History and progress of hypotheses and clinical trials for Alzheimer’s disease

Abstract

Alzheimer’s disease (AD) is a neurodegenerative disease characterized by progressive memory loss along with neuropsychiatric symptoms and a decline in activities of daily life. Its main pathological features are cerebral atrophy, amyloid plaques, and neurofibrillary tangles in the brains of patients. There are various descriptive hypotheses regarding the causes of AD, including the cholinergic hypothesis, amyloid hypothesis, tau propagation hypothesis, mitochondrial cascade hypothesis, calcium homeostasis hypothesis, neurovascular hypothesis, inflammatory hypothesis, metal ion hypothesis, and lymphatic system hypothesis. However, the ultimate etiology of AD remains obscure. In this review, we discuss the main hypotheses of AD and related clinical trials. Wealthy puzzles and lessons have made it possible to develop explanatory theories and identify potential strategies for therapeutic interventions for AD. The combination of hypometabolism and autophagy deficiency is likely to be a causative factor for AD. We further propose that fluoxetine, a selective serotonin reuptake inhibitor, has the potential to treat AD.

Introduction

Alzheimer’s disease (AD) is an irreversible progressive neurological disorder that is characterized by memory loss, the retardation of thinking and reasoning, and changes in personality and behaviors.^1,2 AD seriously endangers the physical and mental health of the elderly. Aging is the biggest risk factor for the disease, the incidence of which doubles every 5 years after the age of 65.³ Approximately 40 million people over the age of 60 worldwide suffer from AD, and the number of patients is increasing, doubling every 20 years.^4–7

In 1906, Alois Alzheimer presented his first signature case and the pathological features of the disease at the 37th convention of Southwestern German Psychiatrists. Later, in 1910, his coworker Emil Kraepelin named the disease in honor of his achievements. In the following years (from 1910 to 1963), researchers and physicians did not pay much attention to the disease until Robert Terry and Michael Kidd revived interest by performing electron microscopy of neuropathological lesions in 1963. Electron microscopy analysis showed that neurofibrillary tangles (NFTs) were present in brain biopsies from two patients with advanced AD.^8,9 Since then, studies on the pathological features and mechanisms of AD and drug treatments for the disease have been conducted for more than half a century (from 1963 to present).¹⁰

Clinically, AD is divided into sporadic AD (SAD) and familial AD (FD). FD accounts for 1–5% of all AD cases.^11–15 In the early 1990s, linkage analyses of early-onset FD determined that mutations in three genes, namely, amyloid-beta A4 precursor protein (APP), presenilin 1 (PSEN1), and presenilin 2 (PSEN2), are involved in FD. PSEN1 mutations account for ~81% of FD cases, APP accounts for ~14%, and PSEN2 accounts for ~6%.¹¹ In addition to these three genes (APP, PSEN1, and PSEN2), more than 20 genetic risk loci for AD have been identified.^16,17 The strongest genetic risk factor for AD is the ε4 allele of apolipoprotein E (APOE).^18–21 APOE is a class of proteins involved in lipid metabolism and is immunochemically colocalized to senile plaques, vascular amyloid deposits, and NFTs in AD. The APOE gene is located on chromosome 19q13.2 and is associated with late-onset FD. The APOE gene has three alleles, namely, ε2, ε3, and ε4, with frequencies of 8.4%, 77.9%, and 13.7%, respectively. The differences in APOE2 (Cys112, Cys158), APOE3 (Cys112, Arg158), and APOE4 (Arg112, Arg158) are limited to amino acid residues 112 and 158.^22–25 Analyses of the frequencies of these APOE alleles among human populations have revealed that there is a significant association between APOE4 and late-onset FD (with an ε4 allele frequency of ~40% in AD), suggesting that ApoE4 may be an important susceptibility factor for the etiopathology of AD.^25–27 Moreover, APOE4 can increase the neurotoxicity of β-amyloid (Aβ) and promote filament formation.²⁸ The APOE4 genotype influences the timing and amount of amyloid deposition in the human brain.²⁹ Reelin signaling protects synapses against toxic Aβ through APOE receptors, which suggests that APOE is a potential target for AD therapy.³⁰

The incidence of SAD accounts for more than 95% of all AD cases. Therefore, in this review, we focus our attention on recent SAD research and clinical trials. There are various descriptive hypotheses regarding the causes of SAD, including the cholinergic hypothesis,³¹ amyloid hypothesis,^32,33 tau propagation hypothesis,³⁴ mitochondrial cascade hypothesis,³⁵ calcium homeostasis hypothesis,³⁶ inflammatory hypothesis,³⁷ neurovascular hypothesis,³⁸ metal ion hypothesis,³⁹ and lymphatic system hypothesis.⁴⁰ In addition, there are many other factors that increase the risk for SAD, including family history,⁴¹ midlife hypertension,⁴² sleep disorders,⁴³ midlife obesity,⁴⁴ and oxidative stress.^45,46 Interestingly, according to the latest evaluation of single-nucleotide polymorphisms (SNPs), Mukherjee et al. found 33 SNPs associated with AD and assigned people to six cognitively defined subgroups.⁴⁷

At present, clinical drug treatments are mainly divided into two categories: acetylcholinesterase inhibitors (AChEIs), represented by donepezil, and the antagonist of N-methyl-D-aspartic acid (NMDA) receptor, represented by memantine (Table 1).⁴⁸ As neurotransmitter regulators, these drugs can only relieve symptoms for a short time but cannot delay the progression of AD. Recent failures and the limited progress of therapeutics in phase III clinical trials suggest that it is time to consider alternative strategies for AD treatment.⁴⁹

Table 1.

Summary of pharmacological parameters of AD drugs

Drug	Time (approved by FDA)	Chemical class	Action	Type of inhibition	Route of administration	Indication	Status
Tacrine	1995	Alkaline	AChE inhibitor	Rapidly reversible	Oral or rectal	_	Withdrawal
Donepezil	1996	Piperidine	AChE inhibitor	Rapidly reversible	Oral	Mild- moderately (mod) AD	Approved
Rivastigmine	1997	Carbamate	AChE and BChE inhibitor	Pseudoreversible	Oral or transdermal patch	Mild-mod AD	Approved
Galantamine	2001	Phenanthrene alkaloid	AChE inhibitor	Rapidly reversible	Oral	Mild-mod AD	Approved
Memantine	2003	Glutamatergic modulator	NMDA antagonist	N/A	Oral	Mod-severe AD	Approved

In this review, we discuss the hypotheses of the molecular mechanisms of AD and related clinical trials (Fig. 1) and hope that these discussions will be helpful for developing explanatory theories and potential effective strategies for AD treatment.

Fig. 1.

Percentage of clinical trials in which each of the various hypotheses for AD were tested up to 2019. The amyloid hypothesis was the most heavily tested (22.3% of trials); the neurotransmitter hypothesis was the second most tested (19.0% of trials); the percentage of trials that tested the tau propagation hypothesis was ~12.7%; 17.0% of trials tested the mitochondrial cascade hypothesis and related hypotheses; 7.9% of trials tested the neurovascular hypothesis; 6.6% of trials tested the exercise hypothesis; 4.6% of trials tested the inflammatory hypothesis; 0.5% of trials tested the virus hypothesis; and the other uncatalogued trials made up approximately 8.4% of all trials

Hypotheses

Cholinergic hypothesis

The cholinergic hypothesis was proposed by Peter Davies and A. J. F. Maloney in 1976³¹. They studied and compared the activities of the key enzymes involved in the synthesis of neurotransmitters, including acetylcholine, γ-aminobutyric acid, dopamine, noradrenaline, and 5-hydroxytryptamine, in 20 regions of AD and control brains. The activity of choline acetyltransferase in the AD brains was greatly reduced in the amygdala, hippocampus, and cortex, in which the concentration of acetylcholine was decreased at synapses.^50–52 The activity of glutamic acid decarboxylase, tyrosine hydroxylase, aromatic amino acid decarboxylase, dopamine-β-hydroxylase, and monoamine oxidase in all the areas of the AD brains studied appeared to be well within the normal range. Choline acetyltransferase is a key enzyme in the synthesis of acetylcholine, and its catalytic activity requires these substrates: choline, acetyl-CoA, and adenosine triphosphate (ATP). This was the first time that the concept of AD was noted as a cholinergic system failure.^31,53 This finding has also been reported in other neurological and psychiatric disorders, such as Parkinson’s disease (PD) and depression.^54,55

AChEIs can alleviate cognitive impairment in AD patients by inhibiting the degradation of acetylcholine.^56–59 Therefore, AChEIs have been used for more than 20 years since the FDA approved tacrine, the first drug for the treatment of AD, in 1995.⁶⁰ Tacrine is a reversible AChEI. Because of its liver toxicity, the number of tacrine prescriptions dropped after other AChEIs were introduced, and the usage of tacrine has been largely discontinued. The second generations of AChEI drugs that are widely used at present include donepezil, rivastigmine, and galantamine.^61,62 These drugs show fewer side effects and higher central selectivity and improve the cognition level of patients with mild to moderate AD. The daily living ability and overall function of patients treated with rivastigmine and galantamine are better than those treated with donepezil.^61–63 According to the latest meta-analysis on the efficacy of AChEIs for treating the cognitive symptoms of dementia, AChEIs have modest effects on dementia in AD,⁶⁴ but the effect is not continuous.^65,66

