Update on the effects of physical activity on insulin sensitivity in humans

Stephen R Bird; John A Hawley

doi:10.1136/bmjsem-2016-000143

. 2017 Mar 1;2(1):e000143. doi: 10.1136/bmjsem-2016-000143

Update on the effects of physical activity on insulin sensitivity in humans

Stephen R Bird ^1,^{Correspondence to}, John A Hawley ^2,³

PMCID: PMC5569266 PMID: 28879026

Abstract

Purpose and methods

This review presents established knowledge on the effects of physical activity (PA) on whole-body insulin sensitivity (SI) and summarises the findings of recent (2013–2016) studies.

Discussion and conclusions

Recent studies provide further evidence to support the notion that regular PA reduces the risk of insulin resistance, metabolic syndrome and type 2 diabetes, and SI improves when individuals comply with exercise and/or PA guidelines. Many studies indicate a dose response, with higher energy expenditures and higher exercise intensities, including high intensity interval training (HIIT), producing greater benefits on whole-body SI, although these findings are not unanimous. Aerobic exercise interventions can improve SI without an associated increase in cardiorespiratory fitness as measured by maximal or peak oxygen consumption. Both aerobic and resistance exercise can induce improvements in glycaemic regulation, with some suggestions that exercise regimens including both may be more efficacious than either exercise mode alone. Some studies report exercise-induced benefits to SI that are independent of habitual diet and weight loss, while others indicate an association with fat reduction, hence the debate over the relative importance of PA and weight loss continues. During exercise, muscle contraction stimulated improvements in SI are associated with increases in AMPK activity, which deactivates TCB1D1, promoting GLUT4 translocation to the cell membrane and thereby increasing glucose uptake. Postexercise, increases in Akt deactivate TCB1D4 and thereby increase GLUT4 translocation to the cell membrane. The reduction in intramuscular saturated fatty acids and concomitant reductions in ceramides, but not diacylglycerols, provide a potential link between intramuscular lipid content and SI. Increased skeletal muscle capillarisation provides another independent adaptation through which SI is improved, as does enhanced β cell activity. Recent studies are combining exercise interventions with dietary and feeding manipulations to investigate the potential for augmenting the exercise-induced improvements in SI and glycaemic control.

Keywords: Physical Activity, Insulin Sensitivity (SI), Diabetes

Key messages.

What are the new findings?

In addition to adding further support to the established position that a lifestyle that includes regular physical activity (PA) is associated with a good insulin sensitivity (SI) and exercise interventions can improve SI, evidence is growing for the following key findings:

Aerobic exercise may increase SI without a measurable increase in VO_{2 max} or VO_{2 peak}.
A dose effect may be evident, with greater exercise volumes and higher exercise intensities, including high intensity interval training (HIIT) or sprint interval training (SIT), producing greater benefits to SI.
The combination of aerobic exercise training and REX may be more effective than either exercise mode alone.
Exercise induced benefits may be augmented by appropriate dietary and feeding manipulations.
Molecular research has identified key signalling molecules and proteins that are influenced by exercise and provide the link to resultant changes in SI.
Evidence is accumulating for ceramides to be the causal link between obesity and a reduced SI.

Practical recommendations

Despite the aforementioned general consensus, not all findings are consistent, and the specific details of the most efficacious forms of exercise/physical activity (PA) for improving or maintaining insulin sensitivity (SI) require further elucidation in order for exercise prescription to be optimised.
Research needs to assess the interaction of dietary/feeding manipulations and exercise on SI and glycaemic control, as these may augment the beneficial outcomes of the interventions.
Future research needs to consider the potential influence of exercise induced improvements to β cell function and increased muscle capillarisation, alongside the contribution of intramuscular changes that result in improved SI, GLUT4 availability and glycaemic control.
Studies will also need to consider potential differences in the adaptations induced by different exercise modalities, aerobic versus REX; exercise intensities and volumes, including interval training (high intensity interval training (HIIT) and sprint interval training (SIT)); and differences in the adaptations of different fibre types.
Likewise, the potential to adapt and improve SI is likely to be influenced by the basal state of the participants: with healthy participants, overweight/obese, prediabetic metabolic syndrome, and patients with diabetes all likely to differ in the magnitude of adaptation and improvement.
Given the evident benefits of PA/exercise interventions for preventing diabetes, even among those with metabolic risk factors, studies aimed at identifying effective preventive strategies are paramount in order to prevent further increases in the prevalence of type 2 diabetes, particularly since only 10% of current clinical trials focus on prevention and only ~12% use behavioural interventions such as PA rather than drugs, which are the focus of ~63.1% of studies.¹⁰¹

Introduction

Individuals with poor insulin sensitivity (SI) are characterised by impaired insulin action on whole-body glucose uptake. This results in elevated blood (glucose), impaired glycaemic control, a risk of pancreatic β cell failure and the development of type 2 diabetes (T2D). In developed countries the prevalence of this prediabetic state is currently reported to be 15–20%.¹ Furthermore, it is estimated that 366 million people, ~8% of the population are affected by diabetes worldwide,² hence strategies for the treatment of the prediabetic state, its prevention and preventing progression from prediabetes to T2D are an imperative. Key among these is the inclusion of physical activity (PA) into a healthy lifestyle, and current research in this field continues to seek to understand the behavioural and molecular aspects of exercise in preventing diabetes and poor SI, with the intent to identify efficacious exercise interventions.

The comparison of results between research studies into the effects of a physically active lifestyle and/or exercise on insulin sensitivity and glycaemic control are problematic due to differences in the methods of assessment of outcome variables. While the precise protocols vary, the general methods for assessing insulin sensitivity/glycaemic control include: (1) measuring fasting insulin concentrations, with elevated fasted (insulin) >25 mIU/L indicating poor insulin sensitivity, as the pancreas endeavours to compensate for the lack of peripheral insulin sensitivity by secreting greater amounts of insulin, thereby resulting in hyperinsulinaemia; (2) oral glucose tolerance testing (OGTT), which involves the ingestion of a standard glucose bolus (75 g), followed by blood glucose monitoring for the subsequent 2 hours. Blood glucose concentrations of >7.8 mmol/L and <11.0 mmol/L at 2 hours are indicative of impaired glycaemic control, and >11.0 mmol/L indicates diabetes; (3) hyperinsulinaemic-euglycaemic clamp, in which the participant is infused with insulin at a known rate, creating a hyperinsulinaemic state (~100 µU/ml), while simultaneously blood glucose levels are monitored and adjusted by a variable-rate infusion to maintain glycaemia (5.0–5.5 mmol/L). A high rate of glucose infusion indicates insulin sensitivity as the glucose is being rapidly taken up by the cells of the body, while a low rate of glucose infusion indicates a loss of insulin sensitivity, as the glucose is remaining in the blood rather than being taken up by the cells of the body;³ (4) hyperglycaemic clamp, in which plasma glucose levels are initially increased to ~125mg/dl above basal values and then maintained at this hyperglycaemic level, through the infusion of glucose. High infusion rates indicate good insulin sensitivity, while low infusion rates indicate insulin resistance (IR).³ ; (5) homoeostatic model assessment of insulin resistance (HOMA-IR), which uses fasting (glucose) and insulin, and is calculated as (glucose mmol/L x insulin)/22.5, with a relatively low score indicating well regulated fasting glucose that is being maintained through relatively low concentrations of insulin, hence good insulin sensitivity, whereas an elevated HOMA-IR value, such as >2.5 indicates IR. In the updated homoeostatic model, HOMA2-IR, values >1.5 suggest IR; (6) HOMA-β is a measure of β cell function derived from fasting values using the equation (20 x insulin)/(glucose mmol/L−3.5) %, with this measure indicating the extent to which a deficient β cell function, as opposed to IR, contributes to hyperglycaemia in the fasting state; (7) Quantitative Insulin Sensitivity Check Index (QUICKI), which is an index of insulin sensitivity, calculated as QUICKI=1/(log (fasting plasma insulin µU/ml) + log (fasting blood glucose mg/dL)).⁴

Regardless of the methods used to assess insulin sensitivity/glycaemic control, a lifestyle incorporating regular PA has been identified as a key factor for maintaining and improving many aspects of health, including insulin sensitivity.^{5 6} In this context, the term PA covers all forms of muscular movement, including that associated with strenuous physical work, active transport (walking and cycling), household tasks (cleaning and gardening), incidental PA which occurs when undertaking other tasks, sport and other active leisure pursuits. Whereas the term ‘exercise’ refers specifically to the context of PA that is undertaken with the specific intent of improving health and/or fitness and is therefore a subset of PA. Hence many cross-sectional studies investigate PA levels as well as specific exercise habits, but interventions tend to involve exercise, as they have the specific intent of affecting an aspect of health.

Cross-sectional studies identify an association between regular PA and/or aerobic fitness and superior SI.^{5 6} Adding further support to this association, studies involving exercise interventions usually report an amelioration or in some cases, complete reversal of IR.^{7 8} Assessments of the impact of a physically active lifestyle suggest a dose response with each 500 kcal/week increase in PA, reducing the risk of T2D by ~9%.⁹

Physical activity has both immediate (acute) and longer-term effects on insulin sensitivity. The immediate effects are the direct result of a single exercise bout and may be evident during and/or for up to 72 hours postexercise. If repeated regularly these bouts produce additional long-term chronic improvements to SI, thereby providing superior baseline glycaemic control compared with that typically seen in less active individuals. In this healthy, physically active, ‘trained’ condition, the effects of individual exercise bouts may then produce further acute responses from this already elevated SI state and thereby promote optimal SI and glycaemic control. Some key issues around PA that are considered in recent literature include: the effects of manipulating the mode of exercise; the influence of exercise intensity and exercise duration; the potential benefits of high intensity interval training (HIIT); and the relative effects of the aforementioned on groups of different ages and at different levels of impaired SI. Other innovative strategies that have received recent attention include assessing whether the impact of exercise on SI is affected by whether it is undertaken in a fed or fasted state, and whether a short exercise bout (exercise snack) performed before meals is beneficial.

