Skip to main content
Cancers logoLink to Cancers
. 2019 Mar 1;11(3):292. doi: 10.3390/cancers11030292

Role of Notch Signaling Pathway in Glioblastoma Pathogenesis

Riccardo Bazzoni 1,2,3, Angela Bentivegna 3,4,*
PMCID: PMC6468848  PMID: 30832246

Abstract

Notch signaling is an evolutionarily conserved pathway that regulates important biological processes, such as cell proliferation, apoptosis, migration, self-renewal, and differentiation. In mammals, Notch signaling is composed of four receptors (Notch1–4) and five ligands (Dll1-3–4, Jagged1–2) that mainly contribute to the development and maintenance of the central nervous system (CNS). Neural stem cells (NSCs) are the starting point for neurogenesis and other neurological functions, representing an essential aspect for the homeostasis of the CNS. Therefore, genetic and functional alterations to NSCs can lead to the development of brain tumors, including glioblastoma. Glioblastoma remains an incurable disease, and the reason for the failure of current therapies and tumor relapse is the presence of a small subpopulation of tumor cells known as glioma stem cells (GSCs), characterized by their stem cell-like properties and aggressive phenotype. Growing evidence reveals that Notch signaling is highly active in GSCs, where it suppresses differentiation and maintains stem-like properties, contributing to Glioblastoma tumorigenesis and conventional-treatment resistance. In this review, we try to give a comprehensive view of the contribution of Notch signaling to Glioblastoma and its possible implication as a target for new therapeutic approaches.

Keywords: glioblastoma, GSCs, Notch signaling pathway, new therapeutic approaches

1. Introduction

Glioblastoma is the most common and fatal type of primary brain tumor [1]. It comprises 70% of all gliomas and is classified as a Grade IV astrocytoma (World Health Organization classification) [2]. The current standard of care for Glioblastoma patients includes maximal safe resection, followed by concurrent radiotherapy (RT) and chemotherapy with temozolomide (TMZ), followed by adjuvant TMZ [3]. Despite these aggressive therapies, the median survival time is currently 14.6 months [4], with a final mortality rate of close to 100% [5]. The failure of current therapies is due to the coexistence of heterogeneous tumor cell populations with different grades of differentiation [6,7] and the presence of a small subset of tumor cells that display stem cell-like properties, which are responsible for tumor relapse after conventional treatments [8,9]. This population, known as glioma stem cells (GSCs), exhibits an enhanced self-renewal capacity, compromised differentiation, and in vivo tumorigenicity, besides being radio- and chemoresistant [10,11]. Recent studies have demonstrated that Notch signaling is highly active in GSCs, contributing to suppressing differentiation and maintaining stem cell-like properties. Therefore, this review aims to discuss the role of Notch signaling in Glioblastoma pathogenesis and in the development of potential therapeutic strategies.

2. Notch Signaling Pathway Overview

Notch signaling is an evolutionarily conserved pathway that plays a critical role in various cellular and developmental processes, including cell proliferation, apoptosis, stem cell maintenance, cell fate decision, and tissue homeostasis [12,13,14]. Notch functions as a cytoplasmic receptor and, in mammals, there are four homologous proteins known as Notch1, Notch2, Notch3, and Notch4, which can bind two ligands families: Delta-like (Dll1-3 and -4) and Jagged (Jagged1 and -2) [15]. Both the receptors and ligands are single-pass transmembrane proteins (Figure 1). The interaction between Notch and its ligands can occur in two ways: In trans, when they are present on neighboring cells, or in cis, when the receptor and ligand are present on the same cell [16,17]. In the first case, binding leads to pathway activation, while in cis form interaction inhibits the signaling cascade [18].

Figure 1.

Figure 1

Structure of human Notch receptors and ligands.

Structurally, the Delta and Jagged ligands are very similar: They lack an intracellular domain, while their extracellular portion is constituted by 6–16 EGF-like repeats [19] and a distal Cys-rich region called the Delta/Serrate/Lag-2 (DSL) domain [20]. The DSL region is responsible for interaction with Notch receptors [21]. Moreover, Jagged1 and -2 possess a Cys-rich region closer to the membrane [21]. Unlike Delta and Jagged, the extracellular domain of Notch receptors is organized in 29–36 EGF-like repeats [19,21] and exists in a Cys-rich region called Lin-12 that noncovalently binds the extracellular Notch with the membrane-tethered intracellular Notch [18]. The Notch intracellular domain (NICD) is composed of an RBPJK associate molecule (RAM) region, repeated structural motifs named ankyrin repeats (which mediate the interaction between NICD and CBF1, a transcriptional activator), a transactivation domain (TAD), and a PEST domain (involved in Notch degradation) [21].

The life of Notch receptors begins in the endoplasmic reticulum (ER), where they are synthesized as inactive single-peptide precursors (Figure 2).

Figure 2.

Figure 2

Schematic representation of the Notch signaling pathway. Once synthesized in the endoplasmic reticulum (①), the inactive single peptide precursor moves to the Golgi where it is cleaved by a furin-like convertase (S1 cleavage) (②) and translocates into the cell membrane. The binding with a Notch ligand (③) induces the second cleavage (S2) by a member of the disintegrin and metalloproteinases (ADAM) family (④), resulting in the formation of a membrane-tethered Notch truncated (NEXT) fragment, which is further processed in two sites (S3 and S4) by a presenilin-dependent γ-secretase complex (⑤), generating the Notch intracellular domain (NICD), the active form of the Notch receptor (⑥). The NICD can now enter into the nucleus, where it exerts its transcriptional activity (⑦). The ubiquitination of the NICD (⑧) leads to its proteasome degradation (⑨).

Once in the trans-Golgi, a furin-like convertase proteolytically cleaves the inactive precursor [22]. This first cleavage site (S1) allows the mature Notch receptor to form, which is able to translocate in the cytoplasmic membrane and interact with its ligands [22]. The in-trans activation triggers a conformational change in the Notch receptor that exposes the second cleavage site (S2) to a member of the disintegrin and metalloproteinases (ADAM) family (ADAM10 or ADAM17) [23,24]. The result is a membrane-tethered Notch-truncated (NEXT) fragment [24], which is further processed into two sites (S3 and S4) by a presenilin-dependent γ-secretase complex, constituted by presenilin 1 (PSEN1) or PSEN2, nicastrin, presenilin enhancer 2 (PEN2), and anterior pharynx-defective 1 (APH1) [25,26]. These cleavages release the active form of the NICD into the cytoplasm. The NICD can then translocate into the nucleus [27], where it binds several DNA-binding proteins of the CSL family (RBPJK/CBF1/KBPF2 in mammals). The newly formed complex switches from a transcriptional-repressor state to a transcriptional-activator one, recruiting the transcriptional coactivator mastermind-like protein (MAML), the Ski-interacting Protein (SKIP), and histone acetyltransferases CBP/p300 and PCAF/GCN5 [28,29,30]. All these events allow the transcription of transcriptional repressors such as the Hairy Enhancer of Split (Hes) family of proteins and HES-related proteins (Hey) [31,32], two families responsible for lineage-commitment decisions. Other Notch target genes include p21/Waf1, cyclin D1 and -3, c-Myc, HER2, Notch-regulated ankyrin repeat protein (NRAR), NF-κB, pre-Tα, IGF1-R, survivin, Snail homolog 2 (SLUG), SOX2, and PAX5 [33,34,35,36]. The Notch-mediated transcriptional activation ends with the degradation of the NICD. The mechanism consists of the phosphorylation of a degron within the PEST domain of NICD, mediated by cyclin-dependent kinase 8 (CDK8) and targeted for proteasome-mediated degradation by E3 ubiquitin ligases SEL10 (also known as FBW7) [18,37].

In addition to canonical signaling activation, several groups have identified a novel way to activate the Notch pathway through a group of different, unrelated proteins that lack the DSL domain [38,39]. These proteins can be membrane-integral (for example, the Delta/Notch-like epidermal growth factor-related receptor—DNER), a glycosylphosphatidylinositol (GPI)-linked membrane (for example NB3/Contactin6), or secreted proteins (for example, MAGP1 and -2) [40]. Notably, Notch can non-canonically exert its biological functions either in a ligand-dependent or -independent way [41].

3. Notch Signaling in Brain Development

The Notch signaling pathway plays a fundamental role in central nervous system (CNS) development, from embryonic stages to the adult brain [41]. Although Notch signaling members show differential expression patterns throughout the brain (Table 1), they are master regulators of neurogenic niches—specialized microenvironments that are able to modulate the properties of stem cells, such as their cell number, self-renewal, and fate decision, in order to avoid the depletion of the NSC pool [42,43]. Being the starting point for neurogenesis, NSCs are extremely important [44]. In the adult brain, there are two major sites for neurogenesis: The subventricular zone (SVZ) of the lateral ventricle [43,45] and the dentate gyrus (DG) in the hippocampus [46].

Table 1.

Expression pattern of Notch receptors and ligands in the adult brain.

Notch Signaling Members Expression Pattern References
Notch1 Neurons, astrocytes, precursor cells, ependymal cells, endothelium [47,48,49,50]
Notch2 Precursor cells [47,51]
Notch3 Precursor cells [51]
Notch4 Endothelium [52]
Delta/Notch-like epidermal growth factor-related receptor (DNER) Neurons [53]
Dll1 Intermediate neural progenitors, neurons [49,51,54,55]
Dll3 Intermediate neural progenitors [54]
Dll4 Endothelium [56]
Jagged1 Precursor cells, intermediate neural progenitors, neurons [48,49,54,57,58,59]
Jagged2 Neurons [49,52]

It was demonstrated that low Notch levels induce the proliferation of NSCs, followed by an exit from the cell cycle and differentiation into neurons [60]. On the contrary, high Notch levels lead to growth arrest, or even induce a quiescent state [61,62]. The cell-fate regulation of quiescent NSCs results under RBPJK activity, probably through Notch2 and Notch3, but not Notch1 [63,64]. Instead, Notch1 seems to be crucial for the active proliferation of the NSC pool, which is selectively lost during aging [43,46]. Additionally, the ablation or loss of RBPJK function shows progenitor-cell depletion in the postnatal SVZ and DG, and reduced neurogenesis [46,65]. Thus, it is plausible to conclude that Notch signaling is instrumental for the regenerative capacity of a mature brain. Concerning neuronal migration, in the cerebral cortex, reelin-DAB1 signaling avoids NICD degradation, promoting Notch signaling in order to alter neuron morphology and, therefore, perturbing their migration [66]. This interaction between reelin-DAB1 and NICD was also reported in a study by Sibbe et al., where they showed that this mechanism is essential for the correct development of the radial glial scaffold [67].

To explicate their functions, neurons require glial cells which surround and insulate them, providing physical support. Like neurons, glial cells differentiate from NSCs [68]. During neurogenesis, Notch receptors are ubiquitously expressed, whereas Delta ligands appear transiently on the surface of newly differentiating neurons. NSCs, when exposed to a Delta signal, tend to resist prevailing neurogenic signals, activate Notch signaling and ultimately differentiate into glial cells. Therefore, the resistance to neurogenic signals is dependent on the activation of Notch by Delta [69,70]. Recently, several studies have suggested a new model in which Notch signaling prevents all types of NSC differentiation, rather than inhibiting neural differentiation. According to this view, NSCs will choose their fate according to whichever instructive factors prevail when they are released from the influence of Delta [71]. Indeed, Notch signaling seems to have an instructive role in gliogenesis, promoting the differentiation of many glial subtypes, with the exception of oligodendrocytes. The results of in vivo studies are consistent with the notion that Notch signaling plays an instructive role in gliogenesis through their conventional basic helix-loop-helix (bHLH) targets [72,73]. Moreover, if Notch activation promotes glial differentiation, it would be expected that some Notch receptors downstream elements (for example transcriptional targets [74]) would also influence gliogenesis.

Finally, although the role of Notch signaling in brain function is complicated and controversial, all this evidence points out the essential role of Notch in several aspects of the CNS.

4. Notch Pathway Deregulation in Brain Tumors and Brain CSCs

Deregulation of the Notch pathway has been associated with the development of a wide range of diseases through both germline and somatic mutations. Concerning the latter events, Notch signaling mutations lead to cancer and malignancy, of which the best-known example is T-cell acute lymphoblastic leukemia [75,76]. Over the years, aberrant Notch signaling was also found in several solid tumors, including brain tumors, although its components are rarely mutated [76,77].

Brain tumors are a heterogeneous group of neoplasms. Among them, the most common are gliomas and medulloblastomas [2]. Several studies have reported the abnormal expression of various Notch components in brain tumors. For instance, a higher expression of ASCL1, Dll1, Notch1, -3, -4, and Hey1 correlates with a higher glioma grade and a worse prognosis [78,79,80,81], indicating that more activated Notch signaling promotes a more undifferentiated and aggressive tumor phenotype.

