MicroRNA-183 inhibits A375 human melanoma cell migration and invasion by targeting Ezrin and MMP-9

Abstract

To assess the influence of microRNA-183 (miR-183) on the migration and invasion of A375 human melanoma cells, an A375 cell line with stable miR-183 overexpression or knockdown was constructed using lentiviral transfection. The change of miR-183 expression in these cells and in non-transfected controls was verified using reverse transcription-quantitative polymerase chain reaction. The impact of miR-183 on experimental A375 cell migration and invasion was assessed using a scratch and Transwell assay. The expression of Ezrin and matrix metalloprotease-9 (MMP-9), which are two mediator proteins that serve roles in tumor cell migration and invasion, were analyzed in each cell group via western blotting. The results of the present study indicated that miR-183 overexpression significantly inhibits A375 cell migration and invasion, which may be facilitated by miR-183 knockdown. Furthermore, Ezrin and MMP-9 protein levels were negatively associated with miR-183 expression, indicating that miR-183 may function as a tumor suppressor by inhibiting the expression of these two proteins. Additionally, miR-183 downregulation may be associated with the progression of melanoma.

Introduction

Melanoma is one of the fastest-growing malignancies worldwide (1,2), the incidence of which has continued to increase over the past several decades (2). Annually, this incidence increase varies among populations, ranging from 3 to 7%, which equates to a doubling of rates every 10 to 20 years (2). Although different treatment methods have been developed, metastasis remains a primary threat to patients with melanoma, resulting in high recurrence rates following therapy and a poor prognosis (3). Melanoma metastasis is a complex process, the mechanism of which is yet to be fully elucidated. However, several steps have been studied in the metastatic process of human melanoma, including dysregulated angiogenesis, angiotropism, cancer cell extravasation, immunoescape, epithelial-to-mesenchymal transition (EMT), capillary adhesion and colonization in specific organs (4,5). Cellular migration and invasion activity, which involve the dysregulation of multiple key adapters or signaling pathways, are considered vital determinants of melanoma metastasis and as such are attracting wide research interests (4,6).

In recent years, the dysregulation of Ezrin and matrix metalloprotease-9 (MMP-9) has been determined to be an important metastasis-associated mechanism in tumor cells of different tissue origins (7–12). Ezrin, a 69 kDa cytoplasmic peripheral membrane protein belonging to the Ezrin/radixin/moesin (ERM) protein family, is an essential factor for gynecologic cancer (13), osteosarcoma (14), rectal cancer (15,16) and melanoma (17) metastasis. Together with other ERM family proteins, Ezrin serves as an intermediate between cell membrane proteins and the actin cytoskeleton (18), regulating a series of cellular activities including cell survival, adhesion, migration and invasion (18,19). It has been confirmed in multiple different tumor models that Ezrin overexpression may contribute to tumor metastasis and invasion (20–24), potentially by inducing and promoting the EMT process (22,25–27). MMP-9 is a 92 kD matrixin (a type of enzyme), belonging to the zinc-metalloproteinase family, which functions to degrade the extracellular matrix (28). Mostly secreted as inactive pro-proteins and activated following cleavage by other extracellular proteinases, MMPs serve a role in extracellular matrix breakdown in certain physiological processes, including embryonic development, angiogenesis, wound healing and cell migration, as well as in some pathological progressions, including cancer cell invasion and metastasis (29). Emerging evidence has indicated that the overexpression or hyper-activation of MMP-9 contributes to tumor metastasis by facilitating tumor cell migration and invasion in different models (10,30,31).

Recently, abnormal changes in microRNA (miRNA or miR) levels have been proposed to be a primary mechanism for the dysregulation of effector proteins associated with tumor development (32–36). MiRNAs are small non-coding RNAs that serve a function in gene silencing via sequence-specific base pairing with the 3′ or 5′ untranslated regions of target mRNAs (37). This results in mRNA destabilization and degradation, thus serving as an important post-transcriptional regulatory mechanism (37). The dysregulation or loss of function of certain key regulatory miRNAs may therefore contribute to tumor development via the subsequential dysregulation of tumor suppressor proteins or oncoproteins (38). Several studies have indicated that a change in human miR-183 level is closely associated with tumor development (39–44). However, the influence of miR-183 expression change on melanoma is yet to be fully elucidated.

