Skip to main content
PMC home page
The EMBO Journal logoLink to The EMBO Journal
. 1984 Feb;3(2):257–262. doi: 10.1002/j.1460-2075.1984.tb01794.x

Regulated expression of a gene important for replication of plasmid F in E. coli.

L Søgaard-Andersen, L A Rokeach, S Molin
PMCID: PMC557332  PMID: 6325163

Abstract

Fusions between the gene encoding the E protein of the IncFI plasmid F and the lac genes were constructed. Analysis of the expression of beta-galactosidase from these fusions shows that the promoter for the E protein gene is located between the incB region and the structural gene for the E protein. Near this promoter is a regulatory site on which a negative control element acts. Most likely the E protein itself acts as a repressor of E gene expression and thus autoregulates its own expression. No other gene products seem to affect the expression of the E protein gene.

Full text

PDF
262

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. An G., Friesen J. D. Plasmid vehicles for direct cloning of Escherichia coli promoters. J Bacteriol. 1979 Nov;140(2):400–407. doi: 10.1128/jb.140.2.400-407.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BERTANI G. Studies on lysogenesis. I. The mode of phage liberation by lysogenic Escherichia coli. J Bacteriol. 1951 Sep;62(3):293–300. doi: 10.1128/jb.62.3.293-300.1951. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bergquist P. L., Downard R. A., Caughey P. A., Gardner R. C., Lane H. E. Analysis of mini-F plasmid replication by transposition mutagenesis. J Bacteriol. 1981 Sep;147(3):888–899. doi: 10.1128/jb.147.3.888-899.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bex F., Karoui H., Rokeach L., Drèze P., Garcia L., Couturier M. Mini-F encoded proteins: identification of a new 10.5 kilodalton species. EMBO J. 1983;2(11):1853–1861. doi: 10.1002/j.1460-2075.1983.tb01671.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bolivar F. Construction and characterization of new cloning vehicles. III. Derivatives of plasmid pBR322 carrying unique Eco RI sites for selection of Eco RI generated recombinant DNA molecules. Gene. 1978 Oct;4(2):121–136. doi: 10.1016/0378-1119(78)90025-2. [DOI] [PubMed] [Google Scholar]
  6. Bolivar F., Rodriguez R. L., Greene P. J., Betlach M. C., Heyneker H. L., Boyer H. W., Crosa J. H., Falkow S. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. Gene. 1977;2(2):95–113. [PubMed] [Google Scholar]
  7. Casadaban M. J., Chou J., Cohen S. N. In vitro gene fusions that join an enzymatically active beta-galactosidase segment to amino-terminal fragments of exogenous proteins: Escherichia coli plasmid vectors for the detection and cloning of translational initiation signals. J Bacteriol. 1980 Aug;143(2):971–980. doi: 10.1128/jb.143.2.971-980.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Casadaban M. J., Cohen S. N. Analysis of gene control signals by DNA fusion and cloning in Escherichia coli. J Mol Biol. 1980 Apr;138(2):179–207. doi: 10.1016/0022-2836(80)90283-1. [DOI] [PubMed] [Google Scholar]
  9. Cohen S. N., Chang A. C., Hsu L. Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA. Proc Natl Acad Sci U S A. 1972 Aug;69(8):2110–2114. doi: 10.1073/pnas.69.8.2110. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Collins J., Pritchard R. H. Relationship between chromosome replication and F'lac episome replication in Escherichia coli. J Mol Biol. 1973 Jun 25;78(1):143–155. doi: 10.1016/0022-2836(73)90434-8. [DOI] [PubMed] [Google Scholar]
  11. Ebbers J., Eichenlaub R. Complementation of replication-deficient deletion derivatives of plasmid mini-F. J Bacteriol. 1981 Sep;147(3):736–743. doi: 10.1128/jb.147.3.736-743.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Eichenlaub R., Figurski D., Helinski D. R. Bidirection replication from a unique origin in a mini-F plasmid. Proc Natl Acad Sci U S A. 1977 Mar;74(3):1138–1141. doi: 10.1073/pnas.74.3.1138. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Gardner R. C., Malcolm L., Bergquist P. L., Lane H. E. IncD, a genetic locus in F responsible for incompatibility with several plasmids of the IncFI group. Mol Gen Genet. 1982;188(2):345–352. doi: 10.1007/BF00332699. [DOI] [PubMed] [Google Scholar]