In conclusion, the current clinical drugs used for the treatment of AD improve the quality of life of AD patients, but have no significant effect on the occurrence or progression of AD. In 2012, the French Pharmacoeconomic Committee assessed the medical benefit of these drugs and downgraded its rating of the medical benefit provided by AChEIs in AD from "major" to "low."⁶⁷

Amyloid hypothesis

The amyloid hypothesis was first proposed in 1991 by John Hardy and David Allsop.^32,33 They found a pathogenic mutation in the Aβ precursor protein (APP) gene on chromosome 21, which suggested that APP mismetabolism and Aβ deposition were the primary events in AD. They thought that the pathological cascades in AD were Aβ deposition, tau phosphorylation, NFT formation, and neuronal death. The presence of Aβ deposits in an APP mutant (APP751) transgenic model supported the hypothesis and further contributed to shifting the amyloid hypothesis from a descriptive to a mechanistic hypothesis.^68,69 Positron emission tomography (PET) imaging studies have suggested that ~30% of clinically normal older individuals have signs of Aβ accumulation.^70–73

Aβ was first isolated by Glenner and Wong in 1984.⁷⁴ Aβ may provide a strategy for diagnostic testing for AD and for understanding its pathogenesis.⁷⁴ APP was first cloned and sequenced in 1987; APP consists of 695 amino acid residues and a glycosylated receptor located on the cell surface.^75,76 Aβ is composed of 39–43 residues derived from multiple proteolytic cleavages of APP. APP is cleaved in two ways (Fig. 2). The first method is through the α pathway. APP is hydrolyzed by α-secretase and then by γ-secretase; this process does not produce insoluble Aβ. The second method is through the β pathway. APP is hydrolyzed by β-secretase (BACE1) and then by γ-secretase to produce insoluble Aβ. Under normal conditions, the Aβ protein is not produced since APP hydrolysis is mainly based on the α pathway. A small amount of APP is hydrolyzed via the second method, and the Aβ that produced is eliminated by the immune system. However, when some mutations, such as the Lys670Asn/Met671Leu (Swedish) and Ala673Val mutations near the BACE1 cleavage site, are present,^77,78 APP is prone to hydrolysis by the β pathway, resulting in an excessive accumulation of insoluble Aβ and eventually the development of AD.^79,80 However, the Ala673Thr mutation has been suggested to be protective.⁸¹

Fig. 2.

Schematic of the amyloid hypothesis and tau hypothesis. Upper: The transmembrane APP protein can be cleaved by two pathways. Under normal processing, APP is hydrolyzed by α-secretase and then by γ-secretase, which does not produce insoluble Aβ; under abnormal processing, APP is hydrolyzed by β secretase (BACE1) and then by γ secretase, which produces insoluble Aβ. Phase III clinical trials of solanezumab (Eli Lilly), crenezumab (Roche/Genentech/AC Immune), aducanumab (Biogen Idec), and umibecestat (Novartis/Amgen), which target the amyloid hypothesis, have all been terminated thus far. Lower: The tau protein can be hyperphosphorylated at amino residues Ser202, Thr205, Ser396, and Ser404 (which are responsible for tubulin binding), thereby leading to the release of tau from microtubules and the destabilization of microtubules. Hyperphosphorylated tau monomers aggregate to form complex oligomers and eventually neurofibrillary tangles, which may cause cell death

High concentrations of Aβ protein are neurotoxic to mature neurons because they cause dendritic and axonal atrophy followed by neuronal death.⁸² The levels of insoluble Aβ are correlated with the decline of cognition.⁸³ In addition, Aβ inhibits hippocampal long-term potentiation (LTP) in vivo.⁸⁴ Neurofibrillary degeneration is enhanced in tau and APP mutant transgenic mice.⁸⁵ Transgenic mice that highly express human APP in the brain exhibit spontaneous seizures, which may be due to enhanced synaptic GABAergic inhibition and deficits in synaptic plasticity.⁸⁶ Individuals with Aβ are prone to cognitive decline^87–89 and symptomatic AD phenotypes.^90,91

The current strategies for AD treatment based on the Aβ hypothesis are mainly divided into the following categories: β- and γ-secretase inhibitors, which are used to inhibit Aβ production; antiaggregation drugs (including metal chelators), which are used to inhibit Aβ aggregation; protease activity-regulating drugs, which are used to clear Aβ; and immunotherapy.⁹² We will discuss recent progress regarding immunotherapy and BACE1 inhibitors.

Aβ-targeting monoclonal antibodies (mAbs) are the major passive immunotherapy treatments for AD. For example, solanezumab (Eli Lilly), which can bind monomeric and soluble Aβ, failed to show curative effects in AD patients in phase III, although solanezumab effectively reduced free plasma Aβ concentrations by more than 90%.⁹³ Gantenerumab (Roche/Genentech) is a mAb that binds oligomeric and fibrillar Aβ and can activate the microglia-mediated phagocytic clearance of plaques. However, it also failed in phase III.⁹⁴ Crenezumab (Roche/Genentech/AC Immune) is a mAb that can bind to various Aβ, including monomers, oligomers, and fibrils. On January 30, 2019, Roche announced the termination of two phase III trials of crenezumab in AD patients. Aducanumab (Biogen Idec) is a mAb that targets aggregated forms of Aβ. Although aducanumab can significantly reduce Aβ deposition, Biogen and Eisai announced the discontinuation of trials of aducanumab on March 21, 2019. Together, the failure of these trials strongly suggests that it is better to treat Aβ deposits as a pathological feature rather than as part of a major mechanistic hypothesis.

BACE1 inhibitors aim to reduce Aβ and have been tested for years. However, no BACE1 inhibitors have passed clinical trials. Verubecestat (MK-8931, Merck & Co.) reduced Aβ levels by up to 90% in the cerebrospinal fluid (CSF) in AD. However, Merck no longer listed verubecestat in its research pipeline since verubecestat did not improve cognitive decline in AD patients and was associated with unfavorable side effects.⁹⁵ Lanabecestat (AZD3293, AstraZeneca/Eli Lilly) is another BACE1 inhibitor that can lower CSF Aβ levels by up to 75%. However, on June 12, 2018, phase II/III trials of lanabecestat were discontinued due to a lack of efficacy. The BACE1 inhibitor atabecestat (JNJ-54861911, Janssen) induced a robust reduction in Aβ levels by up to 95% in a phase I trial. However, Janssen announced the discontinuation of this program on May 17, 2018. The latest news regarding the BACE inhibitor umibecestat (Novartis/Amgen) was released on July 11, 2019; it was announced that the evaluation of umibecestat was discontinued in phase II/III trials since an assessment demonstrated a worsening of cognitive function. Elenbecestat (E2609, Eisai) is another BACE1 inhibitor that can reduce CSF Aβ levels by up to 80%^96,97 and is now in phase III trials (shown in Table 2). Although all BACE1 inhibitors seem to reduce CSF Aβ levels, the failure of trials of solanezumab, which can reduce free plasma Aβ concentrations by more than 90%,⁹³ may be sufficient to lead us to pessimistic expectations, especially considering that the treatment worsened cognition and induced side effects.

Table 2.