The purpose of this review is to provide an overview of the topic for those new to it and an update of recent developments for the established researcher.

Methods

A literature search was undertaken using PubMed in November 2015, using search terms ‘Exercise’ OR ‘Physical activity’, AND 'Insulin sensitivity’. This resulted in 10 185 articles, which were then limited to clinical trials (n=1672 articles), filtered using the terms ‘Human’ and limited to English language publications (n=1371). Reviews and key articles published in English since 2000 were used to present established knowledge and set the background context, while to identify recent updates the search was reduced to articles published after 2012: this resulted in 394 articles. The abstracts of these articles were then evaluated and studies included if the main focus was an exercise intervention or cross-sectional study of PA habits and not confounded by the inclusion of other interventions, such as drugs or diseases such as cancer. Studies were excluded if SI or other measures of glycaemic control were not included as an outcome measure. Titles were manually sorted and articles rejected if primary objectives were not exercise-based. They were then divided on the basis of whether they assessed the acute responses that occur during or immediately after a bout of PA, or the chronic adaptations that occur over a more prolonged period of time due to repeated exercise bouts—the training effects. A further search using the same search criteria was undertaken in July 2016 when the manuscript was undergoing minor revisions. This identified a further 80 articles that met the criteria of the search terms and the aforementioned manual sorting produced the resultant total of 53 recent articles which are summarised in tables 1 - 3. tables 1 and 3.

Table 1.

Summary of recent studies assessing acute SI responses to exercise

Reference	Participants	Study type	Exercise type and intensity	Outcome measure	Authors' conclusions and comments
de Matos et al²²	Twenty-seven obese patients or obese patients with insulin resistance.	Exercise intervention.	Acute 60 min of aerobic exercise on a cycle ergometer at 60% of peak oxygen consumption.	Compared with paired eutrophic controls, obese subjects had higher basal levels of p-JNK and p-IRS-1(ser612), and reduced HSP70. Exercise reduced p-IRS-1(ser612) for both obese and obese insulin-resistant subjects. A main effect of exercise was observed for HSP70.	A single session of exercise promotes changes that are characteristic of a reduction in cellular stress. Such changes may contribute to an exercise-induced increase in SI.
Rynders et al³⁸	Eighteen adults with prediabetes.	Randomised controlled trial of acute responses to exercise.	Moderate intensity exercise at LT versus high intensity exercise (75% of the difference between LT and peak O₂ consumption versus control (1 hour of seated rest). One hour after exercise, subjects undertook an OGTT.	SI improved by 51% following moderate intensity exercise and 85% following high-intensity exercise.	Acute exercise had an immediate and intensity-dependent effect on improving postprandial glycaemia and SI.
Newsom et al³⁹	Eleven sedentary, obese adults.	Randomised controlled trial.	Three experimental trials: (1) exercise at 50% VO_{2 peak}for~70 min (expending ~350 Kcal); (2) exercise at 65% VO_{2 peak}for ~55 min to expend 350 kcal; (3) no exercise. Exercise was undertaken in the afternoon and SI assessed the following morning.	Seventy minutes of exercise at 50% VO_{2 peak} increased insulin sensitivity by 35% compared with control condition. Whereas the 55 min of exercise at 65% VO_{2 peak} produced average increase in SI of 20% compared with control condition, this was not statistically significant.	A prolonged single session of exercise at a moderate intensity improved SI the next day in obese adults. This may be more effective than a shorter duration bout at a higher intensity.
Malin et al⁴⁰	Fifteen patients with prediabetes aged 49.9±3.6 years.	Randomised, controlled, cross-over trial, with control condition.	Three trial conditions: (1) 1 hour rest (control); (2) 200 kcal cycle ergometer exercise bout at LT; and (3) 200 kcal cycle ergometer exercise bout at 75% of difference between lactate threshold and VO_{2 peak}. A 75 g OGTT was undertaken 1 hour postexercise/control.	Compared with control, exercise lowered skeletal muscle IR independently of exercise intensity, but hepatic and adipose IR was increased. Glucose-stimulated insulin secretion did not differ between conditions, but postprandial glucose levels were lower postexercise.	Exercise promoted insulin sensitivity in skeletal muscle post exercise. The increase in IR in adipose and hepatic tissue may further promote glucose uptake and glycogen restoration in the muscles.
Ortega et al⁴³	Ten healthy young men.	Randomised cross-over trial with control condition.	SIT of 4 × 30 s sprints versus continuous low intensity exercise at 46% VO_{2 peak} versus moderate intensity exercise at 77% VO_{2 peak} versus control. Intravenous glucose tolerance tests undertaken 30 min, 24 hours and 48 hours postexercise.	All exercise conditions improved SI for at least 48 hours compared with the control condition. Thirty minutes postexercise the improvements induced by SIT were greater than for either of the continuous exercise bouts.	All exercise bouts improved SI, and in the short term (30 min postexercise) SIT was more effective than low or moderate intensity continuous exercise at improving SI.
Terada et al⁴⁴	Ten patients with diabetes aged 45–75 years.	Randomised, controlled, cross-over trial, with control condition.	Four exercise conditions each of 60 min duration: (1) HIIT (repetitions of 3 min at 40% VO_{2 peak} and x 1 min at 100% VO_{2 peak}) in fasted state; (2) HIIT postbreakfast; (3) Moderate intensity exercise (55% of VO_{2 peak}) in fasted state; and (4) moderate intensity exercise, postbreakfast; plus no exercise (control).	HIIT reduced overnight and fasting glycaemia the day after the exercise by more than moderate intensity exercise. Exercising in a fasted state rather than ‘post-breakfast’ attenuated postprandial glycaemic increments. Compared with the control condition, HIIT in a fasted state produced significant improvements to: 24-hour mean glucose, fasting glucose, postprandial glycaemic increment, glycaemic variability and time spent in hyperglycaemia.	HIIT resulted in acute benefits to glycaemic regulation, which were further enhanced by undertaking the exercise in a fasted state.
Whyte et al⁴⁵	Ten overweight/obese men aged 26.9±6.2 years.	Randomised, controlled, cross-over trial.	Three trial conditions: (1) four maximal 30 s sprints, with 4.5 min recovery between each (SIT); (2) a single maximal ES matched with SIT for work done; and (3) no exercise (CON). Oral glucose tolerance tests were undertaken on the days following each of the above.	SI Index was 44.6% higher following ES than CON, but did not differ significantly between SIT and CON. On the day following exercise, fat oxidation in the fasted state was increased by 63% and 38%, compared with CON, in SIT and ES, respectively.	A single ES, which may represent a more time-efficient alternative to SIT, can increase SI and increase fat oxidation in overweight/obese sedentary men.

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ES, extended sprint; HIIT, high intensity interval training; LT, lactate threshold; OGTT, oral glucose tolerance test; SI, insulin sensitivity; SIT, sprint interval training.

Table 3.

Summary of recent studies assessing exercise training effects on insulin sensitivity