Previous studies have demonstrated that Ezrin and MMP-9 are direct targets of miR-183 regulation, such that a decrease of miR-183 causes the upregulation of Ezrin and MMP-9, which leads to the facilitated migration and invasion of tumor cells with different tissue origins (45–51). To assess the impact of miR-183 dysregulation in melanoma, the present study constructed an A375 human melanoma cell line with stable miR-183 overexpression or knockdown via lentiviral transfection with an miR-183 mimic or inhibitor, respectively. Changes in miR-183 expression in the miR-183 mimic, inhibitor and control group was verified using a reverse transcription-quantitative polymerase chain reaction (RT-qPCR) assay following total RNA isolation. Furthermore, the impact of miR-183 on the migration and invasion of A375 cells was evaluated using a scratch and Transwell assay, respectively. Ezrin and MMP-9 levels were analyzed using western blotting in each group. The results indicated that miR-183 functions as a tumor suppressor in melanoma by inhibiting tumor cell migration and invasion via the downregulation of Ezrin and MMP-9 protein and the deregulation of miR-183. Therefore, miR-183 may be an important factor for melanoma metastasis and development. These results may provide novel insights into the molecular mechanism of melanoma progression and may also elucidate a target for the development of novel gene therapy against melanoma and other metastatic tumors.

Materials and methods

Cell culture

A375 cells (Chinese Academy of Sciences Cell Bank, Beijing, China) were cultured in RPMI-1640 medium (Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA) supplemented with 10% fetal bovine serum (Biological Industries, Kibbutz Beit Haemek, Israel) and maintained at 37°C in a humidified incubator with 5% CO₂, unless otherwise indicated.

MiR-183 overexpression and knockdown in A375 cells and the evaluation of miR-183 mRNA expression

The sequence of miR-183 is 5′-GUGAAUUACCGAAGGGCCAUAA-3′. MiR-183 overexpression and knockdown in A375 human melanoma cells was achieved using an miR-183 mimic, an miRNA mimic negative control (NC) [an insignificant short nucleotide sequence], an miR-183 inhibitor, an miRNA inhibitor NC and blank plasmids were all obtained from Guangzhou RiboBio Co., Ltd. (Guangzhou, China) following the manufacturer's protocol. Following transfection miR-183 (250 µg/ml) for 12 h by Lipofectamine 2000 (Thermo Fisher Scientific, Inc.) according to the manufacturer's protocol, total RNA from each cell group was isolated via TRIzol-chloroform-isopropanol (TaKaRa, Bio, Inc., Otsu, Japan) extraction and miR-183 mRNA expression was assessed using RT-qPCR analysis with an All-in-One™ miRNA RT-qPCR detection kit (GeneCopoeia, Inc., Rockville, MD, USA) following the manufacturer's protocol. The sequences of miR-183 primers, forward: 5′-CGCGGTATGGCACTGGTAGA-3′, and reverse: 5′-AGTGCAGGGTCCGAGGTATTC-3′. MiR-183 expression levels were normalized to levels of U6 snRNA (5′-AAATTCGTGAAGCGTTCC-3′) using the 2^−ΔΔCq method (52). Detection was achieved by SYBR green qPCR with the following conditions: 95°C for 10 min followed by 40 cycles of 95°C for 10 sec, 60°C for 20 sec and 72°C for 20 sec.

Scratch assay

A375 cells transfected with the miR-183 mimic, mimic NC, miR-183 inhibitor or inhibitor NC plasmid were cultured (37°C) overnight into confluent monolayers. Scratches were generated gently in each cell culture using a sterile 200 µl pipette tip. Cell migration towards the mid-line of the scratch was monitored at 0 and 24 h using a light microscope at ×100 magnification.

Transwell migration and invasion assays

Following pre-culture in serum-free RPMI-1640 medium for 24 h at 37°C, A375 cells with the aforementioned transfections were trypsinized, re-suspended in serum-free RPMI-1640 medium and diluted to a density of ~10,600 cells/ml. Cells (100 µl of each group) were then seeded in the inner chamber membrane of the Transwell apparatus. For the assessment of invasion activity, the inner chamber was coated with fibronectin under serum-free conditions and the uncoated inner chamber was used for the evaluation of migration activity. RPMI-1640 medium supplemented with 50% fetal bovine serum was added in the lower chamber of the Transwell apparatus in each group and cells were cultured for 24 h at 37°C in a humidified incubator with 5% CO₂. A549 cells on the lower surface of the inner chamber were then gently rinsed with PBS and stained with crystal violet staining solution at room temperature for 10 min (Beyotime Institute of Biotechnology, Haimen, China) prior to being counted using light microscopy at ×200 magnification. Five randomly selected fields of view were selected.