Current status of selected AD drugs in clinical trials

Drug	Developer	Mechanism of action	Stage	NCT number (https://clinicaltrials.gov)
Gantenerumab (RO4909832)	Roche/Genentech	Aβ-specific mAb	Phase III	NCT03443973
Solanezumab (LY2062430)	Eli Lilly	Aβ-specific mAb	Phase III	NCT02760602
Aducanumab (BIIB037)	Biogen Inc.	Aβ-specific mAb	Phase III (terminated)	NCT02484547
Crenezumab	Roche/AC Immune	Aβ-specific mAb	Phase III (terminated)	NCT02670083
AAB-003 (PF‑05236812)	Janssen/Pfizer	Aβ-specific mAb	Phase I (terminated)457	NCT01193608
Donanemab (N3pG‑Aβ)	Eli Lilly	Aβ-specific mAb	Phase II	NCT03367403
MEDI1814	AstraZeneca	Aβ-specific mAb	Phase I	NCT02036645
CAD106	Novartis	Aβ vaccine	Phase II	NCT01097096
ACI-24	AC Immune	Aβ vaccine	Phase I	NCT02738450
Verubecestat (MK-8931)	Merck & Co.	BACE1 inhibitor	Phase III (terminated)	NCT01953601
Lanabecestat (AZD3293)	AstraZeneca/Eli Lilly	BACE1 inhibitor	Phase III (terminated)	NCT02783573
Elenbecestat (E2609)	Eisai	BACE1 inhibitor	Phase III	NCT02036280
Atabecestat (JNJ-54861911)	Janssen	BACE1 inhibitor	Phase III (terminated)	NCT02569398
Umibecestat (CNP520)	Novartis/Amgen	BACE1 inhibitor	Phase II/III (terminated)	NCT03131453
LMTM (TRx0237)	TauRx Therapeutics	Tau-aggregation inhibitor	Phase III	NCT01626378
ACI-35	AC Immune/Janssen	Tau vaccine	Phase I	ISRCTN13033912
AADvac1	Axon Neuroscience SE	Tau vaccine	Phase II	NCT02579252
Naproxen	Seattle Institute for Biomedical and Clinical Research	Anti-inflammatory medication	Phase III (terminated)	NCT00007189
Celecoxib	Seattle Institute for Biomedical and Clinical Research	Anti-inflammatory medication	Phase III (terminated)	NCT00007189
Simvastatin	Charite University, Berlin, Germany	Cholesterol-lowering drug	Phase IV	NCT00842920
Valaciclovir	New York State Psychiatric Institute	Antiviral drug	Phase II	NCT03282916
Gemfibrozil	Gregory Jicha	MicroRNA-107 expression regulator	Early phase I	NCT02045056
Rosiglitazone	GlaxoSmithKline	Type II diabetes drug	Phase III (terminated)	NCT00490568
Neflamapimod (VX-745)	EIP Pharma, LLC	p38a kinase inhibitor	Phase II	NCT02423122
Ketasyn (AC-1202)	University of California, Los Angeles	Ketone body elevator	Phase IV	NCT01122329
GV-971	Shanghai Green Valley Pharmaceutical Co., Ltd.	Mannose oligosaccharide diacid	Phase III	NCT02293915

Tau propagation hypothesis

Intracellular tau-containing NFTs are an important pathological feature of AD.^98,99 NFTs are mainly formed by the aggregation of paired helical filaments (Fig. 2). Pathological NFTs are mainly composed of tau proteins, which are hyperphosphorylated.^100–103 Tau proteins belong to a family of microtubule-binding proteins, and are heterogeneous in molecular weight. A main function of tau is to stabilize microtubules, which is particularly important for neurons since microtubules serve as highways for transporting cargo in dendrites and axons.^34,104 Tau cDNA, which encodes a protein of 352 residues, was cloned and sequenced in 1988. RNA blot analysis has identified two major transcripts that are 6 and 2 kilobases long and are widely distributed in the brain.^105,106 The alternative splicing of exons 2, 3, and 10 of the tau gene produces six tau isoforms in humans; the differential splicing of exon 10 leads to tau species that contain various microtubule-binding carboxyl terminals with repeats of three arginines (3R) or four arginines (4R).^107,108 An equimolar ratio of 3R and 4R may be important for preventing tau from forming aggregates.¹⁰⁹

The tau propagation hypothesis was introduced in 2009.³⁴ The pathology of tau usually first appears in discrete and specific areas and later spreads to more regions of the brain. Aggregates of fibrillar and misfolded tau may propagate in a prion-like way through cells, eventually spreading through the brains of AD patients (Fig. 2). Clavaguera et al. demonstrated that tau can act as an endopathogen in vivo and in culture studies in vitro with a tau fragment.¹⁰⁴ In their study, brain extracts isolated from P301S tau transgenic mice¹¹⁰ were injected into the brains (the hippocampus and cortical areas) of young ALZ17 mice, a tau transgenic mouse line that only develops late tau pathology.¹¹¹ After the injection, the ALZ17 mice developed tau pathology quickly, whereas the brain extracts from wild-type mice or immunodepleted P301S mice, which were used as controls, had no effect. The causes of tau aggregation in sporadic tauopathies are not fully understood. Tau can be phosphorylated at multiple serine and threonine residues (Fig. 2).^112,113 The gain- and loss-of-function of tau phosphorylation may be due to alterations in the activities of kinases or phosphatases that target tau, and thus, the toxicity of tau can be augmented as a result. Other posttranslational modifications can decrease tau phosphorylation or enhance the harmful states of tau. For example, serine–threonine modifications by O-glycosylation can reduce the extent of tau phosphorylation.^114,115 Thus, tau hyperphosphorylation may partially result from a decrease in tau O-glycosylation. In addition, tau can also be phosphorylated at tyrosine residues,¹¹⁶ sumoylated and nitrated,¹¹⁷ but the exact roles of these tau modifications remain elusive.

According to the tau propagation hypothesis, abnormally phosphorylated tau proteins depolymerize microtubules and affect signal transmission within and between neurons.^101,103,118 In addition, mutant forms of human tau cause enhanced neurotoxicity in Drosophila melanogaster.¹¹⁹ There may be cross-talk between the tau propagation hypothesis and the amyloid hypothesis. As mentioned earlier, among the risk loci for AD, APOE is the most robust factor for AD pathogenesis.¹²⁰ Unlike other isoforms, APOE4 may increase Aβ by decreasing its clearance^121–123 and enhancing tau hyperphosphorylation.^124–126 GSK3 is one of the upstream factors that jointly regulates Aβ and tau. Increased GSK3 activity leads to the hyperphosphorylation of the tau protein.¹²⁶ GSK3 overactivity may also affect the enzymatic processing of APP and thus increase the Aβ level.^127,128 In addition, tau is essential for Aβ-induced neurotoxicity, and dendritic tau can mediate Aβ-induced synaptic toxicity and circuit abnormalities.¹²⁹ Moreover, APP and tau act together to regulate iron homeostasis. APP can interact with ferroportin-1 to regulate the efflux of ferrous ions.^130,131 As an intracellular microtubule-associated protein, tau can increase iron output by enhancing the transport of APP to the cell surface.¹³² Decreased APP trafficking to the cell surface accounts for iron accumulation in tau knockout neurons.^133,134

As one of the most important hypotheses of AD, the tau propagation hypothesis has a wide range of impacts. Drugs that target the tau protein are divided into the following categories: tau assembly inhibitors, tau kinase inhibitors, O-GlcNAcase inhibitors, microtubule stabilizers, and immunotherapy drugs.⁹² Only a few agents have undergone proof-of-principle tests as tau kinase inhibitors, microtubule-stabilizing agents, and inhibitors of heat shock protein 90 (Hsp90), which stabilize GSK3β.^135,136 In addition, some inhibitors of tau aggregation, such as TRx0237 (TauRx Therapeutics), are in clinical trials. The results of TRx 237–005 phase III clinical trials showed that the agent may be effective as a monotherapy since the brain atrophy rate of AD patients declined after 9 months of treatment.¹³⁷ ACI-35 (AC Immune/Janssen) and AADvac1 (Axon Neuroscience SE) are vaccines that target the hyperphosphorylated tau protein, and the vaccines are still being evaluated in clinical trials¹³⁸ (Table 2). Tau-directed therapies will inevitably face challenges similar to those presently encountered in Aβ-targeted trials. Overall, the effectiveness of tau-directed therapies remains to be tested in the future.

Mitochondrial cascade hypothesis and related hypotheses (Fig. 3)

Fig. 3.

Mitochondrial cascade and related hypotheses. Mitochondria are the main contributors to ROS production, which is significantly increased in AD. The metabolites of mitochondrial TCA, such as pyruvate, fumarate, malate, OAA, and α-KG, not only directly regulate energy production but also play an important role in the epigenetic regulation of neurons and longevity.^{164,173,187–189} For example, SAM provides methyl groups for histone and DNA methyltransferases (HMTs and DNMTs).^165,166 α-KG is a necessary cofactor for TET DNA methylases, histone demethylases (HDMs), and lysine demethylases KDMs/JMJDs.^167,168 Mitochondria also regulate the levels and redox state of FAD, a cofactor of the histone demethylase LSD1.¹⁷⁵ Dysfunctional mitochondria can be removed by mitophagy, which is also very important in the progression of AD. BNIP3L interacts with LC3 or GABARAP and regulates the recruitment of damaged mitochondria to phagophores. In addition, Beclin 1 is released from its interaction with Bcl-2 to activate autophagy after BNIP3L competes with it. PINK1 promotes autophagy by recruiting the E3 ligase PARK2. Then, VDAC1 is ubiquitinated and then binds to SQSTM1. SQSTM1 can interact with LC3 and target this complex to the autophagosome.⁴⁴⁵ L. monocytogenes can promote the aggregation of NLRX1 and the binding of LC3, thus activating mitophagy.⁴⁴⁶ The MARCH5-FUNDC1 axis mediates hypoxia-induced mitophagy.⁴⁴⁷ The mitochondrial proteins NIPSNAP1 and NIPSNAP2 can recruit autophagy receptors and bind to autophagy-related proteins.⁴⁴⁸ ROS: reactive oxygen species; TCA: tricarboxylic acid cycle; OAA: oxaloacetate; α-KG: α-ketoglutarate; SAM: S-adenosyl methionine; TET: ten–eleven translocation methylcytosine dioxygenase; FAD: flavin adenine dinucleotide

In 2004, Swerdlow and Khan first introduced the mitochondrial cascade hypothesis³⁵ and stated that mitochondrial function may affect the expression and processing of APP and the accumulation of Aβ in SAD. The hypothesis includes three main parts. First, an individual's baseline mitochondrial function is defined by genetic inheritance. Second, the rate of age-associated mitochondrial changes is determined by inherited and environmental factors. Moreover, a decline in mitochondrial function or efficiency drives aging phenotypes.^139–141 Third, the rate of change of mitochondrial function in individuals influences AD chronology.

