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. 2018 Feb 7;17:71. doi: 10.1186/s12936-018-2215-7

Population genetic structure and natural selection of Plasmodium falciparum apical membrane antigen-1 in Myanmar isolates

Jung-Mi Kang 1,2, Jinyoung Lee 1,6, Mya Moe 3, Hojong Jun 4, Hương Giang Lê 1,2, Tae Im Kim 1,7, Thị Lam Thái 1,2, Woon-Mok Sohn 1, Moe Kyaw Myint 3, Khin Lin 3, Ho-Joon Shin 5, Tong-Soo Kim 4, Byoung-Kuk Na 1,2,✉
PMCID: PMC5804060  PMID: 29415731

Abstract

Background

Plasmodium falciparum apical membrane antigen-1 (PfAMA-1) is one of leading blood stage malaria vaccine candidates. However, genetic variation and antigenic diversity identified in global PfAMA-1 are major hurdles in the development of an effective vaccine based on this antigen. In this study, genetic structure and the effect of natural selection of PfAMA-1 among Myanmar P. falciparum isolates were analysed.

Methods

Blood samples were collected from 58 Myanmar patients with falciparum malaria. Full-length PfAMA-1 gene was amplified by polymerase chain reaction and cloned into a TA cloning vector. PfAMA-1 sequence of each isolate was sequenced. Polymorphic characteristics and effect of natural selection were analysed with using DNASTAR, MEGA4, and DnaSP programs. Polymorphic nature and natural selection in 459 global PfAMA-1 were also analysed.

Results

Thirty-seven different haplotypes of PfAMA-1 were identified in 58 Myanmar P. falciparum isolates. Most amino acid changes identified in Myanmar PfAMA-1 were found in domains I and III. Overall patterns of amino acid changes in Myanmar PfAMA-1 were similar to those in global PfAMA-1. However, frequencies of amino acid changes differed by country. Novel amino acid changes in Myanmar PfAMA-1 were also identified. Evidences for natural selection and recombination event were observed in global PfAMA-1. Among 51 commonly identified amino acid changes in global PfAMA-1 sequences, 43 were found in predicted RBC-binding sites, B-cell epitopes, or IUR regions.

Conclusions

Myanmar PfAMA-1 showed similar patterns of nucleotide diversity and amino acid polymorphisms compared to those of global PfAMA-1. Balancing natural selection and intragenic recombination across PfAMA-1 are likely to play major roles in generating genetic diversity in global PfAMA-1. Most common amino acid changes in global PfAMA-1 were located in predicted B-cell epitopes where high levels of nucleotide diversity and balancing natural selection were found. These results highlight the strong selective pressure of host immunity on the PfAMA-1 gene. These results have significant implications in understanding the nature of Myanmar PfAMA-1 along with global PfAMA-1. They also provide useful information for the development of effective malaria vaccine based on this antigen.

Keywords: Plasmodium falciparum, Apical membrane antigen-1, Genetic diversity, Natural selection, Myanmar

Background

Global incidence and mortality of malaria have decreased in recent years, but malaria is still a great public health concern. In 2015, there were approximately 212 million clinical cases of malaria with an estimated 429,000 deaths [1]. The challenge for malaria control and elimination has increased due to the spread of parasites with resistance to anti-malarial drugs and insecticide-resistant Anopheles mosquitoes. The lack of effective vaccines is one of the major obstacles in combating malaria. Therefore, development of efficacious vaccine is urgently needed for malaria control. Up to date, several candidate proteins including circumsporozoite protein (CSP), Duffy-binding protein (DBP), merozoite surface protein-1 (MSP-1), apical membrane antigen-1 (AMA-1), and thrombospondin related anonymous protein (TRAP) have been tested for their potentials as candidate antigens to develop effective vaccines [2]. The majority of these antigens are expressed either in pre-erythrocytic and erythrocytic stages [2, 3]. However, genetic polymorphisms identified in these parasite antigens are great hurdles to develop effective vaccines since they generate a variant-specific immune response which is less effective against parasites with other genetic variants [4–6].

Apical membrane antigen-1 (AMA-1) is a 83-kDa type I integral membrane protein that is mainly expressed in the merozoite and sporozoite [7, 8]. Biological function of AMA-1 is not clearly understood yet, but its stage specific expression and localization suggest that the protein might play a crucial role in invasion of erythrocytes and hepatocytes by malaria parasites [8–11]. Structure analysis of AMA-1 revealed that this protein consists of a signal sequence, a cysteine-rich ectodomain, a conserved cytoplasmic region and a transmembrane region [12]. The ectodomain of AMA-1 is further subdivided into three domains, domains I, II, and III. The ectodomain AMA-1 is very immunogenic and natural immune responses against the domain have been reported in patients exposed to Plasmodium falciparum and Plasmodium vivax [13–16]. Immunization with AMA-1 can produce antibodies to effectively inhibit the invasion of erythrocyte by malaria parasite and confer protective immune responses [15, 17]. As a result, AMA-1 is a leading blood stage vaccine candidate for malaria control [16, 18, 19]. Several studies have shown that anti-AMA-1 antibodies confer protective immunity in adults living in malaria-endemic areas [20, 21]. However, these antibodies recognize either conserved or allele-specific epitopes of AMA-1, resulting limited protection against different alleles [22]. AMA-1 is known to be less variable than other malaria vaccine candidate antigens such as MSPs or CSP, but AMA-1 also possesses genetic diversity among global malaria parasites. A high rate of polymorphisms has been identified in domain I of AMA-1 and this region appears to be a major target of anti-AMA-1 protective antibodies [23–28]. To design an efficient and protective malaria vaccine, it is essential to monitor genetic variations of vaccine candidate antigens among global malaria isolates circulating in endemic areas [29].

