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. 2018 Jun 14;7:e35518. doi: 10.7554/eLife.35518

Figure 9. Tnc directs accumulation of integrin and spectrin at the cell membrane.

(A–H) Phase contrast and confocal microscopy images of S2R + cells presented with control (Tld-HA-) and Tnc-HA-coated beads and stained for βPS, αPS2, α-Spectrin or Adducin (in red), HA (green), and DAPI (blue). Arrows indicate control and arrowheads point to Tnc-HA-coated beads, enlarged in details on the right. The Tnc-HA-coupled beads, but not the control, induce strong accumulation of βPS at the bead-cell membrane interfaces, as indicated in the lateral projections, along the dotted lines. The accumulation of αPS2, α-Spectrin or Adducin is also significant in the proximity of Tnc-HA-coupled beads, which appear yellow, in contrast to control beads that remain green. Scale bars: 5 μm, 1 μm in details.

Figure 9.

Figure 9—figure supplement 1. Cell expressing Tnc have elevated levels of βPS.

Figure 9—figure supplement 1.

(A–B) S2R + cells transfected with Tnc-HA stained for HA (green), βPS (red) and DAPI (blue) and analyzed by confocal microscopy. Comparing with control, cells expressing Tnc-HA have overall elevated βPS levels; the frequency and distribution of Tnc-HA and βPS-positive aggregates suggest that Tnc may aid the surface expression of βPS. Scale bars: 20 μm.