Oxidative stress is defined as “an imbalance in pro-oxidants and antioxidants with associated disruption of redox circuitry and macromolecular damage.”¹⁴² Oxidative stress is mainly caused by increased levels of reactive oxygen species (ROS) and/or reactive nitrogen species, including superoxide radical anions (O²⁻), hydrogen peroxide (H₂O₂), hydroxyl radicals (HO⁻), nitric oxide (NO), and peroxynitrite (ONOO⁻). In intact cells, ROS can be produced from multiple sources, including mitochondria, ER, peroxisomes, NADPH oxidases, and monoamine oxidases.^143,144 In AD, neurons exhibit significantly increased oxidative damage and a reduced number of mitochondria,¹⁴⁵ which are the main contributors to ROS generation among these ROS sources.^146,147 The overproduction of ROS and/or an insufficient antioxidant defense can lead to oxidative stress.¹⁴⁸ Before the onset of the clinical symptoms of AD and the appearance of Aβ pathology, there is evidence that the production of ROS increases due to mitochondrial damage.¹⁴⁸ Both mtDNA and cytochrome oxidase levels increase in AD, and the number of intact mitochondria is significantly reduced in AD.¹⁴⁵ Several key enzymes involved in oxidative metabolism, including dehydrogenase complexes for α-ketoglutarate (α-KG) and pyruvate, and cytochrome oxidase also show reduced expression or activity in AD.^149–154 In addition, there is evidence in vitro and in vivo for a direct relationship between oxidative stress and neuronal dysfunction in AD.^155,156 Aβ-dependent endocytosis is involved in reducing the number of NMDA receptors on the cell surface and synaptic plasticity in neurons and brain tissue in AD mice.¹⁵⁷ Excessive Aβ may also trigger excitotoxicity and stress-related signaling pathways by increasing Ca²⁺ influx, increasing oxidative stress, and impairing energy metabolism.¹⁵⁸

Although the majority of efforts have been focused on genetic variations and their roles in disease etiology, it has been postulated that epigenetic dysfunction may also be involved in AD.^159,160 Indeed, there is growing evidence that epigenetic dysregulation is linked to AD.^161–163 Mitochondrial metabolites are required for epigenetic modifications, such as the methylation of DNA and the methylation and acetylation of histones.¹⁶⁴ AD brains exhibited a global reduction in DNA modifications, including 5-methylcytosine and 5-hydroxymethylcytosine.^165–168 S-adenosyl methionine (SAM) provides a methyl group for histones and DNA methyltransferases in the nucleus. SAM is generated and maintained by coupling one-carbon metabolism and mitochondrial energy metabolism.^169,170 α-KG, which is generated by the tricarboxylic acid cycle (TCA) cycle in mitochondria and the cytosol, is a cofactor of ten–eleven translocation methylcytosine dioxygenase DNA methylases, histone demethylases (HDMs) and the lysine demethylases KDMs/JMJDs.^171,172 However, the activities of KDMs/JMJDs and TETs can be inhibited by fumarate, succinate, and 2-hydroxyglutarate.¹⁷³ Mutations that affect the succinate dehydrogenase complex and fumarate hydratase can induce the accumulation of succinate and fumarate, respectively.¹⁷⁴ Oxidized flavin adenine dinucleotide (FAD) is an essential cofactor of the HDM LSD1, a member of the KDM family.¹⁷⁵ In addition, acetyl-CoA, the source of acetyl groups that are consumed by histone acetyltransferases, is generated by ATP citrate lyase and pyruvate dehydrogenase in the cytosol and mitochondria, respectively.¹⁷⁶ In addition, oxidized nicotinamide adenine dinucleotide (NAD⁺) is a cofactor for sirtuins (SIRTs), a family of deacetylases that includes nuclear-localized SIRT1, SIRT6, and SIRT7, cytosolic SIRT2, and three mitochondrial SIRTs (SIRT3, SIRT4, and SIRT5) (Fig. 3). Therefore, the activities of SIRTs are sensitive and are regulated by cellular NAD⁺ pools.¹⁷⁷ As summarized by Fang, NAD⁺ replenishment can enhance autophagy/mitophagy mainly through SIRT1 or SIRT3; meanwhile, SIRT6 and SIRT7 induce autophagy through the inhibition of mTOR; NAD⁺ may also inhibit autophagy/mitophagy through SIRT2, SIRT4, SIRT5, and poly(ADP-ribose) polymerases.¹⁷⁸ In short, mitochondrial dysfunction can partially explain the epigenetic dysregulation in aging and AD.

Dysfunctional mitochondria can be removed by mitophagy, a term that was first coined by Dr Lemasters in 2005.¹⁷⁹ Since then, mitophagy has been linked to various diseases, including neurodegenerative disorders such as PD¹⁸⁰ and Huntington's disease (HD),¹⁸¹ as well as normal physiological aging.¹⁸² Mitophagosomes can effectively degrade their internalized cargo by fusing with lysosomes during axonal retrotransport.¹⁸³ Fang et al. demonstrated that neuronal mitophagy is impaired in AD.¹⁸⁴ Mitophagy stimulation can reverse memory impairment, diminish insoluble Aβ 1–42 and Aβ 1–40 through the microglial phagocytosis of extracellular Aβ plaques, and abolish AD-related tau hyperphosphorylation.¹⁸⁴ Therefore, deficiencies in mitophagy may have a pivotal role in AD etiology and may be a potential therapeutic target.^{178,184–186}

The metabolites of mitochondrial TCA, such as pyruvate, fumarate, malate, oxaloacetate (OAA), and α-KG, have been demonstrated to extend lifespan when fed to C. elegans.^{173,187–189} Wilkins et al. found that OAA enhances the energy metabolism of neuronal cells.¹⁹⁰ Moreover, OAA can also activate mitochondrial biogenesis in the brain, reduce inflammation, and stimulate neurogenesis.¹⁹¹ The application of OAA in AD was also investigated by Swerdlow et al., and the results showed that 100-mg OAA capsules did not result in an elevation of OAA in the blood¹⁹²; higher doses up to 2 g per day were also evaluated in clinical studies, but no results have been posted or published yet.

Clinical trials related to the mitochondrial cascade hypothesis and related hypotheses account for 17.0% of all clinical trials (Fig. 1). Based on the above, the mitochondrial cascade hypothesis and related hypotheses (Fig. 3) may link other hypotheses, including the cholinergic hypothesis, amyloid hypothesis, and tau propagation hypothesis.

Calcium homeostasis and NMDA hypotheses

The calcium homeostasis hypothesis was proposed in 1992 by Mattson et al. They found that Aβ can elevate intracellular calcium levels and render neurons more vulnerable to environmental stimuli.³⁶ The involvement of calcium in AD was first suggested long ago by Khachaturian,¹⁹³ and since then, there are many efforts to clarify this hypothesis.^194–196 Calcineurin can trigger reactive/inflammatory processes in astrocytes, which are upregulated in AD models.¹⁹⁷ In addition, calcium homeostasis is closely related to learning and memory. Rapid autopsies of the postmortem human brain have suggested that calcineurin/nuclear factor of activated T-cells signaling is selectively altered in AD and is involved in driving Aβ-mediated cognitive decline.¹⁹⁸ The evidence indicates that calcium homeostasis may be associated with the development of AD.^193,199

Memantine, a noncompetitive antagonist of NMDA glutamate receptors in the brain was approved for marketing in Europe in 2002 and received US FDA approval in 2003.^200,201 Memantine is not an AChEI. The functional mechanism of memantine likely involves blocking current flow (especial calcium currents) through NMDA receptors and reducing the excitotoxic effects of glutamate.²⁰² Memantine is also an antagonist of type 3 serotonergic (5-HT₃) receptors and nicotinic acetylcholine receptors, but it does not bind other receptors, such as adrenergic, dopamine, and GABA receptors. The inhibition of NMDA receptors can also reduce the inhibition of α-secretase and thus inhibit the production of Aβ.²⁰³ However, the French Pharmacoeconomic Committee downgraded its rating of the medical benefit provided by memantine in AD from "major" to "low,"⁶⁷ which was also supported by a recent meta-analysis.⁶⁴

