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. 1985 Jul;4(7):1631–1635. doi: 10.1002/j.1460-2075.1985.tb03830.x

Hormonal regulation of β1,3-glucanase messenger RNA levels in cultured tobacco tissues

Debra Mohnen 1, Hideaki Shinshi 1, Georg Felix 1, Frederick Meins Jr 1
PMCID: PMC554397  PMID: 16453619

Abstract

We describe the isolation of a cDNA clone of β1,3-glucanase mRNA from Nicotiana tabacum L. cv. `Havana 425' and its use to measure the kinetics of mRNA accumulation in cultured tobacco tissues treated with the plant hormones auxin and cytokinin. Northern blot analysis showed that the tissues contain a single ˜1.6 kb-sized β1,3-glucanase mRNA. The levels of β1,3-glucanase and β1,3-glucanase mRNA increase by up to seven- and 20-fold, respectively, over a 7-day period in tissues subcultured on hormone-free medium and medium containing auxin or cytokinin added separately. Over the same interval of time, the content of both the enzyme and its mRNA remains at a constant low level in tissues subcultured on medium containing both auxin and cytokinin. The results show that auxin and cytokinin block β1,3-glucanase production at the level of the mRNA.

Keywords: auxin; cytokinin; β1,3-glucanase; cDNA clone; Nicotiana tabacum (tissue culture)

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