In the last twenty years, the cancer stem cell (CSC) theory has said that CSCs are responsible for tumor development and growth, as well as being the reason for cancer metastasis and relapse [33,82,83,84,85]. Brain CSCs (bCSCs), or brain-tumor initiating cells (bTICs), share similarities with NSCs [86]: They are able to grow as floating aggregates (called neurospheres) in a serum-free medium [87,88], express stem cell markers (such as nestin, GFAP, β-III tubulin, and CD133) [87,89], and can differentiate into all three neural lineages [87,89,90]. Since Notch signaling is a key regulator of the NSC state, it is easy to suppose its involvement in the maintenance of the NSC tumor counterpart. For instance, intracellular modulators such as Numb4 and Numb4Δ7 regulate the expression of stem cell markers in bCSCs, despite functioning like an inhibitor and activator for Notch signaling, respectively. This assumes that Notch mediators can alter bCSC differentiation independently from Notch inhibition itself [91]. Furthermore, NICD-overexpressing bCSCs induce tumor formation in nude mice [92].

bCSCs are generally more resistant to treatment under hypoxic conditions. In support of this, the depletion of HIF-1α alters the proliferation of glioma-derived bCSCs through blocking the interaction of HIF-1α and NICD [93]. This interaction was confirmed by another group, which showed that HIF-1α displaced HIF-2α (a Notch inhibitor) from the NICD under hypoxic conditions [94]. From these data, it emerges that active Notch signaling is necessary for maintaining stem cell features and the tumorigenic potential of bCSCs, and sets the stage for promising Notch-based therapies.

5. Notch Signaling and Glioblastoma

5.1. Expression Pattern of Notch Signaling in Glioblastoma

mRNA and protein levels of Notch1, Notch4, Dll1, Dll4, Jagged1, CBF1, Hey1, Hey2, and Hes1 are higher in brain tumor cells than normal brain cells, correlating with an elevated expression of VEGF and pAKT, and reduced levels of PTEN [79,95,96,97,98]. In particular, Notch1 expression is higher in the survival of >1 year patients than <1 year [99], whereas Notch1 overexpression is associated with low overall survival (OS) [100], suggesting a controversial role of Notch1 in gliomagenesis. Moreover, Notch1 is more expressed in peritumor-tissue GSCs compared to tumor-core GSCs [101]. Notch1 and Notch4 levels correlate with those of GFAP and vimentin, respectively. Notch4 expression increases with higher-grade and primary tumors [102]. Notch2 expression levels in Glioblastoma tissue correlate with stemness genes (nestin, SOX2), astrocyte fate genes (vimentin and GFAP), and anti-apoptotic proteins (BCL6 and BCL-W), but are inversely correlated with Olig2, CNP, and PLP1 (oligodendrocyte fate) and pro-apoptotic proteins (BAX and BCLAF1) [102,103]. The overexpression of Hey1, which is associated with survival and tumor grade, might be due to the impairment of Notch and E2F signaling; it was demonstrated that its overexpression in NSCs triggers neurosphere formation and contributes to Glioblastoma proliferation [95]. On the contrary, several groups reported a weak expression of Notch1, Notch2, MAML1, and p300 in Glioblastoma [102,104,105]. Intriguingly, the impairment of Notch signaling in secondary Glioblastoma, in which Hes1 expression is almost absent, is associated with the overexpression of ASCL1. On the other hand, the activation of Notch signaling in primary Glioblastoma is associated with low levels of ASCL1, suggesting that Notch inhibition via ASCL1 upregulation might be responsible for a potential progression into secondary Glioblastomas [78].

Correlation between Glioblastoma molecular subtypes and Notch expression was also demonstrated. Concerning the mesenchymal subtype (the most aggressive one), Notch-related genes are the most highly enriched in high p-STAT3 patients, suggesting a synergy between Notch and STAT3 signaling [106]. Verhaak et al. reported that Notch signaling is highly expressed in the classic subtype [107]. The expression levels of Dll3 and Hey2 are low in proneural Glioblastomas, while the expression level of Notch1 is high [81,105,107,108], although Cooper et al. reported reduced levels of Dll3 and Hey2 [108]. Always concerning the proneural subtype, the majority of Glioblastomas with the IDH mutation have a proneural gene expression pattern, even if only 30% of proneural Glioblastomas have the mutation [109]. Spino et al. reported that IDH-mutant gliomas (mostly low grade) have high and homogenous Dll3 expression, whereas approximately half of IDH-wild type Glioblastomas had either no expression or only scattered cells expressing Dll3. Regardless, Dll3 expression, if present in a IDH-wild type Glioblastoma, is generally restricted to non-mesenchymal subtypes [110]. Notably, Jungk et al. provided a link between Notch expression and tumor location in the proneural subtype. They observed that, in IDH-wild-type tissue near to the SVZ, the overexpression of Hes4 and Dll3 predicts inferior OS [111].

Finally, even the non-canonical Notch pathway plays a role in gliomagenesis. Huber et al. found that Deltex1 (DTX1) levels were higher in Glioblastoma compared to the normal brain, inducing several pathways involved in glioma aggressiveness such as RTK/PI3K/PKB and MAPK/ERK signaling, and the anti-apoptotic protein Mcl-1 [112].

5.2. Epigenetic Regulation of Notch Signaling in Glioblastoma

A peculiar characteristic of epigenetic alterations is their reversibility, making them a promising therapeutic target to explore in order to reset the abnormal cancer epigenome. To date, we do not know much about the epigenetic regulation of Notch signaling in Glioblastoma. Tsung et al. showed how the methylation status of the transcription factor HEY1 contributes to Glioblastoma pathogenesis [113]. They found low levels of methylation on CpG islands within the HEY1 promoter across Glioblastoma specimens when compared to a healthy brain, resulting in Hey1 overexpression [113]. In support of this, treatment with sodium butyrate (NaB), a histone deacetylase (HDAC) inhibitor, on 4910 and 5310 xenograft cell lines induced Glioblastoma cell apoptosis, decreased Hey1 expression, and increased DNMT1 levels. Moreover, the knockdown of HEY1 reduced cell invasion, migration, and proliferation [113]. Sun et al. highlighted the role of the Delta/Notch-like epidermal growth factor-related receptor (DNER), which regulates cerebellar development and neurodevelopmental interactions between Purkinje cells and Bergmann glia which express Notch via a Deltex-dependent mechanism [114]. HDAC inhibition is able to activate the DNER/Deltex signaling pathway in Glioblastoma-derived neurospheres, resulting in cell differentiation and neurosphere-growth inhibition [114]. However, due to lack of sufficient evidence relating to the epigenetic regulation of the Notch signaling pathway in Glioblastoma, to date there are no epigenetic Notch biomarkers for cancer diagnosis.

5.3. Role of miRNAs in Notch-Dependent Gliomagenesis

MicroRNAs (miRNAs or miRs) are small (20–22 nucleotides), non-coding RNA molecules that can play a gene-regulatory role by pairing to the mRNAs of protein-coding genes to direct the inhibition of their translation or induce their destabilization and degradation. By regulating gene expression and therefore various cell processes, like proliferation and apoptosis, their alterations are often associated with the pathogenesis of several cancers. Starting from a network topological analysis of the Glioblastoma Notch regulatory network, Sun et al. pointed out 32 miRNAs that might be involved in the Notch pathway, and six of them (miR-9, miR-34a, miR-92b, miR-124, miR-137, and miR-219-5p) might play a key role [115]. Among the Notch-related miRNAs involved in gliomagenesis (Figure 3). The miR-34 family is the most studied. It is downregulated in Glioblastoma tissue compared to normal brain tissue and is more expressed in wild-type p53 Glioblastomas than mutant p53 Glioblastomas [116,117].

Figure 3.

Figure 3

Functional effects of Notch-regulated miRNAs in glioblastoma. Red miRNAs are downregulated while the green ones are upregulated in Glioblastoma cells.

miR-34a and miR-34a-5p function as tumor-suppressive miRNAs, inhibiting cell proliferation, cell-cycle progression, and cell invasion by targeting Notch1, Notch2, c-Met, CDK6, and EGFR [116,117]. Di Bari et al. reported that miR-34a-5p expression levels are inversely correlated to Notch1 and Notch2 expression, and its function is restored by the activation of M2 acetylcholine muscarinic receptors, which in turn downregulate Notch1 and consequently cell proliferation [117]. Wu et al. showed that lower levels of miR-34c-3p and miR-34c-5p correlate with a higher glioma grade. The overexpression of both miRNAs strongly inhibits glioma invasion and miR-34c-3p but not miR-34c-5p, promotes S-phase arrest, increases cell apoptosis, and reduces Notch2 expression [118]. Notch2 is a target of another tumor-suppressive miRNA, miR-181c, which reduces cell proliferation, cell invasion, and self-renewal capacities through Notch2 downregulation. Unfortunately, miR-181c is commonly downregulated in Glioblastoma, especially in the mesenchymal subtype, suggesting a potential relationship between miR-181c and the malignant behavior of Glioblastoma [119]. Among the miRNAs associated with shorter survival in Glioblastoma, Wong et al. discovered miR-148a and miR-31 [120]. miR-148a is frequently upregulated in Glioblastoma and correlated with hypoxia-induced and extracellular-matrix genes, while high levels of miR-31 are appreciated only in a small group of Glioblastomas and are associated with proliferation and immune-response genes. A common target of both miRNAs is factor-inhibiting HIF-1 (FIH1), which mediates their effects on tumor growth, counteracting HIF-1α and the NICD. In particular, HIF-1α is able to stabilize the NICD in order to expand and maintain GSCs. The inhibition of miR-148a and miR-31 in Glioblastoma mouse models prolongs animal survival, depletes the stem cell pool, suppresses tumor growth, and normalizes tumor vasculature [120]. With regard to GSC plasticity, miR-18a is a key player in controlling the switch between the self-renewing and non-self-renewing states [121]. By downregulating Dll3 and strengthening Notch1 signaling, miR-18a induces the expression of SHH and GLI-1 via ERK, maintaining the self-renewal and stemness abilities of GSCs [121]. The last investigated Notch-related miRNA in Glioblastoma is miR-33a, which promotes GSC growth and is responsible for their self-renewal abilities. This occurs because, among miR-33a target genes, there is a UV-radiation resistance-associated gene (UVRAG) that negatively regulates the Notch signaling pathway through the repression of Notch endocytosis. Moreover, an inverse correlation between the expression levels of miR-33a and UVRAG exists in Glioblastoma, and patients with a higher expression of miR-33a are characterized by poor prognosis and shorter survival [122].

5.4. Tumorigenesis and Other Tumor Aspects Driven by Notch Pathway

The overexpression of Notch1 promotes AKT activation, which in turn induces the nuclear localization of β-catenin and NF-κB, together with the Notch-mediated overexpression of Snail, Zeb1, and vimentin, promotes cell invasion and migration (Figure 4) [98]. The crosstalk between Notch and AKT is also mediated by collapsine response mediator protein-5 (CRMP5), preventing Notch degradation and favoring Glioblastoma proliferation [123]. In addition, CMRP5high Glioblastoma has elevated Hey1 expression compared to CMRP5low Glioblastoma, suggesting CMRP5 as an indicator of poor survival [123]. Two transient receptor cation channels, TRPM7 and TRPC6, are linked to Notch pathway-stimulating proliferation, invasion, and the migration of glioma cells [124,125]. In particular, TRPM7 levels correlate with those of Notch1, Jagged1, Hey2, and survivin [124]. Neurosphere cultures with high Notch1 levels show a more infiltrative phenotype when compared to Notch1low cultures [126]; furthermore, the suppression of cell migration, tumor invasion, and angiogenesis can be achieved by targeting the urokinase-type plasminogen activator/urokinase-type plasminogen activator receptor (uPA/uPAR) system in order to inhibit Notch-signaling-induced AKT, NF-κB, and ERK pathways [127]. The oncogenic role of Notch1 can also be due to the infection of human cytomegalovirus, which upregulates Notch1, ATF5 (an anti-apoptotic protein already highly expressed in Glioblastoma), and stem cell markers CD133, nestin, SOX2, OCT4, KLF4, and BMI-1 [128]. Finally, Wang et al. reported that silencing Notch1 reduced GSC proliferation and oncogenicity in vitro and in vivo [129].

Figure 4.

Figure 4

Other Notch-related tumorigenic aspects in the pathogenesis of Glioblastoma.

Besides Notch1, even Notch2 overexpression enhances cell migration in an RBPJK-dependent manner [130]. Notch2 deregulation might also promote NSC transformation and gliomagenesis, preventing neuronal lineage [103]. The stem cell phonotype is also supported and maintained by stanniocalcin-1 (STC1) and LMO2. STC1, a secretory glycoprotein, is highly expressed in glioma spheres; it is able to bind Notch1 and activate the Notch1-SOX2 signaling pathway, therefore supporting the stemness and tumorigenicity of GSCs [131]. Transcription factor LMO2, besides being inversely correlated with shorter survival, promotes the endothelial-like conversion of GSCs through the activation of VE-cadherin [132]. Jeon et al. demonstrated that inhibitor of differentiation 4 (ID4) increases Jagged1 expression, followed by Notch1 activation to drive astrocytes into a neural stem-like cell state and to increase cyclin E to produce a hyperproliferative state [133]. The downregulation of E3 ubiquitin ligase TRIM3 and the high levels of Musashi found in Glioblastoma promote the growth, survival, and self-renewal of stem cells and differentiation through the Numb/Notch pathway [134].

The tumor microenvironment also contributes to tumorigenesis. Endothelial cells function as a CSC niche by providing Notch ligands to Notch receptors on GSCs. The absence of Notch ligands on endothelial cells can reduce the CD133+ glioma subpopulation in vitro, inhibiting neurosphere propagation [135]. Moreover, differentiated cells within the tumor express higher levels of Dll1 compared to GSCs, contributing to Notch signaling activation in GSCs [135]. Similarly, silenced DLL1 in GSCs decreases stem cell markers and impairs self-renewal ability [136]. On the contrary, mesenchymal stem cells (MSCs) have a tumor-suppressor effect on GSCs. Indeed, paracrine signals from MSCs sensitize NCH421k and NCH644 cell lines to TMZ, probably turning them toward more differentiated cell types, downregulating Notch1 and SOX2 and upregulating vimentin and GFAP [137].

