Skip to main content
PMC home page
The EMBO Journal logoLink to The EMBO Journal
. 1984 May;3(5):1109–1113. doi: 10.1002/j.1460-2075.1984.tb01937.x

The primary structure of elongation factor EF-1 alpha from the brine shrimp Artemia.

F J van Hemert, R Amons, W J Pluijms, H van Ormondt, W Möller
PMCID: PMC557481  PMID: 6203745

Abstract

cDNA as well as amino acid sequencing has revealed the complete primary structure of elongation factor EF-1 alpha from the brine shrimp Artemia. A comparison with the published sequences of bacterial EF-Tu, mitochondrial EF-Tu and chloroplastic EF-Tu shows that distinct areas of these polypeptide chains are conserved in evolution. The evolutionary distance between prokaryotic and eukaryotic types of EF-Tu is larger than among bacterial and organellar EF- Tus . A number of regions present in both EF-Tu and EF-G from Escherichia coli are also found in EF-1 alpha from Artemia.

Full text

PDF
1110

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Amons R., Pluijms W., Roobol K., Möller W. Sequence homology between EF-1 alpha, the alpha-chain of elongation factor 1 from Artemia salina and elongation factor EF-Tu from Escherichia coli. FEBS Lett. 1983 Mar 7;153(1):37–42. doi: 10.1016/0014-5793(83)80115-x. [DOI] [PubMed] [Google Scholar]
  2. An G., Friesen J. D. The nucleotide sequence of tufB and four nearby tRNA structural genes of Escherichia coli. Gene. 1980 Dec;12(1-2):33–39. doi: 10.1016/0378-1119(80)90013-x. [DOI] [PubMed] [Google Scholar]
  3. Gergen J. P., Stern R. H., Wensink P. C. Filter replicas and permanent collections of recombinant DNA plasmids. Nucleic Acids Res. 1979 Dec 20;7(8):2115–2136. doi: 10.1093/nar/7.8.2115. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hunziker P. E., Hughes G. J., Wilson K. J. Peptide fragmentation suitable for solid-phase microsequencing. Use of N-bromosuccinimide and BNPS-skatole (3-bromo-3-methyl-2-[(2-nitrophenyl)thio]-3H-indole). Biochem J. 1980 May 1;187(2):515–519. doi: 10.1042/bj1870515. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Jones M. D., Petersen T. E., Nielsen K. M., Magnusson S., Sottrup-Jensen L., Gausing K., Clark B. F. The complete amino-acid sequence of elongation factor Tu from Escherichia coli. Eur J Biochem. 1980 Jul;108(2):507–526. doi: 10.1111/j.1432-1033.1980.tb04748.x. [DOI] [PubMed] [Google Scholar]
  6. Kaziro Y. The role of guanosine 5'-triphosphate in polypeptide chain elongation. Biochim Biophys Acta. 1978 Sep 21;505(1):95–127. doi: 10.1016/0304-4173(78)90009-5. [DOI] [PubMed] [Google Scholar]
  7. Laursen R. A., L'Italien J. J., Nagarkatti S., Miller D. L. The amino acid sequence of elongation factor Tu of Escherichia coli. The complete sequence. J Biol Chem. 1981 Aug 10;256(15):8102–8109. [PubMed] [Google Scholar]
  8. Leder P. The elongation reactions in protein synthesis. Adv Protein Chem. 1973;27:213–242. doi: 10.1016/s0065-3233(08)60448-9. [DOI] [PubMed] [Google Scholar]
  9. Liljas A. Structural studies of ribosomes. Prog Biophys Mol Biol. 1982;40(3):161–228. doi: 10.1016/0079-6107(82)90013-x. [DOI] [PubMed] [Google Scholar]