Reference	Participants	Study type	Exercise mode	Outcome measure	Authors' conclusions and comments
Stuart et al¹⁵	Eleven participants with MetS and seven non-diabetic, sedentary controls.	Exercise intervention. Preintervention versus postintervention comparison.	Eight weeks of increasing intensity stationary cycle training.	Cycle training without weight loss did not change IR in MetS subjects or sedentary controls. Muscle insulin receptor expression increased in both MetS and sedentary groups, while GLUT4 expression also increased in the MetS subjects. The excess phosphorylation of IRS-1 at Ser337 in MetS muscle tended to increase further after training in spite of a decrease in total IRS-1.	In the absence of weight loss, the cycle training of MetS subjects increased the expression of insulin receptors and GLUT4 in muscle but did not decrease the IR.
Malin et al¹⁸	Twenty-four older, obese adults with IFG and/or IGT.	Exercise intervention.	Twelve weeks of exercise (60 min/day, 5 days/week at ∼85% HR_max).	Exercise increased clamp-derived peripheral and hepatic SI more in adults with IFG or IGT alone than with IFG + IGT.	Exercise increased peripheral but not hepatic SI.
Reichkendler et al¹⁹	Sixty-one, healthy, sedentary, moderately overweight young men.	Randomised, controlled trial.	Moderate (300 kcal/day) or high (600 kcal/day) physical exercise for 11 weeks, versus sedentary living (control). Pretraining and post-training, insulin-stimulated glucose uptake was assessed in five individual femoral muscle groups and four different adipose tissue regions.	Aerobic exercise training increased insulin-stimulated glucose uptake in skeletal muscle but not in adipose tissue.	Aerobic exercise training enhances glucose uptake in muscle but not adipose tissues, indicating a differential effect on these tissues.
Prior et al³²	Sixteen sedentary overweight, obese, older men and women, with IGT	Preintervention versus postintervention comparison.	Six months of aerobic exercise and weight loss. Three sessions a week progressing from 20 min at 50% HRR to 45 min at 85% of HRR.	Hyperinsulinaemic-euglycaemic clamp and OGTT. Capillary density was measured via biopsies of the vastus lateralis.	Insulin sensitivity increased and 120 min postprandial glucose was lower postintervention. These changes were associated with increases in capillary density.
Malin et al³⁴	Thirty-five older obese adults with prediabetes.	Exercise intervention.	Progressive 12-week exercise intervention (60 min at~85% HR_max 5 days/week).	Exercise increased first-phase and second-phase DI; β cell function = glucose-stimulated insulin secretion × clamp-derived SI).	Exercise training plus weight loss increased pancreatic β cell function in a linear dose-response manner in adults with prediabetes. Relatively high exercise doses (>2000 kcal/week) may be necessary to enhance β cell function in adults with poor insulin secretion capacity.
Madsen et al³⁶	Ten, non-active patients with T2D(56±2 years) and 13 matched healthy participants.	Preintervention versus postintervention comparison.	Three sessions per week of HIIT (10 × 60 s) for 8 weeks for both patients with T2D and healthy participants. Glycaemic control was assessed using HOMA-IR and β cell function assessed.	Patients with T2D displayed significant improvements in HOMA-IR and β cell function. The healthy group, who displayed superior HOMA-IR and β cell function results at baseline, exhibited no change in these measures, which was not unexpected given their baseline values.	HIIT was effective in improving HOMA-IR and β cell function in patients with T2D.
Brennan et al³⁵	Seventy-seven sedentary, obese men and women.	Repeated measures, intervention versus control condition.	Three to four months of aerobic exercise versus control	Hyperinsulinaemic-euglycaemic clamp and VO_{2 peak}.	Changes to insulin sensitivity were not associated with changes to VO_{2 peak}.
de Sousa et al⁹⁶	Forty-four patients with T2D, aged 48–68 years (27 women, 17 men).	Randomised trial: diet versus football training plus diet.	Football training: 3 × 40 min/week for 12 weeks.	Football training plus diet group displayed improvements in HOMA-IR, whereas diet alone did not.	Football training plus diet was potentially better at preventing T2D complications than diet alone. It was also more effective than diet alone at improving other markers of metabolic and cardiovascular health, such as blood lipid profile and CRF.
Motahari-Tabari, et al⁵²	Fifty-three women with T2D.	Randomised clinical trial: exercise versus control.	Thirty minutes at a maximum intensity of 60% increase in heart rate, three times a week for 8 weeks.	HOMA-IR improved and fasting plasma glucose and insulin were lowered.	Exercise was effective at improving SI.
Ryan et al⁵³	Seventy-seven overweight and obese, sedentary, postmenopausal women.	Prospective controlled study.	Six months of: ‘aerobic exercise (3 days/week) + weight loss’ versus ‘weight loss without exercise’.	IR decreased in both groups. Glucose utilisation increased by 10% with ‘aerobic exercise + weight loss’ and 8% with ‘weight loss without exercise’.	No statistically significant difference in changes to IR between ‘aerobic exercise + weight loss’ versus ‘weight loss without exercise’. However, exercise benefited other markers of metabolic health.
Mitranun et al⁵⁴	Forty-three participants with T2D.	Randomised controlled trial.	Sedentary (control) versus continuous exercise versus INT. For 30 min/day and 40 min/day, three times/week for 12 weeks.	Fasting blood glucose levels decreased in both exercise groups. Glycosylated haemoglobin levels decreased only in the interval trainingINT group.	Both continuous training and INT were effective in improving glycaemic control, but the INT programme appears to confer greater improvements.
Skleryk et al⁵⁵	Sixteen sedentary, obese men.	Exercise intervention.	Two weeks of reduced-volume SIT (three sessions of 8–12 × 10 s sprints/week) compared with TER (5 × 30 min sessions at 65% peak oxygen consumption/week).	HOMA-IR, AS160 phosphorylation and COX II, COX IV, GLUT-4, Nur77 and SIRT1 protein expression assessed at baseline and approximately 72 hours after the final training bout were unaltered in either group.	Two weeks of reduced-volume SIT or TER did not elicit any measurable metabolic adaptations in previously sedentary, obese men.
Trachta et al⁵⁶	Fifteen obese women.	Intervention with comparison group comprising ‘healthy’ lean subjects who did not undertake the exercise intervention.	Three-month exercise programme consisting of 30 min of aerobic exercise, three times a week.	HOMA-IR improved in the obese group.	Three months of regular exercise improved blood glucose and HOMA-IR, but had no significant effect on lipid profile and blood pressure.
Many et al⁵⁷	Eleven morbidly obese minority adolescents (BMI 41.4±1.8 kg/m²)	Exercise intervention.	Eight weeks of aerobic exercise training (~180 min/week at 40–55% VO_{2 peak}). Preintervention and postintervention, SI and inflammatory markers were assessed.	Insulin action improved in response to training, as indicated by a ~37% increase in SI.	This study supports the efficacy of exercise training interventions on improving MetSfeatures in morbidly obese minority youth.
Racil et al⁵⁸	Thirty-four obese adolescent girls.	Randomised controlled trial.	Twelve- weeks of MIIT or HIIT exercise.	Significant decrease in IR (HOMA-IR) occurred in both HIIT and MIIT groups (−29.2±5.3 and −18.4±8.6%, respectively.	INT improved SI. High intensity interval exercise produced greater benefits than moderate intensity interval exercise.
Kurose, et al⁵⁹	Forty three obese patients.	Exercise intervention.	Thirty minutes on a cycle ergometer or treadmill, three times per week for 6 months, with training intensity adjusted to anaerobic threshold.	HOMA-IR improved.	Aerobic exercise improved SI. Additionally, IR was the only independent factor influencing improvement in endothelial function.
Reichkendler et al⁶⁰	Sixty-one healthy sedentary moderately overweight young men.	Randomised controlled trial.	Eleven weeks of PA at moderate dose (300 kcal/day), high dose (600 kcal/day) or sedentary living.	In both exercise groups, peripheral SI improved. HOMO-IR decreased.	Physical activity improved SI and small additional health benefits were found when exercising at ∼3800 kcal/week versus ∼2000 kcal/week in young moderately overweight men.
Di Raimondo et al⁶¹	One hundred and seventy-six subjects with MetS.	Exercise intervention.	Walking for 1 hour, 5 days a week for 24 weeks at an intensity higher than the one classified as 'comfortable' by the patient.	Mean fasting glucose improved.	Regular walking at a moderate to hard intensity improved glycaemic control.
Lee et al⁶²	Forty-four obese adolescent girls.	Randomised controlled trial.	Three months of 180 min/week aerobic exercise versus resistance exercise versus a non-exercising control group. SI was evaluated by a 3-hour hyperinsulinamic (80 mU·m²/min) euglycaemic clamp.	Compared with control, aerobic exercise improved SI but resistance exercise did not.	In obese, adolescent girls, aerobic exercise but not resistance exercise was effective in improving SI and did so independently of weight loss or calorie restriction.
Bacchi et al⁶³	Thirty-one sedentary adults with T2D, and non-alcoholic fatty liver disease.	Randomised controlled trial.	Effects of 4 months of aerobic or resistance training on SI.	Post-training, SI was increased and hepatic fat content reduced in both groups.	Resistance training and AER were both effective in improving SI and reducing hepatic fat content in patients with non-alcoholic fatty liver disease.
Motahari-Tabari et al⁶⁴	Fifty-three women with T2D.	Exercise intervention versus non-exercise control condition.	Eight weeks of walking for 30 min three times a week.	Exercise improved HOMA-IR, fasting plasma insulin and glucose.	The exercise intervention was effective in lowering plasma glucose, insulin levels and IR.
Herzig et al⁶⁵	One hundred and thirteen men and women with prediabetes.	Exercise intervention versus non-exercise control condition.	Three sessions of 60 min walking per week, for 3 months versus non-exercise control.	The exercise intervention improved HOMA-IR, fasting insulin and glucose.	Compared with controls, the exercise group improved HOMA-IR and fasting insulin, but did not improve VO_{2 max} or fasting glucose.
Damirchi, et al⁶⁶	Twenty-one middle-aged men with MetS.	Exercise intervention versus control condition.	Six weeks of aerobic exercise: three sessions per week, for 25–40 min of walking or running at 50–60% VO_{2 peak}. Followed by 6 weeks of detraining.	HOMA-IR improved after 6 weeks of training, but had returned to baseline after 6 weeks of detraining.	Regular exercise improved insulin sensitivity, but needs to be maintained as SI is lost if regular exercise ceases.
