Skip to main content
PMC home page
Infection and Immunity logoLink to Infection and Immunity
. 1982 Jun;36(3):851–856. doi: 10.1128/iai.36.3.851-856.1982

Serotypes of enterotoxigenic Escherichia coli in Thailand and the Philippines.

P Echeverria, F Orskov, I Orskov, D Plianbangchang
PMCID: PMC551407  PMID: 7047398

Abstract

The serotypes of 386 enterotoxigenic Escherichia coli (ETEC) isolated from 82 individuals with and without diarrhea in Thailand and the Philippines were determined. The 136 strains producing both heat-labile toxin (LT) and heat-stable toxin (ST) belonged to 12 different O serogroups; however, 83% (113/136) were of one of four serogroups (O6, O8, O25, and O78), and 76% of (104/136) belonged to one of seven O:K:H serotypes. Only 14% (28/196) of LT-only-producing ETEC belonged to serogroups most common among LT and ST strains, and these 196 strains belonged to 35 different O:K:H serotypes. Three O serogroups (O20, O27, and O78) accounted for 94% (52/54) of strains producing only ST. Although only 4% (2/54) of ST-only ETEC belonged to the seven serotypes most commonly found among strains which produced LT and ST, 85% of ETEC belonged to three other serotypes, O20:K?:H21, O27:K?:H7, and O78:H-. A total of 46% (37/80) of ETEC of serotypes O6:H16, O8:H9, O25:H42, and O78:H12 were resistant to two or more antibiotics in comparison to 68% (208/306) of ETEC of other serotypes (P less than 0.001). In Thailand and the Philippines, E. coli which produced LT and ST or ST alone, but not those which produced LT alone, were restricted in their O:K:H serotypes.

