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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1999 Sep 14;96(19):10943.
PMCID: PMC56170

Cell Biology. In the article “Quantitative analysis of biological membrane lipids at the low picomole level by nano-electrospray ionization tandem mass spectrometry” by B. Brügger, G. Erben, R. Sandhoff, F. T. Wieland, and W. D. Lehmann, which appeared in number 6, March 18, 1997, of Proc. Natl. Acad. Sci. USA (94, 2339–2344), Table 1 contains errors. A corrected table is reprinted below with corrections in boldface type.

Table 1.

Identification of the most abundant signals in the ESI mass spectra of total lipid extract of CHO cells, as given in Fig. 1 a and b

Class of phospholipid Ion Total fatty acid carbon no.:no. of double bonds
32:1 34:2 36:2 38:4 16:0 18:0 22:0 24:1 26:0
PA [M-H] 645 671 699 723
PS [M-H] 732 758 786 810
PE [M-H] 688 714 742 766
Plasmenyl-PE [M-H] 672 698 726 750
PI [M-H] 807 833 861 885
PG [M-H] 719 745 773 797
PC [M+H]+ 732 758 786 810
[M+Na]+ 754 780 808 832
Plasmenyl-PC [M+H]+ 716 742 770 794
[M+Na]+ 738 764 792 816
PE [M+H]+ 690 716 744 768
Plasmenyl-PE [M+H]+ 674 700 728 752
PS [M+H]+ 734 760 788 812
SM [M+H]+ 703 731 787 813 843
[M+Na]+ 725 753 809 835 865

In the negative ion mode, PS and PE (with two nitrogen atoms) show signals at even-numbered m/z values, whereas PI, PG, and PA show signals at odd-numbered values. In the positive ion mode, SM signals appear at odd m/z values (2 nitrogen atoms), whereas PC, PE, and PS-signals occur at even m/z values. The m/z values are nominal monoisotopic data. 


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