Under hypoxic conditions, GSCs increases the expression of several Notch genes (NOTCH1, NOTCH3, DLL1, JAGGED1, JAGGED2, HES1, HEY1, and HEY2), and hypoxia-related genes (HIF-1α, VEGF, LOX, and HIG2) [125,138,139]. The hypoxia-Notch gene subset might hold a prognostic implication, as the overexpression of the hypoxia-Notch axis is associated with poor survival [138]. Notch1 activation under hypoxic conditions also induces the expression of transient receptor TRCP6, which has emerged as a critical player in Glioblastoma aggressiveness, promoting NFAT activity, a crucial factor for glioma proliferation [125]. Han et al. showed that Notch1 inhibition in Glioblastoma xenografts reduces the hypoxic fraction and delays tumor growth, further supporting the crosstalk between Notch signaling and hypoxia, suggesting a potential mechanism whereby Notch1 downregulation radiosensitizes Glioblastoma cells [100]. On the contrary, Bayin et al. demonstrated that the NICD is strongly expressed in perivascular tumor regions and not in the hypoxic zone. However, the authors highlighted an intratumoral GSC heterogeneity with a divergent activation of Notch signaling, which coexist in tumors, but populates distinct niches and accordingly organizes their metabolisms [140]. Charles et al. showed that endothelial nitric oxide (eNOS) maintains the GSC phenotype in perivascular niches, activating the Notch pathway via paracrine signaling and promoting in vivo tumorigenicity [141]. In another report, the convergence of the PDGF-Notch-NO axis simultaneously drove perivascular promotion of the GSC phenotype and angiogenesis [142]. Jubb et al. reported the presence of Dll4 and Jagged1 in Glioblastoma vasculature [143]. These data define Glioblastoma subsets that might be sensitive (Dll4+/Jagged1+) or resistant (Dll4+/Jagged1-) to bevacizumab, a humanized monoclonal antibody against VEGF [143]. It was also reported that the Wnt signaling pathway represses Notch signaling in a Glioblastoma hypoxic microenvironment and supports the ablation of CD133+ subpopulations [144], which can populate tumors regardless of local vascularity and selectively utilize anaerobic glycolysis to expand in hypoxia [140]. Moreover, Notch1-stimulated GSCs induce highly vascularized tumors in vivo, with the production of several angiogenesis-related factors and the expression of pericyte cell markers [92].

Finally, the FOXG1/TLE1 complex cooperates with Notch signaling to promote gliomagenesis by directly repressing CHAC1 expression, a negative regulator of Notch3. Dali et al. also identified DNER as an additional potential transcription-repression target of FOXG1/TLE1: DNER inhibits Glioblastoma-derived neurosphere growth and promotes their differentiation, opposite to the effect of FOXG1 and TLE1 [145].

5.5. Therapeutic Approaches against Notch Signaling in Glioblastoma

To date, several classes of Notch inhibitors have been developed (Table 2). The most employed in cancer are γ-secretase inhibitors (GSIs), which prevent the release of the active NICD from the receptor by the γ-secretase complex, while α-secretase inhibitors (ASIs), which inhibit members of the ADAM family by preventing the second cleavage (S2) of the Notch receptor, are less used. Finally, to overcome GSI/ASI resistance, new therapeutic approaches that directly or indirectly target Notch signaling have been developed.

Table 2.

Therapeutic approaches against Notch signaling in Glioblastoma.

Class of Inhibitors Molecules Biological Effects
Decreased Cell Growth Anti-Proliferative Activity Pro-Apoptotic Activity Reduced Neurosphere Formation Reduced Colony Formation Decreased Tumor Size In Vivo Prolonged Animal Survival Others
γ-Secretase inhibitor (GSI) DAPT
(GSI-IX)
• Amplifies the effects of radiation
• Induces differentiation
• In combination with Iressa, reduces VEGF
LLNle
L-685,458
RO4929097 • In combination with RT and TMZ, reduces CD133, SOX2, and nestin expression
BMS-708163
MRK003 • Sensitizes cells to TMZ
• Reduces onco-metabolite levels
• In combination with GSI-XVIII, reduces CD133, nestin, OLIG2, and BMI-1 expression, and neurosphere formation
GSI-XVIII
GSI-X • No effects on cell-cycle distribution, apoptosis, and cell invasion
α-Secretase inhibitor (ASI) INCB3619 • In combination with DAPT, represses HES1 and HEY1 expression
Others Arsenic trioxide (ATO) • Enhances radiation-induced killing of Glioblastoma cells
• Decreases Notch1, Hes1, nestin, and CD133 levels
Tipifarnib • Sensitizes GSCs to GSIs
• Combined treatment with RO4929097 sensitizes cells to radiation and reduces in vivo tumor growth
Honokiol • Combined treatment with o6-BG increases TMZ sensitivity and suppresses Notch3 and Hes1 mRNA levels
mAb428.2 • Blocks ADAM17 activation
• Enhancement of inflammatory macrophage infiltration
• Reduces in vivo vascularization
ZR30 • Prevents MMP2 activation
• Limits tumor invasion
• Blocks EGFR/Notch/AKT signaling
dnMAML peptide • Reduces Hes1 and Hey3 expression
Retinoic acid • Downregulates the Hes and Hey family
• Induces neural differentiation
aPKC-PSP • Reduces Notch1 levels

5.5.1. γ-Secretase Inhibitors

DAPT (GSI-IX), the most known and used GSI, amplifies the effect of radiation, and reduces GSC proliferation and the number of endothelial cells disrupting the perivascular niche [146]. The suppression of cell proliferation and induction of apoptosis is mediated by the decrease of NF-κB (p65) expression through Notch inhibition [105]. Glioblastoma neurospheres with high Notch activity are more sensitive to DAPT and DAPT-treated cultures and show a more differentiated state and low Hes5 levels [126,146,147]. The combined treatment of DAPT with Iressa, an EGFR inhibitor, reduces VEGF expression and secretion when compared to single treatments. Unfortunately, this combined treatment is not sufficient to fully block endothelial cells from sprouting, probably due to other angiogenesis factors. Moreover, by blocking EGFR signaling, Hes1 levels decreased, suggesting that EGFR signaling stimulates Notch pathway activity [148]. Like DAPT, LLNle and L-685,458 are also able to kill GSCs, inhibiting NICD generation and inducing proteasome inhibition, proteolytic stress, and mitotic arrest [149]. DAPT and L-685,458 strongly reduce neurosphere formation in Glioblastoma cell lines, although DAPT and L-685,458 work to a much lesser extent than LLNle [149,150]. GSI RO4929097, in combination with radiation and TMZ, decreases the expression of CD133, SOX2, and nestin (inducing neural and astrocytic differentiation), has an anti-proliferative effect (reducing 3D spheroid growth), and increases the survival of the orthotopic Glioblastoma mouse model [151,152]. The triple combination is more effective than radio- and chemotherapy, or GSI alone [152]. Notably, Saito et al. showed that GSCs sensitive to DAPT, RO4929097, and BMS-708163, another GSI, have a gene signature enriched in proneural genes such as OLIG2, SOX2, ERBB3, HDAC2, TGFB3, DLL3, CHIL3I, and NKX2-2 [151].

MRK003 reduces cell growth in vitro and in vivo and sensitizes cell lines and neurospheres to radiation and TMZ [153]. It was demonstrated that MRK003 has a strong therapeutic potential in CD44high/CD133low GICs [150]. Intriguingly, Kahlert et al., through the metabolomic analysis of MRK003-treated Glioblastoma neurospheres, found reduced levels of intracellular glutamate, glutaminase (known to promote cancer cell proliferation), phosphocoline (which is elevated in fast-dividing glioma cells and in malignant high-grade brain tumors), and glycine (involved in the survival regulation of hypoxic glioma cells) [154]. However, MRK003 and GSI-XVII reduce the expression of stemness markers, such as CD133, nestin, BMI-1, and OLIG2, concomitant with the reduction of neurosphere formation and clonogenicity ability in vitro and in vivo. Moreover, both GSIs increase apoptosis, reducing AKT and STAT3 phosphorylation [150,155], and GSCs pretreated with MRK003 and GSI-XVIII show reduced tumor formation in vivo. In order to avoid GSI gastrointestinal toxicity, Fan et al. administered inhibitors through the brain implantation of drug-impregnated polymer beads that effectively blocked tumor growth and significantly prolonged animal survival [155].

Finally, GSI-X significantly impairs c-CSC cell growth compared with p-CSC pools, with no effects observed in cell-cycle distribution, apoptosis, and cell-invasion assays [156].

In conclusion, GSIs, being pan-Notch inhibitors, cause intestinal toxicity through the goblet cell metaplasia of the small intestinal epithelium. To limit toxicity, a preclinical study using antibodies for specific Notch receptor has shown that inhibition of the Notch1 receptor alone induces mild goblet cell metaplasia, whereas the inhibition of Notch2 receptor alone can eliminate this effect [157]

5.5.2. α-Secretase Inhibitors

In the literature, only one ASI has been used in Glioblastoma. Floyd et al. found that INCB3619 decreases cell growth and tumor size and prolongs the survival of a Glioblastoma animal model [158]. Moreover, combined treatment with DAPT represses HES1 and HEY1 expression, as well as LIF and YKL-40 levels, two new key players in Glioblastoma pathogenesis [158,159].

5.5.3. Other Molecules

Besides ASIs and GSIs, other molecules have been employed to block Notch signaling in Glioblastoma with the aim of overcoming side effects and resistance to GSIs.

Arsenic trioxide (ATO) is an inorganic compound that was approved by the Food and Drug Administration (FDA) in 2000 for the treatment of acute promyelocytic leukemia (APL) because of its strong anti-growth APL-derived stem cells [160]. With this rationale, Zhen et al. discovered that ATO reduces colony formation and nestin expression, induces apoptosis, and enhances the radiation-induced killing of Glioblastoma cells by decreasing Notch1 and Hes1 protein levels [161]. These data were confirmed by other studies, which further demonstrated that ATO treatment decreases CD133 expression and induces apoptosis through the repression of phosphorylation of AKT and STAT3 through the Notch pathway [162,163].

Tipifarnib, a farnesyltransferase inhibitor, sensitizes GSCs to GSIs, whereas the combined treatment with RO4929097 makes tumor cells more sensitive to radiation, resulting in significantly reduced tumor growth and improved survival in animal models. Intriguingly, non-stem GSCs are resistant to treatment, suggesting that combined treatment selectively targets the CSC pool [164].

Honokiol, a natural extract from different Magnolia species, can readily cross the blood-brain barrier and shows pro-apoptotic activity in Glioblastoma [165]. Combined treatment with O6-benzylguanine (O6-BG), an MGMT inhibitor, increases TMZ sensitivity and suppresses Notch3 and Hes1 mRNA levels with pro-apoptotic effects on GSCs [166].

Fibulin-3, an extracellular matrix glycoprotein, is highly expressed in Glioblastoma and functions as an autocrine/paracrine activator of NF-κB and the Notch pathway, promoting tumor invasion, angiogenesis, and drug resistance, besides being a marker of poor prognosis [167]. Nandhu et al. developed an antibody against fibulin-3 called mAb428.2, which is able to prevent the fibulin-3-activation of ADAM17, resulting in cell apoptosis, enhancement of inflammatory macrophage infiltration, reduced tumor growth and vascularization, and extended survival in Glioblastoma mouse models [167]. Always with regard to fibulin-3, Li et al. developed ZR30, an in vitro synthetized protein, based on the human fibulin-3 protein variant (ETSP), lacking the N-terminal signal peptide (responsible for extracellular exportation). ZR30 prevents MMP2 activation, thus limiting tumor invasion, blocking EGFR/Notch/AKT signaling, exerting an anti-tumor effect on different Glioblastoma cell subpopulations in a mouse model miming intratumor heterogeneity [168].

A dominant negative MAML peptide was developed in order to inhibit Notch signaling, and it was demonstrated that it has anti-proliferative and pro-apoptosis effects, decreasing Hes1 and Hey3 expression [153,169]. Hes and Hey family members are also downregulated by retinoic acid, which can inhibit sphere and colony formation, promote cell-growth arrest, and induce neural differentiation, both in vitro and in vivo [170].

Protein kinase C iota (PRKCi) is in close proximity with Notch1. In a study by Phillips et al., it was found that inhibiting PRKCi by aPKC-PSP results in lower Notch1 levels and increased GSC death [171].

5.6. Therapeutic Resistance to Notch Inhibitors

The elevated heterogeneity of Glioblastoma tissue is why the therapeutic approach fails. Concerning Notch-targeting strategies (Figure 5), it was shown that GSCs express higher levels of RBPJK compared to non-GSCs, and RBPJK knockdown reduced tumor propagation in vitro and in vivo [172]. Notably, RBPJK could regulate a different transcription program than Notch, binding CDK9 and therefore affecting Pol-II transcription elongation. Targeting CDK9, or even c-Myc, an upstream regulator of RBPJK, decreased the propagation of GSCs and prolonged survival in an orthotopic mouse model [172]. Another possible resistance mechanism is the overactivation of the Hedgehog pathway under Notch inhibition, which in turn increases GLI-1 expression via the inhibition of Hes1. Indeed, targeting both Notch and Hedgehog increases apoptosis, inhibiting cell growth and colony-forming ability more dramatically compared to monotherapy [173]. The upregulation of Dll4-Notch signaling might have a possible role in chemoresistance and be related to a classical vascular pattern, tumor edema, and MGMT-methylated promoter [97].

