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. 1987 Mar;6(3):561–565. doi: 10.1002/j.1460-2075.1987.tb04791.x

The GABAA/benzodiazepine receptor is a heterotetramer of homologous alpha and beta subunits.

C Mamalaki, F A Stephenson, E A Barnard
PMCID: PMC553434  PMID: 3034594

Abstract

The GABAA receptor has been purified to homogeneity from bovine cerebral cortex. Under stringent conditions of isolation, the GABAA receptor was shown to consist only of alpha (Mr 53 000) and beta (Mr 57 000) subunits. A densitometric scan of SDS-PAGE gels under reducing conditions showed that these subunits were present in a 1:1 ratio. A model of the receptor as a heterologous tetramer alpha 2 beta 2 is proposed. Monoclonal antibodies have been raised to the purified bovine GABAA receptor. One of these antibodies, 1A6, was shown to react with both the alpha and beta subunits of the purified receptor. The subunits were still positive in immunoblots following the removal of the carbohydrate moieties of the respective polypeptides by endoglycosidase F treatment. This antibody has been employed to demonstrate antigenic cross-reactivity between the GABAA receptors of three vertebrate species. It is further proposed that there is partial amino acid sequence homology between the alpha and beta polypeptides and hence that they are derived from a single ancestral gene.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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