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. 1983;2(6):927–934. doi: 10.1002/j.1460-2075.1983.tb01523.x

Microdissection and cloning of the white locus and the 3B1-3C2 region of the Drosophila X chromosome

V Pirrotta 1,*, C Hadfield 1,1, GHJ Pretorius 1,2
PMCID: PMC555210  PMID: 16453459

Abstract

Fragments from the 3B-3C region of the Drosophila X chromosomes were microdissected from salivary gland squashes and their DNA was cloned by the method developed by Scalenghe et al. (1981). These clones were used as starting points for a chromosome walk which covers 200 kb including bands 3B2 to 3C2. A number of deletion breakpoints were mapped on the cloned DNA allowing the localisation of several genes in the 3B region. The white locus in particular was isolated by microcloning the wa insertion site. Two transcripts, of 2 and 2.4 kb, respectively, arise from the white region and its vicinity.

Keywords: Drosophila, microcloning, repetitive sequences, white locus

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