Figure 5.

Figure 5

Potential mechanisms involved in Notch-mediated therapeutic resistance.

High CBF1 levels are also implicated in drug resistance. Maciaczyk et al. found that targeting CBF1 impairs glioma invasion, suppressing Zeb1, an activator of the epithelial-mesenchymal transition program, and sensitizes cells to drugs other than GSIs. Unfortunately, it was reported that a CBF1 blockade can activate Notch target genes [174].

It was also reported that ionizing radiation induces Notch pathway activation, resulting in GSC expansion, whereas Notch inhibition sensitizes GSCs, but not non-GSCs, to radiotherapy [175]. Similarly, CD133high GSCs were resistant to MRK003 treatment due the high level of drug-resistance genes, such as BCRP1 [176]. It was also demonstrated that numerous patient-derived GSCs, responding to Notch inhibition, express high levels of ASCL1, a proneural transcription factor involved in normal neurogenesis. ASCL1high GSCs exhibit a latent capacity for terminal neuronal differentiation in response to Notch signaling inhibition, whereas ASCL1low GSCs do not [177].

Finally, Nastumeda et al. found that MRK003 promotes autophagy in GSCs, as revealed by the higher expression of LC3B-II/LC3B-I autophagy markers after treatment, resulting in chemoresistance induction. Fortunately, this can be avoided by treating cells with autophagy inhibitors together with GSIs [178].

5.7. Glioblastoma Clinical Studies and Notch Inhibitors

In the last decade, several clinical trials have been conducted to evaluate the dose-limiting toxicity (DLT) and tumor-suppressive effects of Notch inhibitors in Glioblastoma patients. Despite different clinical studies being conducted, only two molecules were tested: RO4929097, a γ-secretase inhibitor, and CB-103, a novel first-in-class orally active small-molecule inhibitor of the Notch transcriptional activation complex in the nucleus [179].

Targeting recurrent or progressive Glioblastoma patients with GSI RO4929097 (NCT01122901; https://clinicaltrials.gov/ct2/show/NCT01122901) showed that the six-month and two-year progression-free survival was 1.7 months, while OS (two years) was 6.7 months. Moreover, according to the response assessment in the neuro-oncology (RANO) criteria, the majority of enrolled patients presented disease progression, one complete remission, and three occasions of stable disease. The lack of activity by RO4929097 is probably due to its auto-induction metabolism, resulting in an increased CYP3A4 activity and a marked reduction of steady-state drug levels [180].

Since the standard of care for glioblastoma patients is radiotherapy followed by chemotherapy, a phase 0/I was conducted to study the effect of chemo-radiotherapy in combination with RO4929097 in newly diagnosed malignant gliomas (NCT01119599; https://clinicaltrials.gov/ct2/show/NCT01119599). The combined regimen was well-tolerated and no dose-limiting toxicities were observed. The study showed a significant decrease in proliferation and NICD expression by tumor cells and blood vessels, with a concomitant reduction of the CD133+ GSC subpopulation. Investigators also reported alterations in the angiogenesis pathways under treatment, although the tumor was able to adapt to perturbations in the microenvironment, switching to a Notch-independent form of angiogenesis, highlighting the necessity of concomitantly targeting multiple signaling pathways in Glioblastoma [181]. Angiogenesis is the issue of another study (NCT01189240; https://clinicaltrials.gov/ct2/show/NCT01189240), in which the combined treatment of RO4929097 with bevacizumab on patients with progressive or recurrent malignant glioma was evaluated. The drug combination was well-tolerated, and two (both Glioblastoma) of 12 evaluable subjects showed a radiographic response with complete or partial remission, although bevacizumab has often been associated with an invasive phenotype [182].

Finally, a new clinical trial (phase I/IIA) began in January 2018 (NCT03422679; https://clinicaltrials.gov/ct2/show/record/NCT03422679). In this study, CB-103 was employed to treat several advanced or metastatic solid tumors and haematological malignancies, including Glioblastoma. The current primary outcomes are to evaluate DLT and antitumor efficacy. The study is ongoing.

6. Conclusions

Glioblastoma is the most aggressive types of brain tumor, with a final mortality rate close to 100%. Conventional therapies have failed to improve patient survival due to a small subpopulation of cancer cells known as GSCs that exhibit an enhanced self-renewal capacity, compromised differentiation, in vivo tumorigenicity, and resistance to radio- and chemotherapy. Blocking certain pathways can be useful to induce GSC terminal differentiation and apoptosis in order to reduce their survival. Among them, the Notch signaling pathway is one of the most important. The dysregulation of Notch signaling can take place at different levels, involving genetic, epigenetic, miRNA, and protein regulation in order to maintain cells in an undifferentiated state. Therefore, the Notch pathway could represent an attractive target for treatment in order to induce cell differentiation and kill both undifferentiated and differentiated tumor cells. The most employed and effective inhibitors in Glioblastoma are GSIs, which inhibit the activity of the γ-secretase complex, resulting in no formation of the active Notch form. Even if Notch-targeted monotherapy seems to be promising, a better accomplishment is to combine Notch inhibitors with other molecules or chemotherapeutic agents like bevacizumab and TMZ, respectively. This is because Notch signaling is at the center of a signaling network, which also includes pathways such as PI3K/AKT, NF-κB, STAT3, Hedgehog, and Wnt/β-catenin, which are involved in cell differentiation, cell growth, and survival. Although effective, GSIs present many several side effects, like acute gastrointestinal toxicity. Thus, new compounds or other administration routes should be developed to avoid side effects. To further complicate the possibility of achieving satisfactory results in patient treatment, no specific Notch-related biomarkers are available for Notch-targeted treatment selection and/or treatment response. This situation suggests that patients might be exposed to an ineffective therapeutic regimen, with consequent side effects and higher costs for health institutions.

In conclusion, the Notch pathway is definitely involved in Glioblastoma tumorigenesis, even if its role seems to be quite controversial. However, Notch-signaling inhibition represents a potential therapeutic approach to kill tumor cells, especially in combination with other molecules. Further investigations are needed to better understand the molecular mechanisms of Notch signaling in Glioblastoma pathogenesis, and how they can be overcome to develop an effective therapeutic approach against Notch to improve patient survival.

Author Contributions

R.B. wrote the manuscript and prepared the figures and tables. A.B. critically revised the manuscript. Both authors approved the submitted version of the manuscript and agreed to be personally accountable for their own contribution.

Funding

This research received no external funding.

Conflicts of Interest

The authors declare no conflict of interest.