The incidence of malaria in Myanmar has decreased in recent years. However, Myanmar still accounts for more than half of the malaria cases and approximately three quarters of the malaria deaths in the Greater Mekong Subregion [30]. Although P. falciparum cases have decreased in recent years [1, 31], this species is still the most critical concern for malaria control and prevention in Myanmar with the emergence of artemisinin resistance [32]. In this study, population genetic structure and natural selection of P. falciparum AMA-1 (PfAMA-1) in Myanmar P. falciparum isolates were analysed.

Methods

Blood samples and ethics

Blood samples used in this study were obtained from P. falciparum infected patients who resided in rural villages located in Naung Cho, Shan State, and Pyin Oo Lwin and Tha Beik Kyin, Mandalay Division, Upper Myanmar between July to December 2015 (Fig. 1). Malaria transmission was heterogeneous and seasonal in these areas and most malaria cases occurred with the peak during and just after the rainy season. The major ethnic groups in these regions were Bamar and Shan. Community-based survey was performed in 825 residents in 7 villages of the areas and P. falciparum infection was confirmed by Giemsa-stained thick and thin blood smear examination. The average age of patients was 34.7 years old ranged from 13 to 60 years. Two or three ml of venous blood was collected from the P. falciparum infected patients in EDTA tubes before drug treatment for further molecular analysis. All P. falciparum positive samples were further confirmed by polymerase chain reaction (PCR) targeting 18S ribosomal RNA (rRNA) gene [31, 33]. The use of blood samples in this study was approved by the Ministry of Health, Myanmar (Approval Number: 97/Ethics 2015). It was also approved by Biomedical Research Ethics Review Board of Inha University College of Medicine, Republic of Korea (Approval Number: INHA 15-013). Written consent was obtained from each individual prior to blood collection.

Fig. 1.

Fig. 1

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Map of the study sites. Community-based survey was performed in seven villages in Naung Cho, Shan State, and Pyin Oo Lwin and Tha Beik Kyin, Mandalay Division, Upper Myanmar between July to December 2015. Blood samples were collected from P. falciparum infected patients and used in this study

Amplification and sequencing analysis of PfAMA-1

Genomic DNA of parasite was extracted from 200 µl of whole blood sample of patients using QIAamp DNA Blood Kit (Qiagen, Hilden, Germany) following the manufacturer’s instruction. Full-length PfAMA-1 gene was amplified by PCR. PfAMA-1 gene-specific primers were designed based on complete coding sequences of PfAMA-1 of P. falciparum isolates 3D7 (GenBank Accession No.: U65407) and FC27 (GenBank Accession No.: M27133). Forward and reverse primers were 5′-GTACTTGTTATAAATTGTACA-3′ and 5′-TTTTAGCATAAAAGAGAAGC-3′, respectively. Thermal cycling parameters for PCR were as follows: one cycle of an initial denaturation at 94 °C for 5 min, 30 cycles of 94 °C for 1 min, annealing at 55 °C for 1 min and extension at 72 °C for 2 min, followed by a final extension step at 72 °C for 10 min. Nested PCR with primers 5′-ACAAAAATGAGAAAATTATACTGC-3′ and 5′-TTAATAGTATGGTTTTTCCATCAGAAC-3′ was performed with the same amplification condition, if necessary. To minimize nucleotide mis-incorporation into sequences during PCR amplification, Ex Taq DNA polymerase (Takara, Otsu, Japan) with proof-reading activity was used in all PCR. Each PCR product was analysed by electrophoresis on 1% agarose gels. The resulting PCR product was cut from the gel followed by gel purification. It was then cloned into T&A cloning vector (Real Biotech Corporation, Banqiao City, Taiwan). Each ligation mixture was transformed into Escherichia coli DH5α competent cells. Colony PCR was performed with the nested PCR primers to identify positive clones with appropriate insert. Nucleotide sequences of cloned PCR product were subjected to automatic DNA sequencing using M13 forward and M13 reverse primers. Sequencing was also carried out using two additional specific internal primers (5′-CAGGGAAATGTCCAGTATTTGGTA-3′ and 5′-TTCCATCGACCCATAATCCG-3′) to obtain clear sequences for central part of PfAMA-1. At least two clones from each isolate were sequenced to ensure sequencing accuracy. Some isolates underwent three or fourfold sequence coverage to confirm the existence of rare polymorphisms. These nucleotide sequences are deposited at GenBank under Accession Numbers KU893276–KU893333.

Nucleotide sequence polymorphism analysis and neutrality test

Nucleotide and deduced amino acid sequences of PfAMA-1 were analysed using EditSeq and SeqMan programs in DNASTAR package (DNASTAR, Madison, WI, USA). Nucleotide sequence polymorphism analysis was conducted for 58 PfAMA-1 sequences. Numbers of segregating sites (S), haplotypes (H), haplotype diversity (Hd), nucleotide diversity (π), and average number of pair-wise nucleotide differences within a population (K) were estimated using DnaSP ver. 5.10.00 [34]. Value of π was calculated to estimate step-wise diversity throughout the full-length PfAMA-1 based on a sliding window of 100 bases with a step size of 25 bp. Values of non-synonymous (dN) and synonymous (dS) substitutions were estimated and were compared using Z test (P < 0.05 was considered significant) in MEGA4 program [35] based on method of Nei and Gojobori [36] with Jukes and Cantor correction. The Tajima’s D value [37] and Fu and Li’s D and F values [38] were analysed using DnaSP ver. 5.10.00 to evaluate neutral theory of evolution [34]. Recombination parameter (R), which included the effective population size and probability of recombination between adjacent nucleotides per generation, and minimum number of recombination events (Rm) were analysed by DnaSP ver. 5.10.00 [34]. Linkage disequilibrium (LD) between different polymorphic sites was computed based on the R2 index using DnaSP ver. 5.10.00 [34].

