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. 1987 Sep;6(9):2531–2535. doi: 10.1002/j.1460-2075.1987.tb02540.x

Metal ion regulated gene expression: use of a plastocyanin-less mutant of Chlamydomonas reinhardtii to study the Cu(II)-dependent expression of cytochrome c-552.

S Merchant 1, L Bogorad 1
PMCID: PMC553670  PMID: 2824187

Abstract

Ac-208, a plastocyanin-deficient strain of Chlamydomonas reinhardtii, lacks the mRNA for pre-apoplastocyanin although Southern analyses indicate no gross deletion in the single gene for pre-apoplastocyanin. The alternative electron carrier, cytochrome c-552, is expressed only in Cu(II)-deficient cells of the mutant. Thus, the mutant strain can grow photoautotrophically in Cu(II)-deficient but not in Cu(II)-sufficient medium. This indicates that the absence of plastocyanin is the only defect (with respect to photosynthesis) in ac-208. Phenotypic revertants of ac-208 are capable of photoautotrophic growth by virtue of constitutive [with respect to Cu(II)] synthesis of cytochrome c-552. The revertant phenotype is attributed to a decreased internal concentration of Cu(II). This indicates that the Cu(II)-dependent expression of cytochrome c-552 synthesis is affected directly by the concentration of Cu(II) rather than indirectly by the level of plastocyanin in the cell.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bishop P. E., Jarlenski D. M., Hetherington D. R. Expression of an alternative nitrogen fixation system in Azotobacter vinelandii. J Bacteriol. 1982 Jun;150(3):1244–1251. doi: 10.1128/jb.150.3.1244-1251.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bohner H., Sandmann G., Böger P. Formation of silver plastocyanin in Scenedesmus. Biochim Biophys Acta. 1981 Jun 12;636(1):65–69. doi: 10.1016/0005-2728(81)90076-1. [DOI] [PubMed] [Google Scholar]
  3. Daldal F., Cheng S., Applebaum J., Davidson E., Prince R. C. Cytochrome c(2) is not essential for photosynthetic growth of Rhodopseudomonas capsulata. Proc Natl Acad Sci U S A. 1986 Apr;83(7):2012–2016. doi: 10.1073/pnas.83.7.2012. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dickerson R. E. Cytochrome c and the evolution of energy metabolism. Sci Am. 1980 Mar;242(3):137–153. [PubMed] [Google Scholar]
  5. Garrett T. P., Clingeleffer D. J., Guss J. M., Rogers S. J., Freeman H. C. The crystal structure of poplar apoplastocyanin at 1.8-A resolution. The geometry of the copper-binding site is created by the polypeptide. J Biol Chem. 1984 Mar 10;259(5):2822–2825. doi: 10.2210/pdb2pcy/pdb. [DOI] [PubMed] [Google Scholar]
  6. Gorman D. S., Levine R. P. Cytochrome f and plastocyanin: their sequence in the photosynthetic electron transport chain of Chlamydomonas reinhardi. Proc Natl Acad Sci U S A. 1965 Dec;54(6):1665–1669. doi: 10.1073/pnas.54.6.1665. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gorman D. S., Levine R. P. Photosynthetic Electron Transport Chain of Chlamydomonas reinhardi VI. Electron Transport in Mutant Strains Lacking Either Cytochrome 553 or Plastocyanin. Plant Physiol. 1966 Dec;41(10):1648–1656. doi: 10.1104/pp.41.10.1648. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Keller L. R., Schloss J. A., Silflow C. D., Rosenbaum J. L. Transcription of alpha- and beta-tubulin genes in vitro in isolated Chlamydomonas reinhardi nuclei. J Cell Biol. 1984 Mar;98(3):1138–1143. doi: 10.1083/jcb.98.3.1138. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. LEVINE R. P. A screening technique for photosynthetic mutants in unicellular algae. Nature. 1960 Oct 22;188:339–340. doi: 10.1038/188339b0. [DOI] [PubMed] [Google Scholar]
  10. Merchant S., Bogorad L. Rapid degradation of apoplastocyanin in Cu(II)-deficient cells of Chlamydomonas reinhardtii. J Biol Chem. 1986 Dec 5;261(34):15850–15853. [PubMed] [Google Scholar]
  11. Merchant S., Bogorad L. Regulation by copper of the expression of plastocyanin and cytochrome c552 in Chlamydomonas reinhardi. Mol Cell Biol. 1986 Feb;6(2):462–469. doi: 10.1128/mcb.6.2.462. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Merchant S., Bogorad L. The Cu(II)-repressible plastidic cytochrome c. Cloning and sequence of a complementary DNA for the pre-apoprotein. J Biol Chem. 1987 Jul 5;262(19):9062–9067. [PubMed] [Google Scholar]
  13. Myers A. M., Grant D. M., Rabert D. K., Harris E. H., Boynton J. E., Gillham N. W. Mutants of Chlamydomonas reinhardtii with physical alterations in their chloroplast DNA. Plasmid. 1982 Mar;7(2):133–151. doi: 10.1016/0147-619x(82)90073-7. [DOI] [PubMed] [Google Scholar]
  14. SAGER R., GRANICK S. Nutritional studies with Chlamydomonas reinhardi. Ann N Y Acad Sci. 1953 Oct 14;56(5):831–838. doi: 10.1111/j.1749-6632.1953.tb30261.x. [DOI] [PubMed] [Google Scholar]
  15. Sandmann G., Malkin R. Iron-sulfur centers and activities of the photosynthetic electron transport chain in iron-deficient cultures of the blue-green alga aphanocapsa. Plant Physiol. 1983 Nov;73(3):724–728. doi: 10.1104/pp.73.3.724. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Spreitzer R. J., Mets L. Photosynthesis-deficient Mutants of Chlamydomonas reinhardii with Associated Light-sensitive Phenotypes. Plant Physiol. 1981 Mar;67(3):565–569. doi: 10.1104/pp.67.3.565. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Sueoka N. MITOTIC REPLICATION OF DEOXYRIBONUCLEIC ACID IN CHLAMYDOMONAS REINHARDI. Proc Natl Acad Sci U S A. 1960 Jan;46(1):83–91. doi: 10.1073/pnas.46.1.83. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Wood P. M. Interchangeable copper and iron proteins in algal photosynthesis. Studies on plastocyanin and cytochrome c-552 in Chlamydomonas. Eur J Biochem. 1978 Jun 1;87(1):9–19. doi: 10.1111/j.1432-1033.1978.tb12346.x. [DOI] [PubMed] [Google Scholar]

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