References

  • 1.Seymour T., Nowak A., Kakulas F. Targeting Aggressive Cancer Stem Cells in Glioblastoma. Front. Oncol. 2015;5:159. doi: 10.3389/fonc.2015.00159. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 2.Louis D.N., Perry A., Reifenberger G., von Deimling A., Figarella-Branger D., Cavenee W.K., Ohgaki H., Wiestler O.D., Kleihues P., Ellison D.W. The 2016 World Health Organization Classification of Tumors of the Central Nervous System: A summary. Acta Neuropathol. 2016;131:803–820. doi: 10.1007/s00401-016-1545-1. [DOI] [PubMed] [Google Scholar]
  • 3.Clarke J., Butowski N., Chang S. Recent advances in therapy for glioblastoma. Arch. Neurol. 2010;67:279–283. doi: 10.1001/archneurol.2010.5. [DOI] [PubMed] [Google Scholar]
  • 4.Wilson T.A., Karajannis M.A., Harter D.H. Glioblastoma multiforme: State of the art and future therapeutics. Surg. Neurol. Int. 2014;5:64. doi: 10.4103/2152-7806.132138. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 5.Roy S., Lahiri D., Maji T., Biswas J. Recurrent Glioblastoma: Where we stand. South Asian J. Cancer. 2015;4:163–173. doi: 10.4103/2278-330X.175953. [DOI] [PMC free article] [PubMed] [Google Scholar] [Retracted]
  • 6.Baronchelli S., Bentivegna A., Redaelli S., Riva G., Butta V., Paoletta L., Isimbaldi G., Miozzo M., Tabano S., Daga A., et al. Delineating the cytogenomic and epigenomic landscapes of glioma stem cell lines. PLoS ONE. 2013;8:e57462. doi: 10.1371/journal.pone.0057462. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 7.Sottoriva A., Spiteri I., Piccirillo S.G., Touloumis A., Collins V.P., Marioni J.C., Curtis C., Watts C., Tavare S. Intratumor heterogeneity in human glioblastoma reflects cancer evolutionary dynamics. Proc. Natl. Acad. Sci. USA. 2013;110:4009–4014. doi: 10.1073/pnas.1219747110. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 8.Vescovi A.L., Galli R., Reynolds B.A. Brain tumour stem cells. Nat. Rev. Cancer. 2006;6:425–436. doi: 10.1038/nrc1889. [DOI] [PubMed] [Google Scholar]
  • 9.Alifieris C., Trafalis D.T. Glioblastoma multiforme: Pathogenesis and treatment. Pharmacol. Ther. 2015;152:63–82. doi: 10.1016/j.pharmthera.2015.05.005. [DOI] [PubMed] [Google Scholar]
  • 10.Bradshaw A., Wickremsekera A., Tan S.T., Peng L., Davis P.F., Itinteang T. Cancer Stem Cell Hierarchy in Glioblastoma Multiforme. Front. Surg. 2016;3:21. doi: 10.3389/fsurg.2016.00021. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 11.Ahmed A.U., Auffinger B., Lesniak M.S. Understanding glioma stem cells: Rationale, clinical relevance and therapeutic strategies. Expert Rev. Neurother. 2013;13:545–555. doi: 10.1586/ern.13.42. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 12.Guruharsha K.G., Kankel M.W., Artavanis-Tsakonas S. The Notch signalling system: Recent insights into the complexity of a conserved pathway. Nat. Rev. Genet. 2012;13:654–666. doi: 10.1038/nrg3272. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 13.Andersson E.R., Sandberg R., Lendahl U. Notch signaling: Simplicity in design, versatility in function. Development. 2011;138:3593–3612. doi: 10.1242/dev.063610. [DOI] [PubMed] [Google Scholar]
  • 14.Yang X., Klein R., Tian X., Cheng H.T., Kopan R., Shen J. Notch activation induces apoptosis in neural progenitor cells through a p53-dependent pathway. Dev. Biol. 2004;269:81–94. doi: 10.1016/j.ydbio.2004.01.014. [DOI] [PubMed] [Google Scholar]
  • 15.Hori K., Sen A., Artavanis-Tsakonas S. Notch signaling at a glance. Pt 10J. Cell Sci. 2013;126:2135–2140. doi: 10.1242/jcs.127308. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 16.Del Alamo D., Rouault H., Schweisguth F. Mechanism and significance of cis-inhibition in Notch signalling. Curr. Biol. 2011;21:R40–R47. doi: 10.1016/j.cub.2010.10.034. [DOI] [PubMed] [Google Scholar]
  • 17.LeBon L., Lee T.V., Sprinzak D., Jafar-Nejad H., Elowitz M.B. Fringe proteins modulate Notch-ligand cis and trans interactions to specify signaling states. Elife. 2014;3:e02950. doi: 10.7554/eLife.02950. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 18.Kopan R., Ilagan M.X. The canonical Notch signaling pathway: Unfolding the activation mechanism. Cell. 2009;137:216–233. doi: 10.1016/j.cell.2009.03.045. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 19.Wang M.M. Notch signaling and Notch signaling modifiers. Int. J. Biochem. Cell Biol. 2011;43:1550–1562. doi: 10.1016/j.biocel.2011.08.005. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 20.Chillakuri C.R., Sheppard D., Lea S.M., Handford P.A. Notch receptor–ligand binding and activation: Insights from molecular studies. Semin. Cell Dev. Biol. 2012;23:421–428. doi: 10.1016/j.semcdb.2012.01.009. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 21.Fiuza U.M., Arias A.M. Cell and molecular biology of Notch. J. Endocrinol. 2007;194:459–474. doi: 10.1677/JOE-07-0242. [DOI] [PubMed] [Google Scholar]
  • 22.Logeat F., Bessia C., Brou C., LeBail O., Jarriault S., Seidah N.G., Israël A. The Notch1 receptor is cleaved constitutively by a furin-like convertase. Proc. Natl. Acad. Sci. USA. 1998;95:8108–8112. doi: 10.1073/pnas.95.14.8108. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 23.Zolkiewska A. ADAM proteases: Ligand processing and modulation of the Notch pathway. Cell. Mol. Life Sci. 2008;65:2056–2068. doi: 10.1007/s00018-008-7586-4. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 24.Mumm J.S., Schroeter E.H., Saxena M.T., Griesemer A., Tian X., Pan D.J., Ray W.J., Kopan R. A ligand-induced extracellular cleavage regulates gamma-secretase-like proteolytic activation of Notch1. Mol. Cell. 2000;5:197–206. doi: 10.1016/S1097-2765(00)80416-5. [DOI] [PubMed] [Google Scholar]
  • 25.Fortini M.E. Gamma-secretase-mediated proteolysis in cell-surface-receptor signalling. Nat. Rev. Mol. Cell Biol. 2002;3:673–684. doi: 10.1038/nrm910. [DOI] [PubMed] [Google Scholar]
  • 26.Zanotti S., Canalis E. Notch Signaling and the Skeleton. Endocr. Rev. 2016;37:223–253. doi: 10.1210/er.2016-1002. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 27.De Strooper B., Annaert W., Cupers P., Saftig P., Craessaerts K., Mumm J.S., Schroeter E.H., Schrijvers V., Wolfe M.S., Ray W.J., et al. A presenilin-1-dependent gamma-secretase-like protease mediates release of Notch intracellular domain. Nature. 1999;398:518–522. doi: 10.1038/19083. [DOI] [PubMed] [Google Scholar]
  • 28.Wallberg A.E., Pedersen K., Lendahl U., Roeder R.G. p300 and PCAF act cooperatively to mediate transcriptional activation from chromatin templates by notch intracellular domains in vitro. Mol. Cell. Biol. 2002;22:7812–7819. doi: 10.1128/MCB.22.22.7812-7819.2002. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 29.Kurooka H., Honjo T. Functional Interaction between the Mouse Notch1 Intracellular Region and Histone Acetyltransferases PCAF and GCN5. J. Biol. Chem. 2000;275:17211–17220. doi: 10.1074/jbc.M000909200. [DOI] [PubMed] [Google Scholar]
  • 30.Wu L., Griffin J.D. Modulation of Notch signaling by mastermind-like (MAML) transcriptional co-activators and their involvement in tumorigenesis. Semin. Cancer Biol. 2004;14:348–356. doi: 10.1016/j.semcancer.2004.04.014. [DOI] [PubMed] [Google Scholar]
  • 31.Jarriault S., Brou C., Logeat F., Schroeter E.H., Kopan R., Israel A. Signalling downstream of activated mammalian Notch. Nature. 1995;377:355–358. doi: 10.1038/377355a0. [DOI] [PubMed] [Google Scholar]
  • 32.Iso T., Sartorelli V., Chung G., Shichinohe T., Kedes L., Hamamori Y. HERP, a new primary target of Notch regulated by ligand binding. Mol. Cell. Biol. 2001;21:6071–6079. doi: 10.1128/MCB.21.17.6071-6079.2001. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 33.Capaccione K.M., Pine S.R. The Notch signaling pathway as a mediator of tumor survival. Carcinogenesis. 2013;34:1420–1430. doi: 10.1093/carcin/bgt127. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 34.Teodorczyk M., Schmidt M.H.H. Notching on Cancer’s Door: Notch Signaling in Brain Tumors. Front. Oncol. 2014;4:341. doi: 10.3389/fonc.2014.00341. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 35.Yin L., Velazquez O.C., Liu Z.J. Notch signaling: Emerging molecular targets for cancer therapy. Biochem. Pharmacol. 2010;80:690–701. doi: 10.1016/j.bcp.2010.03.026. [DOI] [PubMed] [Google Scholar]
  • 36.Borggrefe T., Oswald F. The Notch signaling pathway: Transcriptional regulation at Notch target genes. Cell. Mol. Life Sci. 2009;66:1631–1646. doi: 10.1007/s00018-009-8668-7. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 37.Kopan R. Notch signaling. Cold Spring Harb. Perspect. Biol. 2012;4:a011213. doi: 10.1101/cshperspect.a011213. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 38.Andersen P., Uosaki H., Shenje L.T., Kwon C. Non-canonical Notch signaling: Emerging role and mechanism. Trends Cell Biol. 2012;22:257–265. doi: 10.1016/j.tcb.2012.02.003. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 39.Sanalkumar R., Dhanesh S.B., James J. Non-canonical activation of Notch signaling/target genes in vertebrates. Cell. Mol. Life Sci. 2010;67:2957–2968. doi: 10.1007/s00018-010-0391-x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 40.D’Souza B., Meloty-Kapella L., Weinmaster G. Canonical and Non-Canonical Notch Ligands. Curr. Top. Dev. Biol. 2010;92:73–129. doi: 10.1016/S0070-2153(10)92003-6. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 41.Mathieu P., Adami P.V., Morelli L. Notch signaling in the pathologic adult brain. Biomol. Concepts. 2013;4:465–476. doi: 10.1515/bmc-2013-0006. [DOI] [PubMed] [Google Scholar]
  • 42.Imayoshi I., Sakamoto M., Yamaguchi M., Mori K., Kageyama R. Essential roles of Notch signaling in maintenance of neural stem cells in developing and adult brains. J. Neurosci. 2010;30:3489–3498. doi: 10.1523/JNEUROSCI.4987-09.2010. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 43.Basak O., Giachino C., Fiorini E., MacDonald H.R., Taylor V. Neurogenic Subventricular Zone Stem/Progenitor Cells Are Notch1-Dependent in Their Active But Not Quiescent State. J. Neurosci. 2012;32:5654–5666. doi: 10.1523/JNEUROSCI.0455-12.2012. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 44.Taupin P. Adult neural stem cells, neurogenic niches, and cellular therapy. Stem Cell Rev. 2006;2:213–219. doi: 10.1007/s12015-006-0049-0. [DOI] [PubMed] [Google Scholar]
  • 45.Nyfeler Y., Kirch R.D., Mantei N., Leone D.P., Radtke F., Suter U., Taylor V. Jagged1 signals in the postnatal subventricular zone are required for neural stem cell self-renewal. EMBO J. 2005;24:3504–3515. doi: 10.1038/sj.emboj.7600816. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 46.Lugert S., Basak O., Knuckles P., Haussler U., Fabel K., Gotz M., Haas C.A., Kempermann G., Taylor V., Giachino C. Quiescent and active hippocampal neural stem cells with distinct morphologies respond selectively to physiological and pathological stimuli and aging. Cell Stem Cell. 2010;6:445–456. doi: 10.1016/j.stem.2010.03.017. [DOI] [PubMed] [Google Scholar]
  • 47.Sestan N., Artavanis-Tsakonas S., Rakic P. Contact-dependent inhibition of cortical neurite growth mediated by notch signaling. Science. 1999;286:741–746. doi: 10.1126/science.286.5440.741. [DOI] [PubMed] [Google Scholar]
  • 48.Breunig J.J., Silbereis J., Vaccarino F.M., Sestan N., Rakic P. Notch regulates cell fate and dendrite morphology of newborn neurons in the postnatal dentate gyrus. Proc. Natl. Acad. Sci. USA. 2007;104:20558–20563. doi: 10.1073/pnas.0710156104. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 49.Stump G., Durrer A., Klein A.L., Lutolf S., Suter U., Taylor V. Notch1 and its ligands Delta-like and Jagged are expressed and active in distinct cell populations in the postnatal mouse brain. Mech. Dev. 2002;114:153–159. doi: 10.1016/S0925-4773(02)00043-6. [DOI] [PubMed] [Google Scholar]
  • 50.Carlen M., Meletis K., Goritz C., Darsalia V., Evergren E., Tanigaki K., Amendola M., Barnabe-Heider F., Yeung M.S., Naldini L., et al. Forebrain ependymal cells are Notch-dependent and generate neuroblasts and astrocytes after stroke. Nat. Neurosci. 2009;12:259–267. doi: 10.1038/nn.2268. [DOI] [PubMed] [Google Scholar]
  • 51.Irvin D.K., Zurcher S.D., Nguyen T., Weinmaster G., Kornblum H.I. Expression patterns of Notch1, Notch2, and Notch3 suggest multiple functional roles for the Notch-DSL signaling system during brain development. J. Comp. Neurol. 2001;436:167–181. doi: 10.1002/cne.1059. [DOI] [PubMed] [Google Scholar]
  • 52.Murphy P.A., Lam M.T., Wu X., Kim T.N., Vartanian S.M., Bollen A.W., Carlson T.R., Wang R.A. Endothelial Notch4 signaling induces hallmarks of brain arteriovenous malformations in mice. Proc. Natl. Acad. Sci. USA. 2008;105:10901–10906. doi: 10.1073/pnas.0802743105. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 53.Kurisu J., Fukuda T., Yokoyama S., Hirano T., Kengaku M. Polarized targeting of DNER into dendritic plasma membrane in hippocampal neurons depends on endocytosis. J. Neurochem. 2010;113:1598–1610. doi: 10.1111/j.1471-4159.2010.06714.x. [DOI] [PubMed] [Google Scholar]
  • 54.Irvin D.K., Nakano I., Paucar A., Kornblum H.I. Patterns of Jagged1, Jagged2, Delta-like 1 and Delta-like 3 expression during late embryonic and postnatal brain development suggest multiple functional roles in progenitors and differentiated cells. J. Neurosci. Res. 2004;75:330–343. doi: 10.1002/jnr.10843. [DOI] [PubMed] [Google Scholar]
