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. 1982;1(5):603–608. doi: 10.1002/j.1460-2075.1982.tb01215.x

Regulated high efficiency expression of human interferon-alpha in Saccharomyces cerevisiae.

M F Tuite, M J Dobson, N A Roberts, R M King, D C Burke, S M Kingsman, A J Kingsman
PMCID: PMC553094  PMID: 6329694

Abstract

The 5' control region of the yeast phosphoglycerate kinase gene (PGK) was fused to the coding sequence of a human interferon-alpha. This PGK-interferon fusion was then introduced into yeast on a high copy number 2mu-based plasmid vector. Strains containing this plasmid produced a PGK-interferon-alpha fusion protein as 1-2% of cell protein and the expression of interferon activity was regulated by the availability of a fermentable carbon source. The system is capable of making as much as 15 mg of human interferon-alpha per litre of batch culture.

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Selected References

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