Population diversity of PfAMA-1 among global P. falciparum isolates

Genetic diversity of PfAMA-1 in global P. falciparum isolates was analysed. Parasite populations from Ghana (n = 37, AB715698–AB715734), Papua New Guinea (PNG: n = 90, AB715870–AB715959), Philippines (n = 55, AB715815–AB715869), Solomon Islands (n = 50, AB715960–AB716009), Tanzania (n = 62, AB715636–AB715679), Thailand (n = 80, AB715735–AB715814), and Vanuatu (n = 85, AB716010–AB716094) were included in this analysis. These publically available sequences covered full-length sequences of PfAMA-1. Nucleotide sequence polymorphism analysis and neutrality test for each population were performed using DnaSP ver. 5.10.00 [34] and MEGA4 program [35] as described above. Genetic differentiation among parasite populations was calculated based on fixation index (Fst) to estimate pairwise DNA sequence diversity between and within populations using DnaSP ver. 5.10.01 [34]. To investigate relationships among PfAMA-1 haplotypes, the haplotype network for a total of 517 PfAMA-1 sequences including 58 Myanmar sequences and the 459 publically available sequences from Ghana, PNG, Philippines, Solomon Islands, Tanzania, Thailand, and Vanuatu was constructed using the program NETWORK version 5.0.0.3 with the Median Joining algorithm [39]. To assess whether genetic diversity in PfAMA-1 within P. falciparum isolates were associated with host’s immune pressure, genetic diversity in predicted B-cell epitopes, intrinsically unstructured/disordered regions (IUR), and RBC binding regions in global PfAMA-1 were analysed [40, 41]. Nucleotide diversity and natural selection of each region were also analysed using DnaSP ver. 5.10.00 [34], as described above.

Results

Sequence polymorphism in Myanmar PfAMA-1 sequences

Fifty-eight PfAMA-1 sequences with 37 different haplotypes were obtained from Myanmar P. falciparum isolates. Nucleotide sequence analysis of these 58 PfAMA-1 sequences compared to PfAMA-1from 3D7 (GenBank Accession Number: U65407) revealed 83 single nucleotide polymorphisms (SNPs) in Myanmar PfAMA-1 sequences, including 5 synonymous SNPs and 78 non-synonymous SNPs. These non-synonymous SNPs resulted in amino acid changes at 64 positions in 58 Myanmar PfAMA-1 sequences, including 56 di-morphic amino acid changes and 8 tri-, tetra-, or penta-morphic amino acid changes (Fig. 2). Most amino acid changes were identified in domain I (28 amino acid changes), domain II (11 amino acid changes), and domain III (11 amino acid changes). Although these amino acid changes were distributed throughout each Myanmar PfAMA-1 haplotype, three amino acid changes (R39H, Y175D, and P330S) were fixed in all Myanmar PfAMA-1 sequences (Fig. 2). Of the eight tri-, tetra-, or penta-morphic amino acid changes, seven (E187K/N, E197Q/G/H/D, H200L/D/R, F201L/S/V, M243N/E, Q285E/P, and K300E/N) were found in domain I while the other one (Q407H/R) was found in domain II. Of the 56 di-morphic amino acid changes identified in Myanmar PfAMA-1, 44 have been previously reported in PfAMA-1 from other geographical P. falciparum isolates. However, the remaining 12 changes (S35C, N297T, V341A, Q349H, K351Q, Q355P, E449A, R456S, K472N, V497I, S500G, and Y565S) are novel that have not been reported previously.

Fig. 2.

Fig. 2

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Amino acid sequence polymorphism of the PfAMA-1 in Myanmar Plasmodium falciparum isolates. Thirty-seven haplotypes of Myanmar PfAMA-1 classified by nucleotide sequences were further classified into 36 distinct haplotypes based on amino acid sequences. Amino acid sequences of 36 haplotypes of Myanmar PfAMA-1 were compared to PfAMA-1 of 3D7 (GenBank Accession No.: U65407). Identical amino acid residues are indicated by dots. A total of 78 non-synonymous SNPs identified in 58 Myanmar PfAMA-1 resulted in amino acid changes at 64 positions. These amino acid changes were not evenly distributed in each PfAMA-1 haplotype. However, sequences clustered into an individual haplotype shared common amino acid changes. Conserved amino acid changes identified in all Myanmar PfAMA-1 sequences are presented as bold red with yellow background. Tri-morphic amino acid changes are presented as bold blue. Tetra- or penta-morphic amino acid changes are marked as bold purple. Rare amino acid changes identified are marked in bold green