  • 55.Yoon K.J., Koo B.K., Im S.K., Jeong H.W., Ghim J., Kwon M.C., Moon J.S., Miyata T., Kong Y.Y. Mind bomb 1-expressing intermediate progenitors generate notch signaling to maintain radial glial cells. Neuron. 2008;58:519–531. doi: 10.1016/j.neuron.2008.03.018. [DOI] [PubMed] [Google Scholar]
  • 56.Shutter J.R., Scully S., Fan W., Richards W.G., Kitajewski J., Deblandre G.A., Kintner C.R., Stark K.L. Dll4, a novel Notch ligand expressed in arterial endothelium. Genes Dev. 2000;14:1313–1318. [PMC free article] [PubMed] [Google Scholar]
  • 57.Alberi L., Liu S., Wang Y., Badie R., Smith-Hicks C., Wu J., Pierfelice T.J., Abazyan B., Mattson M.P., Kuhl D., et al. Activity-induced Notch signaling in neurons requires Arc/Arg3.1 and is essential for synaptic plasticity in hippocampal networks. Neuron. 2011;69:437–444. doi: 10.1016/j.neuron.2011.01.004. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 58.Lavado A., Lagutin O.V., Chow L.M.L., Baker S.J., Oliver G. Prox1 Is Required for Granule Cell Maturation and Intermediate Progenitor Maintenance During Brain Neurogenesis. PLoS Biol. 2010;8:e1000460. doi: 10.1371/journal.pbio.1000460. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 59.Miller A.C., Lyons E.L., Herman T.G. cis-Inhibition of Notch by endogenous Delta biases the outcome of lateral inhibition. Curr. Biol. 2009;19:1378–1383. doi: 10.1016/j.cub.2009.06.042. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 60.Borghese L., Dolezalova D., Opitz T., Haupt S., Leinhaas A., Steinfarz B., Koch P., Edenhofer F., Hampl A., Brustle O. Inhibition of notch signaling in human embryonic stem cell-derived neural stem cells delays G1/S phase transition and accelerates neuronal differentiation in vitro and in vivo. Stem Cells. 2010;28:955–964. doi: 10.1002/stem.408. [DOI] [PubMed] [Google Scholar]
  • 61.Guentchev M., Ronald D.G.M. Notch controls proliferation and differentiation of stem cells in a dose-dependent manner. Eur. J. Neurosci. 2006;23:2289–2296. doi: 10.1111/j.1460-9568.2006.04766.x. [DOI] [PubMed] [Google Scholar]
  • 62.Boareto M., Iber D., Taylor V. Differential interactions between Notch and ID factors control neurogenesis by modulating Hes factor autoregulation. Development. 2017;144:3465–3474. doi: 10.1242/dev.152520. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 63.Engler A., Rolando C., Giachino C., Saotome I., Erni A., Brien C., Zhang R., Zimber-Strobl U., Radtke F., Artavanis-Tsakonas S., et al. Notch2 Signaling Maintains NSC Quiescence in the Murine Ventricular-Subventricular Zone. Cell Rep. 2018;22:992–1002. doi: 10.1016/j.celrep.2017.12.094. [DOI] [PubMed] [Google Scholar]
  • 64.Than-Trong E., Ortica-Gatti S., Mella S., Nepal C., Alunni A., Bally-Cuif L. Neural stem cell quiescence and stemness are molecularly distinct outputs of the Notch3 signalling cascade in the vertebrate adult brain. Development. 2018;145:dev161034. doi: 10.1242/dev.161034. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 65.Faigle R., Song H. Signaling mechanisms regulating adult neural stem cells and neurogenesis. Biochim. Biophys. Acta. 2013;1830:2435–2448. doi: 10.1016/j.bbagen.2012.09.002. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 66.Hashimoto-Torii K., Torii M., Sarkisian M.R., Bartley C.M., Shen J., Radtke F., Gridley T., Šestan N., Rakic P. Interaction between Reelin and Notch signaling regulates neuronal migration in the cerebral cortex. Neuron. 2008;60:273–284. doi: 10.1016/j.neuron.2008.09.026. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 67.Sibbe M., Forster E., Basak O., Taylor V., Frotscher M. Reelin and Notch1 cooperate in the development of the dentate gyrus. J. Neurosci. 2009;29:8578–8585. doi: 10.1523/JNEUROSCI.0958-09.2009. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 68.Lewis J. Neurogenic genes and vertebrate neurogenesis. Curr. Opin. Neurobiol. 1996;6:3–10. doi: 10.1016/S0959-4388(96)80002-X. [DOI] [PubMed] [Google Scholar]
  • 69.Henrique D., Hirsinger E., Adam J., Le Roux I., Pourquie O., Ish-Horowicz D., Lewis J. Maintenance of neuroepithelial progenitor cells by Delta-Notch signalling in the embryonic chick retina. Curr. Biol. 1997;7:661–670. doi: 10.1016/S0960-9822(06)00293-4. [DOI] [PubMed] [Google Scholar]
  • 70.Dorsky R.I., Chang W.S., Rapaport D.H., Harris W.A. Regulation of neuronal diversity in the Xenopus retina by Delta signalling. Nature. 1997;385:67–70. doi: 10.1038/385067a0. [DOI] [PubMed] [Google Scholar]
  • 71.Lowell S. Notch signalling: You make me feel so glial. Curr. Biol. 2000;10:R595–R597. doi: 10.1016/S0960-9822(00)00636-9. [DOI] [PubMed] [Google Scholar]
  • 72.Hojo M., Ohtsuka T., Hashimoto N., Gradwohl G., Guillemot F., Kageyama R. Glial cell fate specification modulated by the bHLH gene Hes5 in mouse retina. Development. 2000;127:2515–2522. doi: 10.1242/dev.127.12.2515. [DOI] [PubMed] [Google Scholar]
  • 73.Satow T., Bae S.-K., Inoue T., Inoue C., Miyoshi G., Tomita K., Bessho Y., Hashimoto N., Kageyama R. The Basic Helix-Loop-Helix Gene hesr2 Promotes Gliogenesis in Mouse Retina. J. Neurosci. 2001;21:1265–1273. doi: 10.1523/JNEUROSCI.21-04-01265.2001. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 74.Patten B.A., Sardi S.P., Koirala S., Nakafuku M., Corfas G. Notch1 signaling regulates radial glia differentiation through multiple transcriptional mechanisms. J. Neurosci. 2006;26:3102–3108. doi: 10.1523/JNEUROSCI.4829-05.2006. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 75.Penton A.L., Leonard L.D., Spinner N.B. Notch signaling in human development and disease. Semin. Cell Dev. Biol. 2012;23:450–457. doi: 10.1016/j.semcdb.2012.01.010. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 76.Takebe N., Nguyen D., Yang S.X. Targeting notch signaling pathway in cancer: Clinical development advances and challenges. Pharmacol. Ther. 2014;141:140–149. doi: 10.1016/j.pharmthera.2013.09.005. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 77.Ranganathan P., Weaver K.L., Capobianco A.J. Notch signalling in solid tumours: A little bit of everything but not all the time. Nat. Rev. Cancer. 2011;11:338–351. doi: 10.1038/nrc3035. [DOI] [PubMed] [Google Scholar]
  • 78.Somasundaram K., Reddy S.P., Vinnakota K., Britto R., Subbarayan M., Nambiar S., Hebbar A., Samuel C., Shetty M., Sreepathi H.K., et al. Upregulation of ASCL1 and inhibition of Notch signaling pathway characterize progressive astrocytoma. Oncogene. 2005;24:7073–7083. doi: 10.1038/sj.onc.1208865. [DOI] [PubMed] [Google Scholar]
  • 79.Kanamori M., Kawaguchi T., Nigro J.M., Feuerstein B.G., Berger M.S., Miele L., Pieper R.O. Contribution of Notch signaling activation to human glioblastoma multiforme. J. Neurosurg. 2007;106:417–427. doi: 10.3171/jns.2007.106.3.417. [DOI] [PubMed] [Google Scholar]
  • 80.Zhang X.P., Zheng G., Zou L., Liu H.L., Hou L.H., Zhou P., Yin D.D., Zheng Q.J., Liang L., Zhang S.Z., et al. Notch activation promotes cell proliferation and the formation of neural stem cell-like colonies in human glioma cells. Mol. Cell. Biochem. 2008;307:101–108. doi: 10.1007/s11010-007-9589-0. [DOI] [PubMed] [Google Scholar]
  • 81.Phillips H.S., Kharbanda S., Chen R., Forrest W.F., Soriano R.H., Wu T.D., Misra A., Nigro J.M., Colman H., Soroceanu L., et al. Molecular subclasses of high-grade glioma predict prognosis, delineate a pattern of disease progression, and resemble stages in neurogenesis. Cancer Cell. 2006;9:157–173. doi: 10.1016/j.ccr.2006.02.019. [DOI] [PubMed] [Google Scholar]
  • 82.Jing Y., Han Z., Zhang S., Liu Y., Wei L. Epithelial-Mesenchymal Transition in tumor microenvironment. Cell Biosci. 2011;1:29. doi: 10.1186/2045-3701-1-29. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 83.Adams J.M., Strasser A. Is Tumor Growth Sustained by Rare Cancer Stem Cells or Dominant Clones? Cancer Res. 2008;68:4018–4021. doi: 10.1158/0008-5472.CAN-07-6334. [DOI] [PubMed] [Google Scholar]
  • 84.Venkatesh V., Nataraj R., Thangaraj G.S., Karthikeyan M., Gnanasekaran A., Kaginelli S.B., Kuppanna G., Kallappa C.G., Basalingappa K.M. Targeting Notch signalling pathway of cancer stem cells. Stem Cell Investig. 2018;5:5. doi: 10.21037/sci.2018.02.02. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 85.Dragu D.L., Necula L.G., Bleotu C., Diaconu C.C., Chivu-Economescu M. Therapies targeting cancer stem cells: Current trends and future challenges. World J. Stem Cells. 2015;7:1185–1201. doi: 10.4252/wjsc.v7.i9.1185. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 86.Dirks P.B. Brain tumor stem cells: The cancer stem cell hypothesis writ large. Mol. Oncol. 2010;4:420–430. doi: 10.1016/j.molonc.2010.08.001. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 87.Yuan X., Curtin J., Xiong Y., Liu G., Waschsmann-Hogiu S., Farkas D.L., Black K.L., Yu J.S. Isolation of cancer stem cells from adult glioblastoma multiforme. Oncogene. 2004;23:9392–9400. doi: 10.1038/sj.onc.1208311. [DOI] [PubMed] [Google Scholar]
  • 88.Nakamura Y., Sakakibara S.I., Miyata T., Ogawa M., Shimazaki T., Weiss S., Kageyama R., Okano H. The bHLH Gene Hes1 as a Repressor of the Neuronal Commitment of CNS Stem Cells. J. Neurosci. 2000;20:283–293. doi: 10.1523/JNEUROSCI.20-01-00283.2000. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 89.Gunther H.S., Schmidt N.O., Phillips H.S., Kemming D., Kharbanda S., Soriano R., Modrusan Z., Meissner H., Westphal M., Lamszus K. Glioblastoma-derived stem cell-enriched cultures form distinct subgroups according to molecular and phenotypic criteria. Oncogene. 2008;27:2897–2909. doi: 10.1038/sj.onc.1210949. [DOI] [PubMed] [Google Scholar]
  • 90.Ignatova T.N., Xing H., Kim T.M., Jung Y., Huang W., Yang Hong W., Song S., Park Peter J., Carroll Rona S., Johnson Mark D. Human cortical glial tumors contain neural stem-like cells expressing astroglial and neuronal markers in vitro. Glia. 2002;39:193–206. doi: 10.1002/glia.10094. [DOI] [PubMed] [Google Scholar]
  • 91.Jiang X., Xing H., Kim T.M., Jung Y., Huang W., Yang Hong W., Song S., Park Peter J., Carroll Rona S., Johnson Mark D. Numb Regulates Glioma Stem Cell Fate and Growth by Altering Epidermal Growth Factor Receptor and Skp1-Cullin-F-Box Ubiquitin Ligase Activity. Stem Cells. 2012;30:1313–1326. doi: 10.1002/stem.1120. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 92.Guichet P.O., Guelfi S., Teigell M., Hoppe L., Bakalara N., Bauchet L., Duffau H., Lamszus K., Rothhut B., Hugnot J.P. Notch1 Stimulation Induces a Vascularization Switch With Pericyte-Like Cell Differentiation of Glioblastoma Stem Cells. Stem Cells. 2014;33:21–34. doi: 10.1002/stem.1767. [DOI] [PubMed] [Google Scholar]
  • 93.Qiang L., Wu T., Zhang H.W., Lu N., Hu R., Wang Y.J., Zhao L., Chen F.H., Wang X.T., You Q.D., et al. HIF-1α is critical for hypoxia-mediated maintenance of glioblastoma stem cells by activating Notch signaling pathway. Cell Death Differ. 2011;19:284. doi: 10.1038/cdd.2011.95. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 94.Hu Y.-Y., Fu L.-A., Li S.-Z., Chen Y., Li J.-C., Han J., Liang L., Li L., Ji C.-C., Zheng M.-H., et al. Hif-1α and Hif-2α differentially regulate Notch signaling through competitive interaction with the intracellular domain of Notch receptors in glioma stem cells. Cancer Lett. 2014;349:67–76. doi: 10.1016/j.canlet.2014.03.035. [DOI] [PubMed] [Google Scholar]
  • 95.Hulleman E., Quarto M., Vernell R., Masserdotti G., Colli E., Kros J.M., Levi D., Gaetani P., Tunici P., Finocchiaro G., et al. A role for the transcription factor HEY1 in glioblastoma. J. Cell. Mol. Med. 2009;13:136–146. doi: 10.1111/j.1582-4934.2008.00307.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 96.Brennan C., Momota H., Hambardzumyan D., Ozawa T., Tandon A., Pedraza A., Holland E. Glioblastoma subclasses can be defined by activity among signal transduction pathways and associated genomic alterations. PLoS ONE. 2009;4:e7752. doi: 10.1371/journal.pone.0007752. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 97.El Hindy N., Keyvani K., Pagenstecher A., Dammann P., Sandalcioglu I.E., Sure U., Zhu Y. Implications of Dll4-Notch signaling activation in primary glioblastoma multiforme. Neuro Oncol. 2013;15:1366–1378. doi: 10.1093/neuonc/not071. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 98.Zhang X., Chen T., Zhang J., Mao Q., Li S., Xiong W., Qiu Y., Xie Q., Ge J. Notch1 promotes glioma cell migration and invasion by stimulating β-catenin and NF-κB signaling via AKT activation. Cancer Sci. 2011;103:181–190. doi: 10.1111/j.1349-7006.2011.02154.x. [DOI] [PubMed] [Google Scholar]
  • 99.Xing Z.Y., Sun L.G., Guo W.J. Elevated expression of Notch-1 and EGFR induced apoptosis in glioblastoma multiforme patients. Clin. Neurol. Neurosurg. 2015;131:54–58. doi: 10.1016/j.clineuro.2015.01.018. [DOI] [PubMed] [Google Scholar]
  • 100.Han N., Hu G., Shi L., Long G., Yang L., Xi Q., Guo Q., Wang J., Dong Z., Zhang M. Notch1 ablation radiosensitizes glioblastoma cells. Oncotarget. 2017;8:88059–88068. doi: 10.18632/oncotarget.21409. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 101.Biswas S., Rao C.M. Epigenetics in cancer: Fundamentals and Beyond. Pharmacol. Ther. 2017;173:118–134. doi: 10.1016/j.pharmthera.2017.02.011. [DOI] [PubMed] [Google Scholar]