Neurovascular hypothesis

The homeostasis of the microenvironment and metabolism in the brain relies on substrate delivery and the drainage of waste through the blood; neurons, astrocytes, and vascular cells form a delicate functional unit that supports the integrity of brain structure and function.^204–206 Vascular dysregulation leads to brain dysfunction and disease. Alterations in cerebrovascular function are features of both cerebrovascular pathologies and neurodegenerative diseases, including AD.³⁸ In 1994, it was demonstrated that the cerebral microvasculature is damaged in AD.²⁰⁷ Aβ can induce the constriction of the cerebral arteries.²⁰⁸ In an AD mouse model, neocortical microcirculation is impaired before Aβ accumulation.^209,210 Neuroimaging studies in AD patients have demonstrated that neurovascular dysfunction is found before the onset of neurodegeneration.^211–214 In addition to aberrant angiogenesis and the senescence of the cerebrovascular system, the faulty clearance of Aβ across the blood–brain barrier (BBB) can initiate neurovascular uncoupling and vessel regression and consequently cause brain hypoperfusion, brain hypoxia, and neurovascular inflammation. Eventually, BBB compromise and a chemical imbalance in the neuronal environment lead to neuronal dysfunction and loss.²¹⁵ In mice that overexpress APP, impairment in the neocortical microcirculation is observed. The cerebrovascular effects of Aβ in dementia may involve alterations in cerebral blood flow and neuronal dysfunction.²⁰⁹ Moreover, neurovascular dysfunction may also play a role in the etiology of AD.

Many factors can lead to changes in the neurovasculature, which in turn affect the occurrence and progression of AD. Of these factors, hyperlipidemia is one of the most important. During the last two decades, growing evidence has shown that a high cholesterol level may increase the risk of AD. In one test, higher levels of low-density lipoprotein (LDL) or total cholesterol were correlated with lower scores on the MMSE (modified mini mental state exam) in nondemented patients. High total cholesterol levels in midlife increase the risk of AD nearly threefold: the odds ratio (OR) is 2.8 (95% confidence interval, CI: 1.2–6.7).²¹⁶ Midlife obesity is also a risk factor for AD,²¹⁷ and midlife adiposity may predict an earlier onset of dementia and Aβ accumulation.²¹⁸ Adipose tissue secretes some inflammation factors, such as tumor necrosis factor (TNF-α), interleukin-1 (IL-1), and interleukin-6, in obesity,²¹⁹ and these factors may induce insulin resistance, produce Aβ deposits, and stimulate excessive tau phosphorylation.²²⁰

A hyperglycemic state is another risk factor. Type 2 diabetic patients (T2D) have an increased risk of dementia,²²¹ both vascular dementia (VD) and AD. In the largest and latest meta-analysis of T2D and dementia risk, data from 6184 individuals with diabetes and 38,350 without diabetes were pooled and analyzed.²²² The relative risk (RR) for dementia was 1.51 (95%CI: 1.31–1.74). The results of the analyses further suggested that there are two common subtypes of dementia: AD and VD. The results suggested that T2D conferred an RR of 2.48 (95%CI: 2.08–2.96) for VD and 1.46 (95%CI: 1.20–1.77) for AD.²²² Insulin resistance is a common feature of T2D and SAD. Accumulating evidence supports the involvement of impaired insulin signaling in AD progression. Insulin levels and insulin receptor expression are reduced in AD brains.²²³ However, plasma insulin and Aβ levels are both increased in AD patients, suggesting that a decrease in insulin clearance may increase plasma Aβ levels. Blocking insulin signaling in the brain through the intracerebroventricular administration of STZ (the diabetogenic drug streptozotocin) resulted in various pathological features that resemble those found in human SAD, while the administration of insulin and glucose enhances learning and memory in AD patients.^224,225

Many institutions have conducted clinical trials of statins, drugs that are used to lower blood cholesterol, for the treatment of AD. However, in a phase IV clinical trial, simvastatin failed to reduce Aβ-42 and tau levels in the CSF. The results suggested that the use of statins for the treatment of AD requires more evidence.²²⁶ To test the hyperglycemic hypothesis, rosiglitazone (RSG), a drug used for the treatment for type II diabetes mellitus, was evaluated. RSG XR had no effect in a phase III trial.²²⁷ In addition, hypertension has also been linked to worse cognition and hypometabolism in AD. AD patients with hypertension exhibit worse cognitive function (on the AD assessment scale-cognitive subscale, P = 0.038) and a higher burden of neuropsychiatric symptoms (on the neuropsychiatric inventory questionnaire, P = 0.016) than those without hypertension.²²⁸ As an antihypertensive medication, ramipril is a specific angiotensin-converting enzyme inhibitor; however, ramipril was tested and failed in a pilot clinical trial.²²⁹

Therefore, trial failures of treatments related to the neurovascular hypothesis and related hypotheses suggest that these hypotheses alone may not be sufficient to explain the etiology of AD.

Inflammatory hypothesis

The inflammatory responses of microglia and astrocytes in the central nervous system (CNS) also play important roles in the development of AD.^230–232 Microglial cells are brain-specific macrophages in the CNS, and they make up 10–15% all brain cells.²³³ Microglia cells exhibit higher activity in AD patients than in the control group.²³⁴ The concentration of aggregated microglial cells near senile plaques and neurons with NFTs in AD patients is usually 2–5 times higher than that in normal individuals. Inflammatory factors that are expressed by microglia and histocompatibility complexes also cause inflammation.²³⁵ In vitro studies have linked Aβ pathology in AD to neuroinflammation. It has been shown that Aβ possesses a synergistic effect on the cytokine-induced activation of microglia.²³⁶ Two studies have confirmed that Aβ can induce glial activation in vivo.^237,238 The fibrillar conformation of Aβ seems to be crucial for such activation.²³⁹ In AD patients, Aβ can bind to microglia cells through the CD36-TLR4-TLR6 receptor complex and the NLRP3 inflammatory complex, destroy cells, release inflammation-inducing factors, such as TNF-α, and cause immune responses. In addition to increased levels of TNF-α, increased levels of the inflammatory cytokines IL-1β, TGF-β, IL-12, and IL-18 in the CNS are also correlated with AD progression and increase damage in the brains of AD patients.²⁴⁰ Interestingly, CD22 is a B-cell receptor that functions as a negative regulator of phagocytosis. The functional decline of aged microglia may result from the upregulation of CD22; thus, the inhibition of CD22 can enhance the clearance of debris and fibrils, including Aβ oligomers, in vivo, and this process may be potentially beneficial for the treatment of AD.²⁴¹

Considerable evidence suggests that the use of anti-inflammatory drugs may be linked with a reduced occurrence of AD. The ability of naproxen and celecoxib to delay or prevent the onset of AD and cognitive decline was evaluated in phase III clinical trials. However, therapeutic efficacy analysis indicated that naproxen and celecoxib do not exert a greater benefit compared with that of placebo. In addition, the naproxen and celecoxib groups experienced more adverse events, including hypertension, gastrointestinal, and vascular or cardiac problems, so these phase III clinical trials were discontinued.²⁴² A clinical trial of lornoxicam in AD patients was also terminated due to a lack of efficacy. These failures suggest that the clinical application of anti-inflammatory drugs for AD treatment needs to be further validated (Table 2).

Metal ion hypothesis

Metal ions that play functional roles in organisms are classified as biometals, while other metal ions are inert or toxic.^243,244 The dyshomeostasis of any metal ion in the body usually leads to disease. In the CNS, biometals, such as copper, zinc, and iron, are required to act as cofactors for enzymatic activity, mitochondrial function, and neuronal function.^245,246 In healthy brains, free metal ions are stringently regulated and kept at a very low level.²⁴⁷

Biometal ions are involved in Aβ aggregation and toxicity. In the first study that evaluated biometals and Aβ, which was published by Bush et al. in 1994, zinc was linked to Aβ. The potential link between biometals and AD has been intensively studied.^39,248–250 There is evidence of the dyshomeostasis of biometals in AD brains. Biometals, especially zinc and copper, are directly coordinated by Aβ, and biometals such as iron can reach a high concentration (~1 mM) in plaques.^251,252 In the serum, the levels of copper, which are not associated with ceruloplasmin, are elevated in AD patients. Moreover, a higher copper content in the serum is associated with lower MMSE scores.^253,254 In the serum of AD patients, the levels of Zn²⁺ ions are decreased compared with those in age-matched controls, whereas the concentration of Zn²⁺ is elevated in the CSF.²⁵⁵

The important role of biometals in Aβ formation has been reported in various animal models. For example, the role of Cu²⁺ in Aβ formation was demonstrated in a cholesterol-fed rabbit model of AD.²⁵⁶ Administering trace amounts of Cu²⁺ in drinking water was sufficient to induce Aβ accumulation, the consequent formation of plaques, and deficits in learning.²⁵⁶ On the other hand, Cu²⁺ also plays a beneficial role. For example, transgenic mice that overexpress mutant human APP and are treated with Cu²⁺ show a reduction in Aβ and do not exhibit a lethal phenotype.²⁵⁷ In contrast, in Drosophila that specifically express human Aβ in the eye, dietary zinc and copper increase Aβ-associated damage, while different chelators of biometals demonstrate favorable effects.²⁵⁸

