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. 1984 Mar;3(3):575–579. doi: 10.1002/j.1460-2075.1984.tb01850.x

The EcoA restriction and modification system of Escherichia coli 15T-: enzyme structure and DNA recognition sequence.

B Suri, J C Shepherd, T A Bickle
PMCID: PMC557390  PMID: 6325176

Abstract

The EcoA restriction enzyme from Escherichia coli 15T- has been isolated. It proves to be an unusual enzyme, clearly related functionally to the classical type I restriction enzymes. The basic enzyme is a two subunit modification methylase. Another protein species can be purified which by itself has no enzymatic activities but which converts the modification methylase to an ATP and S-adenosylmethionine-dependent restriction endonuclease. The DNA recognition sequence of EcoA has an overall structure that is very similar to previously determined type I sequences. It is: 5'-GAGNNNNNNNGTCA-3' 3'-CTCNNNNNNNCAGT-5' where N can be any nucleotide. Modification methylates the adenosyl residue in the specific trinucleotide and the adenosyl residue in the lower strand of the specific tetranucleotide.

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