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. 1983;2(3):339–344. doi: 10.1002/j.1460-2075.1983.tb01428.x

Adenovirus cores can function as templates in in vitro DNA replication.

C R Goding 1, W C Russell 1
PMCID: PMC555138  PMID: 11894947

Abstract

Adenovirus cores prepared by gentle disruption of virus by heating at 56 degrees C in the presence of deoxycholate were able to function as templates in an in vitro DNA replication system, allowing both initiation, indicated by the formation of terminal protein-dCMP complex, and elongation of > 300 nucleotides. Using both cores and DNA-protein complexes as templates, it was also demonstrated that novobiocin, an inhibitor of DNA gyrase, inhibited in vitro DNA replication by preventing formation of the initiation complex.

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Selected References

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