Solomon et al⁶⁷	One hundred and five participants, with IGT or T2D.	Observational clinical study.	Twelve to 16 weeks of aerobic exercise training.	Glycosylated haemoglobin, fasting glucose and 2-hour OGTT were improved postintervention in 69%, 62% and 68% of subjects, respectively, while SI improved in 90% of the participants.	Training-induced changes in glycaemic control were related to changes in glucose-stimulated insulin secretion, but not SI. Training-induced changes in β cell function may be a key determinant of training-induced improvements in glycaemic control.
Grieco et al⁶⁸	Forty-five healthy recreationally active young adults.	Randomised controlled trial.	Six-week exercise intervention. Four groups: moderate intensity (50% HRR), vigorous intensity (75% HRR), maximal intensity intervals (95/50% HRR) and non-exercising control group.	There were no significant changes in insulin effectiveness (HOMA and QUICKI in any exercise group.	The exercise intervention did not significantly affect insulin effectiveness in a young adult population as assessed by HOMA or QUICKI.
Chen et al⁶⁹	Twenty-three men and women with MetS and 87 men and women without MetS; mean age 48 and 49 years, respectively.	Pre-exercise versus postexercise intervention comparison.	Three months home-based exercise programme of three x 30-min sessions per week at a moderate intensity of either ‘stepper’ or ‘cardio-dance’.	HOMA-IR was maintained in the non-MetS group (1.8 vs 1.9), but deteriorated in the MetS group (3.6 vs 4.3).	The authors reported that 72% of the non-MetS group but only 39% of the MetS group achieved the minimum exercise compliance, and suggested that this may have affected the poor outcome in the MetS group.
Duvivier et al⁷⁴	Eighteen healthy subjects.	Cross-over design to compare daily regimens of activity and exercise.	Four days of each of the following regimens: (1) 14 hours/day sitting, (2) 13 hours/day sitting +1 hour/day vigorous exercise, (3) 8 hours/day sitting +4 hours/day walking +2 hours/day standing.	OGTTs were undertaken the morning after 4 days on each regimen. AUC for insulin was lower following the walking and standing regimen compared with the others.	Reducing sitting time by walking and standing was more effective than 1 hour of vigorous exercise in maintaining SI.
Earnest et al⁷⁵	Men at risk for IR.	Randomised, controlled, exercise intervention trial.	Three months of eucaloric (12 kcal/kg/week) steady state AER compared with INT.	Twenty-four-hour and 72-hour postexercise fasting OGTT improved. HOMA-IR was improved with INT and AER. Stratification of participants based on pretraining values for HOMA-IR revealed that both low and high HOMA-IR participants demonstrated significant reductions with INT, whereas only high HOMA-IR showed significant improvements with AER.	Eucaloric AER and INT appear to affect fasting glucose OGTT similarly. Both INT and AER benefited those with high HOMA-IR, while INT also benefited those with low HOMA-IR, thereby suggesting that INT may have a greater impact by benefiting across a wider spectrum of HOMA-IR.
Gillen et al⁷⁶	Twenty-five sedentary men (27±8 years).	Randomised control trial.	For 12 weeks, three sessions per week of either: (1) SIT (3 × 20 s maximal sprint, interspersed with 2 min cycling recovery at 50 W), (2) 45 min of moderate intensity cycling at ~75% HR_max (~110W) or (3) non-exercise control. SI was assessed via intravenous glucose tolerance tests.	Both exercise regimens produced significant and similar improvements in SI as measured via intravenous glucose tolerance tests performed before and 72 hours postexercise. Likewise VO_{2 peak} improved (~19%) in both exercise groups, as did skeletal muscle mitochondrial content. There were no statistically significant changes in the control group.	SIT produced similar fitness and SI improvements to prolonged moderate intensity exercise, despite requiring a fivefold lower exercise volume and time commitment.
Shepherd et al⁷⁷	Ninety previously inactive volunteers.	Randomised control trial.	Ten weeks, three sessions per week of either: (1) HIIT (15–60 s with target HR >90% HR_max, with 45–120 s active recovery for a total of 18–25 min, including warm-up) or (2) 30–45 min continuous exercise at an intensity ~70% HR_max.	HOMA improved in both groups, but was achieved with less time commitment and greater adherence in the HIIT group.	HIIT may provide a time-efficient alternative to continuous moderate intensity exercise.
Arad et al⁸⁰	Twenty-eight overweight/obese African American women.	Randomised control trial, with diet determined to maintain body weight. Exercise intervention n=14; control n=14.	For 14-weeks, three sessions per week of HIIT (4 × 30–60s at 75–90% HRR with 180–210s at 50% HRR between high intensity bouts) or non-exercise control. SI was assessed using 3-hour euglycaemic-hyperinsulinaemic clamp.	While some parameters of exercise metabolism improved, there were no improvements in SI compared with the control group.	HIIT did not improve SI when weight was maintained.
Lanzi et al⁸¹	Nineteen obese men.	Randomised control trial.	Two-week exercise intervention, four sessions per week of either: (1) HIIT (10 × 60 s at 90% HR_max, with 60s recovery) or (2) 40–50 min continuous exercise at an intensity identified as that eliciting Fat_max.	Aerobic fitness improved in both groups, but HOMA2-IR only improved in the Fat_max group.	In the short term (2 weeks) exercise training of a continuous moderate intensity (Fat_max) was more effective than HIIT at improving glycaemic control.
Fisher et al⁸²	Twenty-eight sedentary overweight/obese men (20±1.5 years).	Randomised control trial.	Six weeks, five sessions per week of either: (1) HIIT (20 minutes comprising repeated bouts of 30 s at 85% of peak Wingate power with 4 min recovery at 15% of peak Wingate power) or (2) 45–60 min continuous exercise at an intensity of 55–65% VO_{2 max}.	Postintervention, both exercise groups displayed improvements in SI but neither exercise group displayed statistically significant improvements in HOMA-IR.	Both exercise regimens improved SI, as determined by OGGT, but not HOMA-IR (fasting insulin (µU/ml) x fasting glucose (mmol/L)).
Matsuo et al⁸³	Twenty-six men with metabolic risk factors.	Randomised control trial.	Eight-week exercise intervention, three sessions per week of either: (1) HIIT, (3 × 3 min at~80–85% VO_{2 peak} with 2 min recovery at 50% VO_{2 peak} or (2) 40 min at 60–65% VO_{2 peak}, followed by 4 weeks of a low-calorie diet.	Both exercise interventions showed trends for improving HOMA-IR, and this was statistically significant in the HIIT group after the subsequent 4-week low-calorie diet.	SI trended towards improvement with both HIIT and moderate intensity exercise, and was further improved with the low-calorie diet in the HIIT group.
Inoue et al⁸⁷	Forty-five postpubertal, obese adolescents.	Preintervention versus postintervention comparing an aerobic exercise regimen (AT), with two exercise regimens that included both aerobic exercise and resistance exercise (LP and DUP).	Twenty-six weeks of exercise intervention, 3 × 60 min sessions a week.	SI (HOMA-IR) improved in both the groups undertaking combined aerobic and resistance training, but statistically significant improvements were not found in the group undertaking aerobic exercise without resistance training (AT).	The combination of aerobic plus resistance exercise improved SI more effectively than aerobic exercise alone.
Dâmaso et al⁸⁸	One hundred and sixteen obese adolescents.	Preintervention versus postintervention comparing: (1) aerobic exercise regimen with (2) aerobic exercise plus resistance exercise regimen.	One year of: (1) an aerobic exercise regimen or (2) aerobic exercise plus resistance exercise.	SI measured as HOMA-IR.	While both exercise regimens improved important clinical parameters, the ‘aerobic plus resistance exercise’ regimen produced better metabolic outcomes than aerobic exercise alone.
Nikseresht et al⁸⁹	Thirty-four sedentary, obese, middle-aged men.	Exercise, interventions versus control condition.	Twelve weeks of three sessions per week of: (1) 40–65 min of resistance training; (2) aerobic INT (4 × 4 min at 80–90% HR_max, with 3 min recovery between intervals); (3) non-exercise control.	Fasting HOMA-IR.	Compared with control condition, both aerobic interval training and resistance training were equally effective in reducing IR.
Conceição et al⁹⁰	Twenty postmenopausal women.	Exercise intervention, randomised controlled trial.	Resistance training: 10 exercises, with 3 × 8–10 maximal repetitions three times per week.	Compared with the control group, the resistance training group displayed decreases in fasting blood glucose.	Resistance training performed three times a week may reduce the MetS Z-score with concomitant decreases in fasting blood glucose.
Molsted et al⁹¹	Twenty-three patients treated by dialysis, with (n=14) and without (n=9) IGT.	Control period, followed by the exercise intervention.	Sixteen weeks of strength training three times a week.	After the strength training, fasting insulin, 2-hour insulin and ‘area under the curve’ insulin were significantly lower in patients with IGT or T2D.	Strength training was associated with a significant improvement in glucose tolerance in patients with IGT or T2D undergoing dialysis. The effect was not associated with muscle hypertrophy.
Mavros et al⁹²	One-hundred and three older adults with T2D.	Participants were randomised to the resistance training intervention or non-exercise control group.	Twelve months of resistance training 3 days per week, or sham exercise.	Within the resistance training group, changes in HOMA2-IR were associated with changes in skeletal muscle mass and fat mass. Changes in visceral adipose tissue tended to be related to changes in HOMA2-IR.	Improvements in metabolic health in older adults with T2D were mediated through improvements in body composition, only if they were achieved through high intensity progressive resistance training.
Garnett et al⁹³	One hundred and eleven obese adolescents with prediabetes and IR.	Repeated measures, exercise intervention with groups differing in dietary regimen.	Twelve weeks of 45–60 min, moderate to vigorous circuit training, twice a week.	OGTT following an overnight fast.	SI improved within 12 weeks of commencing the exercise intervention and was still improved compared with baseline at 12 months.
Trussardi Fayh et al⁹⁷	Forty-eight obese individuals, aged 31.8±6.0 years.	Randomised clinical trial.	Participants were allocated to a diet-only group or a diet and exercise group. The intervention was maintained until 5% of the initial body weight was lost.	Both regimens produced significant and similar decreases of visceral adipose tissue and HOMA-IR.	Five per cent weight loss reduced abdominal fat and IR in obese individuals, but exercise did not add to the effect of weight loss on the outcome variables.