During normal aging, the gradual accumulation of iron is observed in some brain areas, such as the substantia nigra, putamen, globus pallidus, and caudate nucleus.^259–263 An increase in the level of iron in AD brains was first demonstrated in 1953.²⁶⁴ More recently, through the use of magnetic resonance imaging (MRI), iron accumulation was found in AD and was shown to be mainly localized to certain brain areas, such as the parietal cortex, motor cortex, and hippocampus.^265–272 Studies of gene mutations that affect the metabolism of iron have suggested that the dyshomeostasis of iron plays a role in neuronal death, such as the neuronal death that occurs in neurodegenerative disorders such as AD.^273–277 Iron overload accelerates neuronal Aβ production and consequently worsens cognitive decline in a transgenic AD mice.²⁷⁸ There is evidence that the levels of labile iron can directly affect APP production via iron regulatory element.²⁷⁹ As a potent source of highly toxic hydroxyl radicals, redox-active iron is actively associated with senile plaques and NFTs.²⁸⁰

As the most common nutrient deficiency in the world, iron deficiency is also frequently observed and reported in AD.²⁸¹ Iron is present in polynuclear iron–sulfur (Fe/S) centers and hemoproteins. Mitochondrial complexes I–III require Fe/S clusters, and complexes II–IV need hemoproteins for electron transfer and the oxidative phosphorylation of the respiratory chain.²⁸² Thus, iron deficiency may partially account for hypometabolism in AD since women with iron deficiency anemia have a higher prevalence of dementia.²⁸³ Interestingly, iron deficiency and iron accumulation in AD seem paradoxical. One potential explanation is that tau differentially regulates the motor proteins dynein and kinesin; specifically, tau may preferentially inhibit kinesin, which transports cargo toward the cell periphery.²⁸⁴ Tau is distributed in a proximal-to-distal gradient with a low concentration in the cell body.^284–287 When tau is hyperphosphorylated, it is released from the distal microtubules into the neuronal axon and soma, and thus inhibits kinesin activity and prevents the transport of iron-containing cargo and other cargo (including mitochondria) to the neuronal periphery; this may result in the accumulation of mtDNA and iron accumulations in the soma of neurons in AD^145,280 and deficiencies in mitochondria and iron homeostasis in the white matter of the brain. Iron-targeted therapies were recently updated and reviewed.²⁸⁸ Similar to the amyloid hypothesis, the conjecture that the therapeutic chelation of iron ions is an effective approach for treating AD remains widespread despite a lack of evidence of any clinical benefits.²⁸⁸

Aluminum (Al), the most abundant metal in the earth’s crust, is a nonessential metal ion in organisms. The role of Al in AD needs to be further elucidated. Exley et al. hypothesized that Al is associated with Aβ in AD brains and Al can precipitate Aβ in vitro into fibrillar structures; in addition, Al is known to increase the Aβ burden in the brains of treated animals, which may be due to a direct or indirect effect on Aβ anabolism and catabolism.^289,290

Biometals may play various roles in AD and may influence the pathogenesis directly or indirectly. For example, biometals indirectly influence energy metabolism and APP processing,²⁴⁹ while cellular iron levels can directly regulate APP through IREs identified in the 5′ -UTR of mRNA.^291,292

Lymphatic system hypothesis

The lymphatic network and the blood vasculature are essential for fluid balance in the body.^293,294 Below the human skull, the meninges, a three-layer membrane that envelopes the brain, contains a network of lymphatic vessels. This meningeal lymphatic system was first discovered in 1787, and interest in this system has been revived recently.^295–297 Proteins, metabolites, and waste produced by the brain flow through the interstitial fluid (ISF) and reach the CSF, which circulates through the ventricles and brain meninges.²⁹⁸ In the classical form of transvascular removal, metabolic waste and other molecules in these fluids are drained from the brain, are transported across capillary walls, and cross the BBB.^298,299 Thrane et al.’s found that, in addition to transvascular removal, perivascular removal, in which the blood vasculature allows the CSF to flow into or exit the brain along the para-arterial space or via paravenous routes, occurs and that aquaporin-4 water channels that are expressed in astrocytes are essential for CSF–ISF exchange along the perivascular pathway.^300,301 This perivascular route is called the glymphatic system.^302,303

During aging, impairments in the transvascular/perivascular removal of waste may result in Aβ accumulation in the brain.^40,304 Animals that lack aquaporin-4 channels show a 70% decrease in the ability to remove large solutes, such as Aβ.^305,306 Da Masquita et al.’s investigated the importance of meningeal lymphatics for Aβ production in AD mouse models. They found that ablating meningeal lymphatics leads to Aβ accumulation in the meninges, accelerates Aβ deposition, and induces cognitive deficits. These findings are consistent with Aβ accumulation observed in the meninges of AD patients. Strategies for promoting the growth of meningeal lymphatic vessels may have the potential to enhance the clearance of Aβ and lessen the deposition of Aβ,^307,308 but this remains to be further validated.

Other hypotheses

In addition to the above hypotheses, there are many other factors that can affect the occurrence of AD. For a long time (at least 60 years), investigators have suspected that microbes may be involved in the onset and progression of AD, this was hypothesized by Sjogren et al. beginning in 1952.³⁰⁹ In addition to McLachlan et al.’s proposal in 1980,³¹⁰ several investigators have proposed that AD may be caused by a viral form of herpes simplex.^311–314 There have been intensive reports suggesting that AD may be associated with various bacterial and viral pathogens,^315–317 especially herpesviridae (including HSV-1,^318,319 EBV, HCMV, HHV-6A, and HHV-7^314,320). However, these studies did not determine the underlying mechanisms or identify a robust association with a specific viral species. Recent reports have suggested that Aβ aggregation and deposition may be stimulated by different classes of microbes as a part of the innate immune response. Microbes trigger amyloidosis, and newly generated Aβ acts as an antimicrobial peptide to coat microbial particles to fight the infection.^321–323 Valaciclovir, an antiviral drug that is used for the management of herpes simplex and herpes zoster, is now in a phase II trial for AD (Table 2).

MicroRNAs (miRNAs) are involved in posttranscriptional gene regulation.^324–327 The decreased expression of miRNA-107 (miR-107) in AD may accelerate disease progression by regulating the expression of BACE1.³²⁸ In SAD patients, the expression of miR-29a/b-1 is inversely correlated with BACE1 expression.³²⁹ Only one clinical trial related to miRNAs is underway. Gregory Jicha launched a phase I trial to assess the safety and efficacy of gemfibrozil in modulating miR-107 levels for the prevention of AD in subjects with cognitive impairment (Table 2).

Mannose oligosaccharide diacid (GV-971) was developed by researchers at the Shanghai Institute of Medicine, the Chinese Academy of Sciences, the Ocean University of China, and the Shanghai Green Valley Pharmaceutical Co., Ltd. GV-971 is an oceanic oligosaccharide molecule extracted from seaweed. GV-971 may capture multiple fragments of Aβ in multiple sites and multiple states, inhibit the formation of Aβ filaments, and depolymerize filaments into nontoxic monomers^330,331; however, an understanding of the exact mechanism is still lacking. GV-971 has been reported to improve learning and memory in Aβ-treated mice.³³² In phase II trials, GV-971 improved cognition in AD patients.³³³ In addition, a phase III clinical trial of GV-971 finished with positive results, and it is on its way to the market in China (Table 2).

Interestingly, a pilot clinical trial that included 120 nondemented elderly Chinese individuals (ages 60–79) living in Shanghai compared the effects of interventions (such as walking, Tai Chi, and social interaction) on cognition and whole brain volume, as determined by a neuropsychological battery and MRI scans.³³⁴ The results showed that Tai Chi and social interaction were beneficial, but walking had no effect. Therefore, in addition to promising drugs, a healthy lifestyle can delay the progression of AD.