Amino acid polymorphisms in Myanmar PfAMA-1 compared to global PfAMA-1

When patterns of amino acid polymorphisms identified in Myanmar PfAMA-1 were compared to those of PfAMA-1 from other countries, similar but not identical polymorphic patterns were observed. Most amino acid changes identified in global PfAMA-1 were found in domains I and III. Amino acid changes were also found in domain II, 5ʹ-terminal (5ʹ-T), and 3ʹ-terminal (3ʹ-T) regions (Fig. 3). Overall patterns of amino acid changes observed in global PfAMA-1 were similar to each other. However, frequencies of each amino acid change differed by country. The most notable amino acid changes identified in Myanmar PfAMA-1 were that three amino acid changes (R39H, Y175D and P330S) were fixed in all Myanmar PfAMA-1 sequences, even though their frequencies were also high in PfAMA-1 of other global isolates. Frequencies of R39H variation in other global isolates were as follow: Thailand, 100%; Philippines, 85.5%; PNG, 90.0%; Solomon Islands, 100%; Vanuatu, 95.3%; Ghana, 86.5%; Tanzania, 75.8%. The frequency of Y175D variation was also identified high in PfAMA-1 sequences from Thailand (87.5%), Philippines (90.9%), PNG (86.7%), Solomon Islands (76.0%), Ghana, (94.6%), and Tanzania, (95.2%) except for Vanuatu (52.9%). Substitution rate of P330S was also high in PfAMA-1 from all countries: Thailand, 100%; Philippines, 85.5%; PNG, 93.3%; Solomon Islands, 100%; Vanuatu, 100%; Ghana 83.8%; Tanzania 83.9%. High frequencies of N34K (86.2%), E52Q/K (62.1%), G172E (63.8%), E187K/N (75.8%), E197Q/G/D/H (81.1%), I332N (82.8%), and D584H (70.7%) variations were also observed in Myanmar PfAMA-1. These seven amino acid changes also showed high level of polymorphic patterns in global PfAMA-1 sequences. However, their frequencies varied by country. Meanwhile, S35C, N297T, V341A, Q349H, K351Q, Q355P, E449A, R456S, K472N, V497I, S500G, and Y565S were uniquely identified in Myanmar PfAMA-1, although their substitution frequencies were very low. Six amino acid changes (E50K, D69V, E121K, R199K, N228K, and E328K) were common in African PfAMA-1 sequences (Ghana and Tanzania). They were not identified or very rarely identified in PfAMA-1 sequences from other countries.

Fig. 3.

Fig. 3

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Comparison of amino acid polymorphisms of PfAMA-1 among global Plasmodium falciparum isolates. Positions and frequencies of amino acid changes found in PfAMA-1 of global P. falciparum isolates are compared. Each domain is represented by different color; Domain I (pink), domain II (yellow), and domain III (sky blue)

Nucleotide diversity and natural selection of Myanmar PfAMA-1

Nucleotide diversity and natural selection of PfAMA-1 of these 58 Myanmar P. falciparum isolates were analysed. The K value for full-length PfAMA-1 of Myanmar samples was 19.886 (Table 1). The highest nucleotide differences was found at domain I (K = 9.796) while the lowest was found at 5ʹ-T (K = 1.253). Haplotype diversity for full-length PfAMA-1 sequences of these 58 isolates was 0.948 ± 0.019. This value was higher at domain I (0.912 ± 0.024) than that at domains III (0.871 ± 0.024) or domain II (0.860 ± 0.023). The π value of the full-length PfAMA-1 for Myanmar isolates was 0.01064 ± 0.00051. Analysis of the π values for the 5ʹ-T, domain I, domain II, domain III, and 3ʹ-T revealed that most nucleotide diversity was concentrated at domains I and III. The π value for each fragment was: 0.00282 ± 0.00027 for 5ʹ-T, 0.02120 ± 0.00161 for domain I, 0.00752 ± 0.00057 for domain II, 0.01629 ± 0.00080 for domain III, and 0.00581 ± 0.00047 for 3ʹ-T (Table 1). To examine whether natural selection might have contributed to generation of PfAMA-1 diversity in Myanmar P. falciparum population, the value of dN − dS was estimated using the Nei and Gojobori’s method [36]. The value of dN − dS for full-length PfAMA-1 was 0.01076, suggesting that balancing natural selection might have occurred in the PfAMA-1 of Myanmar P. falciparum population. Considering high positive dN − dS values for domains I (0.02516) and III (0.01484), these domains might be main regions under balancing natural selection forces. The estimated Tajima’s D was 0.57189 (P > 0.10) (Table 1). When Tajima’s D value was analysed for each domain, domain I and domain III showed higher positive Tajima’s D values compared to other domains.

Table 1.

Estimates of DNA sequence polymorphism and tests of neutrality at PfAMA-1 among Plasmodium falciparum Myanmar isolates

Fragment Segregating sites (S) Singleton variable sites Parsimony informative sites Total no. of mutations K H Hd ± SD π ± SD dN − dS Tajima’s D
5ʹ-Terminal 5 1 4 5 1.253 8 0.702 ± 0.052 0.00282 ± 0.00027 − 0.00227 0.37596
(P > 0.1)
Domain I 37 4 33 38 9.796 27 0.912 ± 0.024 0.02120 ± 0.00161 0.02516 0.64221
(P > 0.1)
Domain II 12 4 8 12 2.234 12 0.860 ± 0.023 0.00752 ± 0.00057 0.00794 − 0.39855
(P > 0.1)
Domain III 13 5 8 13 3.275 14 0.871 ± 0.024 0.01629 ± 0.00080 0.01484 0.48627
(P > 0.1)
3ʹ-Terminal 8 2 6 8 1.987 10 0.761 ± 0.027 0.00581 ± 0.00047 0.00658 0.39702
(P > 0.1)
Full 78 16 62 79 19.886 37 0.948 ± 0.019 0.01064 ± 0.00051 0.01076 0.57189
(P > 0.1)
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K, average number of pairwise nucleotide differences; H, number of haplotypes; Hd, haplotype diversity; π, observed average pairwise nucleotide diversity; dN, rate of non-synonymous mutations; dS, rate of synonymous mutations

Nucleotide diversity and natural selection of PfAMA-1 in global isolates

Nucleotide diversity of PfAMA-1 among global isolates, including Ghana, PNG, Philippines, Solomon Islands, Tanzania, Thailand, and Vanuatu, were analysed and compared to Myanmar PfAMA-1. K values in African PfAMA-1 (Ghana, K = 26.261; Tanzania, K = 25.444) were higher than those of Asian and Pacific PfAMA-1. Nucleotide diversity across the full-length PfAMA-1 from different geographical areas also slightly differed by geographical areas (Table 2). The level of nucleotide diversity across PfAMA-1 in Myanmar P. falciparum isolates (π = 0.01064) was lower than that for PfAMA-1 from Ghana (π = 0.01405), PNG (π = 0.01226), Tanzania (π = 0.01361), or Solomon Islands (π = 0.01179), but similar to that for PfAMA-1 from Thailand (π = 0.01103), Philippines (π = 0.01136), or Vanuatu (π = 0.01012). A sliding window plot of π revealed that PfAMA-1 from different geographical areas shared highly similar patterns of nucleotide diversity through their sequences with two peaks at domains I and III. The maximum diversity was found at domain I (Fig. 4a). All PfAMA-1 sequences from different countries showed positive Tajima’s D values, suggesting that balancing selection of global PfAMA-1 (Table 2). A sliding window plot analysis also showed that global PfAMA-1 had similar pattern for Tajima’s D across the gene, albeit some differences were identified among PfAMA-1 with different geographical origins (Fig. 4b).