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AER, aerobic training; AUC, area under the curve; CRF, cardiorespiratory fitness; DI, Disposition Index; Fat_max, maximal fat utilisation; HIIT, high intensity interval training; HOMA-IR, homoeostasis model of assessment-insulin resistance; HRR, heart rate reserve; INT, interval training; IR, insulin resistance; IFG, impaired fasting glucose; IGT, impaired glucose tolerance; IRS-1, insulin receptor substrate 1; MetS, metabolic syndrome; MIIT, moderate intensity interval training; OGTT, oral glucose tolerance test; PA, physical activity; QUICKI, Quantitative SI Check Index; SI, insulin sensitivity; SIT, sprint interval training; T2D, type 2 diabetes; TER, traditional exercise recommendation.

In presenting this review, the authors acknowledge the growing evidence for the adverse effects of sedentary behaviour on diabetes risk and SI, and that this aspect of behaviour needs to be considered in the wider context of metabolic health. Likewise that exercise may benefit the SI of patients with a number of chronic disease conditions, such as cancers, but due to word limits, these scenarios were beyond the scope of this review.

Molecular mechanisms for exercise-induced changes in SI and glycaemic control

Glucose uptake into skeletal muscle occurs via facilitated diffusion down the diffusion gradient through the presence of the glucose transporter GLUT4 in the sarcolemma and T tubules. A single bout of exercise promotes acute increases in glucose uptake into the skeletal muscle, both during the exercise bout and for some hours postexercise. This increase occurs as a result of GLUT4 being translocated from intracellular sites to the sarcolemma and T tubules, thereby increasing the sites at which glucose can diffuse into the muscle. For a detailed review of the processes resulting in increased glucose uptake during exercise, readers are directed to that by Richter and Hargreaves.¹⁰

In summary, during a bout of exercise the increased contraction-stimulated glucose uptake is linked to increases in AMP-activated protein kinase (AMPK), which results in the phosphorylation of the Rab-GTPase-activating protein TBC1D1.¹¹ This phosphorylation appears to inactivate the TBC1D1, although there is some suggestion that the TBC1D1 needs to be phosphorylated at both the AMPK and Akt sites for deactivation to occur.¹² Since active TBC1D1 has an inhibitory effect, its deactivation enables GTP to react with Rab proteins on the GLUT4 vesicles, and as a consequence there is an increase in GLUT4 vesicle translocation and glucose uptake into the cell.

It appears that a slightly different pathway is used to regulate glucose uptake at rest, and involves TBC1D4 (also known as AS160), the paralogue of TBC1D1. TBC1D4 is involved in the insulin stimulated regulation of GLUT4 translocation and glucose uptake in adipocytes and myocytes. Insulin promotes the phosphorylation of TBC1D4 causing its deactivation and thereby increasing GLUT4 activity. TBC1D4 is also involved in the regulation of glucose uptake postexercise, when increases in SI are associated with elevated intracellular kinase Akt, which results in the phosphorylation of TBC1D4.¹¹ TBC1D4 has similar properties to TBC1D1 and produces similar effects, in that the active form TBC1D4 promotes the hydrolysis of GTP to GDP on Rab proteins, thereby preventing the translocation of GLUT4 to the cell membrane. Whereas when TCB1D4 is phosphorylated and deactivated the GTP reaction with Rab proteins increase GLUT4 translocation to the cell membrane and T tubules, which elevates SI.¹³ However, in contrast with TCB1D1, TCB1D4 appears to display a delayed response to exercise/contraction stimuli, with its deactivation exerting an effect postexercise rather than during exercise,¹¹ an effect which has also been reported in rats.¹⁴ Regular exercise training may also result in chronic improvements in TBC1D4 phosphorylation and thereby increase basal SI.¹¹

Repeated exercise bouts (exercise-training) have been demonstrated to increases GLUT4 concentrations in populations with metabolic syndrome (MetS) and T2D,¹⁵ and these increases are associated with changes in SI.^{6 16 17} Such improvements are tissue specific, as exercise appears to improve skeletal muscle but not hepatic SI, nor insulin-stimulated glucose uptake in adipose tissue.^{18 19} In addition to this, the improvements are primarily located in the muscle fibres undertaking most of the work during the exercise.²⁰

Other molecules associated with the SI regulatory processes include insulin receptor substrate 1 (IRS-1) and IRS-2. While the precise roles of these receptor molecules require further elucidation, it is evident that they are activated by the insulin receptor tyrosine kinase and promote the phosphorylation/activation of Akt,²¹ thereby promoting glucose uptake into the cell. Reduced p-IRS-1 (ser⁶¹²) phosphorylation has been reported in obese and obese insulin-resistant subjects, suggesting an association between lower concentrations of activated IRS-1 and impaired SI, whereas acute increases in IRS-1 phosphorylation have been demonstrated following a single 60 min bout of moderate intensity exercise (60% VO_{2 peak}), suggesting an association with increased activation of IRS-1 and improved SI.²²

Obesity, excess lipid availability and SI

It is well established that obesity and an associated excess of available lipids results in a loss of SI in skeletal muscle, and this may be linked with impaired deactivation of TCB1D4.²³ Paradoxically, while obesity increases intramyocellular triglyceride (IMTG) concentrations, so does endurance exercise training.²⁴ Yet the skeletal muscles of obese sedentary individuals have a compromised SI, while those of well trained endurance athletes are highly insulin sensitive.^{24 25} Hence there must be a distinct molecular basis, other than differences in IMTG concentration to explain their contrasting SI characteristics. Diacylglycerols (DAGs) and ceramides are lipid intermediates that have been proposed to explain this apparent paradox. However, while some studies have demonstrated that exercise can reduce DAGs in previously inactive obese individuals, with a concomitant increase in SI,^{25 26} the causative role of DAGs has been questioned as the muscles of endurance trained athletes have been shown to have nearly twice the DAG content of obese sedentary individuals and have a 50% higher DAG content than normal-weight sedentary individuals.^{27 28}

Conversely, evidence is accumulating for the view that ceramides (sphingolipid metabolites) may be the causal link between saturated fatty acid content (but not unsaturated fats) in skeletal muscle and impaired SI.^27–29 In the acute phase, exercise has been demonstrated to increase serum ceramide,³⁰ but these returned to basal levels 2 hours postexercise, while the sphingolipids lipids measured in this study were not elevated during exercise but declined to below basal levels postexercise. However, exercise training has been demonstrated to reduce plasma ceramides and these changes are negatively correlated with increased SI.³¹ An explanation for the molecular link between ceramides and SI is through the presence of excess saturated FFA.²⁹ This explanation suggests that the excess saturated FFA and associated high ceramide content inhibits Akt/PKB phosphorylation and activation by protein phosphatase 2A, thereby preventing the translocation of Akt/PKB from the cytoplasm to the membrane. This may then link to the aforementioned effects on the activation of other signalling molecules, leading to an impaired translocation of GLUT4 to the membrane.

Updates to acute SI responses to exercise

Studies assessing the acute responses during or immediately following a single bout of aerobic exercise suggest that SI is improved by more than 50% for up to 72 hours after the last exercise bout.⁶ However, this acute improvement in SI is lost within 5 days after the last exercise bout, even in highly trained subjects.⁶

Table 1 summarises recent studies that assessed acute responses to exercise on SI. Rynders et al’s study confirms the previously reported improvements in SI in patients with prediabetes of around 50% 1 hour after aerobic exercise.³⁸ Likewise, Newsom et al reported an increase in SI in sedentary obese adults the day after moderate intensity exercise,³⁹ indicating that the acute response was evident for some hours. However, whereas Rynders et al³⁸ reported higher intensity exercise to produce greater improvements in SI (85% following high intensity exercise vs 51% following moderate intensity exercise), Newsom et al³⁹ found that it did not. Indeed Newson et al reported that their lower intensity (50% VO_{2 peak}) but longer duration bout of the same calorific cost was more effective as it resulted in a statistically significant 35% improvement in SI, whereas their bout at 65% VO_{2 peak} only resulted in a 20% increase that was not statistically significant. The discrepancy between these studies may at least in part be due to the differences in the ‘higher’ exercise intensities used in these studies, with Newsom et al’s being of a more ‘moderate’ rather than ‘high’ intensity.

In a more recent study, Malin et al⁴⁰ sought to identify the acute impact of exercise intensity on different components of insulin sensitivity and indicators of glycaemic control, including: glucose-stimulated insulin secretion (GSIS), skeletal muscle IR (SM_IR), hepatic IR (HOMA_IR) and adipose IR (ADIPOSE_IR). In their study they administered a 75 g OGTT 1 hour postexercise/control and in their analyses they assessed the relationship between the aforementioned measures and reported that exercise lowered SM_IR independently of exercise intensity, but that compared with controls, high intensity exercise (200 kcal cycle ergometer exercise bout at 75% of difference between lactate threshold and VO_{2 peak}) increased HOMA_IR and ADIPOSE_IR: which may initially appear contradictory. However, since GSIS was not reduced postexercise and the DIs of the hepatic and adipose tissues were lowered with high intensity exercise, while that of muscle increased, it resulted in a lower postprandial blood glucose. Based on these findings the authors suggest that insulin secretion from the pancreas matches the combined requirements of these tissues and there is some communication between them to produce this outcome. They also suggest that the elevated HOMA_IR and ADIPOSE_IR may be beneficial postexercise, as it could promote greater glucose uptake into the skeletal muscle, in which IR is lower, and thereby more effectively promote the restoration of muscle glycogen postexercise.