Western blotting

A375 cells that underwent the aforementioned transfections were cultured in 6-well plates under normal culture conditions (37°C for 24 h). A375 cells were homogenized in lysis buffer [10 mM Tris base, pH 7.4, 150 mM NaCl, 1 mM EDTA, 1 mM EGTA, 1% Triton X-100, 0.5% NP-40, and a protease and phosphatase inhibitor cocktail (Thermo Fisher Scientific, Inc.)]. Protein concentrations were determined using a BCA assay kit (Thermo Fisher Scientific, Inc.) with bovine serum albumin (2 mg/ml) as a standard. The total protein of each cell group (20 µl) was separated using polyacrylamide gel (15%) electrophoresis under reducing conditions, followed by transferral onto nitrocellulose membranes. Following blocking at 4°C overnight with non-fat milk and preparation with PBS, membranes were probed with antibodies (incubated 16 h at 4°C) targeting human Ezrin (cat. no. ab40839; Abcam, Cambridge, UK; dilution of 1:2,000) and MMP-9 (cat. no. ab76003; Abcam; dilution of 1:1,000), with β-actin (cat. no. ab8227; Abcam; dilution of 1:2,000) as an internal reference. The membranes were then incubated with horseradish peroxidase conjugated secondary antibodies (dilution of 1:5,000, incubated 1 h at room temperature) (Goat Anti-Rabbit IgG H&L; cat. no. ab6702; Abcam) and colorized using an ECL substrate (Thermo Fisher Scientific, Inc.). The image of each sample was captured using chemo-fluorescence-sensitive film and the gray value of each band was analyzed. The quantity of target protein was evaluated by the ratio of its gray value to the internal reference using ImageJ software, version 1.48 (National Institutes of Health, Bethesda, MD, USA).

Statistical analysis

All experiments were independently repeated at least three times. Statistical analysis was performed using SPSS statistical software, version 17.0 (SPSS, Inc., Chicago, IL, USA). All statistical data were presented as the mean ± standard deviation. A Student's t-test was performed to assess significance and P<0.05 was considered to indicate a statistically significant difference.

Results

Analysis of miR-183 expression following transfection

To determine the influence of increased and decreased miR-183 expression in human melanoma, A375 cells were transfected with miR-183 mimic and miR-183 inhibitor sequences, which served as the experimental groups. MiRNA mimic and inhibitor NC sequences were utilized as internal reference groups, using a lentiviral plasmid transfection system. A375 cells that were non-transfected and transfected with blank plasmids were used as external references. MiR-183 expression in each group 12 h following transfection was analyzed using RT-qPCR following total RNA isolation. The results demonstrated that the expression of miR-183 in the miR-183 mimic group was >16-fold higher compared with all control groups, while the miR-183 inhibitor group was 5-fold lower compared with control groups (Fig. 1). These results indicated that the established cell line was stable. MiR-183 levels in the mimic and inhibitor NC groups were similar to those of the blank and NC groups, indicating that transfection alone did not affect miR-183 expression. Additionally, no notable morphological changes were observed among A375 cells in the blank, NC, mimic NC and inhibitor NC groups (data not shown). Therefore, A375 cells in mimic NC and inhibitor NC groups alone were utilized as NCs in future experiments.

Figure 1.

Comparison of miR-183 expression in A375 human melanoma cells with different transfections. The blank group consisted of non-transfected A375 cells. A375 cells transfected with blank plasmids were utilized as the NC. Data are presented as fold change relative to the mean expression of the NC group. ***P<0.001. miR, microRNA; NC, negative controls.

MiR-183 inhibits the migration of A375 cells in vitro

To assess the influence of miR-183 expression changes on A375 cell migration, a scratch assay was performed as aforementioned. The results demonstrated that the artificial upregulation of miR-183 expression significantly suppressed A375 cell migration, whereas miR-183 downregulation promoted A375 cell migration when compared with their respective controls (Fig. 2). This indicated that miR-183 inhibits the migration of A375 cells in vitro. To further evaluate the influence of miR-183 expression change on A375 cell migration and invasion, a Transwell assay was performed with each group of cells as aforementioned. The results demonstrated that miR-183 overexpression in the miR-183 mimic group significantly inhibited the migration and invasion of A375 cells in vitro compared with the mimic NC group (Fig. 3A and B). Furthermore, miR-183 knockdown in the inhibitor group promoted A375 cell migration and invasion compared with the inhibitor NC group (Fig. 3C and D). Taken together, this indicates that miR-183 suppresses A375 cell migration and invasion in vitro, and migration and invasion are increased by the downregulation of miR-183.

Figure 2.

