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. 1990 Nov;9(11):3667–3673. doi: 10.1002/j.1460-2075.1990.tb07578.x

The nematode spliced leader RNA participates in trans-splicing as an Sm snRNP.

P A Maroney 1, G J Hannon 1, J A Denker 1, T W Nilsen 1
PMCID: PMC552121  PMID: 2145151

Abstract

The trans-spliced leader RNA (SL RNA) of nematodes resembles U snRNAs both in cap structure and in the presence of a consensus Sm binding site. We show here that synthetic SL RNA, synthesized by in vitro transcription, is efficiently used as a spliced leader donor in trans-splicing reactions catalyzed by a cell free extract prepared from developing embryos of the parasitic nematode, Ascaris lumbricoides. Efficient utilization of synthetic SL RNA requires a functional Sm binding site. Mutations within the Sm binding sequence that prevent immunoprecipitation by Sm antisera and prevent cap trimethylation abolish trans-splicing. The effect on trans-splicing is not due to undermethylation of the cap structure.

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