Opinions

The whole brain atrophy rate is −0.67 to −0.8% per year in adulthood.³³⁵ Freeman et al.’s results demonstrated that, although the frontal and temporal regions of the cortex undergoing thinning, the total number of neurons remains relatively constant from age 56 to age 103. However, there is a reduction in the number of hippocampal neurons in AD but not in normal aging. The loss of neuronal structural complexity may contribute to the thinning that occurs with aging.³³⁶ The integrity of neurons and dendritic structures is the basis for maintaining the normal function of neurons.^337–339 Brain atrophy affects the function of neurons, which in turn impairs signal transmission and causes movement disorders, cognitive disorders etc.^340–343 Brain atrophy has been shown to be a key pathological change in AD.^344–347 In particular, the annual atrophy rate of the hippocampus in AD patients (−3.98 ± 1.92%) is two to four times that of the atrophy rate in healthy individuals (−1.55 ± 1.38%). At the same time, the annual increase in the temporal lobe volume of the lateral ventricle in AD patients (14.16 ± 8.47%) is significantly greater than that in healthy individuals (6.15 ± 7.69%).³⁴⁸ The ratio of the volume of the lateral ventricle to the volume of the hippocampus may be a reliable measurement for evaluating AD since the ratio can minimize variances and fluctuations in clinical data and may be a more objective and sensitive method for diagnosis and evaluating AD. In 1975, brain atrophy and a reduction in perfusion were detected in AD patients.³⁴⁹ In 1980, atrophy of hippocampal neurons and abnormal brain metabolism were first discovered in AD patients with PET.³⁵⁰ Brain volume reduction in patients with AD is significantly associated with dementia severity and cognitive disturbances as well as neuropsychiatric symptoms.³⁵¹ The development of broad-spectrum drugs that target brain atrophy, a common feature of neurodegenerative diseases, is still ongoing. In our previous work, RAS–RAF–MEK signaling was demonstrated to protect hippocampal neurons from atrophy caused by dynein dysfunction and mitochondrial hypometabolism (tetramethylrhodamine ethyl ester mediated mitochondrial inhibition), suggesting the feasibility of interventions for neuronal atrophy.³⁵²

The MAPK pathway protects neurons against dendritic atrophy and relies on MEK-dependent autophagy.³⁵² Autophagy is the principal cellular pathway by which degraded proteins and organelles are recycled, and it plays an essential role in cell fate in response to stress.^353–357 Aged organelles and protein aggregates are cleared by the autophagosome–lysosome pathway, which is particularly important in neurons.^358–360 Growing evidence has implicated defective autophagy in neurodegenerative diseases, including AD, PD, amyotrophic lateral sclerosis and HD.^{358,361–364} Recent work using live-cell imaging determined that autophagosomes preferentially form at the axon tip and undergo retrograde transport to the cell body.³⁶⁵ As a key protein in autophagy, Beclin 1 is decreased in the early stage of AD.^357,366,367 Moreover, a decrease in autophagy induced by the genetic ablation of Beclin 1 increases intracellular Aβ accumulation, extracellular Aβ deposition, and neurodegeneration.³⁶⁸ Autophagy decline also causes microglial impairments and neuronal ultrastructural abnormalities.³⁶⁸ On the other hand, transcriptome evidence has revealed enhanced autophagy–lysosome function in centenarians.³⁶⁹ PPARA-mediated autophagy can reduce AD-like pathology and cognitive decline.³⁷⁰ These results suggest that autophagy is a potential therapeutic target for AD. MEK-dependent autophagy is protective in neuronal cells.³⁵² The activation of the MEK–ERK signaling pathway can reduce the production of toxic amyloid Aβ by inhibiting γ-secretase activity.^371–375 Thus, MEK-dependent autophagy may provide a potential way to enhance Aβ and NFT clearance and may also be a new potential target for AD therapy (Fig. 4).

Fig. 4.

Schematic representation of autophagy. Yellow box: mTOR-dependent autophagy pathways. Growth factors can inhibit autophagy via activating the PI3K/Akt/mTORC1 pathway; under nutrient-rich conditions, mTORC1 is activated, whereas under starvation and oxidative stress, mTORC1 is inhibited. AMPK-dependent autophagy activation can be induced by starvation and hypoxia.⁴⁴⁹ Ras can also activate autophagy via activating PI3K,³⁵² while p300 can inhibit autophagy.⁴⁵⁰ p38 promotes autophagy by phosphorylating and inactivating Rheb and then inhibiting mTOR under stress.⁴⁵¹ Green boxes: mTOR-independent autophagy pathways. The PI3KCIII complex (also called the Beclin 1–Vps34–Vps15 complex) is essential for the induction of autophagy and is regulated by interacting proteins, such as the negative regulators Rubicon, Mcl-1, and Bcl-XL/Bcl-2, while proteins including UVRAG, Atg14, Bif-1, VMP-1, and Ambra-1 induce autophagy by binding Beclin 1 and Vps34 and promoting the activity of the PI3KCIII complex.³⁵⁷ In addition, various kinases also regulate autophagy. ERK and JNK-1 can phosphorylate Bcl-2, release its inhibition, and consequently induce autophagy; the phosphorylation of Beclin 1 by Akt inhibits autophagy, whereas the phosphorylation of Beclin 1 by DAPK promotes autophagy.⁴⁵² Autophagy can be inhibited by the action of PKA and PKC on LC3. Finally, Atg4, Atg3, Atg7, and Atg10 are autophagy-related proteins that mediate the formation of the Atg12–Atg5–Atg16L1 complex and LC3-II.⁴⁵³ RAS and p300 can also regulate autophagy via the mTOR-independent pathway⁴⁵⁴

Hypometabolism is sufficient to cause neuronal atrophy in vitro and in vivo.^352,376,377 Hypometabolism may be a potential therapeutic target for AD.³⁷⁸ Regional hypometabolism is another characteristic of AD brains (Fig. 5). The human brain makes up ~2% of the body weight but consumes up to ~20% of the oxygen supply; the brain is energy demanding and relies on the efficiency of the mitochondrial TCA cycle and oxidative phosphorylation for ATP generation.^379–382 However, glucose metabolism in the brain in AD and mild cognitive impairment is significantly impaired compared with that in the brain upon normal aging, and the decline in cerebral glucose metabolism occurs before pathology and symptoms manifest and gradually worsens as symptoms progress.^383–385 In 1983, de Leon et al. examined aged patients with senile dementia and found a 17–24% decline in the cerebral glucose metabolic rate.³⁸⁶ Inefficient glucose utilization, impaired ATP production, and oxidative damage are closely correlated, and these deficiencies have profound consequences in AD.^387,388 For example, ATP deficiency causes the loss of the neuronal membrane potential since Na⁺/K⁺ ATPase fails to maintain proper intracellular and extracellular gradients of Na⁺ and K⁺ ions. In addition, the propagation of action potentials and the production of neurotransmission is hindered by energy insufficiency. Moreover, after membrane depolarization (mainly due to the dissipation of Na⁺ and K⁺ ion gradients), Ca²⁺ flows down the steep gradient (~1.2 mM of extracellular Ca²⁺ to ~0.1 μM of intracellular Ca²⁺) into the cell to raise intracellular Ca²⁺ levels and stimulates the activities of various Ca²⁺-dependent enzymes (including endonucleases, phospholipases, and proteinases), eventually contributing to neuronal dysfunction and death.¹⁵⁸ Mitochondria are the most energetically and metabolically active organelles in the cell.^389,390 Mitochondria are also dynamic organelles; they experiences changes in their functional capacities, morphologies, and positions^391–393 so that they can be transported, and they respond to physiological signals to meet the energy and metabolic demands of cellular activities.^394–396 In addition to neuronal atrophy, mitochondrial dysfunction leads to hypometabolism, which in turn contributes to the progression of AD.^397–399 Indeed, there is evidence that hypometabolism and neuronal atrophy coexists in patients with amyloid-negative AD.⁴⁰⁰ In addition to mitochondrial dysfunction, hypoperfusion and hypoxia in vascular diseases may also cause hypometabolism in the brain and thus contribute to the progression of AD (Fig. 5). Meanwhile, as the synthesis of acetylcholine requires the involvement of acetyl-CoA and ATP, hypometabolism leads to a decrease in acetylcholine synthesis in neurons, which suggests that hypometabolism may be an underlying explanation for the acetylcholine hypothesis (Fig. 5).

Fig. 5.

In addition to mitochondrial dysfunction, hypometabolism may underlie the cholinergic hypothesis, metal ion hypothesis, and neurovascular hypothesis. a Glucose is enzymatically catalyzed to produce pyruvate. Pyruvate is converted to acetyl-CoA and then enters the TCA cycle or is used in the cytoplasm to synthesize acetylcholine. However, in AD patients, because of hypometabolism, the production of acetyl-CoA and ATP is insufficient, which leads to a reduction in acetylcholine synthesis. b Mitochondrial complexes I–III require Fe/S clusters, and complexes II–IV need hemoproteins for electron transfer and the oxidative phosphorylation of the respiratory chain. When iron deficiency occurs, the production of Fe/S and hemoproteins decreases, thereby affecting mitochondrial function and resulting in hypometabolism. In addition, copper is essential for the function of complex IV. Clearly, Cu–Zn superoxide dismutase (SOD1) requires copper and zinc.^455,456 c Hypoperfusion and hypoxia in vascular diseases leads to insufficient oxygen supply, which in turn leads to insufficient ATP synthesis, resulting in hypometabolism in AD patients. TCA: tricarboxylic acid cycle; SOD1: superoxide dismutase 1

The relationship between hypometabolism and autophagy in neurons is still unknown,³⁵² but calorie restriction (CR) is known to enhance autophagy. CR-induced autophagy can recycle intracellular degraded components and aggregates to maintain mitochondrial function.⁴⁰¹ Hypometabolism and a simultaneous decrease in autophagy can worsen the situation and lead to the dysfunction and atrophy of neurons. Hypometabolism and a simultaneous decrease in autophagy may be causative factors of brain atrophy and AD (Fig. 6).