  14. Kahn M. L., Figurski D., Ito L., Helinski D. R. Essential regions for replication of a stringent and a relaxed plasmid in Escherichia coli. Cold Spring Harb Symp Quant Biol. 1979;43(Pt 1):99–103. doi: 10.1101/sqb.1979.043.01.015. [DOI] [PubMed] [Google Scholar]
  15. Komai N., Nishizawa T., Hayakawa Y., Murotsu T., Matsubara K. Detection and mapping of six miniF-encoded proteins by cloning analysis of dissected miniF segments. Mol Gen Genet. 1982;186(2):193–203. doi: 10.1007/BF00331850. [DOI] [PubMed] [Google Scholar]
  16. Light J., Molin S. Post-transcriptional control of expression of the repA gene of plasmid R1 mediated by a small RNA molecule. EMBO J. 1983;2(1):93–98. doi: 10.1002/j.1460-2075.1983.tb01387.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Light J., Molin S. Replication control functions of plasmid R1 act as inhibitors of expression of a gene required for replication. Mol Gen Genet. 1981;184(1):56–61. doi: 10.1007/BF00271195. [DOI] [PubMed] [Google Scholar]
  18. Lovett M. A., Helinski D. R. Method for the isolation of the replication region of a bacterial replicon: construction of a mini-F'kn plasmid. J Bacteriol. 1976 Aug;127(2):982–987. doi: 10.1128/jb.127.2.982-987.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Manis J. J., Kline B. C. F plasmid incompatibility and copy number genes: their map locations and interactions. Plasmid. 1978 Sep;1(4):492–507. doi: 10.1016/0147-619x(78)90007-0. [DOI] [PubMed] [Google Scholar]
  20. Murotsu T., Matsubara K., Sugisaki H., Takanami M. Nine unique repeating sequences in a region essential for replication and incompatibility of the mini-F plasmid. Gene. 1981 Nov;15(2-3):257–271. doi: 10.1016/0378-1119(81)90135-9. [DOI] [PubMed] [Google Scholar]
  21. Seelke R. W., Kline B. C., Trawick J. D., Ritts G. D. Genetic studies of F plasmid maintenance genes involved in copy number control, incompatability, and partitioning. Plasmid. 1982 Mar;7(2):163–179. doi: 10.1016/0147-619x(82)90075-0. [DOI] [PubMed] [Google Scholar]
  22. Sharp P. A., Hsu M. T., Otsubo E., Davidson N. Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli. I. Structure of F-prime factors. J Mol Biol. 1972 Nov 14;71(2):471–497. doi: 10.1016/0022-2836(72)90363-4. [DOI] [PubMed] [Google Scholar]
  23. Stougaard P., Molin S. Vertical dye-buoyant density gradients for rapid analysis and preparation of plasmid DNA. Anal Biochem. 1981 Nov 15;118(1):191–193. doi: 10.1016/0003-2697(81)90177-9. [DOI] [PubMed] [Google Scholar]
  24. Timmis K., Cabello F., Cohen S. N. Cloning, isolation, and characterization of replication regions of complex plasmid genomes. Proc Natl Acad Sci U S A. 1975 Jun;72(6):2242–2246. doi: 10.1073/pnas.72.6.2242. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Tolun A., Helinski D. R. Direct repeats of the F plasmid incC region express F incompatibility. Cell. 1981 Jun;24(3):687–694. doi: 10.1016/0092-8674(81)90095-7. [DOI] [PubMed] [Google Scholar]
  26. Tolun A., Helinski D. R. Separation of the minimal replication region of the F plasmid into a replication origin segment and a trans-acting segment. Mol Gen Genet. 1982;186(3):372–377. doi: 10.1007/BF00729456. [DOI] [PubMed] [Google Scholar]
  27. Watson L. A., Phua S. H., Bergquist P. L., Lane H. E. An Mr 29000 protein is essential for mini-F maintenance in E. coli. Gene. 1982 Sep;19(2):173–178. doi: 10.1016/0378-1119(82)90003-8. [DOI] [PubMed] [Google Scholar]
  28. Wehlmann H., Eichenlaub R. Plasmid mini-F encoded proteins. Mol Gen Genet. 1980;180(1):205–211. doi: 10.1007/BF00267371. [DOI] [PubMed] [Google Scholar]
  29. Yang R., Lis J., Wu R. Elution of DNA from agarose gels after electrophoresis. Methods Enzymol. 1979;68:176–182. doi: 10.1016/0076-6879(79)68012-6. [DOI] [PubMed] [Google Scholar]

Articles from The EMBO Journal are provided here courtesy of Nature Publishing Group

RESOURCES