Skip to main content
PMC home page
The EMBO Journal logoLink to The EMBO Journal
. 1984 Apr;3(4):701–706. doi: 10.1002/j.1460-2075.1984.tb01872.x

Partial characterization of the biosynthesis and integration of the Photosystem II reaction centers in the thylakoid membrane of Chlamydomonas reinhardtii

Philippe Delepelaire 1
PMCID: PMC557414  PMID: 16453511

Abstract

Pulse-labeling of wild-type and a Photosystem II mutant strain of Chlamydomonas reinhardtii was carried out in the presence or absence of inhibitors of either cytoplasmic or chloroplast ribosomes, and their thylakoid membrane polypeptides were analyzed by polyacrylamide gel electrophoresis. A pulse-chase study was also done on the wild-type strain in the presence of anisomycin, an inhibitor of protein synthesis on cytoplasmic ribosomes. The following results were obtained: the Photosystem II reaction center is mainly composed of integral membrane proteins synthesized within the chloroplast. Several of the proteins of the Photosystem II reaction center are post-translationally modified, after they have been inserted in the thylakoid membrane.

Keywords: Chlamydomonas, protein function, protein synthesis, thylakoid membrane, Photosystem II

Full text

PDF
705

Images in this article

702Fig. 1.
on p.702
702Fig. 2.
on p.702
703Fig. 3.
on p.703
704Fig. 4.
on p.704
704Fig. 5.
on p.704

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Burke J. J., Ditto C. L., Arntzen C. J. Involvement of the light-harvesting complex in cation regulation of excitation energy distribution in chloroplasts. Arch Biochem Biophys. 1978 Apr 15;187(1):252–263. doi: 10.1016/0003-9861(78)90031-0. [DOI] [PubMed] [Google Scholar]
  2. Chua N. H., Bennoun P. Thylakoid membrane polypeptides of Chlamydomonas reinhardtii: wild-type and mutant strains deficient in photosystem II reaction center. Proc Natl Acad Sci U S A. 1975 Jun;72(6):2175–2179. doi: 10.1073/pnas.72.6.2175. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Chua N. H., Gillham N. W. The sites of synthesis of the principal thylakoid membrane polypeptides in Chlamydomonas reinhardtii. J Cell Biol. 1977 Aug;74(2):441–452. doi: 10.1083/jcb.74.2.441. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Conde M. F., Boynton J. E., Gillham N. W., Harris E. H., Tingle C. L., Wang W. L. Chloroplast genes in Chlamydomonas affecting organelle ribosomes. Genetic and biochemical analysis of analysis of antibiotic-resistant mutants at several gene loci. Mol Gen Genet. 1975 Oct 3;140(3):183–220. doi: 10.1007/BF00334266. [DOI] [PubMed] [Google Scholar]
  5. Delepelaire P., Chua N. H. Lithium dodecyl sulfate/polyacrylamide gel electrophoresis of thylakoid membranes at 4 degrees C: Characterizations of two additional chlorophyll a-protein complexes. Proc Natl Acad Sci U S A. 1979 Jan;76(1):111–115. doi: 10.1073/pnas.76.1.111. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Diner B. A., Wollman F. A. Isolation of highly active photosystem II particles from a mutant of Chlamydomonas reinhardtii. Eur J Biochem. 1980 Sep;110(2):521–526. doi: 10.1111/j.1432-1033.1980.tb04894.x. [DOI] [PubMed] [Google Scholar]
  7. Grebanier A. E., Coen D. M., Rich A., Bogorad L. Membrane proteins synthesized but not processed by isolated maize chloroplasts. J Cell Biol. 1978 Sep;78(3):734–746. doi: 10.1083/jcb.78.3.734. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Herrmann R. G., Possingham J. V. Plastid DNA-the plastome. Results Probl Cell Differ. 1980;10:45–96. doi: 10.1007/978-3-540-38255-3_3. [DOI] [PubMed] [Google Scholar]
  9. Hurt E., Hauska G. A cytochrome f/b6 complex of five polypeptides with plastoquinol-plastocyanin-oxidoreductase activity from spinach chloroplasts. Eur J Biochem. 1981 Jul;117(3):591–595. doi: 10.1111/j.1432-1033.1981.tb06379.x. [DOI] [PubMed] [Google Scholar]
  10. Lizardi P. M., Luck D. J. The intracellular site of synthesis of mitochndrial ribosomal proteins in Neurospora crassa. J Cell Biol. 1972 Jul;54(1):56–74. doi: 10.1083/jcb.54.1.56. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Mattoo A. K., Pick U., Hoffman-Falk H., Edelman M. The rapidly metabolized 32,000-dalton polypeptide of the chloroplast is the "proteinaceous shield" regulating photosystem II electron transport and mediating diuron herbicide sensitivity. Proc Natl Acad Sci U S A. 1981 Mar;78(3):1572–1576. doi: 10.1073/pnas.78.3.1572. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Miller M. E., Price C. A. Protein synthesis by developing plastids isolated from Euglena gracilis. FEBS Lett. 1982 Oct 18;147(2):156–160. doi: 10.1016/0014-5793(82)81031-4. [DOI] [PubMed] [Google Scholar]
  13. Mullet J. E., Burke J. J., Arntzen C. J. Chlorophyll proteins of photosystem I. Plant Physiol. 1980 May;65(5):814–822. doi: 10.1104/pp.65.5.814. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Nechushtai R., Nelson N. Purification properties and biogenesis of Chlamydomonas reinhardii photosystem I reaction center. J Biol Chem. 1981 Nov 25;256(22):11624–11628. [PubMed] [Google Scholar]
  15. Pfister K., Steinback K. E., Gardner G., Arntzen C. J. Photoaffinity labeling of an herbicide receptor protein in chloroplast membranes. Proc Natl Acad Sci U S A. 1981 Feb;78(2):981–985. doi: 10.1073/pnas.78.2.981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Piccioni R. G., Bennoun P., Chua N. H. A nuclear mutant of Chlamydomonas reinhardtii defective in photosynthetic photophosphorylation. Characterization of the algal coupling factor ATPase. Eur J Biochem. 1981 Jun;117(1):93–102. doi: 10.1111/j.1432-1033.1981.tb06307.x. [DOI] [PubMed] [Google Scholar]
  17. Pick U., Racker E. Purification and reconstitution of the N,N'-dicyclohexylcarbodiimide-sensitive ATPase complex from spinach chloroplasts. J Biol Chem. 1979 Apr 25;254(8):2793–2799. [PubMed] [Google Scholar]
  18. Steinback K. E., McIntosh L., Bogorad L., Arntzen C. J. Identification of the triazine receptor protein as a chloroplast gene product. Proc Natl Acad Sci U S A. 1981 Dec;78(12):7463–7467. doi: 10.1073/pnas.78.12.7463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Wollgiehn R., Parthier B. RNA and protein synthesis in plastid differentiation. Results Probl Cell Differ. 1980;10:97–145. doi: 10.1007/978-3-540-38255-3_4. [DOI] [PubMed] [Google Scholar]

Articles from The EMBO Journal are provided here courtesy of Nature Publishing Group

RESOURCES