The variable of exercise intensity is manipulated and taken to greater extremes through the prescription of ‘high-intensity interval training’ or sprint interval training (SIT), in which relatively short bursts of high intensity exercise are interspersed with lower intensity activity or rest recovery.⁴¹ Gibala et al⁴² propose that the term HIIT be used when repeated short bouts of exercise at intensities of between 80%HR_max and 100% HR_max are used, while protocols that involve repeated short bouts of maximal ‘all-out’ exercise at intensities greater than the work rate that elicits VO_{2 max} be classified as SIT.

In the context of studies assessing the impact of short duration, high intensity exercise, including HIIT and SIT, Ortega et al⁴³ found that while their high intensity intervals (four 30 s sprints), continuous low intensity (46% VO_{2 peak}) and moderate intensity (77% VO_{2 peak}) exercise bouts all improved insulin sensitivity in healthy men for at least 48 hours, the repeated sprints produced the greatest short-term effects 30 min postexercise. Similarly, the study by Terada et al⁴⁴ reported that 60 min of HIIT (repetitions of 3 min at 40% VO_{2 peak} and 1 min at 100% VO_{2 peak}), reduced overnight and fasting glycaemia the day after the exercise by more than a bout of continuous moderate intensity exercise at 55% of VO_{2 peak}. They also reported that exercising in a fasted state rather than ‘post-breakfast’ attenuated postprandial glycaemic increments; and compared with the control condition, HIIT in a fasted state produced significant improvements to: 24 hour mean glucose, fasting glucose, postprandial glycaemic increment, glycaemic variability and time spent in hyperglycaemia.

In comparison, Whyte et al compared four maximal 30 s sprints with 4.5 min recovery between each (SIT) and a single maximal extended sprint matched for work done.⁴⁵ The day following exercise, the SIT session had failed to improve SI over a control (no exercise) condition, but the extended sprint had improved SI by 45%. Hence the failure of SIT to improve SI in this study contradicts the findings of Ortega et al⁴³ but raises the possibility of a single bout of high intensity exercise of relatively short duration (approximately 2–3 min) being sufficient stimulus to promote the regulatory processes underlying improvements in SI, and this requires further elucidation.

Updates on the association between SI and PA—lifestyle studies

Table 2 summarises the results from recent studies assessing potential links between a lifestyle involving regular PA and SI. Uemura et al’s⁴⁶ survey confirms previous work that demonstrates a link between a lifestyle involving PA and better glycaemic control, as did Rosenberger et al⁴⁷ who reported that a lifestyle involving regular walking and other activities reduced by 50% the OR for MetS. Similarly, Caro et al⁴⁸ reported a significantly lower (21 vs 46%) prevalence of MetS in people who complied with the aerobic exercise guidelines of 30–60 min of moderate activity 5 days per week.

Table 2.

Summary of recent studies assessing the association between regular PA and SI

Reference	Participants	Study type	PA or other data collected	Outcome measure	Authors' conclusions and comments
Uemura et al⁴⁶	Five hundred and eighteen eligible subjects (380 men and 138 women) who attended the Tokushima Prefectural General Health Checkup Center.	Survey.	Questionnaire on lifestyle characteristics, including leisure-time exercise and daily non-exercise activities.	Subjects with longer durations of daily non-sedentary activities had significantly lower adjusted ORs for MetS. Daily non-sedentary activities were associated with lower HOMA-IR.	A lifestyle involving greater time spent in non-sedentary activities reduced the risk of IR.
Rosenberger et al⁴⁷	Three hundred and one overweight/obese patients with prediabetes.	Survey of PA habits.	Participants reported walking and other activities, and were assessed for factors associated with MetS. Participants were categorised as those with and those without MetS.	Eighteen per cent of subjects with MetS reported at least 150 min of activity minutes per week compared with 29.8% of those without MetS. The odds of MetS was lower with greater activity minutes.	Meeting PA goals of 150 min/week, reduced MetS odds in overweight/obese adults with prediabetes.
Caro et al⁴⁸	One hundred and one adults with no personal history of disease aged 30–70 years.	A cross-sectional, observational study in an adult population. Participants were age-matched and sex-matched for comparison.	Participants were classified into: (1) those who undertook regular exercise of 30–60 min of moderate physical exercise 5 days per week, and (2) non-exercising controls who exhibited a sedentary lifestyle.	Indicators of fasting plasma insulin levels HOMA-IR were significantly lower in the regular PA group. Prevalence rates of MetS were 20.7% and 45.8% in the regular PA and sedentary groups, respectively.	Moderate regular PA is associated with higher SI.
Jiménez-Pavón et al⁴⁹	One thousand and fifty-three boys and girls, aged 12.5–17.5 years.	A cross-sectional study in a school setting.	PA was assessed via accelerometry; CRF assessed via a 20 m shuttle run test. Fasting insulin and glucose concentrations were measured. The HOMA-IR and quantitative SI index were calculated.	In men, VPA was negatively associated with markers of resistance (IR) after adjusting for confounders including waist circumference. In women moderate PA, moderate PA to VPA, and average PA were negatively associated with markers of IR after adjusting for confounders. When the sample was segmented by CRF levels, all the PA intensities were significantly negatively associated with the markers of IR in women with low CRF but not in those with middle-high CRF after adjusting for confounders.	The findings suggest that PA is negatively associated with markers of IR after adjusting for confounders including total and central body fat in both sexes. This relationship is modified by the CRF levels, which are especially important in those women with low CRF. Preventive strategies should focus on increasing the volume of PA and on enhancing CRF through VPA.
Telford et al⁵⁰	Seven hundred and eight primary school children, mean age 8.1±0.35 years.	Four-year cluster-randomised intervention study into the effects of specialists versus non-specialists delivering physical education classes.	The intervention involved the employment of specialist physical education teachers to deliver PE classes (intervention) in primary schools, rather than delivery by generalist primary teachers (control).	The PE classes delivered by the PE specialists involved more fitness work than the control PE classes delivered by primary generalists (7 min vs 1 min) and more moderate PA (17 min vs 10 min, respectively). There were no differences at baseline, but by grade 6, the intervention had lowered the prevalence of IR by 14% in the boys and by 9% in the girls, also the percentage of children with IR > 3 (a cut-off point for metabolic risk) was lower in the intervention than in the control group (combined, 22% vs 31%; boys, 12% vs 21%; girls, 32% vs 40%).	Specialist-taught primary school PE increased physical activity in PE classes, and was associated with a lower prevalence of IR in community-based children.

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CRF, cardiorespiratory fitness; HOMA-IR, homoeostasis model of assessment-insulin resistance; IR, insulin resistance; MetS, metabolic syndrome; PA, physical activity; SI, insulin sensitivity; VPA, vigorous physical activity.

The importance of lifestyle is evident even in young people as a survey of children found that PA was negatively associated with markers of IR,⁴⁹ and the study by Telford et al found that the prevalence of IR was reduced in primary school age children when PA was increased in school.⁵⁰

Updates from studies assessing the effects of exercise training upon SI

Exercise training studies generally report health benefits for the majority of participants, providing the exercise dose is of an appropriate intensity, frequency, duration and is undertaken for sufficient time.^{7 20} Meta-analyses and reviews indicate that regular aerobic exercise that complies with exercise prescription guidelines,⁵¹ increases SI by ~25–50%.^{6 8} This training adaptation is likely induced by the increased activity of the muscle fibres, since low intensity aerobic activity, which primarily uses type 1 fibres, induces changes in type 1 fibres expressing myosin heavy chain (MHC) I, but not type 2 fibres (expressing MHC IIA or MHC IIX).¹² If such adaptations are specific to the fibres that experience increased activity, then this presents the possibility of higher intensity exercise, which involves a greater recruitment of the type 2 fibres, inducing beneficial adaptations in both type 1 and type 2 fibres.

Aerobic exercise interventions, including the assessment of the influence of exercise volume and intensity

Table 3 summarises the results of recent studies assessing the effect of exercise interventions on SI. Studies consistently show that moderate aerobic exercise for 30 min or more, three or more times a week for 8 weeks or more improves SI and other markers of glycaemic control. This has been reported in a range of populations including women with diabetes,⁵²men and women with diabetes and IGT^{53 54} obese men,⁵⁵ obese women,⁵⁶ obese and overweight postmenopausal women,³⁸ obese adolescents,^{57 58} obese patients,⁵⁹ sedentary moderately overweight young men,^{19 60} subjects with MetS,⁶¹ older obese adults with IGT,³⁴ obese adolescent girls,⁶² and adults with T2DM and non-alcoholic fatty liver disease.⁶³

A commonly advocated exercise prescription of three sessions per week of 30 min walking was used by Motahari-Tabari et al⁶⁴ who reported improvements in the homoeostatic model assessment of insulin resistance (HOMA-IR), fasting plasma insulin and fasting glucose in women with T2D, following 8 weeks of this aerobic exercise regimen. Likewise, Herzig et al⁶⁵ found that 60 min of walking, three times a week for 3 months in patients with prediabetes improved HOMA-IR, fasting and 2-hour insulin, despite no improvements in fasting 2-hour glucose or VO_{2 max}. The finding of an improvement in SI without an improvement in VO_{2 max} concurs with some previously mentioned studies.³⁷

Damirchi et al⁶⁶ also demonstrated an improvement in insulin sensitivity in middle-aged men with MetS following a 6-week aerobic exercise programme of three sessions per week of 25–40 min walking or running at 50–60% VO_{2 peak}, and also reported the interesting finding that this benefit was lost within 6 weeks of detraining. Solomon et al reported improvements in glucose-stimulated insulin secretion, but not SI in participants with T2D or IGT,⁶⁷ and suggested that training-induced changes in β cell function may be a key determinant of training-induced improvements in glycaemic control. Additionally, Skleryk et al did not find any beneficial changes from 5 days a week of aerobic exercise at 65% VO_{2 peak} in overweight/obese sedentary men,⁵⁵ but their exercise intervention was only for 2 weeks and may not have been of sufficient duration to induce detectable changes. Likewise, Grieco et al’s study on recreationally active young adults did not change insulin effectiveness,⁶⁸ although, given that the participants were already recreationally active it may be that their prestudy values were not sufficiently poor to be changed by the relatively short 6-week intervention.