Table 2.

Estimates of DNA sequence polymorphism and tests of neutrality at PfAMA-1 among global Plasmodium falciparum

Isolates Segregating sites (S) Singleton variable sites Parsimony informative sites Total no. of mutations K H Hd ± SD π ± SD dN/dS Tajima’s D Fu and Li’s D Fu and Li’s F
Myanmar
(n = 58)		 78 16 62 79 19.886 37 0.948 ± 0.019 0.01064 ± 0.00053 6.524 0.57189
(P > 0.1)		 0.16773
(P > 0.1)		 0.38506
(P > 0.1)
Thailand
(n = 80)		 58 5 53 61 20.621 21 0.920 ± 0.013 0.01103 ± 0.00020 6.698 2.22215
(P < 0.05)		 0.95723
(P > 0.1)		 1.75160
(P < 0.05)
Philippines
(n = 55)		 61 3 58 62 21.232 19 0.916 ± 0.019 0.01136 ± 0.00027 7.200 1.95452
(0.1 > P > 0.05)		 1.61226
(P < 0.05)		 2.07959
(P < 0.02)
PNG
(n = 90)		 68 2 66 75 22.923 28 0.954 ± 0.007 0.01226 ± 0.00016 7.497 1.80824
(0.1 > P > 0.05)		 1.67585
(P < 0.05)		 2.07602
(P < 0.02)
Solomon Islands
(n = 50)		 56 3 53 57 22.044 9 0.862 ± 0.021 0.01179 ± 0.00027 7.023 2.54552
(P < 0.05)		 1.54470
(P < 0.05)		 2.28042
(P < 0.02)
Vanuatu
(n = 85)		 50 6 44 50 18.905 5 0.633 ± 0.028 0.01012 ± 0.00031 6.471 2.89729
(P < 0.01)		 0.86419
(P > 0.1)		 2.00393
(P < 0.05)
Ghana
(n = 37)		 75 7 68 85 26.261 32 0.992 ± 0.008 0.01405 ± 0.00034 7.423 1.06738
(P > 0.1)		 1.21791
(P > 0.1)		 1.38496
(P > 0.1)
Tanzania
(n = 62)		 81 8 73 89 25.444 47 0.989 ± 0.005 0.01361 ± 0.00021 8.135 1.18188
(P > 0.1)		 1.16354
(P > 0.1)		 1.40203
(P > 0.1)
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K, average number of pairwise nucleotide differences; H, number of haplotypes; Hd, haplotype diversity; π, observed average pairwise nucleotide diversity; dN, rate of non-synonymous mutations; dS, rate of synonymous mutations

Fig. 4.

Fig. 4

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Nucleotide diversity and natural selection of global PfAMA-1. a Nucleotide diversity. Sliding window plot analysis showed nucleotide diversity (π) values across global PfAMA-1 sequences analysed. A window size of 100 bp and a step size of 25 bp were used. b Natural selection. Sliding window calculation of Tajima’s D statistic was performed for global PfAMA-1. A window size of 100 and a step size of 25 were used. Myanmar, red line; Ghana, blue line; Papua New Guinea (PNG), yellow line; Philippines, green line; Solomon Islands (SI), purple; Tanzania, dotted red line; Thailand, dotted blue line; and Vanuatu, black line

Recombination and linkage disequilibrium

The minimum number of recombination events between adjacent polymorphic sites (Rm) for Myanmar PfAMA-1 was estimated to be 24. The R values between adjacent sites (Ra) and per gene (Rb) were 0.0141 and 26.3, respectively. Possible recombination events were also identified in global PfAMA-1. The highest R values were predicted for African PfAMA-1 (Ghana and Tanzania), while the lowest R values were predicted for Vanuatu PfAMA-1 (Table 3). LD index (R2) for global PfAMA-1 also decreased with increasing distance across the gene (Fig. 5).

Table 3.

Comparison of recombination events between global PfAMA-1

Ra Rb Rm
Myanmar 0.0141 26.3 24
Ghana 0.0921 172 27
PNG 0.0500 83.4 28
Philippines 0.0218 40.8 17
Solomon Islands 0.0120 22.5 14
Tanzania 0.0915 171 28
Thailand 0.0243 45.3 20
Vanuatu 0.0010 1.8 9
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The R and Rm were estimated excluding the sites containing alignment gaps or those segregating for three nucleotides. The R was computed using R = 4Nr, where N is the population size and r is the recombination rate per sequence (per gene) n, number of isolates; Ra, recombination parameter between adjacent sites; Rb, recombination parameter for entire gene; Rm, minimum number of recombination events between adjacent sites

Fig. 5.

