Nat New Biol. 1973 Oct 31;245(148):285–286. doi: 10.1038/newbio245285a0. [DOI] [PubMed] [Google Scholar]
  11. Kontianen S., Howie S., Maurer P. H., Feldmann M. Suppressor cell induction in vitro. VI. Production of suppressor factors to synthetic polypeptides GAT and (T,G)-A--L from cells of responder and nonresponder mice. J Immunol. 1979 Jan;122(1):253–259. [PubMed] [Google Scholar]
  12. Kontianinen S., Feldmann M. Suppressor cell induction in vitro. III. Antigen-specific suppression by supernatants of suppressor cells. Eur J Immunol. 1977 May;7(5):310–314. doi: 10.1002/eji.1830070514. [DOI] [PubMed] [Google Scholar]
  13. Lamb J. R., Kontiainen S., Lehner T. Generation of specfic T-cell suppressor function induced by Streptococcus mutans in monkeys and mice. Infect Immun. 1979 Dec;26(3):903–909. doi: 10.1128/iai.26.3.903-909.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Lehner T., Challacombe S. J., Caldwell J. Immunologic basis for vaccination against dental caries in rhesus monkeys. J Dent Res. 1976 Apr;55(Spec No):C166–C180. doi: 10.1177/002203457605500311011. [DOI] [PubMed] [Google Scholar]
  15. Lehner T., Russell M. W., Wilton J. M., Challacombe S. J., Scully C. M., Hawkes J. E. Passive immunization with antisera to Streptococcus mutans in the prevention of caries in rhesus monkeys. Adv Exp Med Biol. 1978;107:303–315. doi: 10.1007/978-1-4684-3369-2_35. [DOI] [PubMed] [Google Scholar]
  16. Merler E., Gatien J., DeWilde G. Significance of immunofluorescent staining of lymphocytes with antisera to IgM immunoglobulins. Nature. 1974 Oct 18;251(5476):652–654. doi: 10.1038/251652a0. [DOI] [PubMed] [Google Scholar]
  17. Russell M. W., Bergmeier L. A., Zanders E. D., Lehner T. Protein antigens of Streptococcus mutans: purification and properties of a double antigen and its protease-resistant component. Infect Immun. 1980 May;28(2):486–493. doi: 10.1128/iai.28.2.486-493.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Russell M. W., Challacombe S. J., Lehner T. Specificity of antibodies induced by Streptococcus mutans during immunization against dental caries. Immunology. 1980 May;40(1):97–106. [PMC free article] [PubMed] [Google Scholar]
  19. Russell M. W., Lehner T. Characterisation of antigens extracted from cells and culture fluids of Streptococcus mutans serotype c. Arch Oral Biol. 1978;23(1):7–15. doi: 10.1016/0003-9969(78)90047-x. [DOI] [PubMed] [Google Scholar]
  20. Tada T., Taniguchi M., David C. S. Suppressive and enhancing T-cell factors as I-region gene products: properties and the subregion assignment. Cold Spring Harb Symp Quant Biol. 1977;41(Pt 1):119–127. doi: 10.1101/sqb.1977.041.01.016. [DOI] [PubMed] [Google Scholar]
  21. Tada T., Taniguchi M., Takemori T. Properties of primed suppressor T cells and their products. Transplant Rev. 1975;26:106–129. doi: 10.1111/j.1600-065x.1975.tb00177.x. [DOI] [PubMed] [Google Scholar]
  22. Takemori T., Tada T. Properties of antigen-specific suppressive T-cell factor in the regulation of antibody response of the mouse. I. In vivo activity and immunochemical characterization. J Exp Med. 1975 Nov 1;142(5):1241–1253. doi: 10.1084/jem.142.5.1241. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Taniguchi M., Miller J. F. Specific suppression of the immune response by a factor obtained from spleen cells of mice tolerant to human gamma-globulin. J Immunol. 1978 Jan;120(1):21–26. [PubMed] [Google Scholar]
  24. Taniguchi M., Saito T., Tada T. Antigen-specific suppressive factor produced by a transplantable I-J bearing T-cell hybridoma. Nature. 1979 Apr 5;278(5704):555–558. doi: 10.1038/278555a0. [DOI] [PubMed] [Google Scholar]
  25. Tanzer J. M., Hageage G. J., Jr, Larson R. H. Variable experiences in immunization of rats against Streptococcus mutans-associated dental caries. Arch Oral Biol. 1973 Nov;18(11):1425–1439. doi: 10.1016/0003-9969(73)90117-9. [DOI] [PubMed] [Google Scholar]
  26. Taussig M. J., Holliman A. Structure of an antigen-specific suppressor factor produced by a hybrid T-cell line. Nature. 1979 Jan 25;277(5694):308–310. doi: 10.1038/277308a0. [DOI] [PubMed] [Google Scholar]
  27. Theze J., Kapp J. A., Benacerraf B. Immunosuppressive factor(s) extracted from lymphoid cells of nonresponder mice primed with L-glutamic acid60-L-alanine30-L-tyrosine10 (GAT) III. Immunochemical properties of the GAT-specific suppressive factor. J Exp Med. 1977 Apr 1;145(4):839–856. doi: 10.1084/jem.145.4.839. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES