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. 1984 Jul;3(7):1647–1654. doi: 10.1002/j.1460-2075.1984.tb02025.x

Eo: a history of a mutation.

Z Dembić, P A Singer, J Klein
PMCID: PMC557573  PMID: 6745246

Abstract

Eighteen mouse t haplotype-carrying strains were found not to express cell-surface E molecules controlled by class II genes of the H-2 complex (= Eo strains). Northern and Southern blot analysis of these and other, non-t strains that also fail to express the E molecule, has revealed two kinds of defect. Three strains (CRO437, tw2, and presumably to) were found to transcribe the E alpha gene, but they were not able to convert the message into a functional protein. All other Eo strains fail to transcribe the E alpha gene because of a deletion encompassing the promoter region, the RNA initiation site, and the first exon. The length of the deletion is approximately 650 +/- 50 bp. These two defects closely resemble those found previously in standard inbred strains carrying the H-2f, H-2q (failure of E mRNA to be expressed functionally), H-2b, and H-2s (deletion of a part of the E alpha gene) haplotypes. In particular, the location and length of the E alpha deletion appear to be the same in the strains carrying this mutation. The E alpha deletion is in linkage disequilibrium with certain alleles at other H-2 loci in some of the strains. These observations, combined with the growing evidence that H-2 haplotypes associated with t chromosomes derive from a single ancestral haplotype, suggest that the E alpha deletion is an old mutation and that it has been disseminated in mouse populations by the t chromosomes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Chirgwin J. M., Przybyla A. E., MacDonald R. J., Rutter W. J. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry. 1979 Nov 27;18(24):5294–5299. doi: 10.1021/bi00591a005. [DOI] [PubMed] [Google Scholar]
  2. Hyldig-Nielsen J. J., Schenning L., Hammerling U., Widmark E., Heldin E., Lind P., Servenius B., Lund T., Flavell R., Lee J. S. The complete nucleotide sequence of the I-E alpha d immune response gene. Nucleic Acids Res. 1983 Aug 11;11(15):5055–5071. doi: 10.1093/nar/11.15.5055. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Jones P. P., Murphy D. B., McDevitt H. O. Two-gene control of the expression of a murine Ia antigen. J Exp Med. 1978 Oct 1;148(4):925–939. doi: 10.1084/jem.148.4.925. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Jones P. P., Murphy D. B., McDevitt H. O. Variable synthesis and expression of E alpha and Ae (E beta) Ia polypeptide chains in mice of different H-2 haplotypes. Immunogenetics. 1981;12(3-4):321–337. doi: 10.1007/BF01561674. [DOI] [PubMed] [Google Scholar]
  5. Klein J., Figueroa F., David C. S. H-2 haplotypes, genes and antigens: second listing. II. The H-2 complex. Immunogenetics. 1983;17(6):553–596. doi: 10.1007/BF00366126. [DOI] [PubMed] [Google Scholar]
  6. Klein J., Figueroa F., Nagy Z. A. Genetics of the major histocompatibility complex: the final act. Annu Rev Immunol. 1983;1:119–142. doi: 10.1146/annurev.iy.01.040183.001003. [DOI] [PubMed] [Google Scholar]
  7. Klein J., Hammerberg C. The control of differentiation by the T complex. Immunol Rev. 1977 Jan;33:70–104. doi: 10.1111/j.1600-065x.1977.tb00363.x. [DOI] [PubMed] [Google Scholar]
  8. Mathis D. J., Benoist C., Williams V. E., 2nd, Kanter M., McDevitt H. O. Several mechanisms can account for defective E alpha gene expression in different mouse haplotypes. Proc Natl Acad Sci U S A. 1983 Jan;80(1):273–277. doi: 10.1073/pnas.80.1.273. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Nizetić D., Figueroa F., Klein J. Evolutionary relationships between the t and H-2 haplotypes in the house mouse. Immunogenetics. 1984;19(4):311–320. doi: 10.1007/BF00345404. [DOI] [PubMed] [Google Scholar]
  10. Rice M. C., O'Brien S. J. Genetic variance of laboratory outbred Swiss mice. Nature. 1980 Jan 10;283(5743):157–161. doi: 10.1038/283157a0. [DOI] [PubMed] [Google Scholar]
  11. Rogers J. H., Willison K. R. A major rearrangement in the H-2 complex of mouse t haplotypes. Nature. 1983 Aug 11;304(5926):549–552. doi: 10.1038/304549a0. [DOI] [PubMed] [Google Scholar]
  12. Shin H. S., Stavnezer J., Artzt K., Bennett D. Genetic structure and origin of t haplotypes of mice, analyzed with H-2 cDNA probes. Cell. 1982 Jul;29(3):969–976. doi: 10.1016/0092-8674(82)90460-3. [DOI] [PubMed] [Google Scholar]
  13. Silver L. M. Genomic analysis of the H-2 complex region associated with mouse t haplotypes. Cell. 1982 Jul;29(3):961–968. doi: 10.1016/0092-8674(82)90459-7. [DOI] [PubMed] [Google Scholar]
  14. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  15. Staats J. Standardized nomenclature for inbred strains of mice: seventh listing for the International Committee on Standardized Genetic Nomenclature for Mice. Cancer Res. 1980 Jul;40(7):2083–2128. [PubMed] [Google Scholar]
  16. Steinmetz M., Minard K., Horvath S., McNicholas J., Srelinger J., Wake C., Long E., Mach B., Hood L. A molecular map of the immune response region from the major histocompatibility complex of the mouse. Nature. 1982 Nov 4;300(5887):35–42. doi: 10.1038/300035a0. [DOI] [PubMed] [Google Scholar]
  17. Sturm S., Figueroa F., Klein J. The relationship between t and H-2 complexes in wild mice. I. The H-2 haplotypes of 20 t-bearing strains. Genet Res. 1982 Aug;40(1):73–88. doi: 10.1017/s0016672300018929. [DOI] [PubMed] [Google Scholar]
  18. Thomas P. S. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose. Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201–5205. doi: 10.1073/pnas.77.9.5201. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Zaleska-Rutczynska Z., Figueroa F., Klein J. Sixteen new H-2 haplotypes derived from wild mice. Immunogenetics. 1983;18(3):189–203. doi: 10.1007/BF00952959. [DOI] [PubMed] [Google Scholar]

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