Skip to main content
PMC home page
Japanese Journal of Cancer Research : Gann logoLink to Japanese Journal of Cancer Research : Gann
. 1996 Jul;87(7):680–684. doi: 10.1111/j.1349-7006.1996.tb00277.x

Prevention of Mammary Tumorigenesis in Acatalasemic Mice by Vitamin E Supplementation

Kunihiko Ishii 1,✉, Li‐Xue Zhen 1, Da‐hong Wang 1, Yuka Funamori 1, Katsuo Ogawa 2, Kazuhisa Taketa 1
PMCID: PMC5921155  PMID: 8698615

Abstract

Adult male and female acatalasemic (C3H/AnLCsbCsb), hypocatalasemic (C3H/AnLCscCsc) and normal mice of C3H strain fed on regular laboratory chow for 15 months showed an increased incidence of spontaneous mammary tumor in the decreasing order of female acatalasemic, male acatalasemic, female hypocatalasemic and male hypocatalasemic mice. Normal mice did not develop mammary tumor. We conducted a prospective study with female acatalasemic mice, which showed the highest incidence of mammary tumor, to examine the preventive effect of vitamin E on mammary tumor. Female acatalasemic mice were fed on vitamin E‐deficient (28 animals) and vitamin E‐supplemented diet (25 animals) for 29 months. The incidence of mammary tumor in mice given the vitamin E‐supplemented diet was 47%, while that in mice given vitamin E‐deficient diet was 82% (P<0.002). Mammary tumors were apparent after 9 months of vitamin E deprivation and after 14 months of vitamin E supplementation. Female normal mice did not develop mammary tumor during a comparable period of time. The mean catalase activity of mammary gland in acatalasemic mice was 18.8% of that in normal mice. The results indicate that vitamin E protects acatalasemic mice against the development of mammary tumor.

Keywords: Mammary tumorigenesis, Acatalasemic mouse, Vitamin E

Full Text

The Full Text of this article is available as a PDF (364.8 KB).