Chen et al’s study also produced results that were not in accordance with similar studies and they suggested that this may have been due to participants’ lack of compliance and exercise intensity,⁶⁹ with their non-MetS group attaining greater compliance and thereby maintaining their SI, while their MetS group displayed poorer compliance, which may have contributed to their decline in SI.

One exercise variable that is subject to manipulation in exercise interventions is that of exercise volume, and in general, studies that have examined a possible dose response report additional benefits from higher exercise doses (>1900 kcal/week), with increases in SI and improved β cell function in adults with prediabetes.³⁴ While such levels of activity may be desirable, compliance is often low even for much lower exercise volumes^70–73 and for those who are unable to meet these levels it is evident that much of the health benefit is attained from an exercise dose of only~1900 kcal/week or even less, with only minor additional benefits to fitness, body fat and insulin sensitivity when exercising for 600 kcal/day compared with 300 kcal/day.⁶⁰ Indeed the commonly prescribed dose of 5 × 30 min of moderate intensity exercise/week, which is reported on numerous occasions to be effective, would be around 475–950 kcal/week.

Another exercise variable that is receiving attention in the research is that of exercise intensity, as many of the adaptations that play a role in the exercise-induced increases in SI display a response that is related to the intensity of the activity. For example, while low-intensity training such as walking for 30 min, 3–4 days per week, for 6 months improves markers of glycaemic control (such as ‘area under the curve’ (AUC) for insulin), a further 6 months of higher intensity exercise (jogging 3–4 days per week for 6 months) elicits substantially greater improvements.⁶ Some reviews suggest that higher intensity exercise (>75% of VO_{2 peak}) is more efficacious than lower intensity (<60% of VO_{2 peak}).⁶ However, these findings are equivocal as others have reported that lower intensity activity, such as prolonged bouts of standing and walking are more effective than vigorous exercise of the equivalent energy expenditure in improving insulin sensitivity as indicated by OGTTs.⁷⁴ Hence in the context of sustained bouts of continuous exercise the issue of the relative importance of exercise volume in terms of duration or total calorific cost of the exercise, versus the intensity of the exercise remains to be resolved.

Interval training (HIIT and SIT) that uses repeated brief bouts of exercise at intensities that are greater than those used in exercise sessions involving a more prolonged single continuous exercise bout has been demonstrated to induce significant increases in GLUT4 protein (up to 260%) and SI (25%–35%)17 54 58 75 with the overall outcomes indicating comparable and, in some cases, superior improvements in SI compared with moderate intensity continuous exercise training,⁷⁶ despite it involving substantially less time commitment and reduced total exercise volume. Additionally, Earnest et al⁷⁵ found INT to benefit patients with low HOMA_IR as well as patients with high HOMA_IR, whereas moderate intensity aerobic exercise only benefited the patients with high HOMA_IR. Hence interval training could be beneficial to both, and for those with relatively mild IR it may be more effective in preventing further decline and/or restoring SI. As an extension of this, it may be speculated that HIIT could be a more effective preventative exercise regimen for asymptomatic healthy individuals. Furthermore, Madsen et al³⁶ reported that HIIT improved both HOMA-IR and β cell function in patients with T2D, hence it could be beneficial across the IR spectrum.

Shepherd et al⁷⁷ investigated the efficacy of HIIT in a ‘gym-setting’ with 90 previously inactive volunteers. In this study they reported that both HIIT and moderate intensity exercise improved SI, but HIIT achieved this with less than half the time commitment and greater adherence. Such findings are important given that a ‘lack of time’ remains the most commonly cited barrier to regular exercise participation.^{78 79} This, combined with reports of greater enjoyment when compared with sessions undertaken at a constant high intensity, is likely to improve compliance, although the higher intensity of the exercise may make it unsuitable for some ‘at risk’ individuals with cardiovascular issues. However, not all studies have reported HIIT to improve insulin sensitivity, including those of Arad et al⁸⁰ in which overweight/obese African American women undertook 14 weeks of HIIT (three sessions a week), while maintaining a stable weight and that of Lanzi et al⁸¹ in which moderate intensity exercise was more effective that HIIT in improving HOMA2-IR in obese men, although the exercise intervention for this later study was only 2 weeks. The complexity of the issue is exemplified in the findings of studies such as that of Fisher et al⁸² in which both HIIT and moderate intensity exercise improved SI, as determined by an OGGT, but did not improve IR as determined by HOMA-IR (fasting insulin (µU/ml) x fasting glucose (mmol/L)). In another work, while Matsuo et al⁸³ reported beneficial trends in HOMA-IR following HIIT as well as moderate intensity exercise, the results only reached statistical significance when the participants went on to follow a 4-week low-calorie diet.

Effects of resistance training upon SI

While much of the early research into exercise and SI has focused on aerobic exercise, recent exercise interventions using resistance training (REX) have demonstrated that this mode of exercise can also improve indicators of glycaemic control in a variety of populations, including older overweight individuals with prediabetes⁸⁴ and postmenopausal women.⁸⁵ However, the training adaptations may not always change all indicators of glycaemic control as Eikenberg et al⁸⁴ found that twice weekly resistance training for 12 weeks improved 2-hour OGTT results in their participants who commenced the study with IGT and impaired fasting glucose (IFG), but not in those who commenced with IFG without IGT. Likewise, REX did not alter fasting glucose concentrations, AUC or ISI.

At a molecular level, REX consisting of two to three sessions per week for 8–26 weeks, can increase GLUT 4 concentrations and translocation by 30–70%, and enhance SI by 10–48%.^{6 17 86} Some studies suggest that these improvements could be partially dependent on the training stimulus increasing muscle mass, as well as qualitative changes within the muscle,⁸⁶ with the metabolic adaptations potentially involving changes in the type 2A fibres that are likely to be recruited during REX, as well as type 1 fibres. As indicated elsewhere these adaptations may not be homogenic between fibre types or exercise modalities. Furthermore, since both endurance exercise and REX increase SI, it is possible that a combination of these two training modalities could have additive benefits,¹⁶ particularly if the molecular targets of these activities differ. Indeed the study by Inoue et al found that the combination of aerobic plus resistance exercise was better than aerobic exercise alone at improving insulin sensitivity in postpubertal obese adolescents.⁸⁷ These results concur with the findings of Dâmaso et al⁸⁸ whose findings also suggested that the combination of aerobic and resistance training had better metabolic outcomes than aerobic training (AER) alone for obese adolescents. Furthermore, Nikseresht et al⁸⁹ compared the efficacy of aerobic INT and resistance training and found them to be equally effective in reducing IR and fasting insulin levels, but suggested that the aerobic programme had better anti-inflammatory effects. Consequently the findings of various studies have contributed towards ‘evidence-based’ exercise recommendations now including both aerobic and REX guidelines for healthy individuals.¹⁷

A recent study by Conceição et al⁹⁰ adds further support to the incorporation of REX to improve glycaemic control in postmenopausal women.⁹⁰ However, the influence of changes in muscle mass through resistance exercise requires further elucidation since Molstead et al reported improvements in fasting insulin, 2-hour insulin and the AUC for insulin in patients with IGT or T2D who had no increase in muscle mass,⁹¹ while Mavros et al reported that in their study the improvements in SI (HOMA2-IR) in older patients with T2DM were associated with changes in skeletal muscle mass.⁹² Hence further work is required to elucidate the impact of quantitative (mass) and qualitative changes to the skeletal musculature on SI. Bacchi et al in their study on patients with T2DM and non-alcoholic fatty liver disease, found that both REX and aerobic exercise improved SI and reduced hepatic fat content.⁶³ Likewise, as previously mentioned, Nikseresht et al⁸⁹ found resistance training to be as effective as aerobic INT in reducing IR in obese middle-aged men. Whereas Lee et al did not find REX to improve SI in obese adolescent girls, while aerobic exercise did.⁶²

Circuit training is another variation of exercise mode, including elements of resistance training and HIIT, as the exercise sessions typically involve brief bouts of high intensity muscular resistance exercise interspersed with rest periods. Iin studies involving obese 10–17-year-olds with prediabetes , it has been demonstrated to improve SI when undertaken with a dietary intervention.⁹³

The effect of exercising in a fed or fasted state and other exercise-food manipulations

In 2010, Van Proeyen et al⁹⁴ published a study in which they fed their participants a fat rich (50% of kcal) hypercaloric (~+30% kcal/day) diet for 6 weeks. During this time the participants exercised (cycling and running) four times a week (2 × 60 min and 2 × 90 min). Some of these participants exercised in a fasted state, while others ate a carbohydrate rich breakfast ~90 min before the exercise, as well as receiving a carbohydrate drink during the exercise session (CHO-Fed). There was also a non-exercise control group. The overall outcome of this was that the group who trained in a fasted state did not increase their body mass, unlike those in the control and CHO-fed groups. The fasted group also displayed superior improvements in SI compared with the control group, whereas the CHO-fed group did not. Furthermore the fasted group showed greater increases in GLUT4, and elevated AMPK α phosphorylation. The conclusions being that exercising in a fasted state may enhance the exercise induced benefits to SI, compared with exercising when carbohydrate had been recently ingested. The enhancements of these training effects appear to concur with the improved acute responses when exercising in a fasted state.⁴⁴ In related work, as mentioned previously, Matsuo et al⁸³ reported that beneficial changes in HOMA-IR were enhanced when the participants underwent a low- calorie diet for 4 weeks following the exercise intervention, even though the exercise intervention had ceased, thus further highlighting the interaction between exercise and diet in influencing SI.