Fig. 5

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Recombination event in global PfAMA-1. Linkage disequilibrium (LD) plot showed non-random associations between nucleotide variants in PfAMA-1 at different polymorphic sites. R2 values were plotted against nucleotide distance using a two-tailed Fisher’s exact test for statistical significance. PNG Papua New Guinea, SI Solomon Islands

Haplotype network analysis

Haplotype network analysis of PfAMA-1 haplotypes from global P. falciparum populations showed a dense network with extremely complex relationships (Fig. 6). A total of 174 distinct haplotypes were identified in 517 PfAMA-1 sequences analysed, of which 33.9% was singleton. Haplotype prevalence ranged from 0.19 to 13.7%. The most prevalent haplotype was haplotype 57 (H57) with a frequency of 13.7%. This haplotype was the only one shared by populations from six different Asian and Pacific countries (Myanmar, Thailand, Philippines, PNG, Solomon Islands, and Vanuatu). Haplotype 72 (H72) was another major haplotype with a high prevalence (13.1%) and was composed with Pacific populations. Four haplotypes, H71, H73, H79, and H112, were also admixed ones by Pacific populations. Meanwhile, two haplotypes, H5 and H16, were composed solely of African populations, Ghana and Tanzania. Only four haplotypes, H47, H76, H97, and H111, were haplotypes of African populations mixed with Asian or Pacific populations. Haplotype with sequence identical to 3D7 (GenBank Accession No.: U65407) and FVO (GenBank Accession No.: AJ277646) was H7 and H57, respectively.

Fig. 6.

Fig. 6

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Network analysis of global PfAMA-1 haplotypes. Haplotype network was constructed using the program NETWORK version 5.0.0.3 with the Median Joining algorithm. A total of 517 PfAMA-1 sequences were analysed. The size of each node reflected the frequency of a particular haplotype. The lengths of the lines connecting the nodes, measured from their centers, were in proportion to the number of base pair substitutions separating the haplotypes. Color of each node indicated different country

Nucleotide differentiation among global PfAMA-1

To investigate the degree of gene flow and genetic differentiation among global PfAMA-1 sequences, Fst values were evaluated for full-length PfAMA-1 sequences from different geographical P. falciparum populations deposited at GenBank (Table 4). Fst values between different geographical PfAMA-1 populations varied from 0.00019 (between Ghana and Tanzania) to 0.14141 (between Vanuatu and Tanzania).

Table 4.

Pairwise FST estimates for PfAMA-1

Myanmar Thailand Philippines PNG Solomon Islands Vanuatu Ghana Tanzania
Myanmar 20.15798 20.35295 21.58878 20.68805 19.15425 22.14497 20.15798
Thailand 0.06073 20.87018 21.83959 21.16846 19.73726 22.40481 22.72679
Philippines 0.08390 0.03818 22.28146 21.61888 19.81950 23.25483 23.46398
PNG 0.05281 0.03818 0.02319 22.60884 20.97135 23.89528 23.95093
Solomon Islands 0.09605 0.06636 0.03529 0.03529 20.06783 23.83759 23.92600
Vanuatu 0.09740 0.10535 0.06689 0.06689 0.04933 21.13622 21.66300
Ghana 0.07802 0.04140 0.04167 0.03640 0.04953 0.12425 25.74924
Tanzania 0.08139 0.05865 0.05787 0.05787 0.05872 0.14141 0.00019
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Fst values are shown in the lower left quadrant and average number of pair-wise nucleotide differences between populations (K) are shown in the upper right quadrant. Fst, a measure of genetic differentiation between populations (range from 0 to + 1)

Association between natural selection and host immune pressure

The selective pressure of host immunity on PfAMA-1 was evaluated by analysing genetic polymorphisms in predicted RBC-binding sites, B-cell epitopes and IUR regions. Results showed that most amino acid changes caused by SNPs were found in predicted RBC-binding sites, B-cell epitopes, or IUR regions of PfAMA-1 (Fig. 7a). Of 83 amino acid changes, 66 were found in global PfAMA-1 compared to PfAMA-1 of 3D7 (GenBank Accession No.: U65407) at predicted RBC-binding sites, B-cell epitopes, or IUR regions. Among 51 commonly identified amino acid changes in global PfAMA-1, 43 were found in predicted RBC-binding sites, B-cell epitopes, or IUR regions. The 23 less commonly changed amino acids in global PfAMA-1 sequences were also found in predicted RBC-binding sites, B-cell epitopes, or IUR regions. Eight of 11 predicted B-cell epitopes were polymorphic. In particular, high levels of π were predicted for B-cell epitopes 3, 4, 5, 8, and 10. These regions contained major polymorphic amino acid residues in global PfAMA-1 (Fig. 7b). Tajima’s D values for the predicted B-cell epitopes 3, 4, and 8 were all positive, indicating a decrease in population size and/or balancing selection. Meanwhile, Tajima’s D values for the predicted B-cell epitopes 5 and 10 were negative (Fig. 7b). Common amino acid changes found in global PfAMA-1 were located at C1-L covering region, which is located near the hydrophobic pocket of PfAMA-1. Meanwhile, only a few less frequent amino acid changes were identified in loop II of PfAMA-1.

Fig. 7.

Fig. 7

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Association between natural selection and host immune pressure. a Positions of amino acid changes found in global PfAMA-1 and predicted RBC-binding regions, B-cell epitopes, and IUR regions. Predicted RBC-binding regions [41], B-cell epitopes, and IUR regions [40] are presented by black underlines, dotted red lines, and bold blue lines, respectively. Polymorphic amino acid residues commonly identified in global PfAMA-1 are marked as bold red with underline. The less commonly identified amino acid changes are shown as bold blue. C1-L (cluster 1 of loop I, aa: 196–207) in DI and loop II (aa: 348–392) in DII are shown in yellow and sky blue squares, respectively. b Nucleotide diversity and natural selection analysis. Nucleotide diversity (π) and Tajima’s D (TD) values for each RBC-binding region, B-cell epitope region, and IUR region in global PfAMA-1 were analysed using DnaSP program. PNG Papua New Guinea, SI Solomon Islands

