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. 1983;2(10):1747–1751. doi: 10.1002/j.1460-2075.1983.tb01652.x

Heat-shock puff 93 D from Drosophila melanogaster: accumulation of a RNP-specific antigen associated with giant particles of possible storage function.

A Dangli, C Grond, P Kloetzel, E K Bautz
PMCID: PMC555353  PMID: 6416825

Abstract

The monoclonal antibody P11 is directed against a 38 000 dalton protein of Drosophila melanogaster. On polytene chromosomes this protein is present in a subset of the RNA polymerase II-containing loci. Here we show by density centrifugation and enzyme-linked immunosorbent assay tests that the P11 antigen is part of nuclear ribonucleoprotein (RNP) complexes. Indirect immunofluorescence shows that, after prolonged heat-shock, the P11 antigen is present only in the heat-shock puff 93 D. Identical distribution patterns were obtained with another monoclonal antibody, Q18. Unlike P11, this antibody also cross-reacts with D. hydei and D. virilis polytene chromosomes, where the puffs 48 B and 20 CD, respectively, are the only loci prominently stained after heat-shock. The small and giant RNP complexes previously described in these puffs were also observed in puff 93 D. Both types of particle contain the P11 antigen as shown by immunoelectron microscopy. We suggest that the P11 antigen is associated with a special class of RNPs which are possibly involved in the storage of primary transcription products inside the nucleus.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ashburner M., Bonner J. J. The induction of gene activity in drosophilia by heat shock. Cell. 1979 Jun;17(2):241–254. doi: 10.1016/0092-8674(79)90150-8. [DOI] [PubMed] [Google Scholar]
  2. Bonner J. J., Pardue M. L. The effect of heat shock on RNA synthesis in Drosophila tissues. Cell. 1976 May;8(1):43–50. doi: 10.1016/0092-8674(76)90183-5. [DOI] [PubMed] [Google Scholar]
  3. Derksen J., Berendes H. D., Willart E. Production and release of a locus-specific ribonucleoprotein product in polytene nuclei of Drosophila hydei. J Cell Biol. 1973 Dec;59(3):661–668. doi: 10.1083/jcb.59.3.661. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Derksen J. Cytological analysis of Drosophila polytene. Methods Cell Biol. 1978;17:133–140. doi: 10.1016/s0091-679x(08)61141-3. [DOI] [PubMed] [Google Scholar]
  5. Derksen J., Willart E. Cytochemical studies on RNP complexes produced by puff 2-48BC in Drosophila hydei: uranyl acetate and phosphotungstic acid staining. Chromosoma. 1976 Mar 31;55(1):57–68. doi: 10.1007/BF00288327. [DOI] [PubMed] [Google Scholar]
  6. Echalier G., Ohanessian A. In vitro culture of Drosophila melanogaster embryonic cells. In Vitro. 1970 Nov-Dec;6(3):162–172. doi: 10.1007/BF02617759. [DOI] [PubMed] [Google Scholar]
  7. Grond C. J., Lubsen N. H., Beck H. Recombination frequency and DNA content of the distal part of the second chromosome of Drosophila hydei Sturtevant. Experientia. 1982 Mar 15;38(3):328–329. doi: 10.1007/BF01949372. [DOI] [PubMed] [Google Scholar]
  8. Jamrich M., Greenleaf A. L., Bautz E. K. Localization of RNA polymerase in polytene chromosomes of Drosophila melanogaster. Proc Natl Acad Sci U S A. 1977 May;74(5):2079–2083. doi: 10.1073/pnas.74.5.2079. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kabisch R., Bautz E. K. Differential distribution of RNA polymerase B and nonhistone chromosomal proteins in polytene chromosomes of Drosophila melanogaster. EMBO J. 1983;2(3):395–402. doi: 10.1002/j.1460-2075.1983.tb01436.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kloetzel P. M., Johnson R. M., Sommerville J. Interaction of the hnRNA of amphibian oocytes with fibril-forming proteins. Eur J Biochem. 1982 Oct;127(2):301–308. doi: 10.1111/j.1432-1033.1982.tb06870.x. [DOI] [PubMed] [Google Scholar]
  11. Lakhotia S. C., Mukherjee T. Specific activation of puff 93D of Drosophila melanogaster by benzamide and the effect of benzamide treatment on the heat shock induced puffing activity. Chromosoma. 1980;81(1):125–136. doi: 10.1007/BF00292427. [DOI] [PubMed] [Google Scholar]
  12. Lengyel J. A., Ransom L. J., Graham M. L., Pardue M. L. Transcription and metabolism of RNA from the Drosophila melanogaster heat shock puff site 93D. Chromosoma. 1980;80(3):237–252. doi: 10.1007/BF00292683. [DOI] [PubMed] [Google Scholar]
  13. Lengyel J., Penman S. hnRNA size and processing as related to different DNA content in two dipterans: Drosophila and Aedes. Cell. 1975 Jul;5(3):281–290. doi: 10.1016/0092-8674(75)90103-8. [DOI] [PubMed] [Google Scholar]
  14. Lubsen N. H., Sondermeijer P. J., Pages M., Alonso C. In situ hybridization of nuclear and cytoplasmic RNA to locus 2-48BC in Drosophila hydei. Chromosoma. 1978 Feb 10;65(3):199–212. doi: 10.1007/BF00327617. [DOI] [PubMed] [Google Scholar]
  15. Mohler J., Pardue M. L. Deficiency mapping of the 93D heat-shock locus in Drosophila melanogaster. Chromosoma. 1982;86(4):457–467. doi: 10.1007/BF00330121. [DOI] [PubMed] [Google Scholar]
  16. Peters F. P., Grond C. J., Sondermeijer P. J., Lubsen N. H. Chromosomal arrangement of heat shock locus 2-48B in Drosophila hydei. Chromosoma. 1982;85(2):237–249. doi: 10.1007/BF00294968. [DOI] [PubMed] [Google Scholar]
  17. Peters F. P., Lubsen N. H., Sondermeijer P. J. Rapid sequence divergence in a heat shock locus of Drosophila. Chromosoma. 1980;81(2):271–280. doi: 10.1007/BF00285953. [DOI] [PubMed] [Google Scholar]
  18. Saumweber H., Symmons P., Kabisch R., Will H., Bonhoeffer F. Monoclonal antibodies against chromosomal proteins of Drosophila melanogaster: establishment of antibody producing cell lines and partial characterization of corresponding antigens. Chromosoma. 1980;80(3):253–275. doi: 10.1007/BF00292684. [DOI] [PubMed] [Google Scholar]

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