  • 102.Dell’albani P., Rodolico M., Pellitteri R., Tricarichi E., Torrisi S.A., D’Antoni S., Zappia M., Albanese V., Caltabiano R., Platania N., et al. Differential patterns of NOTCH1-4 receptor expression are markers of glioma cell differentiation. Neuro Oncol. 2014;16:204–216. doi: 10.1093/neuonc/not168. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 103.Tchorz J.S., Tome M., Cloetta D., Sivasankaran B., Grzmil M., Huber R.M., Rutz-Schatzmann F., Kirchhoff F., Schaeren-Wiemers N., Gassmann M., et al. Constitutive Notch2 signaling in neural stem cells promotes tumorigenic features and astroglial lineage entry. Cell Death Dis. 2012;3:e325. doi: 10.1038/cddis.2012.65. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 104.Margareto J., Larrarte E., Leis O., Carrasco A., Lafuente J.V., Idoate M.A. Gene expression profiling of human gliomas reveals differences between GBM and LGA related to energy metabolism and notch signaling pathways. J. Mol. Neurosci. 2007;32:53–63. doi: 10.1007/s12031-007-0008-5. [DOI] [PubMed] [Google Scholar]
  • 105.Hai L., Zhang C., Li T., Zhou X., Liu B., Li S., Zhu M., Lin Y., Yu S., Zhang K., et al. Notch1 is a prognostic factor that is distinctly activated in the classical and proneural subtype of glioblastoma and that promotes glioma cell survival via the NF-κB(p65) pathway. Cell Death Dis. 2018;9:158. doi: 10.1038/s41419-017-0119-z. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 106.Cheng W., Zhang C., Ren X., Jiang Y., Han S., Liu Y., Cai J., Li M., Wang K., Liu Y., et al. Bioinformatic analyses reveal a distinct Notch activation induced by STAT3 phosphorylation in the mesenchymal subtype of glioblastoma. J. Neurosurg. 2017;126:249–259. doi: 10.3171/2015.11.JNS15432. [DOI] [PubMed] [Google Scholar]
  • 107.Verhaak R.G.W., Hoadley K.A., Purdom E., Wang V., Qi Y., Wilkerson M.D., Miller C.R., Ding L., Golub T., Mesirov J.P., et al. An integrated genomic analysis identifies clinically relevant subtypes of glioblastoma characterized by abnormalities in PDGFRA, IDH1, EGFR and NF1. Cancer Cell. 2010;17:98. doi: 10.1016/j.ccr.2009.12.020. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 108.Cooper L.A., Gutman D.A., Long Q., Johnson B.A., Cholleti S.R., Kurc T., Saltz J.H., Brat D.J., Moreno C.S. The proneural molecular signature is enriched in oligodendrogliomas and predicts improved survival among diffuse gliomas. PLoS ONE. 2010;5:e12548. doi: 10.1371/journal.pone.0012548. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 109.Ohgaki H., Kleihues P. The definition of primary and secondary glioblastoma. Clin. Cancer Res. 2013;19:764–772. doi: 10.1158/1078-0432.CCR-12-3002. [DOI] [PubMed] [Google Scholar]
  • 110.Spino M., Kurz S.C., Chiriboga L., Serrano J., Zeck B., Sen N., Patel S., Shen G., Vasudevaraja V., Tsirigos A., et al. Cell Surface Notch Ligand DLL3 is a Therapeutic Target in Isocitrate Dehydrogenase-mutant Glioma. Clin. Cancer Res. 2018 doi: 10.1158/1078-0432.CCR-18-2312. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 111.Jungk C., Mock A., Exner J., Geisenberger C., Warta R., Capper D., Abdollahi A., Friauf S., Lahrmann B., Grabe N., et al. Spatial transcriptome analysis reveals Notch pathway-associated prognostic markers in IDH1 wild-type glioblastoma involving the subventricular zone. BMC Med. 2016;14:170. doi: 10.1186/s12916-016-0710-7. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 112.Huber R.M., Rajski M., Sivasankaran B., Moncayo G., Hemmings B.A., Merlo A. Deltex-1 activates mitotic signaling and proliferation and increases the clonogenic and invasive potential of U373 and LN18 glioblastoma cells and correlates with patient survival. PLoS ONE. 2013;8:e57793. doi: 10.1371/journal.pone.0057793. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 113.Tsung A.J., Guda M.R., Asuthkar S., Labak C.M., Purvis I.J., Lu Y., Jain N., Bach S.E., Prasad D.V.R., Velpula K.K. Methylation regulates HEY1 expression in glioblastoma. Oncotarget. 2017;8:44398–44409. doi: 10.18632/oncotarget.17897. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 114.Sun P., Xia S., Lal B., Eberhart C.G., Quinones-Hinojosa A., Maciaczyk J., Matsui W., Dimeco F., Piccirillo S.M., Vescovi A.L., et al. DNER, an epigenetically modulated gene, regulates glioblastoma-derived neurosphere cell differentiation and tumor propagation. Stem Cells. 2009;27:1473–1486. doi: 10.1002/stem.89. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 115.Sun J., Gong X., Purow B., Zhao Z. Uncovering MicroRNA and Transcription Factor Mediated Regulatory Networks in Glioblastoma. PLoS Comput. Biol. 2012;8:e1002488. doi: 10.1371/journal.pcbi.1002488. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 116.Li Y., Guessous F., Zhang Y., DiPierro C., Kefas B., Johnson E., Marcinkiewicz L., Jiang J., Yang Y., Schmittgen T.D., et al. microRNA-34a inhibits glioblastoma growth by targeting multiple oncogenes. Cancer Res. 2009;69:7569–7576. doi: 10.1158/0008-5472.CAN-09-0529. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 117.Di Bari M., Bevilacqua V., De Jaco A., Laneve P., Piovesana R., Trobiani L., Talora C., Caffarelli E., Tata A.M. Mir-34a-5p Mediates Cross-Talk between M2 Muscarinic Receptors and Notch-1/EGFR Pathways in U87MG Glioblastoma Cells: Implication in Cell Proliferation. Int. J. Mol. Sci. 2018;19:1631. doi: 10.3390/ijms19061631. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 118.Wu Z., Wu Y., Tian Y., Sun X., Liu J., Ren H., Liang C., Song L., Hu H., Wang L., et al. Differential effects of miR-34c-3p and miR-34c-5p on the proliferation, apoptosis and invasion of glioma cells. Oncol. Lett. 2013;6:1447–1452. doi: 10.3892/ol.2013.1579. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 119.Ruan J., Lou S., Dai Q., Mao D., Ji J., Sun X. Tumor suppressor miR-181c attenuates proliferation, invasion, and self-renewal abilities in glioblastoma. Neuroreport. 2015;26:66–73. doi: 10.1097/WNR.0000000000000302. [DOI] [PubMed] [Google Scholar]
  • 120.Wong H.A., Fatimy R.E., Onodera C., Wei Z., Yi M., Mohan A., Gowrisankaran S., Karmali P., Marcusson E., Wakimoto H., et al. The Cancer Genome Atlas Analysis Predicts MicroRNA for Targeting Cancer Growth and Vascularization in Glioblastoma. Mol. Ther. 2015;23:1234–1247. doi: 10.1038/mt.2015.72. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 121.Turchi L., Debruyne D.N., Almairac F., Virolle V., Fareh M., Neirijnck Y., Burel-Vandenbos F., Paquis P., Junier M.P., Van Obberghen-Schilling E., et al. Tumorigenic potential of miR-18A* in glioma initiating cells requires NOTCH-1 signaling. Stem Cells. 2013;31:1252–1265. doi: 10.1002/stem.1373. [DOI] [PubMed] [Google Scholar]
  • 122.Wang H., Sun T., Hu J., Zhang R., Rao Y., Wang S., Chen R., McLendon R.E., Friedman A.H., Keir S.T., et al. miR-33a promotes glioma-initiating cell self-renewal via PKA and NOTCH pathways. J. Clin. Investig. 2014;124:4489–4502. doi: 10.1172/JCI75284. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 123.Moutal A., Honnorat J., Massoma P., Desormeaux P., Bertrand C., Malleval C., Watrin C., Chounlamountri N., Mayeur M.E., Besancon R., et al. CRMP5 Controls Glioblastoma Cell Proliferation and Survival through Notch-Dependent Signaling. Cancer Res. 2015;75:3519–3528. doi: 10.1158/0008-5472.CAN-14-0631. [DOI] [PubMed] [Google Scholar]
  • 124.Liu M., Inoue K., Leng T., Guo S., Xiong Z.G. TRPM7 channels regulate glioma stem cell through STAT3 and Notch signaling pathways. Cell Signal. 2014;26:2773–2781. doi: 10.1016/j.cellsig.2014.08.020. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 125.Chigurupati S., Venkataraman R., Barrera D., Naganathan A., Madan M., Paul L., Pattisapu J.V., Kyriazis G.A., Sugaya K., Bushnev S., et al. Receptor channel TRPC6 is a key mediator of Notch-driven glioblastoma growth and invasiveness. Cancer Res. 2010;70:418–427. doi: 10.1158/0008-5472.CAN-09-2654. [DOI] [PubMed] [Google Scholar]
  • 126.Kristoffersen K., Nedergaard M.K., Villingshoj M., Borup R., Broholm H., Kjaer A., Poulsen H.S., Stockhausen M.T. Inhibition of Notch signaling alters the phenotype of orthotopic tumors formed from glioblastoma multiforme neurosphere cells but does not hamper intracranial tumor growth regardless of endogene Notch pathway signature. Cancer Biol. Ther. 2014;15:862–877. doi: 10.4161/cbt.28876. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 127.Raghu H., Gondi C.S., Dinh D.H., Gujrati M., Rao J.S. Specific knockdown of uPA/uPAR attenuates invasion in glioblastoma cells and xenografts by inhibition of cleavage and trafficking of Notch -1 receptor. Mol. Cancer. 2011;10:130. doi: 10.1186/1476-4598-10-130. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 128.Wang X., Hu M., Xing F., Wang M., Wang B., Qian D. Human cytomegalovirus infection promotes the stemness of U251 glioma cells. J. Med. Virol. 2017;89:878–886. doi: 10.1002/jmv.24708. [DOI] [PubMed] [Google Scholar]
  • 129.Wang J., Wang C., Meng Q., Li S., Sun X., Bo Y., Yao W. siRNA targeting Notch-1 decreases glioma stem cell proliferation and tumor growth. Mol. Biol. Rep. 2012;39:2497–2503. doi: 10.1007/s11033-011-1001-1. [DOI] [PubMed] [Google Scholar]
  • 130.Sivasankaran B., Degen M., Ghaffari A., Hegi M.E., Hamou M.F., Ionescu M.C., Zweifel C., Tolnay M., Wasner M., Mergenthaler S., et al. Tenascin-C is a novel RBPJkappa-induced target gene for Notch signaling in gliomas. Cancer Res. 2009;69:458–465. doi: 10.1158/0008-5472.CAN-08-2610. [DOI] [PubMed] [Google Scholar]
  • 131.Li Y., He Z.C., Zhang X.N., Liu Q., Chen C., Zhu Z., Chen Q., Shi Y., Yao X.H., Cui Y.H., et al. Stanniocalcin-1 augments stem-like traits of glioblastoma cells through binding and activating NOTCH1. Cancer Lett. 2018;416:66–74. doi: 10.1016/j.canlet.2017.11.033. [DOI] [PubMed] [Google Scholar]
  • 132.Kim S.H., Kim E.J., Hitomi M., Oh S.Y., Jin X., Jeon H.M., Beck S., Jin X., Kim J.K., Park C.G., et al. The LIM-only transcription factor LMO2 determines tumorigenic and angiogenic traits in glioma stem cells. Cell Death Differ. 2015;22:1517–1525. doi: 10.1038/cdd.2015.7. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 133.Jeon H.M., Jin X., Lee J.S., Oh S.Y., Sohn Y.W., Park H.J., Joo K.M., Park W.Y., Nam D.H., DePinho R.A., et al. Inhibitor of differentiation 4 drives brain tumor-initiating cell genesis through cyclin E and notch signaling. Genes Dev. 2008;22:2028–2033. doi: 10.1101/gad.1668708. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 134.Chen G., Kong J., Tucker-Burden C., Anand M., Rong Y., Rahman F., Moreno C.S., Van Meir E.G., Hadjipanayis C.G., Brat D.J. Human Brat ortholog TRIM3 is a tumor suppressor that regulates asymmetric cell division in glioblastoma. Cancer Res. 2014;74:4536–4548. doi: 10.1158/0008-5472.CAN-13-3703. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 135.Zhu T.S., Costello M.A., Talsma C.E., Flack C.G., Crowley J.G., Hamm L.L., He X., Hervey-Jumper S.L., Heth J.A., Muraszko K.M., et al. Endothelial cells create a stem cell niche in glioblastoma by providing NOTCH ligands that nurture self-renewal of cancer stem-like cells. Cancer Res. 2011;71:6061–6072. doi: 10.1158/0008-5472.CAN-10-4269. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 136.Zhang C., Hai L., Zhu M., Yu S., Li T., Lin Y., Liu B., Zhou X., Chen L., Zhao P., et al. Actin cytoskeleton regulator Arp2/3 complex is required for DLL1 activating Notch1 signaling to maintain the stem cell phenotype of glioma initiating cells. Oncotarget. 2017;8:33353–33364. doi: 10.18632/oncotarget.16495. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 137.Kolosa K., Motaln H., Herold-Mende C., Korsic M., Lah T.T. Paracrine effects of mesenchymal stem cells induce senescence and differentiation of glioblastoma stem-like cells. Cell Transplant. 2015;24:631–644. doi: 10.3727/096368915X687787. [DOI] [PubMed] [Google Scholar]
  • 138.Irshad K., Mohapatra S.K., Srivastava C., Garg H., Mishra S., Dikshit B., Sarkar C., Gupta D., Chandra P.S., Chattopadhyay P., et al. A combined gene signature of hypoxia and notch pathway in human glioblastoma and its prognostic relevance. PLoS ONE. 2015;10:e0118201. doi: 10.1371/journal.pone.0118201. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 139.Bar E.E., Lin A., Mahairaki V., Matsui W., Eberhart C.G. Hypoxia increases the expression of stem-cell markers and promotes clonogenicity in glioblastoma neurospheres. Am. J. Pathol. 2010;177:1491–1502. doi: 10.2353/ajpath.2010.091021. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 140.Bayin N.S., Frenster J.D., Sen R., Si S., Modrek A.S., Galifianakis N., Dolgalev I., Ortenzi V., Illa-Bochaca I., Khahera A., et al. Notch signaling regulates metabolic heterogeneity in glioblastoma stem cells. Oncotarget. 2017;8:64932–64953. doi: 10.18632/oncotarget.18117. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 141.Charles N., Ozawa T., Squatrito M., Bleau A.M., Brennan C.W., Hambardzumyan D., Holland E.C. Perivascular nitric oxide activates notch signaling and promotes stem-like character in PDGF-induced glioma cells. Cell Stem Cell. 2010;6:141–152. doi: 10.1016/j.stem.2010.01.001. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 142.Jeon H.-M., Kim S.-H., Jin X., Park J.B., Kim S.H., Joshi K., Nakano I., Kim H. Crosstalk between Glioma-Initiating Cells and Endothelial Cells Drives Tumor Progression. Cancer Res. 2014;74:4482–4492. doi: 10.1158/0008-5472.CAN-13-1597. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 143.Jubb A.M., Browning L., Campo L., Turley H., Steers G., Thurston G., Harris A.L., Ansorge O. Expression of vascular Notch ligands Delta-like 4 and Jagged-1 in glioblastoma. Histopathology. 2012;60:740–747. doi: 10.1111/j.1365-2559.2011.04138.x. [DOI] [PubMed] [Google Scholar]