Other exercise-feeding manipulations that have received recent attention include undertaking exercise before meals—‘exercise snacks’.⁹⁵ From which, findings indicate that brief bouts of exercise (6 × 1 min incline walking at 90% HR_max) 30 min before main meals improved glycaemic control in individuals with IR.

Exercise, SI and changes to body mass

Numerous studies have reported that exercise induced improvements in SI are independent of changes to body composition or diet induced weight loss, and that the benefits of exercise and weight loss are additive,^{5 6} as reported by de Sousa et al⁹⁶ who found football training coupled with weight loss improved insulin sensitivity and blood lipid profile, whereas weight loss alone did not. However these findings are not unequivocal as some studies report weight loss to be the key component to improving SI, for example, Stuart et al found that AER without weight loss did not improve SI in individuals with MetS, whereas exercise with weight loss did, thereby implying that the main influence on improving SI was weight loss rather than exercise.¹⁵ Similarly, Trussardi Fayhn et al found that exercise training did not add to the effect that weight loss had on improving SI in obese individuals.⁹⁷

By way of comparison, several recent studies suggest that the combination of exercise training and diet is more effective than diet alone in improving SI, and even when the additional benefits of exercise plus diet versus diet alone were modest,^{62 94} the inclusion of exercise improved other markers of metabolic health.⁵³ Likewise, Mavros et al reported that improvements in metabolic health in older patients with T2DM were mediated through improvements in body composition only if they were achieved through high intensity progressive REX.⁹³

Non-responders and adverse responders to exercise interventions

While there is unequivocal evidence for PA improving population and participant group mean values, indicating positive changes in the majority of participants within the data, it is evident that there is considerable variation in the magnitude of response to exercise interventions within the population: with some individuals displaying considerably greater changes in a variety of health-related outcome measures than others, despite adhering to the same exercise regimen.⁹⁸ Additionally, the magnitude of change in one factor, such as VO_{2 peak}, is not necessarily associated with the magnitude of change in another factor. For example, in the HART-D study, a 9-month exercise training intervention for patients with T2DM,⁹⁹ 57% of participants displayed an increase in their peak oxygen uptake (VO_{2 peak}), while the remaining 43% exhibited no change. Of those who did show an improvement, only around two-thirds increased their VO_{2 peak} by >5% (high responders to exercise), and a third displayed <5% increase (low responders to exercise). Yet despite this disparity in the magnitude of change in aerobic capacity, the exercise intervention induced similar improvements in HbA₁_c and body composition (reduction % body fat) in both responders and non-responders for VO_{2 peak}. Hence the improvements in glycaemic control were associated with participating in the exercise training, but were not associated with changes to aerobic fitness, expressed as percentage improvement in VO_{2 peak}, which was also a finding of the study by Herzig.⁶⁵

Furthermore, there is also evidence that a minority of the population may respond adversely to exercise intervention, as reported in the HERITAGE study on 1687 men and women, in which 126 (8.4%) displayed an adverse change (increase >3.5 mU/L) in fasting insulin.¹⁰⁰ The underlying reasons for these adverse changes are unknown, but their elucidation may further the cause of individualised exercise prescription.¹⁰⁰

Conclusion

Recently (published 2013-2016) studies involving PA confirm previous research findings of its efficacy in improving SI. A lifestyle incorporating aerobic exercise and/or PA that complies with the guidelines of being of moderate intensity for at least 30 min on 3–5 days per week, is associated with improved SI and glycaemic control. Acute improvements in SI (2–72 hours postexercise) occur after a single bout of exercise and chronic adaptations are evident from training studies involving interventions undertaken for at least 8 weeks. The benefits of PA/exercise are evident across all ages from children to older adults, including those categorised as asymptomatic/healthy, prediabetic/MetS, and patients with T2DM. However, the findings are not unequivocal and even within studies not all indicators of insulin sensitivity and glycaemic control display improvements. Indeed, even with increases in the expression of IRS-1 and GLUT4, decreases in IR are not guaranteed.⁶⁹ A dose response is sometimes evident, and exercise sessions using higher intensities, including HIIT and SIT can produce greater benefits to SI, but not always. Indeed there remains the question of whether larger volumes of moderate intensity exercise or lower volumes of higher intensity may produce a different magnitude of adaptation, and could do so via stimulating different adaptations. Researchers are also assessing whether lower volume sessions may have the practical advantages of greater compliance, through increased enjoyment and a lesser time commitment, since lack of time is a commonly given reason for non-compliance with exercise recommendations.

While aerobic exercise interventions usually benefit SI, improvements in SI are not always associated with changes to aerobic fitness (VO_{2 max}), for reasons that may be explained by the different adaptations induced by the exercise in the cardiovascular system and peripheral musculature.

REX can improve SI through qualitative changes within the muscle as well as increases in muscle mass but the benefits are not evident in all REX studies. However there is a growing body of evidence for including both aerobic exercise and REX in exercise regimens, as this appears to more effectively improve SI than either mode of exercise alone.

The debate continues over the relative importance of exercise versus weight loss for improving SI and whether the combination of the two is more efficacious for achieving good glycaemic regulation.

The molecular bases for exercise-training induced improvements in SI are linked to increases in GLUT4 concentration and acute exercise-induced increases in Akt that deactivate TCB1D4 increasing GLUT4 translocation to the membrane, an effect that persists for several hours postexercise. Additionally, the increased capillarisation of the skeletal muscle is another factor linked to improved SI. The concentration of ceramides within muscle may provide the casual link between a high concentration of intramuscular saturated fatty acids and impaired SI.

Studies in which improvements to SI were not reported may have been a consequence of their interventions involving exercise intensities that were too low, durations that were too short or a population group whose glycaemic control was relatively good at baseline and/or were already ‘recreationally active’, and hence the capacity to change was limited.

Footnotes

Acknowledgements: The authors thank the reviewers for their helpful and constructive comments that assisted with the finalising of the manuscript.

Contributor: SRB undertook the initial search of the published literature and writing of the paper. JAH provided expert specialist input into the molecular sections, the identification of recent reviews that were used to present the established knowledge and contributed to the writing and revisions to the final manuscript.

Competing interests: None declared.

Provenance and peer review: Not commissioned; externally peer reviewed.

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PERMALINK

Update on the effects of physical activity on insulin sensitivity in humans

Stephen R Bird

John A Hawley

Abstract

Purpose and methods

Discussion and conclusions

Key messages.

Introduction

Methods

Table 1.

Table 3.

Molecular mechanisms for exercise-induced changes in SI and glycaemic control

Obesity, excess lipid availability and SI

Other molecular and physiological changes linked to exercise induced improvements to SI

Updates to acute SI responses to exercise

Updates on the association between SI and PA—lifestyle studies

Table 2.

Updates from studies assessing the effects of exercise training upon SI

Aerobic exercise interventions, including the assessment of the influence of exercise volume and intensity

Effects of resistance training upon SI

The effect of exercising in a fed or fasted state and other exercise-food manipulations

Exercise, SI and changes to body mass

Non-responders and adverse responders to exercise interventions

Conclusion

Footnotes

References

ACTIONS

PERMALINK

RESOURCES

Cite

Add to Collections

PERMALINK

Update on the effects of physical activity on insulin sensitivity in humans

Stephen R Bird

John A Hawley

Abstract

Purpose and methods

Discussion and conclusions

Key messages.

Introduction

Methods

Table 1.

Table 3.

Molecular mechanisms for exercise-induced changes in SI and glycaemic control

Obesity, excess lipid availability and SI

Other molecular and physiological changes linked to exercise induced improvements to SI

Updates to acute SI responses to exercise

Updates on the association between SI and PA—lifestyle studies

Table 2.

Updates from studies assessing the effects of exercise training upon SI

Aerobic exercise interventions, including the assessment of the influence of exercise volume and intensity

Effects of resistance training upon SI

The effect of exercising in a fed or fasted state and other exercise-food manipulations

Exercise, SI and changes to body mass

Non-responders and adverse responders to exercise interventions

Conclusion

Footnotes

References

ACTIONS

PERMALINK

RESOURCES

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