Discussion

Population genetic structure and natural selection of PfAMA-1 among Myanmar P. falciparum isolates were analysed in this study. Most of amino acid changes identified in Myanmar PfAMA-1 were clustered at domains I and III, consistent with previous reports on PfAMA-1 from other endemic areas [40, 42–45]. Overall distribution patterns and frequencies of amino acid changes found in Myanmar PfAMA-1 were similar to those of global PfAMA-1 analysed in this study, but several differences among and between global PfAMA-1 were also identified. The implication of these substantial geographic differentiations is currently unclear. Considering that only a limited number of PfAMA-1 sequences in each geographical area were analysed in this study, these amino acid changes and their different frequencies may not be statistically significant. Indeed, many amino acid changes found in global PfAMA-1 were commonly detected and evenly distributed among global PfAMA-1 sequences, although their frequencies differed among and between populations. Further examination of PfAMA-1 nucleotide and amino acid variations in diverse PfAMA-1 populations with a larger number of global PfAMA-1 sequences would be necessary to better understand the polymorphic nature of PfAMA-1. It is also worthy to mention that Myanmar PfAMA-1 sequences analysed in this study were from P. falciparum isolates that collected in restricted areas of Myanmar. Therefore, nation-wide analysis of PfAMA-1 in P. falciparum isolates collected from various regions of Myanmar is also needed to clearly understand the genetic diversity and population structure of Myanmar PfAMA-1.

The π value for global PfAMA-1 varied, ranging from 0.01012 (Vanuatu) to 0.01405 (Ghana). The π values in African PfAMA-1 sequences (Ghana and Tanzania) were relatively higher than those in Asian or Pacific PfAMA-1 sequences. Nucleotide diversity was not evenly distributed through Myanmar PfAMA-1. The 5ʹ-T, domain II and 3ʹ-T showed low levels of nucleotide diversity, indicating that these regions might be more conserved with lower frequency of polymorphisms. Meanwhile, much higher value of nucleotide diversity was observed at domain I and domain III. Highly similar patterns of nucleotide diversity were also detected in global PfAMA-1 analysed in this study, strongly indicating that global PfAMA-1 might share highly similar nucleotide diversity across this gene. The dN − dS value for Myanmar full-length PfAMA-1 was positive, implying balancing selection in this gene. However, the dN − dS value for 5ʹ-T was negative, suggesting negative or purifying natural selection in this domain. Tajima’s D value (the statistic value predicting departure from neutrality) for Myanmar PfAMA-1 also suggested that this gene might have evolved under balancing selection. In sliding plot analysis of Tajima’s D, general patterns of Tajima’s D values across Myanmar PfAMA-1 were similar to those of global PfAMA-1, although differences among and between PfAMA-1 from different countries were also found. Regardless of theses slight differences, domains I and III and the junction between domains II and III shared very similar patterns of Tajima’s D values in global PfAMA-1, indicating that these regions might be dominant targets for host immune response. Fu and Li’s D and F tests also provided evidence for balancing selection in global PfAMA-1. These results collectively suggest that domains I and III of Myanmar PfAMA-1 are highly polymorphic with strong selection force acting on these domains, similar to other global PfAMA-1. Meiotic recombination between the adjacent polymorphic sites is one of main forces that drive allelic diversity in Plasmodium parasites [40]. Substantial levels of recombination events in PfAMA-1 from different geographical isolates have been reported previously [40, 43, 45–49]. Recombination events within Myanmar PfAMA-1 and decline of LD index R2 with the increase of nucleotide distance of the sequences evidenced meiotic recombination in Myanmar PfAMA-1, consistent with previous studies [40, 43, 45–49]. Higher values of recombination events were found in African PfAMA-1 sequences (Ghana and Tanzania) than those of PfAMA-1 from other geographical areas, suggesting that African PfAMA-1 might have more opportunity to have recombination events than other geographical PfAMA-1 probably due to high multiclonal infections, subsequent cross fertilization, and recombination in mosquitoes. High values of recombination parameters and very rapid decay of LD detected in global PfAMA-1 are independent and concordant indicators of high meiotic recombination rate in global PfAMA-1, supporting the notion that recombination is another important factor generating genetic diversity in global PfAMA-1.

Fst is a measure of population substructure and is one of the most useful tools for analysing the overall genetic differentiation among populations. Fst values at each locus are considered as no differentiation (0), low genetic differentiation (0–0.05), moderate differentiation (0.05–0.15), or high differentiation (0.15–0.25) [50]. Fst values for Myanmar PfAMA-1 in comparison with PfAMA-1 from other different geographical populations showed moderate levels of genetic differentiation, ranging from 0.058281 to 0.09740. Fst values between PfAMA-1 populations belonging to the same geographical area were relatively low. In particular, the value between Ghana and Tanzania was very low (0.00019), suggesting very low genetic differentiation among these two populations. However, Vanuatu showed relatively high Fst values for Ghana (0.12425) and Tanzania (0.14141), providing evidence for geographical isolation followed by population subdivision in these regions. Overall Fst values between global PfAMA-1 were in moderate differentiation ranges, indicating limited genetic differentiation of PfAMA-1 among global parasite populations.