  • 144.Rampazzo E., Persano L., Pistollato F., Moro E., Frasson C., Porazzi P., Della Puppa A., Bresolin S., Battilana G., Indraccolo S., et al. Wnt activation promotes neuronal differentiation of glioblastoma. Cell Death Dis. 2013;4:e500. doi: 10.1038/cddis.2013.32. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 145.Dali R., Verginelli F., Pramatarova A., Sladek R., Stifani S. Characterization of a FOXG1:TLE1 transcriptional network in glioblastoma initiating cells. Mol. Oncol. 2018;12:775–787. doi: 10.1002/1878-0261.12168. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 146.Hovinga K.E., Shimizu F., Wang R., Panagiotakos G., Van Der Heijden M., Moayedpardazi H., Correia A.S., Soulet D., Major T., Menon J., et al. Inhibition of notch signaling in glioblastoma targets cancer stem cells via an endothelial cell intermediate. Stem Cells. 2010;28:1019–1029. doi: 10.1002/stem.429. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 147.Kristoffersen K., Villingshoj M., Poulsen H.S., Stockhausen M.T. Level of Notch activation determines the effect on growth and stem cell-like features in glioblastoma multiforme neurosphere cultures. Cancer Biol. Ther. 2013;14:625–637. doi: 10.4161/cbt.24595. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 148.Staberg M., Michaelsen S.R., Olsen L.S., Nedergaard M.K., Villingshoj M., Stockhausen M.T., Hamerlik P., Poulsen H.S. Combined EGFR- and notch inhibition display additive inhibitory effect on glioblastoma cell viability and glioblastoma-induced endothelial cell sprouting in vitro. Cancer Cell Int. 2016;16:34. doi: 10.1186/s12935-016-0309-2. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 149.Monticone M., Biollo E., Fabiano A., Fabbi M., Daga A., Romeo F., Maffei M., Melotti A., Giaretti W., Corte G., et al. z-Leucinyl-leucinyl-norleucinal induces apoptosis of human glioblastoma tumor-initiating cells by proteasome inhibition and mitotic arrest response. Mol. Cancer Res. 2009;7:1822–1834. doi: 10.1158/1541-7786.MCR-09-0225. [DOI] [PubMed] [Google Scholar]
  • 150.Tanaka S., Nakada M., Yamada D., Nakano I., Todo T., Ino Y., Hoshii T., Tadokoro Y., Ohta K., Ali M.A., et al. Strong therapeutic potential of gamma-secretase inhibitor MRK003 for CD44-high and CD133-low glioblastoma initiating cells. J. Neurooncol. 2015;121:239–250. doi: 10.1007/s11060-014-1630-z. [DOI] [PubMed] [Google Scholar]
  • 151.Saito N., Fu J., Zheng S., Yao J., Wang S., Liu D.D., Yuan Y., Sulman E.P., Lang F.F., Colman H., et al. A high Notch pathway activation predicts response to gamma secretase inhibitors in proneural subtype of glioma tumor-initiating cells. Stem Cells. 2014;32:301–312. doi: 10.1002/stem.1528. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 152.Yahyanejad S., King H., Iglesias V.S., Granton P.V., Barbeau L.M., van Hoof S.J., Groot A.J., Habets R., Prickaerts J., Chalmers A.J., et al. NOTCH blockade combined with radiation therapy and temozolomide prolongs survival of orthotopic glioblastoma. Oncotarget. 2016;7:41251–41264. doi: 10.18632/oncotarget.9275. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 153.Chen J., Kesari S., Rooney C., Strack P.R., Chen J., Shen H., Wu L., Griffin J.D. Inhibition of notch signaling blocks growth of glioblastoma cell lines and tumor neurospheres. Genes Cancer. 2010;1:822–835. doi: 10.1177/1947601910383564. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 154.Kahlert U.D., Cheng M., Koch K., Marchionni L., Fan X., Raabe E.H., Maciaczyk J., Glunde K., Eberhart C.G. Alterations in cellular metabolome after pharmacological inhibition of Notch in glioblastoma cells. Int. J. Cancer. 2016;138:1246–1255. doi: 10.1002/ijc.29873. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 155.Fan X., Khaki L., Zhu T.S., Soules M.E., Talsma C.E., Gul N., Koh C., Zhang J., Li Y.M., Maciaczyk J., et al. NOTCH pathway blockade depletes CD133-positive glioblastoma cells and inhibits growth of tumor neurospheres and xenografts. Stem Cells. 2010;28:5–16. doi: 10.1002/stem.254. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 156.Cenciarelli C., Marei H.E., Zonfrillo M., Pierimarchi P., Paldino E., Casalbore P., Felsani A., Vescovi A.L., Maira G., Mangiola A. PDGF receptor alpha inhibition induces apoptosis in glioblastoma cancer stem cells refractory to anti-Notch and anti-EGFR treatment. Mol. Cancer. 2014;13:247. doi: 10.1186/1476-4598-13-247. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 157.Wu Y., Cain-Hom C., Choy L., Hagenbeek T.J., de Leon G.P., Chen Y., Finkle D., Venook R., Wu X., Ridgway J., et al. Therapeutic antibody targeting of individual Notch receptors. Nature. 2010;464:1052–1057. doi: 10.1038/nature08878. [DOI] [PubMed] [Google Scholar]
  • 158.Floyd D.H., Kefas B., Seleverstov O., Mykhaylyk O., Dominguez C., Comeau L., Plank C., Purow B. Alpha-secretase inhibition reduces human glioblastoma stem cell growth in vitro and in vivo by inhibiting Notch. Neuro Oncol. 2012;14:1215–1226. doi: 10.1093/neuonc/nos157. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 159.Faibish M., Francescone R., Bentley B., Yan W., Shao R. A YKL-40-neutralizing antibody blocks tumor angiogenesis and progression: A potential therapeutic agent in cancers. Mol. Cancer Ther. 2011;10:742–751. doi: 10.1158/1535-7163.MCT-10-0868. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 160.Alimoghaddam K. A review of arsenic trioxide and acute promyelocytic leukemia. Int. J. Hematol. Oncol. Stem Cell Res. 2014;8:44–54. [PMC free article] [PubMed] [Google Scholar]
  • 161.Zhen Y., Zhao S., Li Q., Li Y., Kawamoto K. Arsenic trioxide-mediated Notch pathway inhibition depletes the cancer stem-like cell population in gliomas. Cancer Lett. 2010;292:64–72. doi: 10.1016/j.canlet.2009.11.005. [DOI] [PubMed] [Google Scholar]
  • 162.Wu J., Ji Z., Liu H., Liu Y., Han D., Shi C., Shi C., Wang C., Yang G., Chen X., et al. Arsenic trioxide depletes cancer stem-like cells and inhibits repopulation of neurosphere derived from glioblastoma by downregulation of Notch pathway. Toxicol. Lett. 2013;220:61–69. doi: 10.1016/j.toxlet.2013.03.019. [DOI] [PubMed] [Google Scholar]
  • 163.Ding D., Lim K.S., Eberhart C.G. Arsenic trioxide inhibits Hedgehog, Notch and stem cell properties in glioblastoma neurospheres. Acta Neuropathol. Commun. 2014;2:31. doi: 10.1186/2051-5960-2-31. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 164.Ma Y., Cheng Z., Liu J., Torre-Healy L., Lathia J.D., Nakano I., Guo Y., Thompson R.C., Freeman M.L., Wang J. Inhibition of Farnesyltransferase Potentiates NOTCH-Targeted Therapy against Glioblastoma Stem Cells. Stem Cell Rep. 2017;9:1948–1960. doi: 10.1016/j.stemcr.2017.10.028. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 165.Chang K.H., Yan M.D., Yao C.J., Lin P.C., Lai G.M. Honokiol-induced apoptosis and autophagy in glioblastoma multiforme cells. Oncol. Lett. 2013;6:1435–1438. doi: 10.3892/ol.2013.1548. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 166.Lai I.C., Shih P.H., Yao C.J., Yeh C.T., Wang-Peng J., Lui T.N., Chuang S.E., Hu T.S., Lai T.Y., Lai G.M. Elimination of cancer stem-like cells and potentiation of temozolomide sensitivity by Honokiol in glioblastoma multiforme cells. PLoS ONE. 2015;10:e0114830. doi: 10.1371/journal.pone.0114830. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 167.Nandhu M.S., Behera P., Bhaskaran V., Longo S.L., Barrera-Arenas L.M., Sengupta S., Rodriguez-Gil D.J., Chiocca E.A., Viapiano M.S. Development of a Function-Blocking Antibody Against Fibulin-3 as a Targeted Reagent for Glioblastoma. Clin. Cancer Res. 2018;24:821–833. doi: 10.1158/1078-0432.CCR-17-1628. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 168.Li Y., Hu Y., Liu C., Wang Q., Han X., Han Y., Xie X.S., Chen X.H., Li X., Siegel E.R., et al. Human fibulin-3 protein variant expresses anti-cancer effects in the malignant glioma extracellular compartment in intracranial xenograft models. Oncotarget. 2017;8:106311–106323. doi: 10.18632/oncotarget.22344. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 169.Opacak-Bernardi T., Ryu J.S., Raucher D. Effects of cell penetrating Notch inhibitory peptide conjugated to elastin-like polypeptide on glioblastoma cells. J. Drug Target. 2017;25:523–531. doi: 10.1080/1061186X.2017.1289537. [DOI] [PubMed] [Google Scholar]
  • 170.Ying M., Wang S., Sang Y., Sun P., Lal B., Goodwin C.R., Guerrero-Cazares H., Quinones-Hinojosa A., Laterra J., Xia S. Regulation of glioblastoma stem cells by retinoic acid: Role for Notch pathway inhibition. Oncogene. 2011;30:3454–3467. doi: 10.1038/onc.2011.58. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 171.Phillips E., Lang V., Bohlen J., Bethke F., Puccio L., Tichy D., Herold-Mende C., Hielscher T., Lichter P., Goidts V. Targeting atypical protein kinase C iota reduces viability in glioblastoma stem-like cells via a notch signaling mechanism. Int. J. Cancer. 2016;139:1776–1787. doi: 10.1002/ijc.30234. [DOI] [PubMed] [Google Scholar]
  • 172.Xie Q., Wu Q., Kim L., Miller T.E., Liau B.B., Mack S.C., Yang K., Factor D.C., Fang X., Huang Z., et al. RBPJ maintains brain tumor–initiating cells through CDK9-mediated transcriptional elongation. J. Clin. Investig. 2016;126:2757–2772. doi: 10.1172/JCI86114. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 173.Schreck K.C., Taylor P., Marchionni L., Gopalakrishnan V., Bar E.E., Gaiano N., Eberhart C.G. The Notch target Hes1 directly modulates Gli1 expression and Hedgehog signaling: A potential mechanism of therapeutic resistance. Clin. Cancer Res. 2010;16:6060–6070. doi: 10.1158/1078-0432.CCR-10-1624. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 174.Maciaczyk D., Picard D., Zhao L., Koch K., Herrera-Rios D., Li G., Marquardt V., Pauck D., Hoerbelt T., Zhang W., et al. CBF1 is clinically prognostic and serves as a target to block cellular invasion and chemoresistance of EMT-like glioblastoma cells. Br. J. Cancer. 2017;117:102–112. doi: 10.1038/bjc.2017.157. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 175.Wang J., Wakeman T.P., Lathia J.D., Hjelmeland A.B., Wang X.F., White R.R., Rich J.N., Sullenger B.A. Notch promotes radioresistance of glioma stem cells. Stem Cells. 2010;28:17–28. doi: 10.1002/stem.261. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 176.Liu G., Yuan X., Zeng Z., Tunici P., Ng H., Abdulkadir I.R., Lu L., Irvin D., Black K.L., Yu J.S. Analysis of gene expression and chemoresistance of CD133+ cancer stem cells in glioblastoma. Mol. Cancer. 2006;5:67. doi: 10.1186/1476-4598-5-67. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 177.Park N.I., Guilhamon P., Desai K., McAdam R.F., Langille E., O’Connor M., Lan X., Whetstone H., Coutinho F.J., Vanner R.J., et al. ASCL1 Reorganizes Chromatin to Direct Neuronal Fate and Suppress Tumorigenicity of Glioblastoma Stem Cells. Cell Stem Cell. 2017;21:209–224.e7. doi: 10.1016/j.stem.2017.06.004. [DOI] [PubMed] [Google Scholar]
  • 178.Natsumeda M., Maitani K., Liu Y., Miyahara H., Kaur H., Chu Q., Zhang H., Kahlert U.D., Eberhart C.G. Targeting Notch Signaling and Autophagy Increases Cytotoxicity in Glioblastoma Neurospheres. Brain Pathol. 2016;26:713–723. doi: 10.1111/bpa.12343. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 179.Weber D., Lehal R., Frismantas V., Bourquin J., Bauer M., Murone M., Radtke F. Pharmacological Activity of CB-103 in Haematological Malignancies—An Oral Pan-Notch Inhibitor with a Novel Mode of Actcion. Hematol. Oncol. 2017;35:46. doi: 10.1002/hon.2437_28. [DOI] [Google Scholar]
  • 180.Strosberg J.R., Yeatman T., Weber J., Coppola D., Schell M.J., Han G., Almhanna K., Kim R., Valone T., Jump H., et al. A phase II study of RO4929097 in metastatic colorectal cancer. Eur. J. Cancer. 2012;48:997–1003. doi: 10.1016/j.ejca.2012.02.056. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 181.Xu R., Shimizu F., Hovinga K., Beal K., Karimi S., Droms L., Peck K.K., Gutin P., Iorgulescu J.B., Kaley T., et al. Molecular and Clinical Effects of Notch Inhibition in Glioma Patients: A Phase 0/I Trial. Clin. Cancer Res. 2016;22:4786–4796. doi: 10.1158/1078-0432.CCR-16-0048. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 182.Pan E., Supko J.G., Kaley T.J., Butowski N.A., Cloughesy T., Jung J., Desideri S., Grossman S., Ye X., Park D.M. Phase I study of RO4929097 with bevacizumab in patients with recurrent malignant glioma. J. Neurooncol. 2016;130:571–579. doi: 10.1007/s11060-016-2263-1. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Cancers are provided here courtesy of Multidisciplinary Digital Publishing Institute (MDPI)

RESOURCES