Fig. 6.

Hypometabolism and autophagy decline are likely to be causative factors of neuronal atrophy. Normal neurons vs. atrophic neurons. Upper: Normal levels of autophagy and metabolism exist in neurons to maintain their morphology and function. Lower: Hypometabolism and a reduction in autophagy are found in atrophic neurons

Perspective

AD, like the aging population, has increasingly become a medical and social concern. There are currently four clinically used drugs (a total of five therapies, the fifth one of which is a combination of two drugs) that have been approved by the FDA, but they only treat the symptoms and have no significant effect on the progression of AD. Based on this retrospective review of AD and the lessons learned, we propose that fluoxetine,⁴⁰² a selective serotonin reuptake inhibitor (SSRI), may have strong potential for the treatment of AD (Fig. 7).

Fig. 7.

The potential mechanisms of fluoxetine in the remission of AD. As a selective 5-HT reuptake inhibitor, fluoxetine can increase the extraneuronal concentration of 5-HT. 5-HT binds to the 5-HT₄A receptor to promote neuronal dematuration through a Gs-mediated pathway. 5-HT binds to the 5-HT₁A receptor, which is involved in BDNF-dependent neurogenesis through the Gi-mediated signaling pathway. After 5-HT stimulation, MeCP2 is phosphorylated at Ser421 through CaMKII-dependent signaling, and this promotes the dissociation of CREB from HDAC and then increases the expression of BDNF. BDNF activates downstream signaling pathways, including the MEK-ERK pathway, which might promote the activity of α-secretase, inhibit γ-secretase, and reduce the production of toxic amyloid Aβ. Moreover, the serotonylation of histone H3 at glutamine 5 (Q5) enhances the binding of H3K4me3 and TFIID and allows gene expression. Fluoxetine has been reported to bind and inhibit NMDA receptors directly, which can reduce the inhibition of α-secretase and thus prevent the production of Aβ. In addition, fluoxetine can bind to the endoplasmic reticulum protein sigma-1 receptor, which induces the dissociation of Bip from the sigma-1 receptor and promotes neuroprotection. 5-HT: serotonin; ER: endoplasmic reticulum

Based on functional brain imaging with PET, there is evidence that serotonin plays an important role in aging, late-life depression, and AD.⁴⁰³ Short-term treatment with the antidepressant fluoxetine can trigger pyramidal dendritic spine synapse formation in the rat hippocampus.⁴⁰⁴ In an MRI study of fluoxetine for the treatment of major depression, Vakili et al. found that female responders had a statistically significant higher mean right hippocampal volume than that of nonresponders.⁴⁰⁵ Long-term treatment with fluoxetine can promote the neurogenesis and proliferation of hippocampal neurons in mice through the 5-HT₁A receptor, and this can relieve anxiety phenotypes in mice⁴⁰⁶ and enhance mitochondrial motility.⁴⁰⁷ 5-HT₄A receptors that are expressed by mature neurons in the hippocampal dentate gyrus are also important for promoting neurogenesis and dematuration.^408–410 Fluoxetine can promote neurogenesis not only in the hippocampus but also in the anterior cortex and hypothalamus.⁴¹¹ This action depends on BDNF, as fluoxetine can enhance the phosphorylation of methyl-CpG binding protein 2 (MeCP2) at serine 421 to relieve its transcriptional inhibition and thereby promote the expression of BDNF.^412,413 In addition to promoting neurite outgrowth and neurogenesis, enhanced BDNF signaling can rearrange the subcellular distribution of α-secretase, which increases its binding to APP peptides; in addition, the activity of β-secretase is inhibited after BDNF treatment.⁴¹⁴ Moreover, the serotonylation of glutamine (at position 5) in histone H3 by a transglutaminase 2-mediated manner is a sign of permissive gene expression.⁴¹⁵

Furthermore, fluoxetine has been reported to bind and inhibit NMDA receptors directly in the CNS,⁴¹⁶ and this can reduce the inhibition of α-secretase and thus prevent the production of Aβ.^203,417 Fluoxetine also inhibits γ-secretase activity and reduces the production of toxic amyloid Aβ by activating MEK-ERK signaling.^371,372 In addition, fluoxetine can bind to the endoplasmic reticulum protein sigma-1 receptor.⁴¹⁸ Sigma-1 receptor ligands can enhance acetylcholine secretion.^419,420 The sigma-1 receptor activator Anavex 2–73 has entered a phase III clinical trial after it was granted fast-track status by the FDA because of the promising results in phase II. The sigma-1 receptor is located in the mitochondrion-associated ER membrane so that the activation of the sigma-1 receptor can prolong Ca²⁺ signaling in mitochondria.⁴²¹ Consequently, the local and specific elevation of [Ca²⁺] in the mitochondrial matrix can enhance ATP synthesis,^422,423 which ameliorates hypometabolism.

In addition, our group examined the effect of SSRIs on cognitive function in AD by conducting a meta-analysis of randomized controlled studies. Of the 854 articles identified, 14 articles that involved 1091 participants were eligible for inclusion. We compared changes in MMSE scores between SSRI treatment groups and the placebo group, and we found that SSRIs may contribute to improved cognitive function, with a mean difference (MD) of 0.84 (95%CI: 0.32–1.37, P = 0.002) compared with the control. Further subgroup analysis exploring the effect of fluoxetine and other SSRIs revealed a beneficial effect of fluoxetine (MD = 1.16, 95%CI: 0.41–1.90, P = 0.002) but no benefit of other SSRIs (MD = 0.58, 95%CI: −0.17–1.33, P = 0.13) on cognitive function.⁴²⁴ Consequently, all of the above evidence indicates that fluoxetine has strong potential for the treatment of AD. In addition, because of above wealthy supporting documentation and the weak role of other SSRIs such as escitalopram in promoting BDNF release,⁴²⁵ fluoxetine was singled out as a potential therapy for the treatment of AD, not just as a complementary treatment.⁴²⁶ As summarized and illustrated in Fig. 7, the exact mechanisms of the effects of fluoxetine remain to be further clarified.

Finally, to summarize this review of the history and progress of hypotheses and clinical trials for AD, the most perplexing question is in regards to amyloid hypothesis and its failed clinical trials, which account for 22.3% of all clinical trials (Fig. 1). Although mutations in APP, PSEN1, or PSEN2 only account for ~0.5% of all AD cases,¹¹ mutations in PSEN1, which is the most common known genetic cause of FD and functions as the catalytic subunit of γ-secretase,^427,428 may cast light upon Aβ and its paradox. In 2017, Sun et al. analyzed the effect of 138 pathogenic mutations in PSEN1 on the production of Aβ−42 and Aβ−40 peptides by γ-secretase in vitro; they found that 90% of these mutations led to a decrease in the production of Aβ−42 and Aβ−40 and that 10% of these mutations result in decreased Aβ−42/Aβ−40 ratios.⁴²⁹ This comprehensive assessment of the impact of FD mutations on γ-secretase activity and Aβ production does not support the amyloid hypothesis and suggests an alternative therapeutic strategy aimed at restoring γ-secretase activity⁴³⁰; this is also supported by the fact that the functional loss of both PSEN1 and PSEN2 in the mouse postnatal forebrain causes memory impairment in an age-dependent manner.⁴³¹ Considering that the activation of Notch signaling by the cleavage of γ-secretase⁴³² is not involved in age-related neurodegeneration,⁴³³ other signaling pathways mediated by Aβ and/or other products of γ-secretase substrates, such as ErbB4,⁴³⁴ E-cadherin,⁴³⁵ N-cadherin,⁴³⁶ ephrin-B2,⁴³⁷ CD44,⁴³⁸ and LDL receptor-related protein,⁴³⁹ may play active roles in neuronal survival in the adult brain.

The most interesting and challenging phenomena regarding fluoxetine is that fluoxetine is clinically more effective in women than in men⁴⁴⁰ and that the prevalence of AD and other dementias is higher in women than in men⁴⁴¹; meanwhile, women live significantly longer than men.⁴⁴² These phenomena suggest that there are interplays or trade-offs between AD and longevity. In particular, APOE is the strongest genetic risk factor for AD^18–21 and is the only gene associated with longevity that achieves genome-wide significance (P < 5 × 10^–8).⁴⁴³ APOE4 is associated with a risk of AD that declines after the age of 70; the OR for APOE4 heterozygotes remains above unity at almost all ages; surprisingly, however, the OR for APOE4 homozygotes dips below unity after the age of 89.⁴⁴⁴ There may be genetic and nongenetic factors that interact with APOE4, lead to shorter survival in more aggressive form of AD, or promote longevity in an age-dependent manner.¹¹ Uncovering the puzzle of APOE4 and the mystery of longevity may provide insights for AD prevention.

Competing interests

The authors declare no competing interests.