Scratch assay to compare human melanoma A375 cell migration with different transfections. Representative results of A375 cells transfected with the (A) miRNA inhibitor NC (Inhibitor NC) and the (B) miR-183 inhibitor. Representative results of A375 cells transfected with the (C) miRNA mimic NC and the (D) miR-183 mimic. All images were obtained using a light microscope at 0 or 24 h following the scratch assay. The magnification of the images was ×100. (E) Statistical analysis of the scratch assay results at 24 h. The mobility ratio of each experiment was measured by dividing the scratch width at 24 h by that at 0 h. **P<0.01; ****P<0.0001. miRNA and miR, microRNA; NC, negative controls.

Figure 3.

Transwell assay to compare human melanoma A375 cell migration and invasion with different transfections. (A and C) Representative results of the Transwell assay. Cells on the lower surface of the inner chamber membrane were stained with crystal violet prior to gentle rinsing with PBS. (B and D) Statistical analysis of experimental group cell counts. A total of five microscopic fields of view (at ×200 magnification) were randomly selected from each inner membrane and cells were counted. *P<0.05; **P<0.01; ***P<0.001. NC, negative control; miR, microRNA.

Western blot analysis of Ezrin and MMP-9 in miR-183 overexpression or knockdown A375 cells

To determine the inhibitory mechanism of miR-183, changes in Ezrin and MMP-9 protein expression in A375 cells were assessed at 12 h following transfection with an miR-183 mimic or inhibitor. Western blotting revealed that Ezrin and MMP-9 were downregulated and upregulated following miR-183 overexpression and knockdown in vitro, respectively (Fig. 4). Considering the well-established association of Ezrin and MMP-9 with the migratory and invasive activity of melanoma, and that these proteins act as direct targets of miR-183 (45,46,49,53), the results demonstrated that miR-183 inhibits A375 cell migration and invasion potentially via the downregulation of Ezrin and MMP-9 expression.

Figure 4.

Comparison of Ezrin and MMP-9 protein expression in A375 human melanoma cells with different transfections. Representative western blotting results of (A) Ezrin and (B) MMP-9 protein expression 12 h following transfection in each experimental group. β-actin was utilized as an internal reference. Statistical analysis of (C) Ezrin and (D) MMP-9 protein expression in each experimental group. Data were acquired by measuring the gray value of each bar from the results of western blotting and were presented as the fold change relative to that of β-actin. *P<0.05. MMP-9, matrix metalloprotease-9; NC, negative controls; miR, microRNA.

Discussion

The results of the present study support the theory that miR-183 may serve as a tumor suppressor in melanoma. The migratory activity and invasiveness of A375 human melanoma cells was demonstrated to be negatively associated with miR-183 expression. Furthermore, Ezrin and MMP-9, which have been previously regarded as important promoters of melanoma metastasis (54–59), were revealed to be negatively regulated by miR-183, indicating that miR-183 inhibits A375 human melanoma cell migration and invasion, possibly through the downregulation of Ezrin and MMP-9 expression. Thus, the abnormal decrease of miR-183 in melanoma may cause Ezrin and MMP-9 overexpression, resulting in an increased metastatic activity. Several studies have revealed that miR-183 may exhibit either pro- or anti-metastatic functions in different tumor cells (60–65). More specifically, miR-183 has been considered to promote metastasis in most tumor models, including breast cancer (66), medulloblastoma (67), hepatocellular carcinoma (68), follicular thyroid carcinoma (43), esophageal cancer (69), gastric cancer (70), pancreatic cancer (71) and synovial sarcoma (72), while inhibiting metastasis in lung (73), colon (74) and ovarian cancer (75). This is possibly due to the heterogeneity of cancer and the differences in ontogenesis of different types of cancer.

MiR-183 is located and transcribed in cluster with miR-96 and miR-182, which exerts various functions (40). It regulates multiple mRNA targets; however, not all targets have been fully elucidated (38). Further studies are thus required to assess the transcriptional regulation of the miR-183-96-182 cluster under tumor pathological conditions. Considering the complexity of cytokinetic regulation, proteomic methods that assess the function and regulatory network of miR-183 target proteins, and particularly their interaction with cytoskeleton regulators and integrins, which interact with the extracellular matrix, are being employed (42). However, further studies are required to obtain a better understanding of the regulatory mechanism of miR-183. This may provide novel insights into the development of anti-metastasis gene therapy.

Funding

No funding was received.

Availability of data and materials

All data generated or analyzed during this study are included in this published article.

Authors' contributions

YSZ and GQW completed all the experiments and the statistical analysis. YSZ wrote and revised the manuscript. All authors read and approved the final the manuscript.

Ethics approval and consent to participate

Not applicable.

Patient consent for publication

Not applicable.

Competing interests

The authors declare that they have no competing interests.