REFERENCE

  • 1.Warburg , O. , Gawehn , K. and Geissler , A. W.Ueber die Wirkung von Wasserstoffperoxyd auf Krebszellen und auf embryonale Zellen . Z. Naturforsch ., 12b , 393 – 395 ( 1957. ). [PubMed] [Google Scholar]
  • 2.Holman , R. A.A method of destroying a malignant rat tumor in vivo. Nature , 179 , 1033 ( 1957. ). [DOI] [PubMed] [Google Scholar]
  • 3.Heston , W. E. and Vlahakis , G.C3H‐Avy — a high hepatoma and high mammary tumor strain of mice . J. Natl. Cancer Inst ., 40 , 1161 – 1166 ( 1968. ). [PubMed] [Google Scholar]
  • 4.Andervont , H. B.Fate of the C3H mammary tumor agent in mice of strains C57BL, I, and BALB/c . J. Natl. Cancer Inst ., 32 , 1189 – 1198 ( 1964. ). [PubMed] [Google Scholar]
  • 5.Reddy , J. K. , Rao , M. S. and Moody , D. E.Hepatocellular carcinomas in acatalasemic mice treated with Nafenopin, a hypolipidemic peroxisome proliferator . Cancer Res ., 36 , 1211 – 1217 ( 1976. ). [PubMed] [Google Scholar]
  • 6.Watanabe , H. , Ogundigie , P. O. , Takahashi , T. , Ishimoto , T. and Ito , A.Induction of signet ring cell carcinomas in X‐irradiated hypocatalasemic mice (C3H/Cbs) . Jpn. J. Cancer Res ., 82 , 1175 – 1177 ( 1991. ). [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 7.Knekt , P. , Aromaa , A. , Maatela , J. , Aaran , R. K. , Nikkari , T. , Hakama , M. , Hakulinen , T. , Peto , R. and Teppo , L.Vitamin E and cancer prevention . Am. J. Clin. Nutr ., 53 , 283S – 286S ( 1991. ). [DOI] [PubMed] [Google Scholar]
  • 8.The α‐Tocopherol, β Carotene Cancer Prevention Study Group. The effect of vitamin E and beta carotene on the incidence of lung cancer and other cancers in male smokers . N. Engl. J. Med ., 330 , 1029 – 1035 ( 1994. ). [DOI] [PubMed] [Google Scholar]
  • 9.Buiatti , E. , Palli , D. , Decarli , A. , Amadori , D. , Avellini , C. , Bianchi , S. , Bonaguri , C. , Cipriani , F. , Cocc , P. , Giacosa , A. , Marubini , E. , Minacci , C. , Puntoni , R. , Russo , A. , Vindigni , C. , Fraumeni , J. F. and Blot , W. J.A case‐control study of gastric cancer and diet in Italy: II. Association with nutrients . Int. J. Cancer , 45 , 896 – 901 ( 1990. ). [DOI] [PubMed] [Google Scholar]
  • 10.Blot , W. J. , Li , J. Y. , Taylor , P. R. , Guo , W. , Dawsey , S. , Wang , G. Q. , Yang , C. S. , Zheng , S. F. , Gail , M. , Li , G. Y. , Yu , Y. , Liu , B. Q. , Tangrea , J. , Sun , Y. H. , Liu , F. , Fraumeni , J. F. , Zhang , Y. H. and Li , B.Nutrition intervention trials in Linxian, China: supplementation with specific vitamin/mineral combinations, cancer incidence, and disease‐specific mortality in the general population . J. Natl. Cancer Inst ., 85 , 1483 – 1492 ( 1993. ). [DOI] [PubMed] [Google Scholar]
  • 11.London , S. J. , Stein , E. A. , Henderson , I. C. , Stampfer , M. J. , Wood , W. C. , Remine , S. , Dmochowski , J. R. , Robert , N. J. and Willett , W. C.Carotenoids, retinol and vitamin E and risk of proliferative benign breast disease and breast cancer . Cancer Causes Control , 3 , 503 – 512 ( 1992. ). [DOI] [PubMed] [Google Scholar]
  • 12.Rohan , T. E. , Howe , G. R. , Friedenreich , C. M. and Miller , A. B.Dietary fiber, vitamins A, C, and E, and risk of breast cancer: a cohort study . Cancer Causes Control , 4 , 29 – 37 ( 1993. ). [DOI] [PubMed] [Google Scholar]
  • 13.Hunter , D. J. , Manson , J. E. , Colditz , G. A , Stampfer , M. J. , Rosner , B. , Hennekens , C. H. , Speizer , F. E. and Willett , W. C. , A prospective study of the intake of vitamins C, E, and A and the risk of breast cancer . N. Engl. J. Med ., 329 , 234 – 240 ( 1993. ). [DOI] [PubMed] [Google Scholar]
  • 14.Lee , C. and Chen , C.Enhancement of mammary tumorigenesis in rats by vitamin E deficiency . Proc. Am. Assoc. Cancer Res. Am. Soc. Clin. Oncol ., 20 , 132 ( 1979. ) ( AACR Abstracts ). [Google Scholar]
  • 15.Feinstein , R. N. , Braun , J. T. and Howard , J. B.Acatalasemic and hypocatalasemic mouse mutants . Arch. Biochem. Biophys ., 120 , 165 – 169 ( 1967. ). [DOI] [PubMed] [Google Scholar]
  • 16.The American Institute of Nutrition Ad Hoc Committee. Report of the American Institute of Nutrition Ad Hoc Committee on standards for nutritional studies . J. Nutr ., 107 , 1340 – 1348 ( 1977. ). [DOI] [PubMed] [Google Scholar]
  • 17.Johansson , L. H. and Borg , L. A. H.A spectrophotometric method for determination of catalase activity in small tissue samples . Anal. Biochem ., 174 , 331 – 336 ( 1988. ). [DOI] [PubMed] [Google Scholar]
  • 18.Feinstein , R. N.Perborate as substrate in a new assay of catalase . J. Biol. Chem ., 180 , 1197 – 1202 ( 1949. ). [PubMed] [Google Scholar]
  • 19.Smith , P. K. , Krohn , R. I. , Hermanson , G. T. , Mallia , A. K. , Gartner , F. H. , Povenzano , M. D. , Fujimoto , E. K. , Goeke , N. M. , Olson , B. J. and Klenk , D. C.Measurement of protein using bicinchoninic acid . Anal. Biochem ., 150 , 76 – 85 ( 1985. ). [DOI] [PubMed] [Google Scholar]
  • 20.van Assendelft , O. W. “ Spectrophotometry of Haemoglobin Derivatives ” ( 1970. ). Royal Vangorcum Ltd. , Assen , Netherlands . [Google Scholar]

Articles from Japanese Journal of Cancer Research : Gann are provided here courtesy of Wiley

RESOURCES