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. 2018 Jun 14;7:e35518. doi: 10.7554/eLife.35518

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Figure 3. — (A–L) Representative traces and summary bar graph for mEJPs and EJPs recorded at 0.8 mM Ca²⁺ from muscle 6 of indicated genotypes. The number of samples examined is indicated in each bar. (A–C) Neuronal knockdown of tnc significantly reduces the mEJPs frequency. (D–F) Neuronal but not muscle expression of Tnc can rescue the mEJPs frequency and EJP amplitude at tnc mutant NMJs. (G–L) The mean mEJPs frequency is dramatically reduced when mys/βPS integrin or if/αPS2 are knocked down in the neurons. Knockdown of if/αPS2 also induces slight reduction of the mean mEJPs amplitude and occasionally muscle attachment defects. (M–O) The trans-heterozygotes (mys/+;; tnc/+) show enhancement of phenotypes compared with individual heterozygotes, indicating that tnc and mys interact genetically. Bars indicate mean ±SEM. ns, not significant (p>0.05), ***p<0.001,***p<0.001 *p<0.05. Genotypes: N > tnc^RNAi (BG380-Gal4/+; UAS-tnc^RNAi/+); M > tnc^RNAi (UAS-tnc^RNAi/+; 24B-Gal4/+); tnc rescue control (UAS-tnc/+;tnc^EP/Df(3R)BSC655); N > tnc, tnc^EP/Df (UAS-tnc/+;tnc^EP/elav-Gal4,Df(3R)BSC655); M > tnc, tnc^EP/Df (UAS-tnc/+; tnc^EP/24B-Gal4, Df(3R)BSC655); N > mys^RNAi (BG380-Gal4/+; UAS-Dcr-2/+; UAS-mys^RNAi/+); M > mys^RNAi (UAS-Dcr-2/+; UAS-mys^RNAi/24B-Gal4); N > if ^RNAi (BG380-Gal4/+; UAS-if ^RNAi/UAS-Dcr-2); M > if ^RNAi (UAS-if ^RNAi/UAS-Dcr-2; 24B-Gal4/+).

Figure 3—figure supplement 1. — (A–L) Representative traces and summary bar graph for mEJPs and EJPs recorded at 0.8 mM Ca²⁺ from muscle 6 of indicated genotypes. The number of samples examined is indicated in each bar. (A–C) Neuronal knockdown of tnc significantly reduces the mEJPs frequency. (D–F) Neuronal but not muscle expression of Tnc can rescue the mEJPs frequency and EJP amplitude at tnc mutant NMJs. (G–L) The mean mEJPs frequency is dramatically reduced when mys/βPS integrin or if/αPS2 are knocked down in the neurons. Knockdown of if/αPS2 also induces slight reduction of the mean mEJPs amplitude and occasionally muscle attachment defects. (M–O) The trans-heterozygotes (mys/+;; tnc/+) show enhancement of phenotypes compared with individual heterozygotes, indicating that tnc and mys interact genetically. Bars indicate mean ±SEM. ns, not significant (p>0.05), ***p<0.001,***p<0.001 *p<0.05. Genotypes: N > tnc^RNAi (BG380-Gal4/+; UAS-tnc^RNAi/+); M > tnc^RNAi (UAS-tnc^RNAi/+; 24B-Gal4/+); tnc rescue control (UAS-tnc/+;tnc^EP/Df(3R)BSC655); N > tnc, tnc^EP/Df (UAS-tnc/+;tnc^EP/elav-Gal4,Df(3R)BSC655); M > tnc, tnc^EP/Df (UAS-tnc/+; tnc^EP/24B-Gal4, Df(3R)BSC655); N > mys^RNAi (BG380-Gal4/+; UAS-Dcr-2/+; UAS-mys^RNAi/+); M > mys^RNAi (UAS-Dcr-2/+; UAS-mys^RNAi/24B-Gal4); N > if ^RNAi (BG380-Gal4/+; UAS-if ^RNAi/UAS-Dcr-2); M > if ^RNAi (UAS-if ^RNAi/UAS-Dcr-2; 24B-Gal4/+).