Full text

PDF
854

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bauer A. W., Kirby W. M., Sherris J. C., Turck M. Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol. 1966 Apr;45(4):493–496. [PubMed] [Google Scholar]
  2. Echeverria P., Blacklow N. R., Sanford L. B., Cukor G. G. Travelers' diarrhea among American Peace Corps volunteers in rural Thailand. J Infect Dis. 1981 Jun;143(6):767–771. doi: 10.1093/infdis/143.6.767. [DOI] [PubMed] [Google Scholar]
  3. Echeverria P., Blacklow N. R., Vollet J. L., 3rd, Ulyangco C. V., Cukor G., Soriano V. B., DuPont H. L., Cross J. H., Orskov F., Orskov I. Reovirus-like agent and enterotoxigenic Escherichia coli infections in pediatric diarrhea in the Philippines. J Infect Dis. 1978 Sep;138(3):326–332. doi: 10.1093/infdis/138.3.326. [DOI] [PubMed] [Google Scholar]
  4. Echeverria P., Blacklow N. R., Zipkin C., Vollet J. J., Olson J. A., DuPont H. L., Cross J. H. Etiology of gastroenteritis among Americans living in the Philippines. Am J Epidemiol. 1979 Apr;109(4):493–501. doi: 10.1093/oxfordjournals.aje.a112706. [DOI] [PubMed] [Google Scholar]
  5. Echeverria P., Cross J. H. Heat-labile enterotoxigenic Escherichia coli and intestinal protozoa in asymptomatic travellers. Southeast Asian J Trop Med Public Health. 1977 Dec;8(4):476–479. [PubMed] [Google Scholar]
  6. Echeverria P., Mejia P. A., Duangmani C. Effect of antibiotics on the prevalence of enterotoxigenic Escherichia coli in two populations in the Philippines. Antimicrob Agents Chemother. 1981 Feb;19(2):290–293. doi: 10.1128/aac.19.2.290. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Evans D. G., Olarte J., DuPont H. L., Evans D. J., Jr, Galindo E., Portnoy B. L., Conklin R. H. Enteropathogens associated with pediatric diarrhea in Mexico City. J Pediatr. 1977 Jul;91(1):65–68. doi: 10.1016/s0022-3476(77)80446-0. [DOI] [PubMed] [Google Scholar]
  8. Evans D. J., Jr, Evans D. G., DuPont H. L., Orskov F., Orskov I. Patterns of loss of enterotoxigenicity by Escherichia coli isolated from adults with diarrhea: suggestive evidence for an interrelationship with serotype. Infect Immun. 1977 Jul;17(1):105–111. doi: 10.1128/iai.17.1.105-111.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Gyles C., So M., Falkow S. The enterotoxin plasmids of Escherichia coli. J Infect Dis. 1974 Jul;130(1):40–49. doi: 10.1093/infdis/130.1.40. [DOI] [PubMed] [Google Scholar]
  10. Leksomboon U., Echeverria P., Suvongse C., Duangmani C. Viruses and bacteria in pediatric diarrhea in Thailand: a study of multiple antibiotic-resistant enteric pathogens. Am J Trop Med Hyg. 1981 Nov;30(6):1281–1290. doi: 10.4269/ajtmh.1981.30.1281. [DOI] [PubMed] [Google Scholar]
  11. Merson M. H., Morris G. K., Sack D. A., Wells J. G., Feeley J. C., Sack R. B., Creech W. B., Kapikian A. Z., Gangarosa E. J. Travelers' diarrhea in Mexico. A prospective study of physicians and family members attending a congress. N Engl J Med. 1976 Jun 10;294(24):1299–1305. doi: 10.1056/NEJM197606102942401. [DOI] [PubMed] [Google Scholar]
  12. Merson M. H., Orskov F., Orskov I., Sack R. B., Huq I., Koster F. T. Relationship between enterotoxin production and serotype in enterotoxigenic Escherichia coli. Infect Immun. 1979 Feb;23(2):325–329. doi: 10.1128/iai.23.2.325-329.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Merson M. H., Rowe B., Black R. E., Huq I., Gross R. J., Eusof A. Use of antisera for identification of enterotoxigenic Escherichia coli. Lancet. 1980 Aug 2;2(8188):222–224. doi: 10.1016/s0140-6736(80)90118-x. [DOI] [PubMed] [Google Scholar]
  14. Morris G. K., Merson M. H., Sack D. A., Wells J. G., Martin W. T., Dewitt W. E., Feeley J. C., Sack R. B., Bessudo D. M. Laboratory investigation of diarrhea in travelers to Mexico: evaluation of methods for detecting enterotoxigenic Echerichia coli. J Clin Microbiol. 1976 May;3(5):486–495. doi: 10.1128/jcm.3.5.486-495.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Orskov F., Orskov I. Escherichia coli O:H serotypes isolated from human blood. Prevalence of the K1 antigen with technical details of O and H antigenic determination. Acta Pathol Microbiol Scand Suppl. 1975 Dec;83(6):595–600. [PubMed] [Google Scholar]
  16. Orskov F., Orskov I., Evans D. J., Jr, Sack R. B., Sack D. A., Wadström T. Special Escherichia coli serotypes among enterotoxigenic strains from diarrhoea in adults and children. Med Microbiol Immunol. 1976 Jun 1;162(2):73–80. doi: 10.1007/BF02121318. [DOI] [PubMed] [Google Scholar]
  17. Rowe B., Scotland S. M., Gross R. J. Enterotoxigenic Escherichia coli causing infantile enteritis in Britain. Lancet. 1977 Jan 8;1(8002):90–91. doi: 10.1016/s0140-6736(77)91099-6. [DOI] [PubMed] [Google Scholar]
  18. Ryder R. W., Sack D. A., Kapikian A. Z., McLaughlin J. C., Chakraborty J., Mizanur Rahman A. S., Merson M. H., Wells J. G. Enterotoxigenic Escherichia coli and Reovirus-like agent in rural Bangladesh. Lancet. 1976 Mar 27;1(7961):659–663. doi: 10.1016/s0140-6736(76)92776-8. [DOI] [PubMed] [Google Scholar]
  19. Sack D. A., McLaughlin J. C., Sack R. B., Orskov F., Orskov I. Enterotoxigenic Escherichia coli isolated from patients at a hospital in Dacca. J Infect Dis. 1977 Feb;135(2):275–280. doi: 10.1093/infdis/135.2.275. [DOI] [PubMed] [Google Scholar]
  20. Sack D. A., Sack R. B. Test for enterotoxigenic Escherichia coli using Y-1 adrenal cells in miniculture. Infect Immun. 1975 Feb;11(2):334–336. doi: 10.1128/iai.11.2.334-336.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Sack R. B., Gorbach S. L., Banwell J. G., Jacobs B., Chatterjee B. D., Mitra R. C. Enterotoxigenic Escherichia coli isolated from patients with severe cholera-like disease. J Infect Dis. 1971 Apr;123(4):378–385. doi: 10.1093/infdis/123.4.378. [DOI] [PubMed] [Google Scholar]
  22. Semjen G., Orskov I., Orskov F. K antigen determination of Escherichia coli by counter-current immunoelectrophoresis (CIE). Acta Pathol Microbiol Scand B. 1977 Feb;85B(1):103–107. doi: 10.1111/j.1699-0463.1977.tb01680.x. [DOI] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES