Cell Biology. In the article “Quantitative analysis of biological membrane lipids at the low picomole level by nano-electrospray ionization tandem mass spectrometry” by B. Brügger, G. Erben, R. Sandhoff, F. T. Wieland, and W. D. Lehmann, which appeared in number 6, March 18, 1997, of Proc. Natl. Acad. Sci. USA (94, 2339–2344), Table 1 contains errors. A corrected table is reprinted below with corrections in boldface type.
Table 1.
Class of phospholipid | Ion | Total fatty acid carbon no.:no. of double bonds
|
||||||||
---|---|---|---|---|---|---|---|---|---|---|
32:1 | 34:2 | 36:2 | 38:4 | 16:0 | 18:0 | 22:0 | 24:1 | 26:0 | ||
PA | [M-H]− | 645 | 671 | 699 | 723 | |||||
PS | [M-H]− | 732 | 758 | 786 | 810 | |||||
PE | [M-H]− | 688 | 714 | 742 | 766 | |||||
Plasmenyl-PE | [M-H]− | 672 | 698 | 726 | 750 | |||||
PI | [M-H]− | 807 | 833 | 861 | 885 | |||||
PG | [M-H]− | 719 | 745 | 773 | 797 | |||||
PC | [M+H]+ | 732 | 758 | 786 | 810 | |||||
[M+Na]+ | 754 | 780 | 808 | 832 | ||||||
Plasmenyl-PC | [M+H]+ | 716 | 742 | 770 | 794 | |||||
[M+Na]+ | 738 | 764 | 792 | 816 | ||||||
PE | [M+H]+ | 690 | 716 | 744 | 768 | |||||
Plasmenyl-PE | [M+H]+ | 674 | 700 | 728 | 752 | |||||
PS | [M+H]+ | 734 | 760 | 788 | 812 | |||||
SM | [M+H]+ | 703 | 731 | 787 | 813 | 843 | ||||
[M+Na]+ | 725 | 753 | 809 | 835 | 865 |
In the negative ion mode, PS and PE (with two nitrogen atoms) show signals at even-numbered m/z values, whereas PI, PG, and PA show signals at odd-numbered values. In the positive ion mode, SM signals appear at odd m/z values (2 nitrogen atoms), whereas PC, PE, and PS-signals occur at even m/z values. The m/z values are nominal monoisotopic data.