  10. Loeb L. A., Weymouth L. A., Kunkel T. A., Gopinathan K. P., Beckman R. A., Dube D. K. On the fidelity of DNA replication. Cold Spring Harb Symp Quant Biol. 1979;43(Pt 2):921–927. doi: 10.1101/sqb.1979.043.01.101. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Maxam A. M., Gilbert W. A new method for sequencing DNA. Proc Natl Acad Sci U S A. 1977 Feb;74(2):560–564. doi: 10.1073/pnas.74.2.560. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Montandon P. E., Stutz E. Nucleotide sequence of a Euglena gracilis chloroplast genome region coding for the elongation factor Tu; evidence for a spliced mRNA. Nucleic Acids Res. 1983 Sep 10;11(17):5877–5892. doi: 10.1093/nar/11.17.5877. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Nagata S., Tsunetsugu-Yokota Y., Naito A., Kaziro Y. Molecular cloning and sequence determination of the nuclear gene coding for mitochondrial elongation factor Tu of Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 1983 Oct;80(20):6192–6196. doi: 10.1073/pnas.80.20.6192. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Nakamura S., Nakayama N., Takahashi K., Kaziro Y. Primary structure of the polypeptide chain elongation factor Tu from E. coli. I. Amino acid sequence of fragment B. J Biochem. 1982 Mar;91(3):1047–1063. doi: 10.1093/oxfordjournals.jbchem.a133754. [DOI] [PubMed] [Google Scholar]
  15. Ovchinnikov YuA, Alakhov YuB, Bundulis YuP, Bundule M. A., Dovgas N. V., Kozlov V. P., Motuz L. P., Vinokurov L. M. The primary structure of elongation factor G from Escherichia coli. A complete amino acid sequence. FEBS Lett. 1982 Mar 8;139(1):130–135. doi: 10.1016/0014-5793(82)80503-6. [DOI] [PubMed] [Google Scholar]
  16. Ullrich A., Gray A., Berman C., Dull T. J. Human beta-nerve growth factor gene sequence highly homologous to that of mouse. Nature. 1983 Jun 30;303(5920):821–825. doi: 10.1038/303821a0. [DOI] [PubMed] [Google Scholar]
  17. Vieira J., Messing J. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene. 1982 Oct;19(3):259–268. doi: 10.1016/0378-1119(82)90015-4. [DOI] [PubMed] [Google Scholar]
  18. Volckaert G., Tavernier J., Derynck R., Devos R., Fiers W. Molecular mechanisms of nucleotide-sequence rearrangements in cDNA clones of human fibroblast interferon mRNA. Gene. 1981 Nov;15(2-3):215–223. doi: 10.1016/0378-1119(81)90131-1. [DOI] [PubMed] [Google Scholar]
  19. Yokota T., Sugisaki H., Takanami M., Kaziro Y. The nucleotide sequence of the cloned tufA gene of Escherichia coli. Gene. 1980 Dec;12(1-2):25–31. doi: 10.1016/0378-1119(80)90012-8. [DOI] [PubMed] [Google Scholar]
  20. Zimmern D., Kaesberg P. 3'-terminal nucleotide sequence of encephalomyocarditis virus RNA determined by reverse transcriptase and chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1978 Sep;75(9):4257–4261. doi: 10.1073/pnas.75.9.4257. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. van Hemert F. J., Lenstra J. A., Möller W. Genes for elongation factor EF-1 alpha in the brine shrimp Artemia. FEBS Lett. 1983 Jul 4;157(2):295–299. doi: 10.1016/0014-5793(83)80564-x. [DOI] [PubMed] [Google Scholar]
  22. van Hemert F. J., Roobol K., Möller W. Partial purification of the messenger RNA for eukaryotic elongation factor Tu from Artemia salina. Eur J Biochem. 1981 Nov;120(1):137–142. doi: 10.1111/j.1432-1033.1981.tb05680.x. [DOI] [PubMed] [Google Scholar]
  23. van Hemert F. J., van Ormondt H., Möller W. A bacterial clone carrying sequences coding for elongation factor EF-1 alpha from Artemia. FEBS Lett. 1983 Jul 4;157(2):289–293. doi: 10.1016/0014-5793(83)80563-8. [DOI] [PubMed] [Google Scholar]

Articles from The EMBO Journal are provided here courtesy of Nature Publishing Group

RESOURCES