The significant ratio of dN/dS and evidence of positive natural selection identified in PfAMA-1 of Myanmar and the other seven P. falciparum populations suggest that amino acid replacements are generally favored in PfAMA-1 and this adaptive evolution is presumably due to host immune pressure. Previous studies have suggested that polymorphic amino acid residues identified in PfAMA-1 are located on one side of the molecule, thereby exposing them to the exterior environment more easily with more access to host immune responses [40, 43, 51]. A recent study has provided information on potential RBC-binding regions, B-cell epitopes, and IURs across the ectodomain of PfAMA-1 [40]. To assess the association between natural selection and host immune pressure for PfAMA-1, genetic polymorphisms in predicted RBC-binding sites, B-cell epitopes, and IUR regions were analysed. Most amino acid changes found in global PfAMA-1 were predicted to be localized at the predicted RBC-binding sites, B-cell epitopes, or IUR regions of PfAMA-1. Eight of 11 predicted B-cell epitopes were polymorphic. In particular, B-cell epitopes 3, 4, 5, 8, and 10 (corresponding to domain I, junction between domain II and III, and domain III) showed high levels of nucleotide diversity in global PfAMA-1 analysed. Tajima’s D values for these predicted B-cells epitopes also suggested that these epitopes were under natural selection. The C1-L cluster, which located near the hydrophobic pocket of domain I in PfAMA-1, affects binding capacity of inhibitory monoclonal antibody, thereby contributing to antibody escape [52–55]. Global PfAMA-1 analysed in this study had clustering amino acid polymorphisms, including tri- and hepta-morphic changes in C1-L region. This supports the notion that strong natural selection force which generates genetic diversity of PfAMA-1, thereby contributing to host immune escape acts on this region [21, 54, 55]. Meanwhile, only a few less frequent dimorphic amino acid changes were identified in loop II of global PfAMA-1, the target of 4G2 inhibitory antibody [56], coinciding with results of a previous study [40]. High degree of amino acid sequence conservation of the loop II among global PfAMA-1 further supports the hypothesis that amino acid sequences in this region are subjected to strong natural selection. Therefore, this region might be useful as a component of PfAMA-1-based vaccine.

To develop a globally effective malaria vaccine based on PfAMA-1, it would be important to understand whether PfAMA-1 haplotypes with distinct clusters are antigenically different. Haplotype network analysis of 517 global PfAMA-1 sequences suggested that a total of 174 distinct haplotypes were identified among global population. No haplotype that fully covers haplotypes from all geographical areas analyzed in this study was identified. PfAMA-1 based vaccines currently studied are based on sequences of most extensively studied forms of P. falciparum, 3D7 and FVO [57–59]. However, only 13.7% of global PfAMA-1 haplotypes analysed in this study was identical to the FVO. The frequency of global PfAMA-1 haplotypes with identical sequence to 3D7 was much lower. These raised a concern that vaccine based on these two forms of haplotypes may not offer effective protection for global P. falciparum isolates. A recent report suggested that PfAMA-1 sequence variations may not necessarily be strong predictors of antigenic differences or the level of cross-inhibitory antibody activity because not all polymorphic residues contribute equally to antibody binding and escape [60]. Antigenic diversity of PfAMA-1 is limited and a vaccine targeting a small number of PfAMA-1 alleles might be sufficient for coverage against naturally-circulating P. falciparum populations in different endemic areas [27]. The results of this study also suggested that the genetic diversity is relatively limited among global PfAMA-1, even though substantial geographic differentiations were also identified among the populations. However, global PfAMA-1 is under natural selection and high level of meiotic recombination that can produce new alleles occurs in the gene population. And therefore, a multi-allele approach for developing PfAMA-1 based malaria vaccines should be considered to maximize vaccine efficacy.

Conclusions

Overall patterns of nucleotide diversity and distributions of amino acid changes found in Myanmar PfAMA-1 were similar to those from global PfAMA-1, albeit several amino acid changes were identified only in Myanmar PfAMA-1. Balancing natural selection across PfAMA-1 and high degree of recombination events observed in Myanmar and global PfAMA-1 suggest that natural selection and intragenic recombination might be main driving forces that generate genetic diversity in global PfAMA-1. Most common amino acid changes found in global PfAMA-1 were located at predicted B-cell epitopes. High degree of nucleotide diversity and balancing natural selection of the B-cell epitope regions suggest that strong natural selection might have act on epitopes associated with host immune pressure for PfAMA-1. Limited genetic differentiation of PfAMA-1 was estimated between and among global parasite populations, even though highly complicated haplotype diversity was identified in global population. However, global PfAMA-1 is under natural selection and high level of meiotic recombination that can produce new alleles occurs in the gene population.

The results of this study warrant continuous examination of PfAMA-1 nucleotide and amino acid variations in global PfAMA-1 to better understand the polymorphic nature of PfAMA-1 and design effective vaccine against global P. falciparum populations.

Authors’ contributions

JMK and JL carried out genetic analysis of PfAMA-1. MM, HJ, MKM and KL contributed the blood sample collection. JMK, JL, TIK, TLT, HGL, WMS, TSK and BKN analysed and interpreted the data. TSK and BKN designed and supervised the experiments. JMK, TLT, HGL and BKN wrote the draft of the manuscript. All authors read and approved the final manuscript.

Acknowledgements

We thank the staffs in Department of Medical Research Pyin Oo Lwin Branch and the health professionals in Naung Cho, Pyin Oo Lwin and Tha Beik Kyin townships for their contribution and technical support in field study.

Competing interests

The authors declare that they have no competing interests.

Availability of data and materials

The original datasets analysed in the current study are available from the corresponding author upon request.

Consent for publication

All authors have given their consent for publication.

Ethics approval and consent to participate

This study was approved by the Ethics Review Committee, Department of Medical Research, Myanmar (97/Ethics 2015), and by the Ethical Review Committee of Inha University School of Medicine, Korea (INHA 15-013). Informed written consent and permission were obtained from each individual.

Funding

This work was supported by the National Research Foundation of Korea (NRF) grants funded by the Korea government (MSIP) (NRF-2015R1A2A2A01004310) and the Korea government (MEST) (NRF-2015K1A3A9A01034893).

Publisher’s Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

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Associated Data

This section collects any data citations, data availability statements, or supplementary materials included in this article.

Data Availability Statement

The original datasets analysed in the current study are available from the corresponding author upon request.

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