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. 1987 Dec 1;6(12):3565–3569. doi: 10.1002/j.1460-2075.1987.tb02686.x

5' Analysis of the soybean leghaemoglobin lbc3 gene: regulatory elements required for promoter activity and organ specificity

Jens Stougaard 1, Niels N Sandal 1, Anette Grøn 1, Astrid Kühle 1, Kjeld A Marcker 1
PMCID: PMC553822  PMID: 16453808

Abstract

The soybean leghaemoglobin lbc3 gene promoter was analysed in transgenic Lotus corniculatus plants. Hybrid-promoter constructions and 5' deletions were studied using chimeric genes composed of the various promoters, the chloramphenicol acetyltransferase (CAT) coding sequence and the lbc3 3' flanking region. A 5' Bal31 deletion series mapped a strong positive regulatory element between −1100 and −950. A weaker element located between −230 and −170 defined the minimum 5' region required for detectable promoter activity. Reactivation of inactive promoters with deletion endpoints between −230 and the transcription initiation site was obtained employing the constitutive cauliflower mosaic virus (CaMV) 35S enhancer. The position of cis regulatory element(s) required for nodule-specific expression was defined to 37 bp between −139 and −102. This region contains sequences conserved in other leghaemoglobin and nodulin genes. No indispensable control elements were found on the lbc3 3' flanking region.

Keywords: promoter elements, Bal31 deletions, hybrid promoters, root nodule expression, transgenic legumes

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Selected References

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